mut s mgrb truncated strain backgrounds  (ATCC)

Journal: Nucleic Acids Research

Article Title: PhoP-regulated VirK acts as an accessory factor to maintain virulence in polymyxin-resistant Klebsiella pneumoniae

doi: 10.1093/nar/gkag290

Figure Lengend Snippet: Transcriptomic analysis of Mut-S in comparison to Kpn2146 strains. ( A ) Volcano map displaying the upregulated (red dots) and downregulated (yellow dots) genes between the Mut-S group and the control group. ( B ) Changes in the relative expression of the mgrB, phoP , and virK genes in the Mut-S strain compared with those in the WT strain. ( C )Changes in the virK gene expression in the Mut-S strain after supplementation with the mgrB gene. ( D ) Changes in phoP and virK gene expressions after phoP gene knockdown in the Mut-S strain. ( E ) Changes in the mgrB and virK gene expressions after the mgrB gene was complemented in the phoP knockout strain in Mut-S. ( F ) Changes in the phoP and virK gene expressions after the phoP gene was supplemented in the phoP knockout strains in Mut-S. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: The effects of virK on bacterial pathogenicity were further evaluated in a mouse systemic infection model using virK mutants and complemented strains under K. pneumoniae ATCC BAA2146 (wild type) or Mut-S ( mgrB truncated strain) backgrounds.

Techniques: Comparison, Control, Expressing, Gene Expression, Knockdown, Knock-Out

Journal: bioRxiv

Article Title: Positive allosteric modulator of SERCA pump NDC-1171 attenuates cardiac functional decline in mouse model of Duchenne muscular dystrophy

doi: 10.64898/2026.03.05.709950

Figure Lengend Snippet: Study design outlining treatment of DBA/2J (control), D2. mdx vehicle, and D2. mdx NDC□1171 groups (n = 9 per group). Mice received oral gavage from 6 to 14 weeks of age. Ultrasound transducer icons denote timepoints at which transthoracic echocardiography wa performed (baseline: 6, midpoint: 11, endpoint: 14, and post-behavior: 16 weeks of age; n□=□5–6 per group). Behavioral testing icon represents the functional behavior testing (treadmill exhaustion and four□limb grip strength) conducted at week 15. Mice were euthanized at week 17 and cardiac tissue was collected for histological evaluation.

Article Snippet: Male D2. mdx mice (n=18, strain #013141, D2.B10-Dmdmdx/J) and its background strain DBA/2J mice (Control) were obtained from Jackson Laboratory at 4 weeks of age (n=9).

Techniques: Control, Functional Assay

Journal: bioRxiv

Article Title: Positive allosteric modulator of SERCA pump NDC-1171 attenuates cardiac functional decline in mouse model of Duchenne muscular dystrophy

doi: 10.64898/2026.03.05.709950

Figure Lengend Snippet: D2. mdx vehicle-treated mice (Vehicle) had impairments when compared to DBA/2J vehicle-treated (Control) in both (A) Four-limb grip strength test and (B) Treadmill exhaustion test. D2. mdx mice treated with NDC-1171 did not improve in either behavior assessments. * p <0.05, ** p <0.01, and *** p <0.001.

Article Snippet: Male D2. mdx mice (n=18, strain #013141, D2.B10-Dmdmdx/J) and its background strain DBA/2J mice (Control) were obtained from Jackson Laboratory at 4 weeks of age (n=9).

Techniques: Control