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antibodies against p stat3 tyr705  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antibodies against p stat3 tyr705
    Antibodies Against P Stat3 Tyr705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 6141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat3/Phospho-Stat3+(Tyr705)+XP+Rabbit+mAb/pm42888446-183-9-13
    Average 98 stars, based on 6141 article reviews
    antibodies against p stat3 tyr705 - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Reduced IL10 expression in diminished ovarian reserve and receptor-dependent IL-10 responses in granulosa cell models
    Article Snippet: .. After blocking with 5% BSA in TBST, Membranes were incubated overnight at 4°C with primary antibodies against phospho-STAT3 (Tyr705; CST; #9145), total STAT3 (CST; #9139), and either β-actin (CST; #4970) or GAPDH (Proteintech; HRP−60004), as indicated, with β-actin or GAPDH serving as the loading control, Membranes incubated with unconjugated primary antibodies were subsequently incubated with the appropriate HRP-conjugated secondary antibodies (CST; #7074). .. Bands were visualized using enhanced chemiluminescence reagents (Epizyme; SQ101) and imaged on a chemiluminescence detection system (e-BLOT Life Science, Touch imager).

    Article Title: Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts
    Article Snippet: .. Following blocking, the membranes were incubated overnight at 4°C with primary antibodies, including AIM2 (1:1000, catalog number ab93015, Abcam), Glutathione Peroxidase 4 (GPX4, 1:1000, 52455, Cell Signaling Technology, CST), solute carrier family 7 member 11 (SLC7A11, 1:1000, 23276, CST), phospho-STAT3 (p-STAT3, 1:1000, 9145, CST), STAT3 (1:1000, 12640, CST), pro-IL-1β (1:1000, 12703, CST), IL-1β (1:1000, ab283818, Abcam), cleaved caspase-1 (1:1000, 4199, CST), pro-caspase-1 (1:1000, 24232, CST), STAT1 (1:1000, 9172T, CST), phospho-STAT1 (p-STAT1, 1:1000, 9177S, CST), IRF1 (1:1000, 9172T, CST) and β-actin (1:1000, 4967S, CST). .. After three washes with TBST, the membranes were incubated with a secondary antibody (1:5000, ab288151, Abcam) for 2 hours.

    Incubation:

    Article Title: Reduced IL10 expression in diminished ovarian reserve and receptor-dependent IL-10 responses in granulosa cell models
    Article Snippet: .. After blocking with 5% BSA in TBST, Membranes were incubated overnight at 4°C with primary antibodies against phospho-STAT3 (Tyr705; CST; #9145), total STAT3 (CST; #9139), and either β-actin (CST; #4970) or GAPDH (Proteintech; HRP−60004), as indicated, with β-actin or GAPDH serving as the loading control, Membranes incubated with unconjugated primary antibodies were subsequently incubated with the appropriate HRP-conjugated secondary antibodies (CST; #7074). .. Bands were visualized using enhanced chemiluminescence reagents (Epizyme; SQ101) and imaged on a chemiluminescence detection system (e-BLOT Life Science, Touch imager).

    Article Title: Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts
    Article Snippet: .. Following blocking, the membranes were incubated overnight at 4°C with primary antibodies, including AIM2 (1:1000, catalog number ab93015, Abcam), Glutathione Peroxidase 4 (GPX4, 1:1000, 52455, Cell Signaling Technology, CST), solute carrier family 7 member 11 (SLC7A11, 1:1000, 23276, CST), phospho-STAT3 (p-STAT3, 1:1000, 9145, CST), STAT3 (1:1000, 12640, CST), pro-IL-1β (1:1000, 12703, CST), IL-1β (1:1000, ab283818, Abcam), cleaved caspase-1 (1:1000, 4199, CST), pro-caspase-1 (1:1000, 24232, CST), STAT1 (1:1000, 9172T, CST), phospho-STAT1 (p-STAT1, 1:1000, 9177S, CST), IRF1 (1:1000, 9172T, CST) and β-actin (1:1000, 4967S, CST). .. After three washes with TBST, the membranes were incubated with a secondary antibody (1:5000, ab288151, Abcam) for 2 hours.

    Article Title: KLF4 Protects Against Sepsis-Induced Coagulopathy via Inhibition of the AKT/mTOR/STAT3 Signaling Pathway
    Article Snippet: .. The PVDF membranes were blocked with 5% skimmed milk for 2 h at room temperature, followed by incubation with thrombin (ab314480, 1:1000, Abcam), PAI-1 (ab222754, 1:1000, Abcam), TF (DF6400, 1:2000, affinity), GAPDH (AF7021, 1:5000, affinity), Tubulin (AF7011,1:1000, affinity), p-AKT (4060S, 1:2000, CST), AKT (4691S, 1:1000, CST), p-mTOR (5536S, 1:2000, CST), mTOR (2983S, 1:1000, CST), KLF4 (4038T, 1:1000, CST), p-STAT3 (9145T, 1:2000, CST) and STAT3 (9139T, 1:1000, CST) were incubated at 4°C overnight. .. The next day, the membranes were incubated with HRPlabeled affinity-pure goat anti-rabbit IgG (H+L) (SA00001-2, 1:15000, Proteintech) or HRP-labeled affinity-pure goat anti-mouse IgG (H+L) (SA00001-1, 1:15000, Proteintech) for 1 h at room temperature.

    Article Title: Qingjin Pingchuan formula attenuates pulmonary inflammation by reprogramming neutrophil SHP1-JAK2/SRC-STAT3 signaling.
    Article Snippet: Background: The Qingjin Pingchuan Formula (QJPC), a multi-herb traditional Chinese medicine prescription, is clinically employed for severe community-acquired pneumonia (SCAP), though its underlying molecular mechanisms remain incompletely understood.. Purpose: This study aimed to elucidate the anti-inflammatory effects of QJPC and its impact on neutrophilmediated signaling pathways.. Methods: An integrative strategy combining network pharmacology, single-cell RNA sequencing (scRNA-seq), and experimental validation was applied.

    Control:

    Article Title: Reduced IL10 expression in diminished ovarian reserve and receptor-dependent IL-10 responses in granulosa cell models
    Article Snippet: .. After blocking with 5% BSA in TBST, Membranes were incubated overnight at 4°C with primary antibodies against phospho-STAT3 (Tyr705; CST; #9145), total STAT3 (CST; #9139), and either β-actin (CST; #4970) or GAPDH (Proteintech; HRP−60004), as indicated, with β-actin or GAPDH serving as the loading control, Membranes incubated with unconjugated primary antibodies were subsequently incubated with the appropriate HRP-conjugated secondary antibodies (CST; #7074). .. Bands were visualized using enhanced chemiluminescence reagents (Epizyme; SQ101) and imaged on a chemiluminescence detection system (e-BLOT Life Science, Touch imager).

    Article Title: Targeting the C/EBPβ-PRAME-EZH2 complex modulates the Netrin-4/AKT axis to inhibit renal cancer tumorigenesis and metastasis.
    Article Snippet: Cancer-testis antigens are considered clinically attractive targets for cancer treatment, but their functions and mechanisms are not well elucidated.. Here, based on comprehensive bioinformatics analyses, we identify PRAME, a nuclear cancer-testis antigen, as a potential regulator of metastasis in clear cell renal cell carcinoma (ccRCC).. Subsequent RNA-Seq and functional studies illustrate that Netrin-4 (NTN4) is a major downstream effector of PRAME, involved in its oncogenic functions.

    Cell-Signaling:

    Article Title: Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts
    Article Snippet: .. Following blocking, the membranes were incubated overnight at 4°C with primary antibodies, including AIM2 (1:1000, catalog number ab93015, Abcam), Glutathione Peroxidase 4 (GPX4, 1:1000, 52455, Cell Signaling Technology, CST), solute carrier family 7 member 11 (SLC7A11, 1:1000, 23276, CST), phospho-STAT3 (p-STAT3, 1:1000, 9145, CST), STAT3 (1:1000, 12640, CST), pro-IL-1β (1:1000, 12703, CST), IL-1β (1:1000, ab283818, Abcam), cleaved caspase-1 (1:1000, 4199, CST), pro-caspase-1 (1:1000, 24232, CST), STAT1 (1:1000, 9172T, CST), phospho-STAT1 (p-STAT1, 1:1000, 9177S, CST), IRF1 (1:1000, 9172T, CST) and β-actin (1:1000, 4967S, CST). .. After three washes with TBST, the membranes were incubated with a secondary antibody (1:5000, ab288151, Abcam) for 2 hours.

    Article Title: Targeting the C/EBPβ-PRAME-EZH2 complex modulates the Netrin-4/AKT axis to inhibit renal cancer tumorigenesis and metastasis.
    Article Snippet: Cancer-testis antigens are considered clinically attractive targets for cancer treatment, but their functions and mechanisms are not well elucidated.. Here, based on comprehensive bioinformatics analyses, we identify PRAME, a nuclear cancer-testis antigen, as a potential regulator of metastasis in clear cell renal cell carcinoma (ccRCC).. Subsequent RNA-Seq and functional studies illustrate that Netrin-4 (NTN4) is a major downstream effector of PRAME, involved in its oncogenic functions.

    Western Blot:

    Article Title: Sirtuin 1 Inhibition Attenuates Viral Myocarditis by Suppressing the Nuclear Translocation of Pyruvate Kinase M2 to Rebalance Th17/Th9 Differentiation
    Article Snippet: .. The Western blotting antibodies used targeted following proteins: SIRT1 (1:1000, catalog: 2028, CST, USA), PKM2 (1:1000, catalog:15822-1-AP, Proteintech, China), ALDOA (1:5000, catalog: 11217-1-AP, Proteintech, China), HK2 (1:5000, catalog: 22029-1-AP, Proteintech, China), PFKFB1 (1:1000, catalog: 21718-1-AP, Proteintech, China), GLUT1 (1:2000, catalog: A11208, Abclonal, China), STAT3 (1:1000, catalog: 12640, CST, USA), phospho-STAT3 (1:1000, catalog: 9145, CST, USA), STAT5 (1:1000, catalog: 94205, CST, USA), phospho-STAT5 (1:1000, catalog: 9359, CST, USA), RORγt (1:1000, catalog:ab207082, abcam, UK), PU.1 (1:1000, catalog: 2258, CST, USA), Foxp3 (1:500, catalog:85128-6-RR, Proteintech, China), β-actin (1:3000, catalog: ANT321, Antgene, China), GAPDH (1:5000, catalog: 60004-1-Ig, Proteintech, China), and H3 (1:2000, catalog:17168-1-AP, Proteintech, China). .. Following primary antibody incubation, the membranes were probed with respective anti-rabbit or antimouse HRP-conjugated secondary antibodies (1:3000, AntGene, China) for 2 hours at room temperature and visualized using the electrochemiluminescence (ECL) kit (Thermo Scientific, USA).

    Sequencing:

    Article Title: Knockdown of CCT2 inhibits the malignant progression of hepatocellular carcinoma cells by impairing STAT3 activation
    Article Snippet: .. The primary antibodies were as follows: CCT2 (cat. no. 24896-1-AP), β-actin (cat. no. 66009-1-Ig), MMP2 (cat. no. 10373-2-AP), myeloid cell leukemia sequence 1 (MCL1; cat. no. 16225-1-AP) and SRY-box transcription factor 2 (SOX2; cat. no. 11064-1-AP; all Proteintech Group, Inc.) and STAT3 (cat. no. 4904) and phosphorylated (p-)STAT3 (Tyr705; cat. no. 4113; both Cell Signaling Technology, Inc.) The membranes were washed three times in TBST (0.1% Tween-20) for 5 min each at room temperature. ..

    Hemagglutination Assay:

    Article Title: Targeting the C/EBPβ-PRAME-EZH2 complex modulates the Netrin-4/AKT axis to inhibit renal cancer tumorigenesis and metastasis.
    Article Snippet: Cancer-testis antigens are considered clinically attractive targets for cancer treatment, but their functions and mechanisms are not well elucidated.. Here, based on comprehensive bioinformatics analyses, we identify PRAME, a nuclear cancer-testis antigen, as a potential regulator of metastasis in clear cell renal cell carcinoma (ccRCC).. Subsequent RNA-Seq and functional studies illustrate that Netrin-4 (NTN4) is a major downstream effector of PRAME, involved in its oncogenic functions.

    other:

    Article Title: Inhibition of CDK9 sensitizes multidrug resistant ovarian cancer cells to paclitaxel
    Article Snippet: The Stat3 pathway associated antibodies, including Stat3 and phosphorylated Stat3 (Tyr705) were also purchased from Cell Signaling Technology.



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