sox2  (Musashi Engineering Inc)

Journal: Stem Cell Research & Therapy

Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing

doi: 10.1186/s13287-026-05174-3

Figure Lengend Snippet: Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% SSEA4/TRA1-60, G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)

Article Snippet: Subsequently, cells were incubated in inside fix solution of the Inside Stain Kit (#130-090-477, Miltenyi Biotec, Bergisch Gladbach, Germany) for 20 min at RT and centrifuged at 200xg for 5 min. After washing in 500 μl PEB buffer and centrifugation at 200xg cells were stained with the nuclear stem cell markers OCT3/4 (1:50, #130-117-821, Miltenyi Biotec, Bergisch Gladbach, Germany) and SOX2 (1:50, #130-120-790, Miltenyi Biotec, Bergisch Gladbach, Germany) in inside perm solution for 10 min at RT.

Techniques: Virus, Derivative Assay, Staining, Generated, Flow Cytometry, Control, Expressing

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: Upregulation of SOX2 in LUAD. (A) Differential expression of SOX2 (log2 (TPM+1)) in LUAD was analyzed using the TCGA-LUAD dataset; (B) qRT-PCR was conducted to assess the mRNA expression levels of SOX2 in LUAD tissues and adjacent normal tissues; (C) Western blotting was conducted to assess the protein expression levels of SOX2 in LUAD tissues and adjacent normal tissues; (D) The mRNA expression of SOX2 was measured in human normal bronchial epithelial cells (BEAS-2B) and LUAD cell lines (A549, HCC827) by qRT-PCR. (E) The protein expression of SOX2 was measured in human normal bronchial epithelial cells (BEAS-2B) and LUAD cell lines (A549, HCC827) by Western blotting. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Quantitative Proteomics, Quantitative RT-PCR, Expressing, Western Blot

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: SOX2 knockdown attenuates LUAD malignancy. A549 cells were stratified into two groups: si-NC and si-SOX2. (A) Transfection efficiency was confirmed by qRT-PCR in each group; (B) Western blotting was used to detect the protein expression of SOX2. (C) Colony formation assays were used to evaluate cell proliferation in each group; (D) Migration and invasion were analyzed using Transwell assays in each group; (E) Apoptosis was detected by flow cytometry in each group. (F) Cell cycle was detected by flow cytometry in each group. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Knockdown, Transfection, Quantitative RT-PCR, Western Blot, Expressing, Migration, Flow Cytometry

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: SOX2 affects LUAD progression through the Wnt/β-catenin pathway. A549 cells were grouped into four categories: si-NC + DMSO, si-SOX2 + DMSO, and si-SOX2 + Wnt/β-catenin agonist 3. (A) Western blotting was used to detect the protein expression of SOX2, β-catenin, and c-Myc; (B,C) : Proliferation was evaluated by colony formation assays in each group; (D) Migration and invasion were analyzed using Transwell in each group; (E) Apoptosis was detected by flow cytometry in each group. (F) Cell cycle was detected by flow cytometry in each group. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Western Blot, Expressing, Migration, Flow Cytometry

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: WTAP-mediated m 6 A methylation promotes SOX2 mRNA decay. (A) m 6 A modification sites of SOX2 targeted by WTAP were predicted using bioinformatics tools; (B) Differential expression analysis of WTAP (log2 (TPM+1)) in LUAD was conducted based on the TCGA-LUAD dataset; (C) qRT-PCR was used to measure the mRNA expression levels of WTAP in human normal lung epithelial cells (BEAS-2B) and LUAD cells (A549, HCC827). (D,E) Western blotting was used to measure the protein expression levels of WTAP in human normal lung epithelial cells (BEAS-2B) and LUAD cells (A549, HCC827). A549 cells were assigned to two groups: oe-NC and oe-WTAP. (F) The mRNA expression of WTAP in each group was detected by qRT-PCR; (G) The mRNA expression levels of SOX2 in each group were measured by qRT-PCR; (H) Western blotting was used to measure the protein expression levels of SOX2. (I) m 6 A methylation levels in each group were detected by dot-blot assays; (J) The mRNA expression of SOX2 in each group was detected by MeRIP; (K) The mRNA expression of SOX2 in each group was detected by RIP; (L) The mRNA stability of SOX2 in each group was assessed by qRT-PCR. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Methylation, Modification, Quantitative Proteomics, Quantitative RT-PCR, Expressing, Western Blot, Dot Blot

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: WTAP affects the malignant progression of LUAD through the SOX2-mediated Wnt/β-catenin pathway. A549 cells were grouped into three categories: oe-NC + oe-NC, oe-NC + oe-SOX2, and oe-WTAP + oe-SOX2. (A) Western blotting was used to detect the protein expression levels of SOX2, β-catenin, and c-Myc in each group; (B,C) : Colony formation assays were performed to evaluate the proliferative capacity of each group; (D) Migration and invasion abilities were analyzed using Transwell assays in each group; (E) Apoptosis was detected by flow cytometry in each group. (F) Cell cycle was detected by flow cytometry in each group. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Western Blot, Expressing, Migration, Flow Cytometry

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: Upregulation of SOX2 in LUAD. (A) Differential expression of SOX2 (log2 (TPM+1)) in LUAD was analyzed using the TCGA-LUAD dataset; (B) qRT-PCR was conducted to assess the mRNA expression levels of SOX2 in LUAD tissues and adjacent normal tissues; (C) Western blotting was conducted to assess the protein expression levels of SOX2 in LUAD tissues and adjacent normal tissues; (D) The mRNA expression of SOX2 was measured in human normal bronchial epithelial cells (BEAS-2B) and LUAD cell lines (A549, HCC827) by qRT-PCR. (E) The protein expression of SOX2 was measured in human normal bronchial epithelial cells (BEAS-2B) and LUAD cell lines (A549, HCC827) by Western blotting. * P < 0.05.

Article Snippet: To assess the effects of WTAP on SOX2 mRNA stability, A549 cells were transfected with oe-NC or oe-WTAP plasmids with 5 μg/mL actinomycin D (MCE, USA) to block new RNA synthesis and measured SOX2 mRNA levels by qRT-PCR.

Techniques: Quantitative Proteomics, Quantitative RT-PCR, Expressing, Western Blot

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: SOX2 knockdown attenuates LUAD malignancy. A549 cells were stratified into two groups: si-NC and si-SOX2. (A) Transfection efficiency was confirmed by qRT-PCR in each group; (B) Western blotting was used to detect the protein expression of SOX2. (C) Colony formation assays were used to evaluate cell proliferation in each group; (D) Migration and invasion were analyzed using Transwell assays in each group; (E) Apoptosis was detected by flow cytometry in each group. (F) Cell cycle was detected by flow cytometry in each group. * P < 0.05.

Article Snippet: To assess the effects of WTAP on SOX2 mRNA stability, A549 cells were transfected with oe-NC or oe-WTAP plasmids with 5 μg/mL actinomycin D (MCE, USA) to block new RNA synthesis and measured SOX2 mRNA levels by qRT-PCR.

Techniques: Knockdown, Transfection, Quantitative RT-PCR, Western Blot, Expressing, Migration, Flow Cytometry

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: SOX2 affects LUAD progression through the Wnt/β-catenin pathway. A549 cells were grouped into four categories: si-NC + DMSO, si-SOX2 + DMSO, and si-SOX2 + Wnt/β-catenin agonist 3. (A) Western blotting was used to detect the protein expression of SOX2, β-catenin, and c-Myc; (B,C) : Proliferation was evaluated by colony formation assays in each group; (D) Migration and invasion were analyzed using Transwell in each group; (E) Apoptosis was detected by flow cytometry in each group. (F) Cell cycle was detected by flow cytometry in each group. * P < 0.05.

Article Snippet: To assess the effects of WTAP on SOX2 mRNA stability, A549 cells were transfected with oe-NC or oe-WTAP plasmids with 5 μg/mL actinomycin D (MCE, USA) to block new RNA synthesis and measured SOX2 mRNA levels by qRT-PCR.

Techniques: Western Blot, Expressing, Migration, Flow Cytometry

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: WTAP-mediated m 6 A methylation promotes SOX2 mRNA decay. (A) m 6 A modification sites of SOX2 targeted by WTAP were predicted using bioinformatics tools; (B) Differential expression analysis of WTAP (log2 (TPM+1)) in LUAD was conducted based on the TCGA-LUAD dataset; (C) qRT-PCR was used to measure the mRNA expression levels of WTAP in human normal lung epithelial cells (BEAS-2B) and LUAD cells (A549, HCC827). (D,E) Western blotting was used to measure the protein expression levels of WTAP in human normal lung epithelial cells (BEAS-2B) and LUAD cells (A549, HCC827). A549 cells were assigned to two groups: oe-NC and oe-WTAP. (F) The mRNA expression of WTAP in each group was detected by qRT-PCR; (G) The mRNA expression levels of SOX2 in each group were measured by qRT-PCR; (H) Western blotting was used to measure the protein expression levels of SOX2. (I) m 6 A methylation levels in each group were detected by dot-blot assays; (J) The mRNA expression of SOX2 in each group was detected by MeRIP; (K) The mRNA expression of SOX2 in each group was detected by RIP; (L) The mRNA stability of SOX2 in each group was assessed by qRT-PCR. * P < 0.05.

Article Snippet: To assess the effects of WTAP on SOX2 mRNA stability, A549 cells were transfected with oe-NC or oe-WTAP plasmids with 5 μg/mL actinomycin D (MCE, USA) to block new RNA synthesis and measured SOX2 mRNA levels by qRT-PCR.

Techniques: Methylation, Modification, Quantitative Proteomics, Quantitative RT-PCR, Expressing, Western Blot, Dot Blot

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: WTAP affects the malignant progression of LUAD through the SOX2-mediated Wnt/β-catenin pathway. A549 cells were grouped into three categories: oe-NC + oe-NC, oe-NC + oe-SOX2, and oe-WTAP + oe-SOX2. (A) Western blotting was used to detect the protein expression levels of SOX2, β-catenin, and c-Myc in each group; (B,C) : Colony formation assays were performed to evaluate the proliferative capacity of each group; (D) Migration and invasion abilities were analyzed using Transwell assays in each group; (E) Apoptosis was detected by flow cytometry in each group. (F) Cell cycle was detected by flow cytometry in each group. * P < 0.05.

Article Snippet: To assess the effects of WTAP on SOX2 mRNA stability, A549 cells were transfected with oe-NC or oe-WTAP plasmids with 5 μg/mL actinomycin D (MCE, USA) to block new RNA synthesis and measured SOX2 mRNA levels by qRT-PCR.

Techniques: Western Blot, Expressing, Migration, Flow Cytometry

Journal: Cell Reports Medicine

Article Title: An autochthonous CRISPR activation screening platform for characterizing tissue-specific oncogene selection

doi: 10.1016/j.xcrm.2026.102759

Figure Lengend Snippet: Sox2 activation accelerates lung tumor initiation and progression (A) Timeline of the Sox2 activation experiment. (B) Representative macroscopic images and corresponding computed tomography (CT) scans of non-targeted (NT) and Sox2-activated (sgSox2) PPKS lungs at 8 weeks. Scale bar, 1 cm. (C) Relative lung weights for NT and sgSox2 mice at 8 weeks ( n = 8 biological replicates/group). (D) CT voxel attenuation for NT and sgSox2 lungs at 8 weeks ( n = 11–12 biological replicates/group). (E) Histology, IHC (dCas9VP64 and Ki-67), and Alcian blue staining of NT and sgSox2 lungs at 8 weeks. Scale bars, 150 μm. (F) Quantification of dCas9VP64+ cells in NT and sgSox2 lungs at 8 weeks ( n = 8 biological replicates/group). (G) Quantification of Ki-67-positive cells in NT and sgSox2 lungs at 8 weeks ( n = 8 biological replicates/group). (H) Kaplan-Meier survival plot for NT (median survival 144 days) and sgSox2 (median survival 83 days) mice ( n = 6–7 biological replicates). Statistical significance for pairwise comparisons using Student’s two-tailed t test for bar graphs and log rank (Mantel-Cox) for survival. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Error bars indicate SEM. See also .

Article Snippet: For profiling of Sox2-activated lung tumors, stranded mRNA (polyA-selected) libraries were generated and sequenced by Novogene.

Techniques: Activation Assay, Computed Tomography, Staining, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: An autochthonous CRISPR activation screening platform for characterizing tissue-specific oncogene selection

doi: 10.1016/j.xcrm.2026.102759

Figure Lengend Snippet: Delineating Sox2-mediated transcriptomic reprogramming (A) Schematic of transcriptomic profiling ( n = 4 biological replicates). (B) Heatmap of all differentially expressed genes at survival endpoint (sgSox2 vs. NT). (C) Expression of Sox2 in Sox2-activated and control tumors ( n = 4 biological replicates). Error bars indicate SEM. (D) IHC of SOX2 in Sox2-activated and control lungs at 8 weeks. Scale bar, 150 μm. (E) Quantification of SOX2 IHC ( n = 4–5 biological replicates). Error bars indicate SEM. (F) Heatmap of LUSC- and LUAD-associated transcripts in Sox2-activated and control tumors. (G) Heatmap of transcripts associated with mucinous cancer and gastric metaplasia in Sox2-activated and control tumors ( n = 4 biological replicates). (H) IHC of NKX2-1, TFF1, and MUC5AC. Scale bar, 150 μm. (I) Quantification of NKX2-1, TFF1, and MUC5AC IHC at 8 weeks and survival endpoint (n = 4–5 biological replicates). EP, survival endpoint. (J) Volcano plot of mucin and gastric metaplasia genes in SOX2-high vs. -low KRAS-mutant human LUAD (TCGA-LUAD KRAS-mutant cohort, n = 89 cases). Statistical significance for pairwise comparisons using Student’s two-tailed t test for bar graphs and for multiple comparisons using one-way ANOVA with multiple comparisons correction. For differences in gene expression, adjusted p values ( q values) from DEseq2 were used. ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. See also .

Article Snippet: For profiling of Sox2-activated lung tumors, stranded mRNA (polyA-selected) libraries were generated and sequenced by Novogene.

Techniques: Expressing, Control, Mutagenesis, Two Tailed Test, Gene Expression