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split nanoluc component 11s  (Danaher Inc)


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    Danaher Inc split nanoluc component 11s
    Split Nanoluc Component 11s, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/split+components/pm38133598-189-11-18
    Average 86 stars, based on 1 article reviews
    split nanoluc component 11s - by Bioz Stars, 2026-10
    86/100 stars

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    Article Title: Design of Ligand-Operable Protein-Cages That Open Upon Specific Protein Binding.
    Article Snippet: Protein nanocages have diverse applications in medicine and biotechnology, including molecular delivery.. However, although numerous studies have demonstrated the ability of protein nanocages to encapsulate various molecular species, limited methods are available for subsequently opening a nanocage for cargo release under specific conditions.. A modular platform with a specific protein-target-based mechanism of nanocage opening is notably lacking.



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    (A) The Gal4 transcription factor binds to UAS sequences to drive transcription, and can be repressed by the binding of Gal80. Gal4 is drawn here as a monomer, but functions as a dimer in vivo. (B) In the original split-Gal4 system, the Gal4DBD and a strong transcriptional activator (VP16 or p65) are each driven by separate enhancers, and reconstituted in cells by leucine zipper domains. Gal80 cannot bind or repress the split-Gal4 complex. (C) In the split-intein Gal4 system, two fragments of the Gal4 protein, each flanked by a split-intein, are independently driven by separate enhancers, and seamlessly trans-spliced to reconstitute a functional, wildtype Gal4 protein, which can be repressed by Gal80.

    Journal: bioRxiv

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80

    doi: 10.1101/2023.03.24.534001

    Figure Lengend Snippet: (A) The Gal4 transcription factor binds to UAS sequences to drive transcription, and can be repressed by the binding of Gal80. Gal4 is drawn here as a monomer, but functions as a dimer in vivo. (B) In the original split-Gal4 system, the Gal4DBD and a strong transcriptional activator (VP16 or p65) are each driven by separate enhancers, and reconstituted in cells by leucine zipper domains. Gal80 cannot bind or repress the split-Gal4 complex. (C) In the split-intein Gal4 system, two fragments of the Gal4 protein, each flanked by a split-intein, are independently driven by separate enhancers, and seamlessly trans-spliced to reconstitute a functional, wildtype Gal4 protein, which can be repressed by Gal80.

    Article Snippet: The inserts for the original split-Gal4 components (pAW-Zip − -Gal4DBD and pAW-p65-Zip + ) were amplified from pBPZpGAL4DBDUw (Addgene 26233) and pBPp65ADZpUw (Addgene 26234), respectively. pCaSpeR-tub-Gal80 was used to test for Gal80 sensitivity in S2R+ cells.

    Techniques: Binding Assay, In Vivo, Functional Assay

    (A) Cartoon schematic of the split-intein GeneSwitch system, not drawn to scale. The same N-terminal fragment of Gal4 used in split-intein Gal4 can be combined with the C-terminus of the GeneSwitch system, which includes amino acids 21-93 of Gal4, a progesterone ligand binding domain (PR-LBD) and the p65 transcriptional activator. (B) Drug-inducible ISC tumor model using the esg -Gal4 N-int ∩ tub- GeneSwitch C-int > UAS: yki S3A . Anterior is up. Scale bar = 50 µm.

    Journal: bioRxiv

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80

    doi: 10.1101/2023.03.24.534001

    Figure Lengend Snippet: (A) Cartoon schematic of the split-intein GeneSwitch system, not drawn to scale. The same N-terminal fragment of Gal4 used in split-intein Gal4 can be combined with the C-terminus of the GeneSwitch system, which includes amino acids 21-93 of Gal4, a progesterone ligand binding domain (PR-LBD) and the p65 transcriptional activator. (B) Drug-inducible ISC tumor model using the esg -Gal4 N-int ∩ tub- GeneSwitch C-int > UAS: yki S3A . Anterior is up. Scale bar = 50 µm.

    Article Snippet: The inserts for the original split-Gal4 components (pAW-Zip − -Gal4DBD and pAW-p65-Zip + ) were amplified from pBPZpGAL4DBDUw (Addgene 26233) and pBPp65ADZpUw (Addgene 26234), respectively. pCaSpeR-tub-Gal80 was used to test for Gal80 sensitivity in S2R+ cells.

    Techniques: Ligand Binding Assay