surescan microarray scanner (Agilent technologies)
Structured Review
Surescan Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/surescan+microarray+scanner/10__1016_slash_j__bbrep__2024__101814-93-58-58
Average 90 stars, based on 1 article reviews
Images
Related Articles
Purification:Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Microarray:Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Article Title: YAP/TAZ, beta-catenin, and TGFb pathway activation in medical plasma-induced wound healing in diabetic mice. Article Snippet: After purification and quantification of cRNA, samples were blocked and hybridized onto SurePrint G3 custom GE 8 60 K chips (OakLabs, Germany) for 17 h at 65 C in a hybridization oven (Agilent, USA) following previously established procedures [43]. .. After extensive washing steps in several wash buffers, fluorescence signals were measured using an Article Title: Inverted triplications formed by iterative template switches generate structural variant diversity at genomic disorder loci Article Snippet: The mixture was placed on the appropriate array and placed in a revolving hybridization chamber at 65°C for 40 h. After hybridization the arrays were washed with Agilent OligoCGH Wash buffers 1 and 2. .. Slides were scanned using the Article Title: Serum miR‑29 is increased in mice with early liver fibrosis Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a Article Title: Physicochemical Properties of Different Sulfated Polysaccharide Components from Laetiporus sulphureus and Their Anti-Proliferative Effects on MDA-MB-231 Breast Cancer Cells Article Snippet: .. The microarray chips were incubated with biotin-labeled proteins at room temperature for 2 h, followed by incubating with a Cy3-conjugated streptavidin dye for 1 h. The fluorescence intensity of the chip was detected by Article Title: Effects of prolonged application of peracetic acid-based disinfectant on recirculating aquaculture systems stocked with Atlantic salmon parr. Article Snippet: • Two doses of PAA (0.1 and 1 mg/L) were assessed in RAS with salmon parr • No differences among control and PAA were found in growth after 28 days • Biological and chemical assessment of oxidative stress showed same results • A PAA of 0.1 mg/L resulted in more differentially expressed genes in olfac- Article Title: Method for diagnosis based on circulating extracellular vesicles Article Snippet: .. After washed with PBST (0.05%) to remove unconjugated EVs and antibodies, the slide was scanned with two wavelengths (532 and 640 nm) via a laser-induced fluorescence scanner, such as Article Title: Revealing the potential role of hsa-miR-663a in modulating the PI3K-Akt signaling pathway via miRNA microarray in spinal muscular atrophy patient fibroblast-derived iPSCs. Article Snippet: .. The array was analyzed using Hybridization:Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Article Title: Serum miR‑29 is increased in mice with early liver fibrosis Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a Article Title: Effects of prolonged application of peracetic acid-based disinfectant on recirculating aquaculture systems stocked with Atlantic salmon parr. Article Snippet: • Two doses of PAA (0.1 and 1 mg/L) were assessed in RAS with salmon parr • No differences among control and PAA were found in growth after 28 days • Biological and chemical assessment of oxidative stress showed same results • A PAA of 0.1 mg/L resulted in more differentially expressed genes in olfac- Gene Expression:Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Article Title: Effects of prolonged application of peracetic acid-based disinfectant on recirculating aquaculture systems stocked with Atlantic salmon parr. Article Snippet: • Two doses of PAA (0.1 and 1 mg/L) were assessed in RAS with salmon parr • No differences among control and PAA were found in growth after 28 days • Biological and chemical assessment of oxidative stress showed same results • A PAA of 0.1 mg/L resulted in more differentially expressed genes in olfac- Control:Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Software:Article Title: In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue Article Snippet: .. The cRNA was further purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), fragmented at 60 ◦C for 30 min, and loaded onto the microarray slide for hybridization at 65 ◦C for 17 h. After hybridization, the slide was washed with Gene Expression Wash Buffer I and II to remove any non-hybridized cRNA and scanned immediately using the Article Title: Inverted triplications formed by iterative template switches generate structural variant diversity at genomic disorder loci Article Snippet: The mixture was placed on the appropriate array and placed in a revolving hybridization chamber at 65°C for 40 h. After hybridization the arrays were washed with Agilent OligoCGH Wash buffers 1 and 2. .. Slides were scanned using the Article Title: Effects of prolonged application of peracetic acid-based disinfectant on recirculating aquaculture systems stocked with Atlantic salmon parr. Article Snippet: • Two doses of PAA (0.1 and 1 mg/L) were assessed in RAS with salmon parr • No differences among control and PAA were found in growth after 28 days • Biological and chemical assessment of oxidative stress showed same results • A PAA of 0.1 mg/L resulted in more differentially expressed genes in olfac- Fluorescence:Article Title: YAP/TAZ, beta-catenin, and TGFb pathway activation in medical plasma-induced wound healing in diabetic mice. Article Snippet: After purification and quantification of cRNA, samples were blocked and hybridized onto SurePrint G3 custom GE 8 60 K chips (OakLabs, Germany) for 17 h at 65 C in a hybridization oven (Agilent, USA) following previously established procedures [43]. .. After extensive washing steps in several wash buffers, fluorescence signals were measured using an Article Title: Serum miR‑29 is increased in mice with early liver fibrosis Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a Article Title: Physicochemical Properties of Different Sulfated Polysaccharide Components from Laetiporus sulphureus and Their Anti-Proliferative Effects on MDA-MB-231 Breast Cancer Cells Article Snippet: .. The microarray chips were incubated with biotin-labeled proteins at room temperature for 2 h, followed by incubating with a Cy3-conjugated streptavidin dye for 1 h. The fluorescence intensity of the chip was detected by Article Title: Method for diagnosis based on circulating extracellular vesicles Article Snippet: .. After washed with PBST (0.05%) to remove unconjugated EVs and antibodies, the slide was scanned with two wavelengths (532 and 640 nm) via a laser-induced fluorescence scanner, such as Extraction:Article Title: Inverted triplications formed by iterative template switches generate structural variant diversity at genomic disorder loci Article Snippet: The mixture was placed on the appropriate array and placed in a revolving hybridization chamber at 65°C for 40 h. After hybridization the arrays were washed with Agilent OligoCGH Wash buffers 1 and 2. .. Slides were scanned using the Article Title: Serum miR‑29 is increased in mice with early liver fibrosis Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a Incubation:Article Title: Serum miR‑29 is increased in mice with early liver fibrosis Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a Article Title: Physicochemical Properties of Different Sulfated Polysaccharide Components from Laetiporus sulphureus and Their Anti-Proliferative Effects on MDA-MB-231 Breast Cancer Cells Article Snippet: .. The microarray chips were incubated with biotin-labeled proteins at room temperature for 2 h, followed by incubating with a Cy3-conjugated streptavidin dye for 1 h. The fluorescence intensity of the chip was detected by |
