svec4 10 cells svec  (ATCC)

Journal: Journal of Lipid Research

Article Title: Small dense HDLs display potent vasorelaxing activity, reflecting their elevated content of sphingosine-1-phosphate

doi: 10.1194/jlr.M076927

Figure Lengend Snippet: A: Kinetics of NO formation by murine endothelial cells (SVECs) treated with total HDL (50 μg protein per milliliter). B: NO formation by SVECs treated with total HDL and HDL3c (50 μg protein per milliliter) alone or in combination with the S1P receptor antagonist, VPC 23019 (VPC). CRL2181 cells were preincubated with VPC (10 μM) for 30 min before adding HDL. A fluorescent probe for NO [DAF-2DA (5 μM in DMSO)] was preincubated for 30 min with cells before adding HDL. The fluorescent intensity was recorded at the excitation wavelength of 485 nm and emission wavelength of 520 nm and presented as a percentage of baseline reading obtained at time 0 (F0) (A) or in the absence of HDL (F-control) (B). Data shown are means of four independent experiments. *P < 0.05 versus time 0 (A) or versus corresponding HDL in the absence of VPC (B).

Article Snippet: NO production in endothelial cells Simian vacuolating virus 40 (SV40)-transformed murine endothelial cells (SVECs) (ATCC number: CRL2181) were cultured in DMEM supplemented with 10% FBS to sub-confluency.

Techniques: Control