normal swine serum blocking solution  (Vector Laboratories)

Journal: Frontiers in Immunology

Article Title: Porcine non-conventional B-1-like cells are a potent source of Streptococcus suis -binding IgM

doi: 10.3389/fimmu.2024.1495359

Figure Lengend Snippet: S. suis -binding serum IgM increases between four and eight weeks of age and restricts bacterial survival in vitro . (A) S. suis cps 2-binding serum IgM and IgG were analyzed in the same pigs at four and eight weeks of age by ELISA. Plates were coated with whole inactivated bacteria of S. suis . A hyperimmune serum pool defined to include 100 ELISA units served as internal reference. Data of one out of two independent experiments are shown and presented with group medians. Statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. (two-tailed, *p<0.05). (B) Survival of S. suis cps 2 strain 10 was assessed in the blood drawn from eight-weeks-old pigs in the presence/absence of the specific IgM protease Ide Ssuis . The survival factor represents the ratio of CFU at 120 min to CFU at time zero. Survival factors above one (dotted line) indicate proliferation of S. suis . Data of one out of two independent experiments are shown. Each dot represents one individual pig. Statistical analysis was performed using paired t-test. (two-tailed, *p<0.05).

Article Snippet: Swine IgM whole molecule serum (Rockland Immunochemicals, Limerick, Pottstown) was used as a standard and total IgM concentrations were calculated based on the standard curve.

Techniques: Binding Assay, In Vitro, Enzyme-linked Immunosorbent Assay, Bacteria, Two Tailed Test