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Assembly statistics, genomic features and predicted number of insertion sequence (IS) elements of the three S. sonnei isolates and one S. flexneri 3a isolate. Genomes were constructed with Flye, where <t> Illumina </t> short reads were utilised to polish a draft long-read assembly to yield a more complete and accurate final assembly
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Assembly statistics, genomic features and predicted number of insertion sequence (IS) elements of the three S. sonnei isolates and one S. flexneri 3a isolate. Genomes were constructed with Flye, where <t> Illumina </t> short reads were utilised to polish a draft long-read assembly to yield a more complete and accurate final assembly
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Assembly statistics, genomic features and predicted number of insertion sequence (IS) elements of the three S. sonnei isolates and one S. flexneri 3a isolate. Genomes were constructed with Flye, where <t> Illumina </t> short reads were utilised to polish a draft long-read assembly to yield a more complete and accurate final assembly
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Image Search Results


Assembly statistics, genomic features and predicted number of insertion sequence (IS) elements of the three S. sonnei isolates and one S. flexneri 3a isolate. Genomes were constructed with Flye, where  Illumina  short reads were utilised to polish a draft long-read assembly to yield a more complete and accurate final assembly

Journal: Microbial Genomics

Article Title: Acquisition and loss of CTX-M plasmids in Shigella species associated with MSM transmission in the UK

doi: 10.1099/mgen.0.000644

Figure Lengend Snippet: Assembly statistics, genomic features and predicted number of insertion sequence (IS) elements of the three S. sonnei isolates and one S. flexneri 3a isolate. Genomes were constructed with Flye, where Illumina short reads were utilised to polish a draft long-read assembly to yield a more complete and accurate final assembly

Article Snippet: SRR number (Illumina ) , SRR7842065 , SRR7892120 , SRR11096691 , SRR11206407.

Techniques: Sequencing, Construct, Plasmid Preparation

Maximum-likelihood phylogeny of S. flexneri 3a isolate 888 048 (SRR11096691, indicated by a filled orange arrow) sequenced with Nanopore technologies in this study, compared to other S. flexneri isolates in Public Health England’s collections. To place the isolate in context, 49 other S. flexneri isolates that are representatives from relevant SNP (single nucleotide polymorphism) clusters were included in the comparison. MSM clades associated with S. flexneri 1b, S. flexneri 2a and S. flexneri 3a are labelled. Isolates are labelled by SRR number and tip nodes are coloured by year of receipt. Adjacent grey blocks in each of the first three columns indicate the sample was isolated from a male, an adult, and that the illness was non-travel related (left-right). Grey blocks in these three lanes for an isolate implies the infection may be associated with MSM transmission. Presence of antimicrobial resistance genes concerning fluoroquinolones (mutations in gyrA and parC , green) and macrolides ( erm(B ) and mph(A), orange) are taken from Bardsley et al . and presence of bla CTX-M-27 was determined using GeneFinder. Gene presence is indicated by a coloured tile in the relevant column. ML trees are midpoint rooted and scale bar indicates SNPs.

Journal: Microbial Genomics

Article Title: Acquisition and loss of CTX-M plasmids in Shigella species associated with MSM transmission in the UK

doi: 10.1099/mgen.0.000644

Figure Lengend Snippet: Maximum-likelihood phylogeny of S. flexneri 3a isolate 888 048 (SRR11096691, indicated by a filled orange arrow) sequenced with Nanopore technologies in this study, compared to other S. flexneri isolates in Public Health England’s collections. To place the isolate in context, 49 other S. flexneri isolates that are representatives from relevant SNP (single nucleotide polymorphism) clusters were included in the comparison. MSM clades associated with S. flexneri 1b, S. flexneri 2a and S. flexneri 3a are labelled. Isolates are labelled by SRR number and tip nodes are coloured by year of receipt. Adjacent grey blocks in each of the first three columns indicate the sample was isolated from a male, an adult, and that the illness was non-travel related (left-right). Grey blocks in these three lanes for an isolate implies the infection may be associated with MSM transmission. Presence of antimicrobial resistance genes concerning fluoroquinolones (mutations in gyrA and parC , green) and macrolides ( erm(B ) and mph(A), orange) are taken from Bardsley et al . and presence of bla CTX-M-27 was determined using GeneFinder. Gene presence is indicated by a coloured tile in the relevant column. ML trees are midpoint rooted and scale bar indicates SNPs.

Article Snippet: SRR number (Illumina ) , SRR7842065 , SRR7892120 , SRR11096691 , SRR11206407.

Techniques: Comparison, Isolation, Infection, Transmission Assay

Maximum-likelihood phylogeny of three S. sonnei isolates known to be associated with men who have sex with men that were sequenced using Nanopore technologies in this study, compared to other isolates in Public Health England’s collections. To place the isolates in context, 198 other S. flexneri isolates that are representatives from relevant SNP (single nucleotide polymorphism) clusters were included in the comparison. S. sonnei isolates investigated in detail in this study are as follows; 598 080 (SRR7842065, pink filled arrow), 607 387 (SRR7892120, green filled arrow), 893 916 (SRR11206407, blue filled arrow). Isolates are labelled by SRR number and tip nodes are coloured by year of receipt. S. sonnei MSM clades are labelled as per those described in Baker et al . . Adjacent grey blocks in each of the first three columns (left-right) indicate the sample was isolated from a male, an adult, and that the illness was non-travel related (left-right). Grey blocks in these three lanes for an isolate implies the infection may be associated with MSM transmission. Presence of antimicrobial resistance genes concerning fluoroquinolones (mutations in gyrA and parC , green) and macrolides ( erm(B ) and mph(A), orange) are taken from Bardsley et al. and presence of bla CTX-M-27 was determined using GeneFinder. Gene presence is indicated by a coloured tile in the relevant column. ML trees are midpoint rooted and scale bar indicates SNPs.

Journal: Microbial Genomics

Article Title: Acquisition and loss of CTX-M plasmids in Shigella species associated with MSM transmission in the UK

doi: 10.1099/mgen.0.000644

Figure Lengend Snippet: Maximum-likelihood phylogeny of three S. sonnei isolates known to be associated with men who have sex with men that were sequenced using Nanopore technologies in this study, compared to other isolates in Public Health England’s collections. To place the isolates in context, 198 other S. flexneri isolates that are representatives from relevant SNP (single nucleotide polymorphism) clusters were included in the comparison. S. sonnei isolates investigated in detail in this study are as follows; 598 080 (SRR7842065, pink filled arrow), 607 387 (SRR7892120, green filled arrow), 893 916 (SRR11206407, blue filled arrow). Isolates are labelled by SRR number and tip nodes are coloured by year of receipt. S. sonnei MSM clades are labelled as per those described in Baker et al . . Adjacent grey blocks in each of the first three columns (left-right) indicate the sample was isolated from a male, an adult, and that the illness was non-travel related (left-right). Grey blocks in these three lanes for an isolate implies the infection may be associated with MSM transmission. Presence of antimicrobial resistance genes concerning fluoroquinolones (mutations in gyrA and parC , green) and macrolides ( erm(B ) and mph(A), orange) are taken from Bardsley et al. and presence of bla CTX-M-27 was determined using GeneFinder. Gene presence is indicated by a coloured tile in the relevant column. ML trees are midpoint rooted and scale bar indicates SNPs.

Article Snippet: SRR number (Illumina ) , SRR7842065 , SRR7892120 , SRR11096691 , SRR11206407.

Techniques: Comparison, Isolation, Infection, Transmission Assay