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nikon stedycon microscope operating  (Nikon)


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    Structured Review

    Nikon nikon stedycon microscope operating
    Nikon Stedycon Microscope Operating, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stedycon/Objectives/pm40586369-242-8-8
    Average 99 stars, based on 59420 article reviews
    nikon stedycon microscope operating - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Microscopy:

    Article Title: Tetraspanner-based nanodomains modulate BAR domain-induced membrane curvature.
    Article Snippet: For STED imaging proteins of interest were genetically fused to the HaloTag (Halo) and subsequently labeled with Janelia Fluor® 646 HaloTag ligand (#GA1121, Promega) by incubating 200 nM ligand (from 200 μM stock in DMSO) with cells in SCD medium at 30°C for 30–120 min. STED super-resolution imaging was performed on a STEDYCON scanner with pulsed 450 nm (confocal), 640 nm excitation lasers and a pulsed 775 nm depletion laser (Abberior Instruments GmbH, Göttingen, Germany). .. The STEDYCON was attached to a Nikon Eclipse Ti-E microscope with a 100×/1.45 NA oil immersion objective. ..

    Article Title: Stress in utero: prenatal dexamethasone exposure causes greater structural gliovascular alterations in female offspring than in males
    Article Snippet: Whole slide imaging was acquired using a Slide scanner (Carl Zeiss Axio Scan.Z1) equipped with a 20x objective (Plan-Apochromat 20x/0.8 M27), capturing the maximum z-stack images feasible (scaling per pixel 0.325 μm x 0.325 μm x 2.500 μm). .. For high resolution imagens slices were mounted in liquid antifade Abberior Mount Medium (Abberior, MM-2009-1ML) and 2D immunofluorescence images were acquired on an Abberior Stimulated Emission Depletion (STED) microscope equipped with a STEDYCON, equipped with 100x oil immersion objective (Nikon Plan Apo 100x/ 1.45 oil, w.d: 0.13 mm). ..

    Article Title: Identification of a nanobody able to catalyze the destruction of the spike-trimer of SARS-CoV-2.
    Article Snippet: Neutralizing antibodies have been designed to specifically target and bind to the receptor binding domain (RBD) of spike (S) protein to block severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus from attaching to angiotensin converting enzyme 2 (ACE2).. This study reports a distinctive nanobody, designated as VHH21, that directly catalyzes the S-trimer into an irreversible transition state through postfusion conformational changes.. Derived from camels immunized with multiple antigens, a set of nanobodies with high affinity for the S1 protein displays abilities to neutralize pseudovirion infections with a broad resistance to variants of concern of SARS-CoV-2, including SARS-CoV and BatRaTG13.

    Article Title: Role of α2δ-3 in regulating calcium channel localization at presynaptic active zones during homeostatic plasticity
    Article Snippet: .. STED images of single 1b boutons (three most distal boutons per synapse) were obtained from the NMJ on muscle 6/7 segment A2 using a Nikon Eclipse Ti2-E confocal microscope equipped with an Abberior STEDYCON unit and a Nikon Plan Apo 100× NA1.45 Lambda Oil DIC N2 objective. ..

    Immunofluorescence:

    Article Title: Stress in utero: prenatal dexamethasone exposure causes greater structural gliovascular alterations in female offspring than in males
    Article Snippet: Whole slide imaging was acquired using a Slide scanner (Carl Zeiss Axio Scan.Z1) equipped with a 20x objective (Plan-Apochromat 20x/0.8 M27), capturing the maximum z-stack images feasible (scaling per pixel 0.325 μm x 0.325 μm x 2.500 μm). .. For high resolution imagens slices were mounted in liquid antifade Abberior Mount Medium (Abberior, MM-2009-1ML) and 2D immunofluorescence images were acquired on an Abberior Stimulated Emission Depletion (STED) microscope equipped with a STEDYCON, equipped with 100x oil immersion objective (Nikon Plan Apo 100x/ 1.45 oil, w.d: 0.13 mm). ..



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    Image Search Results


    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

    Journal: bioRxiv

    Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth

    doi: 10.1101/2025.07.09.663961

    Figure Lengend Snippet: (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

    Article Snippet: For super resolution imaging, slides were imaged using a four detector Abberior STEDYCON STED unit.

    Techniques: FACS, Purification, Imaging, Isolation, Injection, Staining, Expressing, In Vitro, Transwell Migration Assay