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split components  (New England Biolabs)


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    New England Biolabs split components
    Split Components, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/split+components/NEBuffer+4/pm41944382-284-31-24
    Average 98 stars, based on 1093 article reviews
    split components - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: SDS-CRISPR for Single-Nucleotide Variant Detection.
    Article Snippet: .. .14 SDS-CRISPR Assays for HIV Mutation etection he SDS-CRISPR trans -cleavage reaction was performed in a final olume of 10 μL containing 1 × NEBuffer 4, 200 nM crRNA full-sized or split components), 100 nM Alt-R Cas12a, 5 μM eporter, and 1 μL of 10 nM synthetic HIV dsDNA. ..



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    Image Search Results


    (A) The Gal4 transcription factor binds to UAS sequences to drive transcription, and can be repressed by the binding of Gal80. Gal4 is drawn here as a monomer, but functions as a dimer in vivo. (B) In the original split-Gal4 system, the Gal4DBD and a strong transcriptional activator (VP16 or p65) are each driven by separate enhancers, and reconstituted in cells by leucine zipper domains. Gal80 cannot bind or repress the split-Gal4 complex. (C) In the split-intein Gal4 system, two fragments of the Gal4 protein, each flanked by a split-intein, are independently driven by separate enhancers, and seamlessly trans-spliced to reconstitute a functional, wildtype Gal4 protein, which can be repressed by Gal80.

    Journal: bioRxiv

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80

    doi: 10.1101/2023.03.24.534001

    Figure Lengend Snippet: (A) The Gal4 transcription factor binds to UAS sequences to drive transcription, and can be repressed by the binding of Gal80. Gal4 is drawn here as a monomer, but functions as a dimer in vivo. (B) In the original split-Gal4 system, the Gal4DBD and a strong transcriptional activator (VP16 or p65) are each driven by separate enhancers, and reconstituted in cells by leucine zipper domains. Gal80 cannot bind or repress the split-Gal4 complex. (C) In the split-intein Gal4 system, two fragments of the Gal4 protein, each flanked by a split-intein, are independently driven by separate enhancers, and seamlessly trans-spliced to reconstitute a functional, wildtype Gal4 protein, which can be repressed by Gal80.

    Article Snippet: The inserts for the original split-Gal4 components (pAW-Zip − -Gal4DBD and pAW-p65-Zip + ) were amplified from pBPZpGAL4DBDUw (Addgene 26233) and pBPp65ADZpUw (Addgene 26234), respectively. pCaSpeR-tub-Gal80 was used to test for Gal80 sensitivity in S2R+ cells.

    Techniques: Binding Assay, In Vivo, Functional Assay

    (A) Cartoon schematic of the split-intein GeneSwitch system, not drawn to scale. The same N-terminal fragment of Gal4 used in split-intein Gal4 can be combined with the C-terminus of the GeneSwitch system, which includes amino acids 21-93 of Gal4, a progesterone ligand binding domain (PR-LBD) and the p65 transcriptional activator. (B) Drug-inducible ISC tumor model using the esg -Gal4 N-int ∩ tub- GeneSwitch C-int > UAS: yki S3A . Anterior is up. Scale bar = 50 µm.

    Journal: bioRxiv

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80

    doi: 10.1101/2023.03.24.534001

    Figure Lengend Snippet: (A) Cartoon schematic of the split-intein GeneSwitch system, not drawn to scale. The same N-terminal fragment of Gal4 used in split-intein Gal4 can be combined with the C-terminus of the GeneSwitch system, which includes amino acids 21-93 of Gal4, a progesterone ligand binding domain (PR-LBD) and the p65 transcriptional activator. (B) Drug-inducible ISC tumor model using the esg -Gal4 N-int ∩ tub- GeneSwitch C-int > UAS: yki S3A . Anterior is up. Scale bar = 50 µm.

    Article Snippet: The inserts for the original split-Gal4 components (pAW-Zip − -Gal4DBD and pAW-p65-Zip + ) were amplified from pBPZpGAL4DBDUw (Addgene 26233) and pBPp65ADZpUw (Addgene 26234), respectively. pCaSpeR-tub-Gal80 was used to test for Gal80 sensitivity in S2R+ cells.

    Techniques: Ligand Binding Assay