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supt1 cells  (ATCC)


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    ATCC supt1 cells
    Supt1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/supt1+cells/SUP-T1/pm42272105-128-0-2
    Average 97 stars, based on 609 article reviews
    supt1 cells - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: DNA Damage Response Proteins Are Involved in the Formation of Defective HIV-1 Proviruses
    Article Snippet: Following overnight incubation at 37°C, bacterial colonies were scraped with cold LB broth (Sigma-Aldrich, L3022) and directly used for DNA isolation by QIAGEN Plasmid Maxi Kit (Qiagen, 12165). .. SupT1 cells, (( ) kindly provided by Dharam Ablashi through the NIH AIDS Reagent Programme, Division of AIDS, NIAID, NIH) and K562 cells (American Type Culture Collection, CCL-243 TM) were cultured in RPMI-1640 (Sigma-Aldrich, R8758) supplemented with 10% (v/v) fetal bovine serum (FBS) (Thermo Fisher Scientific, A5256701), 100 U/ml Penicillin (GibcoTM, 15140122), and 100 μg/ml Streptomycin (GibcoTM, 15140122). .. K562-VPR cells (Horizon Discovery, HD dCas9-VPR-005) were cultured in IMDM (GibcoTM, 12440053) supplemented with 10% (v/v) FBS, 100 U/ml Penicillin, and 100 μg/ml Streptomycin.

    Article Title: Antibody Format Matters: A Comparative Analysis of VHH and scFv Domains Reveals Superior In Vivo CAR T Cell Function with VHH Domains.
    Article Snippet: Alexander Kinna, Preeta Datta, Reyisa Bughda, Farhaan Parekh, Mathew Robson, Ram Jha, Phillip Wu, Ian Scott, Elizabeth Hogben, Oliver Amin, Gordon Weng-Kit Cheung, Warren Hazelton, Zulaikha Akbar, Katarina Lamb, Emily Macken, Chris Allen, Sofi Panovska, Lisa Hogan, Mohamed El-Kholy, Reevesh Shrestha, Jonathan Popplewell, Evangelia Kokalaki, James Sillibourne, Simon Thomas, Shimobi Onuoha, Mathieu Ferrari, Martin Pule

    Article Title: Fc engineering of a fully humanized anti-CD147 monoclonal antibody enhances ADCC against T-cell acute lymphoblastic leukemia and T-lymphoblastic lymphoma.
    Article Snippet: .. Jurkat (clone E6-1) and SupT1 cells were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA) and cultured in Roswell Park Memorial Institute 1640 (RPMI-1640) medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mM L-glutamine. .. HEK293T cells from ATCC were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Carlsbad, CA, USA) supplemented with 10% heat-inactivated FBS (Gibco, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Carlsbad, CA, USA).

    other:

    Article Title: CXCL10-induced regulatory T cells and adenosine signaling promote immunosuppression and progression of epithelial ovarian cancer
    Article Snippet: SUPT1 cells (ATCC), a human lymphoblastic leukemia T-cell line, were grown in RPMI medium supplemented with 10% FBS and penicillin-streptomycin antibiotics.

    Article Title: CXCL10-induced regulatory T cells and adenosine signaling promote immunosuppression and progression of epithelial ovarian cancer.
    Article Snippet: SUPT1 cells (ATCC), a human lymphoblastic leukemia T-cell line, were grown in RPMI medium supplemented with 10% FBS and penicillin-streptomycin antibiotics.



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    Transduction of SupT1 (A) and Jurkat (B) cells with wild-type (wt) and CD7-engineered AAVs of different serotypes (10,000 gc/cell). Shown are relative % GFP-positive cells at 3 dpi compared to corresponding wt AAV serotype (mean ± SD, n=3). Absolute percentage GFP-positive cells are indicated inside the bars. C ) Representative flow cytometry plots of activated human PBMCs transduced with indicated rAAVs (100,000 gc/cell). D) Absolute % GFP-positivity at 3 dpi (mean ± SD, n≥4). Individual values are presented as dots. E) CD7-specificity factor for transduction (10,000 gc/cell) of activated PBMCs at 3 dpi (mean ± SD, n=3). F) Transduction of activated human PBMCs with indicated rAAVs (100,000 gc/cell) and stratification of GFP-positive cells at 3 dpi in CD4 + T, CD8 + T and NK (CD56 + ) cell types (mean ± SD, n≥3; dot colors represent biologically independent human cell samples). G) Transduction of hepatocyte (HuH-7, HepG2) and kidney (HEK293T) cell lines with indicated rAAVs (100.000 gc/cell). Absolute % GFP-positive cells at 3 dpi (mean ± SD, n=3). rAAV Vectors contained the following sequences: AAV6 & AAV2 = self-complementary genome, CAG promoter; AAV9 & AAV6/9 = single stranded genome, SFFV promoter. Mean ± SD, n=3. For all panels statistical analysis utilized unpaired t-test, p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), and p < 0.0001 (****).

    Journal: bioRxiv

    Article Title: Development of a recombinant adeno-associated virus vector for human T lymphocyte- and natural killer cell-targeted gene therapy

    doi: 10.64898/2026.02.26.707014

    Figure Lengend Snippet: Transduction of SupT1 (A) and Jurkat (B) cells with wild-type (wt) and CD7-engineered AAVs of different serotypes (10,000 gc/cell). Shown are relative % GFP-positive cells at 3 dpi compared to corresponding wt AAV serotype (mean ± SD, n=3). Absolute percentage GFP-positive cells are indicated inside the bars. C ) Representative flow cytometry plots of activated human PBMCs transduced with indicated rAAVs (100,000 gc/cell). D) Absolute % GFP-positivity at 3 dpi (mean ± SD, n≥4). Individual values are presented as dots. E) CD7-specificity factor for transduction (10,000 gc/cell) of activated PBMCs at 3 dpi (mean ± SD, n=3). F) Transduction of activated human PBMCs with indicated rAAVs (100,000 gc/cell) and stratification of GFP-positive cells at 3 dpi in CD4 + T, CD8 + T and NK (CD56 + ) cell types (mean ± SD, n≥3; dot colors represent biologically independent human cell samples). G) Transduction of hepatocyte (HuH-7, HepG2) and kidney (HEK293T) cell lines with indicated rAAVs (100.000 gc/cell). Absolute % GFP-positive cells at 3 dpi (mean ± SD, n=3). rAAV Vectors contained the following sequences: AAV6 & AAV2 = self-complementary genome, CAG promoter; AAV9 & AAV6/9 = single stranded genome, SFFV promoter. Mean ± SD, n=3. For all panels statistical analysis utilized unpaired t-test, p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), and p < 0.0001 (****).

    Article Snippet: Suspension cell lines SupT1 (ATCC: CRL-1942) and Jurkat (RRID: CVCL_C831) were maintained in RPMI 1640 (Biozym Scientific) with 10% FCS, 50 U/mL Penicillin and 50 μg/mL Streptomycin (Pan Biotech).

    Techniques: Transduction, Flow Cytometry