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surescan microarray scanner  (Agilent technologies)


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    Agilent technologies surescan microarray scanner
    Surescan Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/surescan+microarray+scanner/10__1016_slash_j__bbrep__2024__101814-93-58-58
    Average 90 stars, based on 1 article reviews
    surescan microarray scanner - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Purification:

    Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue
    Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Agilent SureScan Microarray Scanner and Agilent Scan Control software (version 9.1.3). .. The microarray scan images were extracted using the Agilent Feature Extraction Software.

    Microarray:

    Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue
    Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Agilent SureScan Microarray Scanner and Agilent Scan Control software (version 9.1.3). .. The microarray scan images were extracted using the Agilent Feature Extraction Software.

    Article Title: YAP/TAZ, beta-catenin, and TGFb pathway activation in medical plasma-induced wound healing in diabetic mice.
    Article Snippet: After purification and quantification of cRNA, samples were blocked and hybridized onto SurePrint G3 custom GE 8 60 K chips (OakLabs, Germany) for 17 h at 65 C in a hybridization oven (Agilent, USA) following previously established procedures [43]. .. After extensive washing steps in several wash buffers, fluorescence signals were measured using an Agilent SureScan microarray scanner. .. Using Agilent Feature Extraction Software, signal intensities were analyzed and normalized, and downstream analysis of Agilent microarray data, such as statistical comparison and hierarchical clustering, was done using Genespring software (Agilent, Germany).

    Article Title: Inverted triplications formed by iterative template switches generate structural variant diversity at genomic disorder loci
    Article Snippet: The mixture was placed on the appropriate array and placed in a revolving hybridization chamber at 65°C for 40 h. After hybridization the arrays were washed with Agilent OligoCGH Wash buffers 1 and 2. .. Slides were scanned using the Agilent SureScan Microarray Scanner and resulting image processed using the Agilent feature extraction software. .. Coordinates for each CNV observed along those chromosomes were annotated using the Agilent Genomic Workbench software.

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Article Title: Physicochemical Properties of Different Sulfated Polysaccharide Components from Laetiporus sulphureus and Their Anti-Proliferative Effects on MDA-MB-231 Breast Cancer Cells
    Article Snippet: .. The microarray chips were incubated with biotin-labeled proteins at room temperature for 2 h, followed by incubating with a Cy3-conjugated streptavidin dye for 1 h. The fluorescence intensity of the chip was detected by SureScan Microarray Scanner (Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Effects of prolonged application of peracetic acid-based disinfectant on recirculating aquaculture systems stocked with Atlantic salmon parr.
    Article Snippet: • Two doses of PAA (0.1 and 1 mg/L) were assessed in RAS with salmon parr • No differences among control and PAA were found in growth after 28 days • Biological and chemical assessment of oxidative stress showed same results • A PAA of 0.1 mg/L resulted in more differentially expressed genes in olfac-

    Article Title: Method for diagnosis based on circulating extracellular vesicles
    Article Snippet: .. After washed with PBST (0.05%) to remove unconjugated EVs and antibodies, the slide was scanned with two wavelengths (532 and 640 nm) via a laser-induced fluorescence scanner, such as Agilent SureScan microarray scanner or GenePix Microarray Scanner, to detect fluorescent signal from detection antibodies. ..

    Article Title: Revealing the potential role of hsa-miR-663a in modulating the PI3K-Akt signaling pathway via miRNA microarray in spinal muscular atrophy patient fibroblast-derived iPSCs.
    Article Snippet: .. The array was analyzed using Agilent SureScan Microarray Scanner (G4900DA). .. Normalized intensities were extracted using the Agilent Feature Extraction Software and the analysis were performed using Agilent GeneSpring Analysis Software version 14.9.1.

    Hybridization:

    Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue
    Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Agilent SureScan Microarray Scanner and Agilent Scan Control software (version 9.1.3). .. The microarray scan images were extracted using the Agilent Feature Extraction Software.

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Article Title: Effects of prolonged application of peracetic acid-based disinfectant on recirculating aquaculture systems stocked with Atlantic salmon parr.
    Article Snippet: • Two doses of PAA (0.1 and 1 mg/L) were assessed in RAS with salmon parr • No differences among control and PAA were found in growth after 28 days • Biological and chemical assessment of oxidative stress showed same results • A PAA of 0.1 mg/L resulted in more differentially expressed genes in olfac-

    Gene Expression:

    Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue
    Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Agilent SureScan Microarray Scanner and Agilent Scan Control software (version 9.1.3). .. The microarray scan images were extracted using the Agilent Feature Extraction Software.

    Article Title: Effects of prolonged application of peracetic acid-based disinfectant on recirculating aquaculture systems stocked with Atlantic salmon parr.
    Article Snippet: • Two doses of PAA (0.1 and 1 mg/L) were assessed in RAS with salmon parr • No differences among control and PAA were found in growth after 28 days • Biological and chemical assessment of oxidative stress showed same results • A PAA of 0.1 mg/L resulted in more differentially expressed genes in olfac-

    Control:

    Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue
    Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Agilent SureScan Microarray Scanner and Agilent Scan Control software (version 9.1.3). .. The microarray scan images were extracted using the Agilent Feature Extraction Software.

    Software:

    Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue
    Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Agilent SureScan Microarray Scanner and Agilent Scan Control software (version 9.1.3). .. The microarray scan images were extracted using the Agilent Feature Extraction Software.

    Article Title: Inverted triplications formed by iterative template switches generate structural variant diversity at genomic disorder loci
    Article Snippet: The mixture was placed on the appropriate array and placed in a revolving hybridization chamber at 65°C for 40 h. After hybridization the arrays were washed with Agilent OligoCGH Wash buffers 1 and 2. .. Slides were scanned using the Agilent SureScan Microarray Scanner and resulting image processed using the Agilent feature extraction software. .. Coordinates for each CNV observed along those chromosomes were annotated using the Agilent Genomic Workbench software.

    Article Title: Effects of prolonged application of peracetic acid-based disinfectant on recirculating aquaculture systems stocked with Atlantic salmon parr.
    Article Snippet: • Two doses of PAA (0.1 and 1 mg/L) were assessed in RAS with salmon parr • No differences among control and PAA were found in growth after 28 days • Biological and chemical assessment of oxidative stress showed same results • A PAA of 0.1 mg/L resulted in more differentially expressed genes in olfac-

    Fluorescence:

    Article Title: YAP/TAZ, beta-catenin, and TGFb pathway activation in medical plasma-induced wound healing in diabetic mice.
    Article Snippet: After purification and quantification of cRNA, samples were blocked and hybridized onto SurePrint G3 custom GE 8 60 K chips (OakLabs, Germany) for 17 h at 65 C in a hybridization oven (Agilent, USA) following previously established procedures [43]. .. After extensive washing steps in several wash buffers, fluorescence signals were measured using an Agilent SureScan microarray scanner. .. Using Agilent Feature Extraction Software, signal intensities were analyzed and normalized, and downstream analysis of Agilent microarray data, such as statistical comparison and hierarchical clustering, was done using Genespring software (Agilent, Germany).

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Article Title: Physicochemical Properties of Different Sulfated Polysaccharide Components from Laetiporus sulphureus and Their Anti-Proliferative Effects on MDA-MB-231 Breast Cancer Cells
    Article Snippet: .. The microarray chips were incubated with biotin-labeled proteins at room temperature for 2 h, followed by incubating with a Cy3-conjugated streptavidin dye for 1 h. The fluorescence intensity of the chip was detected by SureScan Microarray Scanner (Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Method for diagnosis based on circulating extracellular vesicles
    Article Snippet: .. After washed with PBST (0.05%) to remove unconjugated EVs and antibodies, the slide was scanned with two wavelengths (532 and 640 nm) via a laser-induced fluorescence scanner, such as Agilent SureScan microarray scanner or GenePix Microarray Scanner, to detect fluorescent signal from detection antibodies. ..

    Extraction:

    Article Title: Inverted triplications formed by iterative template switches generate structural variant diversity at genomic disorder loci
    Article Snippet: The mixture was placed on the appropriate array and placed in a revolving hybridization chamber at 65°C for 40 h. After hybridization the arrays were washed with Agilent OligoCGH Wash buffers 1 and 2. .. Slides were scanned using the Agilent SureScan Microarray Scanner and resulting image processed using the Agilent feature extraction software. .. Coordinates for each CNV observed along those chromosomes were annotated using the Agilent Genomic Workbench software.

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Incubation:

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Article Title: Physicochemical Properties of Different Sulfated Polysaccharide Components from Laetiporus sulphureus and Their Anti-Proliferative Effects on MDA-MB-231 Breast Cancer Cells
    Article Snippet: .. The microarray chips were incubated with biotin-labeled proteins at room temperature for 2 h, followed by incubating with a Cy3-conjugated streptavidin dye for 1 h. The fluorescence intensity of the chip was detected by SureScan Microarray Scanner (Agilent Technologies, Santa Clara, CA, USA). ..



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    Image Search Results


    Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Journal: bioRxiv

    Article Title: Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis

    doi: 10.1101/2024.06.24.600143

    Figure Lengend Snippet: Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Article Snippet: Following hybridization, the array slides were washed and scanned with an Agilent SureScan High-Resolution DNA Microarray Scanner.

    Techniques: Microarray, Software