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cfx96 real time pcr detection kit  (Bio-Rad)


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    Bio-Rad cfx96 real time pcr detection kit
    Cfx96 Real Time Pcr Detection Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 26016 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfx96 real time pcr detection kit/product/Bio-Rad
    Average 96 stars, based on 26016 article reviews
    cfx96 real time pcr detection kit - by Bioz Stars, 2026-05
    96/100 stars

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    Image Search Results


    Cell selection using automated magnetic cell sorting (A) Cells were stained with CD45-biotin and CD31-biotin and sorted using autoMACS® Pro Separator. (B) Number of cells before staining for autoMACS® separation (step 11), and number of cells recovered from positive selection (CD45 + and CD31 + cells) and negative selection (CD45 - and CD31 - cells) in step 23. Each dot represents combined numbers from inguinal, axillary and brachial lymph nodes from 6 mice. Colors indicate biological replicates. (C) Percentages of cells after separation compared to the pre-staining cell count performed in step 11. (D) Purity check of separated cells using flow cytometry and staining for CD45 and CD31. (E) Overlay plots of positive selection (blue) and negative selection (red). (F) Percentage of CD45 + , CD45 - and CD31 + cells recovered (∗∗∗∗ p value < 0.0001, ∗ p value < 0.05). (G) Final viability check of positive and negative selected cells acquired just before performing scRNA-sequencing analysis.

    Journal: STAR Protocols

    Article Title: Protocol for isolating stromal cells from lymphoid tissue for performing scRNA-seq

    doi: 10.1016/j.xpro.2026.104501

    Figure Lengend Snippet: Cell selection using automated magnetic cell sorting (A) Cells were stained with CD45-biotin and CD31-biotin and sorted using autoMACS® Pro Separator. (B) Number of cells before staining for autoMACS® separation (step 11), and number of cells recovered from positive selection (CD45 + and CD31 + cells) and negative selection (CD45 - and CD31 - cells) in step 23. Each dot represents combined numbers from inguinal, axillary and brachial lymph nodes from 6 mice. Colors indicate biological replicates. (C) Percentages of cells after separation compared to the pre-staining cell count performed in step 11. (D) Purity check of separated cells using flow cytometry and staining for CD45 and CD31. (E) Overlay plots of positive selection (blue) and negative selection (red). (F) Percentage of CD45 + , CD45 - and CD31 + cells recovered (∗∗∗∗ p value < 0.0001, ∗ p value < 0.05). (G) Final viability check of positive and negative selected cells acquired just before performing scRNA-sequencing analysis.

    Article Snippet: autoMACS® Pro Separator , Miltenyi Biotec , Cat# 130-092-545.

    Techniques: Selection, FACS, Staining, Cell Characterization, Flow Cytometry, Sequencing