Phospho-proteomics:Article Title: Oncostatin M Induces IFITM1 Expression to Inhibit Hepatitis B Virus Replication Via JAK-STAT Signaling
Article Snippet: The recombinant human cytokines IL6, IL11, IL31, IL35, LIF, OSM, CNTF, and cardiotrophin-1 were purchased from R&D Systems and Novoprotein (Shanghai, China). .. AG490 (STAT3 phosphorylation inhibitor), ruxolitinib (JAK1/2 phosphorylation inhibitor), and STAT5-IN (STAT5-DNA binding inhibitor) were manufactured by MedChemExpress (Shanghai, China). ..
Article Title: Oncostatin M Induces IFITM1 Expression to Inhibit Hepatitis B Virus Replication Via JAK-STAT Signaling.
Article Snippet: The recombinant human cytokines IL6, IL11, IL31, IL35, LIF, OSM, CNTF, and cardiotrophin-1 were purchased from R&D Systems and Novoprotein (Shanghai, China). .. AG490 (STAT3 phosphorylation inhibitor), ruxolitinib (JAK1/2 phosphorylation inhibitor), and STAT5-IN (STAT5-DNA binding inhibitor) were manufactured by MedChemExpress (Shanghai, China). .. Table 4.Antibodies Used for Western Blot and Immunostaining Antibody Company Cat# Host Dilution STAT1 Cell Signaling Technology #14994 Rabbit 1:1000 5 mL for IP phospho-STAT1 Cell Signaling Technology #9167 Rabbit 1:1000 STAT2 Cell Signaling Technology #72604 Rabbit 1:1000 phospho-STAT2 Cell Signaling Technology #88410 Rabbit 1:1000 STAT3 Cell Signaling Technology #12640 Rabbit 1:1000 phospho-STAT3 Cell Signaling Technology #9145 Rabbit 1:1000 STAT4 Affinity AF6441 Rabbit 1:1000 phospho-STAT4 Beyotime AF3441 Rabbit 1:1000 STAT5 Affinity AF6305 Rabbit 1:1000 phospho-STAT5 Beyotime AF2005 Rabbit 1:1000 STAT6 Beyotime AF1534 Rabbit 1:1000 phospho-STAT6 Beyotime AF5950 Rabbit 1:1000 IRF1 Cell Signaling Technology #8478 Rabbit 1:1000 IRF3 Cell Signaling Technology #11904 Rabbit 1:1000 IRF9 Cell Signaling Technology #76684 Rabbit 1:1000 IFITM1 Thermo Fisher Scientific PA5-27495 Rabbit 1:1000 b-actin Cell Signaling Technology #8457 Rabbit 1:1000 GAPDH Cell Signaling Technology #5174 Rabbit 1:1000 S100A9 Affinity DF7596 Rabbit 1:1000 IFITM2 Affinity DF8964 Rabbit 1:1000 LCN2 Affinity AF7362 Rabbit 1:1000 STING Beyotime AF8073 Rabbit 1:1000 HBsAg Novus Biologicals NB100-62652 Rabbit 1:1000 HBcAg Abcam ab8637 Mouse 1:1000 p38 Beyotime AF1111 Rabbit 1:1000 ERK1/2 Beyotime AF1051 Rabbit 1:1000 phospho-p38 Cell Signaling Technology #4511 Rabbit 1:1000 phospho-ERK1/2 Cell Signaling Technology #4370 Rabbit 1:1000 HNF4a Abcam ab92378 Rabbit 1:1000 HBx Abcam ab2741 Mouse 1:1000
Binding Assay:Article Title: Oncostatin M Induces IFITM1 Expression to Inhibit Hepatitis B Virus Replication Via JAK-STAT Signaling
Article Snippet: The recombinant human cytokines IL6, IL11, IL31, IL35, LIF, OSM, CNTF, and cardiotrophin-1 were purchased from R&D Systems and Novoprotein (Shanghai, China). .. AG490 (STAT3 phosphorylation inhibitor), ruxolitinib (JAK1/2 phosphorylation inhibitor), and STAT5-IN (STAT5-DNA binding inhibitor) were manufactured by MedChemExpress (Shanghai, China). ..
Article Title: Oncostatin M Induces IFITM1 Expression to Inhibit Hepatitis B Virus Replication Via JAK-STAT Signaling.
Article Snippet: The recombinant human cytokines IL6, IL11, IL31, IL35, LIF, OSM, CNTF, and cardiotrophin-1 were purchased from R&D Systems and Novoprotein (Shanghai, China). .. AG490 (STAT3 phosphorylation inhibitor), ruxolitinib (JAK1/2 phosphorylation inhibitor), and STAT5-IN (STAT5-DNA binding inhibitor) were manufactured by MedChemExpress (Shanghai, China). .. Table 4.Antibodies Used for Western Blot and Immunostaining Antibody Company Cat# Host Dilution STAT1 Cell Signaling Technology #14994 Rabbit 1:1000 5 mL for IP phospho-STAT1 Cell Signaling Technology #9167 Rabbit 1:1000 STAT2 Cell Signaling Technology #72604 Rabbit 1:1000 phospho-STAT2 Cell Signaling Technology #88410 Rabbit 1:1000 STAT3 Cell Signaling Technology #12640 Rabbit 1:1000 phospho-STAT3 Cell Signaling Technology #9145 Rabbit 1:1000 STAT4 Affinity AF6441 Rabbit 1:1000 phospho-STAT4 Beyotime AF3441 Rabbit 1:1000 STAT5 Affinity AF6305 Rabbit 1:1000 phospho-STAT5 Beyotime AF2005 Rabbit 1:1000 STAT6 Beyotime AF1534 Rabbit 1:1000 phospho-STAT6 Beyotime AF5950 Rabbit 1:1000 IRF1 Cell Signaling Technology #8478 Rabbit 1:1000 IRF3 Cell Signaling Technology #11904 Rabbit 1:1000 IRF9 Cell Signaling Technology #76684 Rabbit 1:1000 IFITM1 Thermo Fisher Scientific PA5-27495 Rabbit 1:1000 b-actin Cell Signaling Technology #8457 Rabbit 1:1000 GAPDH Cell Signaling Technology #5174 Rabbit 1:1000 S100A9 Affinity DF7596 Rabbit 1:1000 IFITM2 Affinity DF8964 Rabbit 1:1000 LCN2 Affinity AF7362 Rabbit 1:1000 STING Beyotime AF8073 Rabbit 1:1000 HBsAg Novus Biologicals NB100-62652 Rabbit 1:1000 HBcAg Abcam ab8637 Mouse 1:1000 p38 Beyotime AF1111 Rabbit 1:1000 ERK1/2 Beyotime AF1051 Rabbit 1:1000 phospho-p38 Cell Signaling Technology #4511 Rabbit 1:1000 phospho-ERK1/2 Cell Signaling Technology #4370 Rabbit 1:1000 HNF4a Abcam ab92378 Rabbit 1:1000 HBx Abcam ab2741 Mouse 1:1000
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