Journal: Journal of Orthopaedic Translation

Article Title: Extracellular vesicles originating from induced pluripotent stem cell-derived chondrocytes facilitate the regeneration of osteoarthritic cartilage

doi: 10.1016/j.jot.2025.101035

Figure Lengend Snippet: iChondrocyte-sEVs-derived SRR facilitated cartilage regeneration via the P38/ERK pathway. a Representative histopathological image of SRR in articular cartilage (n = 6). Scale bar: 200 μm b Western Blot analysis of SRR levels in iPSC-sEVs, iMSC-sEVs and iChondrocyte-sEVs (n = 3). c Western Blot analysis of SRR, SOX9, COLII expression in MSC-derived chondrocytes treated with or without iChondrocyte-sEVs (n = 3). d Western Blot analysis of key proteins in the P38/ERK signaling pathway in MSC-derived chondrocytes treated with or without iChondrocyte-sEVs (n = 3). e Schematic diagram illustrating the molecular mechanism by which iChondrocyte-sEVs exert their regenerative effects. All groups except the sham group were conducted under DMM conditions.

Article Snippet: The following primary antibodies were used for western blot analysis: CD9 (RGAB101, Rengen Biosciences, China), CD63 (RGAB103, Rengen Biosciences, China), Alix (RGAB100, Rengen Biosciences, China), SRR (17955-1-AP, Proteintech, China), SOX9 (GB11280, Servicebio, China), COLII (28459, Proteintech, China), ACAN (68350, Proteintech, China), β-actin (GB15003, Servicebio, China), SynGAP (19739, Proteintech, China), JNK1 ( A23206 , Abclonal, China), P-JNK (AP1337, Abclonal, China), P38 ( GB154685 , Servicebio, China), P-P38 ( GB153380 , Servicebio, China), ERK (4695, CST, USA), and P-ERK(4370, CST, USA).

Techniques: Derivative Assay, Western Blot, Expressing

Journal: Bioactive Materials

Article Title: Modulation of bone homeostasis by dual drug-loaded premixed magnesium tri-magnesium phosphate bone cement for the treatment of osteoporotic vertebral compression fractures

doi: 10.1016/j.bioactmat.2025.08.022

Figure Lengend Snippet: Schematic illustration of constructing ready-to-use premixed bone cement. Bioglass spheres (BG) loaded with alendronate sodium (AS), strontium ranelate (SrR), and trimagnesium phosphate bone cement powder were mixed with glyceryl tributyrate citrate (GTCC) as a dispersing agent to obtain pTMPC-SMA bone cement. pTMPC-SMA could inhibited the osteoclast differential through TNF and OPG/RANK/RANKL pathways. The release of alendronate sodium directly inhibited osteoclast activity. Strontium ranelate could promoted osteoblast differentiation and enhanced OPG expression, thereby blocking the binding of RANKL to RANK and reducing osteoclast differentiation. Percutaneous vertebroplasty (PVP) surgery was conducted successfully on osteoporosis rabbits.After the cement degradation, new bone grew into the cement and enhanced the strength of the vertebral body.

Article Snippet: MgHPO 4 ⋅3H 2 O, Mg(OH) 2 , strontium ranelate (SrR), alendronate sodium (AS), polyvinylpyrrolidone K-60, 9-fluorenylmethyl chloroformate (FMOC), hexadecyl trimethyl ammonium bromide (CTAB), sodium hydroxide and tetraethyl orthosilicate (TEOS) were purchased from Macklin, China.

Techniques: Activity Assay, Expressing, Blocking Assay, Binding Assay