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1x first strand buffer  (Thermo Fisher)


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    Thermo Fisher 1x first strand buffer
    1x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+buffer/Tris+EDTA+buffer%2C+for+molecular+biology%2C+DNAse%2C+RNAse%2C+Protease+free+ready+to+use%2C+pH+8%2E0/pm42002808-95-17-21
    Average 95 stars, based on 1 article reviews
    1x first strand buffer - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Supporting Information Design, Synthesis and Characterization of N-oxide-containing Heterocycles with In vivo Sterilizing Antitubercular Activity
    Article Snippet: .. After centrifugation, for each combination, 5 μL of first strand buffer (5×, Invitrogen), 2.5 μL of dithiothreitol (DTT, 100 mM), 2.3 μL of dNTPs (5 mM dA/G/TTP and 2 mM dCTP), 1.7 μL of Cy3 or Cy5 dCTP (GE Healthcare), and 2.5 μL of the SuperScript III enzyme (200 U/μL) were added. ..

    Reverse Transcription:

    Article Title: RNA tomography reveals spatial gene expression maps of Arabidopsis thaliana roots infected with Heterodera schachtii.
    Article Snippet: RNA amplification, cDNA synthesis, and Illumina library preparation To each tissue section, 0.1 ll of 10 mM dNTPs (Promega) and 0.2 ll (1 : 500 000 diluted) ERCC spike-ins were added using the Nanodrop II robot (GC Biotech, Waddinxveen, the Netherlands). .. The plate was heated to 65°C for 5 min to release the RNA and spun down in a pre-chilled centrifuge at 20 000 g for 10 s. Next, a mix containing 0.05 ll nuclease-free water, 0.4 ll First Strand Buffer (59), 0.2 ll DTT (0.1 M), 0.1 ll RNAse OUT (40 U ll 1), and 0.1 ll SuperScript II (200 U ll 1) was prepared, and 0.75 ll of this mix was added to each well using the Nanodrop II for reverse transcription before incubation for 1 h at 42°C, 5 min at 4°C, and 10 min at 70°C. ..

    Article Title: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development
    Article Snippet: .. Then, each sample was mixed with 2.83 μL of reverse transcription mixture, comprising SuperScript IV reverse transcriptase (Invitrogen, 18090050), RNase inhibitor (Takara, Cat. 2313A), SuperScript IV first-strand buffer, 1 M betaine (Sigma-Aldrich, B0300-1VL), 100 mM DTT (Invitrogen, 18090050), 50 mM MgCl2 (Sangon, A610328-0500), and template-switching oligo primers. ..

    Article Title: Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions.
    Article Snippet: .. Then, 4 μL of the first strand buffer of M-MLV reverse transcriptase (Invitrogen), 2 μL 100mM DTT, 1 μL RNaseOut and 1μL Template-Switch primer 100μM were added to each tube. .. After incubating 2min at 37 °C 1 μL of M-MLV reverse transcriptase (Invitrogen) was added to each tube,whichwere incubated for 50minat 37 °C and 15min at 70 °C.

    Article Title: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development.
    Article Snippet: .. Then, each sample was mixed with 2.83 μL of reverse transcription mixture, comprising SuperScript IV reverse transcriptase (Invitrogen, 18090050), RNase inhibitor (Takara, Cat. 2313A), SuperScript IV first-strand buffer, 1 M betaine (Sigma-Aldrich, B0300-1VL), 100 mM DTT (Invitrogen, 18090050), 50 mM MgCl2 (Sangon, A610328-0500), and template-switching oligo primers. ..

    Article Title: STAT3 Signaling Mediates Agomelatine Restoration of Prefrontal Cortex Synaptic Plasticity in Chronic Social Defeat Stress Mice.
    Article Snippet: Total RNA was extracted from PFC tissues using TRIzol reagent (Sigma-Aldrich). .. For cDNA synthesis, 1 μg of total RNA was reverse-transcribed in a 20 μL reaction mixture containing First-Strand buffer, 0.5 μg oligo(dT) primers, 0.5 mM dNTP mix, and M-MLV reverse transcriptase (Thermo Fisher Scientific). .. Quantitative PCR was performed using SYBR Green Master Mix (PCR Biosystems) on a real-time PCR system.

    Article Title: Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions
    Article Snippet: .. Briefly, the first strand was obtained by incubating 500 ng of total RNA, 1 μL of primers mIGK RT, mIGL RT or mIGHG RT at 10 μM in separate tubes, 1 μL of 10 mM dNTPs and 11 μL of RNase-free water at 65 °C for 5 min. Then, 4 μL of the first strand buffer of M-MLV reverse transcriptase (Invitrogen), 2 μL 100 mM DTT, 1 μL RNaseOut and 1 μL Template-Switch primer 100 μM were added to each tube. .. After incubating 2 min at 37 °C 1 μL of M-MLV reverse transcriptase (Invitrogen) was added to each tube, which were incubated for 50 min at 37 °C and 15 min at 70 °C.

    Incubation:

    Article Title: RNA tomography reveals spatial gene expression maps of Arabidopsis thaliana roots infected with Heterodera schachtii.
    Article Snippet: RNA amplification, cDNA synthesis, and Illumina library preparation To each tissue section, 0.1 ll of 10 mM dNTPs (Promega) and 0.2 ll (1 : 500 000 diluted) ERCC spike-ins were added using the Nanodrop II robot (GC Biotech, Waddinxveen, the Netherlands). .. The plate was heated to 65°C for 5 min to release the RNA and spun down in a pre-chilled centrifuge at 20 000 g for 10 s. Next, a mix containing 0.05 ll nuclease-free water, 0.4 ll First Strand Buffer (59), 0.2 ll DTT (0.1 M), 0.1 ll RNAse OUT (40 U ll 1), and 0.1 ll SuperScript II (200 U ll 1) was prepared, and 0.75 ll of this mix was added to each well using the Nanodrop II for reverse transcription before incubation for 1 h at 42°C, 5 min at 4°C, and 10 min at 70°C. ..

    cDNA Synthesis:

    Article Title: STAT3 Signaling Mediates Agomelatine Restoration of Prefrontal Cortex Synaptic Plasticity in Chronic Social Defeat Stress Mice.
    Article Snippet: Total RNA was extracted from PFC tissues using TRIzol reagent (Sigma-Aldrich). .. For cDNA synthesis, 1 μg of total RNA was reverse-transcribed in a 20 μL reaction mixture containing First-Strand buffer, 0.5 μg oligo(dT) primers, 0.5 mM dNTP mix, and M-MLV reverse transcriptase (Thermo Fisher Scientific). .. Quantitative PCR was performed using SYBR Green Master Mix (PCR Biosystems) on a real-time PCR system.



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    a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release <t>mRNAs.</t> <t>First-strand</t> cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.
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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells

    doi: 10.1016/j.xpro.2025.104275

    Figure Lengend Snippet: RT mix-II

    Article Snippet: 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044.

    Techniques:

    a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release mRNAs. First-strand cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

    Journal: Nature Communications

    Article Title: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development

    doi: 10.1038/s41467-026-69176-8

    Figure Lengend Snippet: a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release mRNAs. First-strand cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

    Article Snippet: Then, each sample was mixed with 2.83 μL of reverse transcription mixture, comprising SuperScript IV reverse transcriptase (Invitrogen, 18090050), RNase inhibitor (Takara, Cat. 2313A), SuperScript IV first-strand buffer, 1 M betaine (Sigma-Aldrich, B0300-1VL), 100 mM DTT (Invitrogen, 18090050), 50 mM MgCl2 (Sangon, A610328-0500), and template-switching oligo primers.

    Techniques: Control, Synthesized, Amplification, Derivative Assay, Purification, Sequencing, Modification, Biomarker Discovery