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MicroSource Discovery Systems spectrum collection
Spectrum Collection, supplied by MicroSource Discovery Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spectrum+collection/spectrum+collection/10__1016_slash_j__jpet__2025__103660-65-3-5
Average 90 stars, based on 1 article reviews
spectrum collection - by Bioz Stars, 2026-10
90/100 stars

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Clinical Proteomics:

Article Title: Administration of novobiocin and apomorphine mitigates cholera toxin mediated cellular toxicity: Lessons from cholera toxin yeast model system.
Article Snippet: .. The library “Spectrum collection” from Microsource Discovery systems Inc. https://doi.org/10.1371/journal.pone.0315052.g003 PLOS ONE | https://doi.org/10.1371/journal.pone.0315052 December 5, 2024 7 / 14 used in our study is also exploited by other groups extensively for the discovery of many novel and repurposing drug molecules some of which are now under clinical trials [48]. ..

Article Title: Administration of novobiocin and apomorphine mitigates cholera toxin mediated cellular toxicity: Lessons from cholera toxin yeast model system
Article Snippet: .. The library “Spectrum collection” from Microsource Discovery systems Inc. used in our study is also exploited by other groups extensively for the discovery of many novel and repurposing drug molecules some of which are now under clinical trials [ ]. ..

Food & Beverages:

Article Title: Autophagy induction by piplartine ameliorates axonal degeneration caused by mutant HSPB1 and HSPB8 in Charcot-Marie-Tooth type 2 neuropathies
Article Snippet: All these last steps were performed with BioTek EL406 Washer Dispenser (BioTek Instruments, Agilent, CA). .. In the primary screening, the Spectrum Collection (Microsource Discovery Systems), which includes 2050 Food and Drug Administration/European Medicine agency (FDA/EMA)-approved drugs, natural compounds, and molecules in preclinical stages, was tested on Hspb8 K141N/K141N MEFs, as described in the pilot screening. ..

Article Title: Autophagy induction by piplartine ameliorates axonal degeneration caused by mutant HSPB1 and HSPB8 in Charcot-Marie-Tooth type 2 neuropathies.
Article Snippet: All these last steps were performed with BioTek EL406 Washer Dispenser (BioTek Instruments, Agilent, CA). .. Primary screening and dose-response curve In the primary screening, the Spectrum Collection (Microsource Discovery Systems), which includes 2050 Food and Drug Administration/European Medicine agency (FDA/EMA)approved drugs, natural compounds, and molecules in preclinical stages, was tested on Hspb8K141N/K141N MEFs, as described in the pilot screening. ..

Concentration Assay:

Article Title: A rapid in vivo pipeline to identify small molecule inhibitors of amyloid aggregation
Article Snippet: Sample morphology was visualized using a JEM-1400 TEM (JEOL, Tokyo, Japan) accelerating at 80 kV . .. To identify chemical suppressors of crystal-induced death, the Spectrum Collection (MicroSource Discovery Systems Inc.) of 2560 approved drugs and natural products was screened at a concentration of 60 μM in the background of 3.75 μM wact-190. ..

High Throughput Screening Assay:

Article Title: Experimental and Computational Approaches for Evaluating Molecule Interactions with Equilibrative Nucleoside Transporters 1 and 2
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Assay Development:

Article Title: Experimental and Computational Approaches for Evaluating Molecule Interactions with Equilibrative Nucleoside Transporters 1 and 2
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Drug discovery:

Article Title: Experimental and Computational Approaches for Evaluating Molecule Interactions with Equilibrative Nucleoside Transporters 1 and 2
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Expressing:

Article Title: Experimental and Computational Approaches for Evaluating Molecule Interactions with Equilibrative Nucleoside Transporters 1 and 2
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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MicroSource Discovery Systems spectrum collection small molecule library
A. Schematic of high-through put screening: Pre-induced cultures of BY4741/ ctxA /pGML10 and BY4741/pGML10 as a control were used. These cultures were evenly distributed in 96 well plates, then compounds from the <t>library</t> plate were added at the final concentrations of 20 μM. Both plates were incubated for 24 h at 30°C and were then spotted on glucose and galactose plates for readouts. B. Schematic representation of the outcome of the <t>small</t> <t>molecule</t> library screening. Structures of the few positive hits from secondary screen are shown. C. Tertiary screening of Apomorphine HCl: Pre-induced cultures of BY4741/ ctxA /pGML10 and BY4741/pGML10 as a control were spotted on solid agar plate containing galactose and galactose with apomophine (50 μM). Pictures were taken after 60–70 h D and E. Effect of Apomorphine HCl on cholera toxicity in yeast: Secondary cultures of BY4741/ ctxA -HO and vector control were set up at starting OD 600 0.05 in glucose and galactose media with different concentrations of apomorphine and were monitored for 27 hrs. OD 600 was checked every 3 h. The data shown here is collected from 3 biological triplicates (D). At the end point of liquid growth assay, the cultures were serially diluted (10 0 , 10 2 , 10 3 , 10 4 ) and were spotted on glucose and galactose plates. Pictures were taken after 60–70 hrs (E). Liquid growth assay.
Spectrum Collection Small Molecule Library, supplied by MicroSource Discovery Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spectrum+collection/spectrum+collection+compound+library/pmc11620602-52-0-5
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A. Schematic of high-through put screening: Pre-induced cultures of BY4741/ ctxA /pGML10 and BY4741/pGML10 as a control were used. These cultures were evenly distributed in 96 well plates, then compounds from the library plate were added at the final concentrations of 20 μM. Both plates were incubated for 24 h at 30°C and were then spotted on glucose and galactose plates for readouts. B. Schematic representation of the outcome of the small molecule library screening. Structures of the few positive hits from secondary screen are shown. C. Tertiary screening of Apomorphine HCl: Pre-induced cultures of BY4741/ ctxA /pGML10 and BY4741/pGML10 as a control were spotted on solid agar plate containing galactose and galactose with apomophine (50 μM). Pictures were taken after 60–70 h D and E. Effect of Apomorphine HCl on cholera toxicity in yeast: Secondary cultures of BY4741/ ctxA -HO and vector control were set up at starting OD 600 0.05 in glucose and galactose media with different concentrations of apomorphine and were monitored for 27 hrs. OD 600 was checked every 3 h. The data shown here is collected from 3 biological triplicates (D). At the end point of liquid growth assay, the cultures were serially diluted (10 0 , 10 2 , 10 3 , 10 4 ) and were spotted on glucose and galactose plates. Pictures were taken after 60–70 hrs (E). Liquid growth assay.

Journal: PLOS ONE

Article Title: Administration of novobiocin and apomorphine mitigates cholera toxin mediated cellular toxicity: Lessons from cholera toxin yeast model system

doi: 10.1371/journal.pone.0315052

Figure Lengend Snippet: A. Schematic of high-through put screening: Pre-induced cultures of BY4741/ ctxA /pGML10 and BY4741/pGML10 as a control were used. These cultures were evenly distributed in 96 well plates, then compounds from the library plate were added at the final concentrations of 20 μM. Both plates were incubated for 24 h at 30°C and were then spotted on glucose and galactose plates for readouts. B. Schematic representation of the outcome of the small molecule library screening. Structures of the few positive hits from secondary screen are shown. C. Tertiary screening of Apomorphine HCl: Pre-induced cultures of BY4741/ ctxA /pGML10 and BY4741/pGML10 as a control were spotted on solid agar plate containing galactose and galactose with apomophine (50 μM). Pictures were taken after 60–70 h D and E. Effect of Apomorphine HCl on cholera toxicity in yeast: Secondary cultures of BY4741/ ctxA -HO and vector control were set up at starting OD 600 0.05 in glucose and galactose media with different concentrations of apomorphine and were monitored for 27 hrs. OD 600 was checked every 3 h. The data shown here is collected from 3 biological triplicates (D). At the end point of liquid growth assay, the cultures were serially diluted (10 0 , 10 2 , 10 3 , 10 4 ) and were spotted on glucose and galactose plates. Pictures were taken after 60–70 hrs (E). Liquid growth assay.

Article Snippet: Spectrum collection small molecule library (MicroSource Discovery Systems Inc., Gaylordsville) was used to screen molecules against cholera toxin in yeast model. Overnight grown cultures were used to set up secondary cultures of BY4741/pGML10 and BY4741/ctxA no signal/pGML10 at A 600 nm = 0.15 in YNB-Glucose-HMU media.

Techniques: Control, Incubation, Library Screening, Plasmid Preparation, Growth Assay