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software and algorithms geneious r11  (Biomatters Ltd)

 
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    Biomatters Ltd software and algorithms geneious r11
    Software And Algorithms Geneious R11, supplied by Biomatters Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software%2C+algorithm+geneious/geneious+software/pm40081380-615-26-42
    Average 90 stars, based on 1 article reviews
    software and algorithms geneious r11 - by Bioz Stars, 2026-09
    90/100 stars

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    Recombinant:

    Article Title: COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.32572 12 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) HEK293-Flp-InTM T-RexTM (HEK293T)-COX16-/- COX16 OE This paper N/A Cell line generated as described in Materials and methods Transfected construct (Homo sapiens) pX330-COX16 gRNA This paper N/A cloning described in Materials and methods Transfected construct (Homo sapiens) pEGFPN1 Clonetech N/A Transfected construct (Homo sapiens) pCDNA5-COX16 This paper N/A Construct generated from amplifying COX16 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-FLAG This paper N/A Construct generated from amplifying COA6 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-W59C-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5COA6-FLAG Transfected construct (Homo sapiens) pCDNA5-COA6-W66R-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5-COA6 -FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1 This paper N/A Construct generated from amplifying SCO1 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-SCO1-G132S-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1-P174L-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Biological sampleN/A Antibody MITRAC12 self made PRAB3761 (1:1000) Antibody C12ORF62 self made PRAB4844 (1:500) Antibody MITRAC7 self made PRAB4843 (1:500) Antibody COX16 Proteintech RRID:AB_10666854 (1:1000) Antibody COX1 self made PRAB2035 (1:2000) Antibody COX4-1 self made PRAB1522 (1:2000) Antibody MRPL23 self made PRAB1716 (1:500) Antibody MRPL1 self made PRAB4969 (1:500) Antibody TOM70 self made PRAB3280 (1:1000) Antibody TACO1 self made PRAB3627 (1:500) Antibody MITRAC15 self made PRAB4814 (1:500) Antibody FLAG Sigma Aldrich RRID:AB_259529 (1:2000) Antibody COX2 Abcam ab110258 (1:2000) Antibody TIM21 self made PRAB3674 (1:2000) Antibody VDAC self made PRAB1515 (1:1500) Antibody SDHA self made PRAB4978 (1:2000) Antibody Rieske self made PRAB1512 (1:2000) Antibody ATP5B self made PRAB4826 (1:10000) Antibody TIM44 Proteintech RRID:AB_2204679 (1:2500) Antibody NDUFB8 self made PRAB3764 (1:500) Antibody NDUFA9 self made PRAB1524 (1:500) Continued on next page Aich et al. eLife 2018;7:e32572. .. DOI: https://doi.org/10.7554/eLife.32572 13 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody LETM1 self made PRAB538 (1:5000) Antibody TIM23 self made PRAB1527 (1:2000) Antibody SCO1 self made PRAB4980 (1:500) Antibody COA6 self made PRAB5007 (1:500) Antibody COX6C self made PRAB4950 (1:2000) Antibody SCO2 self made PRAB4982 (1:500) Antibody FAM36A self made PRAB4490 (1:500) Antibody SURF1 self made PRAB1528 (1:1000) Recombinant DNA reagent QuikChange Site-Directed Mutagenesis Kit Agilent 210515 Recombinant DNA reagent KOD Hot Start DNA Polymerase Merck 71086–3 Recombinant DNA reagent First Strand cDNA Synthesis kit ThermoFisher Scientific K1612 Commercial assay or kit Human Complex IV activity kit Abcam ab109910 Chemical compound, drug GeneJuice Merck 70967–3 Chemical compound, drug Anti-FLAG M2 Affinity Gel Sigma-Aldrich A2220 Chemical compound, drug Trizol ThermoFisher Scientific 15596026 Chemical compound, drug Protein-A SepharoseTM CL-4B GE Healthcare 17-0963-03 Chemical compound, drug [35S]methionine Hartmann Analytic SCM-01 Chemical compound, drug Emetine dihydrochloride hydrate Sigma-Aldrich 219282 Chemical compound, drug Anisomycin AppliChem A7650,0025 Software, algorithm ImageQuantTL 7.0 software GE Healthcare RRID:SCR_014246 Software, algorithm ImageJ 1.47 v NIH RRID:SCR_003070 Software, algorithm Geneious Biomatters Ltd RRID:SCR_010519 Software, algorithm Prism5 GraphPad Software RRID:SCR_015807 .. HEK Flp-In T-REx 293 (Invitrogen, Carlsbad, CA) (HEK-293T) were cultured in DMEM, supplemented with 10% [v/v] fetal bovine serum (FBS) (GIBCO, Invitrogen), 2 mM L-glutamine and 50 mg/ml uridine at 37 ̊C under a 5% CO2 humidified atmosphere.

    Mutagenesis:

    Article Title: COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.32572 12 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) HEK293-Flp-InTM T-RexTM (HEK293T)-COX16-/- COX16 OE This paper N/A Cell line generated as described in Materials and methods Transfected construct (Homo sapiens) pX330-COX16 gRNA This paper N/A cloning described in Materials and methods Transfected construct (Homo sapiens) pEGFPN1 Clonetech N/A Transfected construct (Homo sapiens) pCDNA5-COX16 This paper N/A Construct generated from amplifying COX16 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-FLAG This paper N/A Construct generated from amplifying COA6 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-W59C-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5COA6-FLAG Transfected construct (Homo sapiens) pCDNA5-COA6-W66R-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5-COA6 -FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1 This paper N/A Construct generated from amplifying SCO1 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-SCO1-G132S-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1-P174L-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Biological sampleN/A Antibody MITRAC12 self made PRAB3761 (1:1000) Antibody C12ORF62 self made PRAB4844 (1:500) Antibody MITRAC7 self made PRAB4843 (1:500) Antibody COX16 Proteintech RRID:AB_10666854 (1:1000) Antibody COX1 self made PRAB2035 (1:2000) Antibody COX4-1 self made PRAB1522 (1:2000) Antibody MRPL23 self made PRAB1716 (1:500) Antibody MRPL1 self made PRAB4969 (1:500) Antibody TOM70 self made PRAB3280 (1:1000) Antibody TACO1 self made PRAB3627 (1:500) Antibody MITRAC15 self made PRAB4814 (1:500) Antibody FLAG Sigma Aldrich RRID:AB_259529 (1:2000) Antibody COX2 Abcam ab110258 (1:2000) Antibody TIM21 self made PRAB3674 (1:2000) Antibody VDAC self made PRAB1515 (1:1500) Antibody SDHA self made PRAB4978 (1:2000) Antibody Rieske self made PRAB1512 (1:2000) Antibody ATP5B self made PRAB4826 (1:10000) Antibody TIM44 Proteintech RRID:AB_2204679 (1:2500) Antibody NDUFB8 self made PRAB3764 (1:500) Antibody NDUFA9 self made PRAB1524 (1:500) Continued on next page Aich et al. eLife 2018;7:e32572. .. DOI: https://doi.org/10.7554/eLife.32572 13 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody LETM1 self made PRAB538 (1:5000) Antibody TIM23 self made PRAB1527 (1:2000) Antibody SCO1 self made PRAB4980 (1:500) Antibody COA6 self made PRAB5007 (1:500) Antibody COX6C self made PRAB4950 (1:2000) Antibody SCO2 self made PRAB4982 (1:500) Antibody FAM36A self made PRAB4490 (1:500) Antibody SURF1 self made PRAB1528 (1:1000) Recombinant DNA reagent QuikChange Site-Directed Mutagenesis Kit Agilent 210515 Recombinant DNA reagent KOD Hot Start DNA Polymerase Merck 71086–3 Recombinant DNA reagent First Strand cDNA Synthesis kit ThermoFisher Scientific K1612 Commercial assay or kit Human Complex IV activity kit Abcam ab109910 Chemical compound, drug GeneJuice Merck 70967–3 Chemical compound, drug Anti-FLAG M2 Affinity Gel Sigma-Aldrich A2220 Chemical compound, drug Trizol ThermoFisher Scientific 15596026 Chemical compound, drug Protein-A SepharoseTM CL-4B GE Healthcare 17-0963-03 Chemical compound, drug [35S]methionine Hartmann Analytic SCM-01 Chemical compound, drug Emetine dihydrochloride hydrate Sigma-Aldrich 219282 Chemical compound, drug Anisomycin AppliChem A7650,0025 Software, algorithm ImageQuantTL 7.0 software GE Healthcare RRID:SCR_014246 Software, algorithm ImageJ 1.47 v NIH RRID:SCR_003070 Software, algorithm Geneious Biomatters Ltd RRID:SCR_010519 Software, algorithm Prism5 GraphPad Software RRID:SCR_015807 .. HEK Flp-In T-REx 293 (Invitrogen, Carlsbad, CA) (HEK-293T) were cultured in DMEM, supplemented with 10% [v/v] fetal bovine serum (FBS) (GIBCO, Invitrogen), 2 mM L-glutamine and 50 mg/ml uridine at 37 ̊C under a 5% CO2 humidified atmosphere.

    cDNA Synthesis:

    Article Title: COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.32572 12 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) HEK293-Flp-InTM T-RexTM (HEK293T)-COX16-/- COX16 OE This paper N/A Cell line generated as described in Materials and methods Transfected construct (Homo sapiens) pX330-COX16 gRNA This paper N/A cloning described in Materials and methods Transfected construct (Homo sapiens) pEGFPN1 Clonetech N/A Transfected construct (Homo sapiens) pCDNA5-COX16 This paper N/A Construct generated from amplifying COX16 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-FLAG This paper N/A Construct generated from amplifying COA6 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-W59C-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5COA6-FLAG Transfected construct (Homo sapiens) pCDNA5-COA6-W66R-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5-COA6 -FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1 This paper N/A Construct generated from amplifying SCO1 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-SCO1-G132S-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1-P174L-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Biological sampleN/A Antibody MITRAC12 self made PRAB3761 (1:1000) Antibody C12ORF62 self made PRAB4844 (1:500) Antibody MITRAC7 self made PRAB4843 (1:500) Antibody COX16 Proteintech RRID:AB_10666854 (1:1000) Antibody COX1 self made PRAB2035 (1:2000) Antibody COX4-1 self made PRAB1522 (1:2000) Antibody MRPL23 self made PRAB1716 (1:500) Antibody MRPL1 self made PRAB4969 (1:500) Antibody TOM70 self made PRAB3280 (1:1000) Antibody TACO1 self made PRAB3627 (1:500) Antibody MITRAC15 self made PRAB4814 (1:500) Antibody FLAG Sigma Aldrich RRID:AB_259529 (1:2000) Antibody COX2 Abcam ab110258 (1:2000) Antibody TIM21 self made PRAB3674 (1:2000) Antibody VDAC self made PRAB1515 (1:1500) Antibody SDHA self made PRAB4978 (1:2000) Antibody Rieske self made PRAB1512 (1:2000) Antibody ATP5B self made PRAB4826 (1:10000) Antibody TIM44 Proteintech RRID:AB_2204679 (1:2500) Antibody NDUFB8 self made PRAB3764 (1:500) Antibody NDUFA9 self made PRAB1524 (1:500) Continued on next page Aich et al. eLife 2018;7:e32572. .. DOI: https://doi.org/10.7554/eLife.32572 13 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody LETM1 self made PRAB538 (1:5000) Antibody TIM23 self made PRAB1527 (1:2000) Antibody SCO1 self made PRAB4980 (1:500) Antibody COA6 self made PRAB5007 (1:500) Antibody COX6C self made PRAB4950 (1:2000) Antibody SCO2 self made PRAB4982 (1:500) Antibody FAM36A self made PRAB4490 (1:500) Antibody SURF1 self made PRAB1528 (1:1000) Recombinant DNA reagent QuikChange Site-Directed Mutagenesis Kit Agilent 210515 Recombinant DNA reagent KOD Hot Start DNA Polymerase Merck 71086–3 Recombinant DNA reagent First Strand cDNA Synthesis kit ThermoFisher Scientific K1612 Commercial assay or kit Human Complex IV activity kit Abcam ab109910 Chemical compound, drug GeneJuice Merck 70967–3 Chemical compound, drug Anti-FLAG M2 Affinity Gel Sigma-Aldrich A2220 Chemical compound, drug Trizol ThermoFisher Scientific 15596026 Chemical compound, drug Protein-A SepharoseTM CL-4B GE Healthcare 17-0963-03 Chemical compound, drug [35S]methionine Hartmann Analytic SCM-01 Chemical compound, drug Emetine dihydrochloride hydrate Sigma-Aldrich 219282 Chemical compound, drug Anisomycin AppliChem A7650,0025 Software, algorithm ImageQuantTL 7.0 software GE Healthcare RRID:SCR_014246 Software, algorithm ImageJ 1.47 v NIH RRID:SCR_003070 Software, algorithm Geneious Biomatters Ltd RRID:SCR_010519 Software, algorithm Prism5 GraphPad Software RRID:SCR_015807 .. HEK Flp-In T-REx 293 (Invitrogen, Carlsbad, CA) (HEK-293T) were cultured in DMEM, supplemented with 10% [v/v] fetal bovine serum (FBS) (GIBCO, Invitrogen), 2 mM L-glutamine and 50 mg/ml uridine at 37 ̊C under a 5% CO2 humidified atmosphere.

    Activity Assay:

    Article Title: COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.32572 12 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) HEK293-Flp-InTM T-RexTM (HEK293T)-COX16-/- COX16 OE This paper N/A Cell line generated as described in Materials and methods Transfected construct (Homo sapiens) pX330-COX16 gRNA This paper N/A cloning described in Materials and methods Transfected construct (Homo sapiens) pEGFPN1 Clonetech N/A Transfected construct (Homo sapiens) pCDNA5-COX16 This paper N/A Construct generated from amplifying COX16 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-FLAG This paper N/A Construct generated from amplifying COA6 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-W59C-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5COA6-FLAG Transfected construct (Homo sapiens) pCDNA5-COA6-W66R-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5-COA6 -FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1 This paper N/A Construct generated from amplifying SCO1 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-SCO1-G132S-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1-P174L-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Biological sampleN/A Antibody MITRAC12 self made PRAB3761 (1:1000) Antibody C12ORF62 self made PRAB4844 (1:500) Antibody MITRAC7 self made PRAB4843 (1:500) Antibody COX16 Proteintech RRID:AB_10666854 (1:1000) Antibody COX1 self made PRAB2035 (1:2000) Antibody COX4-1 self made PRAB1522 (1:2000) Antibody MRPL23 self made PRAB1716 (1:500) Antibody MRPL1 self made PRAB4969 (1:500) Antibody TOM70 self made PRAB3280 (1:1000) Antibody TACO1 self made PRAB3627 (1:500) Antibody MITRAC15 self made PRAB4814 (1:500) Antibody FLAG Sigma Aldrich RRID:AB_259529 (1:2000) Antibody COX2 Abcam ab110258 (1:2000) Antibody TIM21 self made PRAB3674 (1:2000) Antibody VDAC self made PRAB1515 (1:1500) Antibody SDHA self made PRAB4978 (1:2000) Antibody Rieske self made PRAB1512 (1:2000) Antibody ATP5B self made PRAB4826 (1:10000) Antibody TIM44 Proteintech RRID:AB_2204679 (1:2500) Antibody NDUFB8 self made PRAB3764 (1:500) Antibody NDUFA9 self made PRAB1524 (1:500) Continued on next page Aich et al. eLife 2018;7:e32572. .. DOI: https://doi.org/10.7554/eLife.32572 13 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody LETM1 self made PRAB538 (1:5000) Antibody TIM23 self made PRAB1527 (1:2000) Antibody SCO1 self made PRAB4980 (1:500) Antibody COA6 self made PRAB5007 (1:500) Antibody COX6C self made PRAB4950 (1:2000) Antibody SCO2 self made PRAB4982 (1:500) Antibody FAM36A self made PRAB4490 (1:500) Antibody SURF1 self made PRAB1528 (1:1000) Recombinant DNA reagent QuikChange Site-Directed Mutagenesis Kit Agilent 210515 Recombinant DNA reagent KOD Hot Start DNA Polymerase Merck 71086–3 Recombinant DNA reagent First Strand cDNA Synthesis kit ThermoFisher Scientific K1612 Commercial assay or kit Human Complex IV activity kit Abcam ab109910 Chemical compound, drug GeneJuice Merck 70967–3 Chemical compound, drug Anti-FLAG M2 Affinity Gel Sigma-Aldrich A2220 Chemical compound, drug Trizol ThermoFisher Scientific 15596026 Chemical compound, drug Protein-A SepharoseTM CL-4B GE Healthcare 17-0963-03 Chemical compound, drug [35S]methionine Hartmann Analytic SCM-01 Chemical compound, drug Emetine dihydrochloride hydrate Sigma-Aldrich 219282 Chemical compound, drug Anisomycin AppliChem A7650,0025 Software, algorithm ImageQuantTL 7.0 software GE Healthcare RRID:SCR_014246 Software, algorithm ImageJ 1.47 v NIH RRID:SCR_003070 Software, algorithm Geneious Biomatters Ltd RRID:SCR_010519 Software, algorithm Prism5 GraphPad Software RRID:SCR_015807 .. HEK Flp-In T-REx 293 (Invitrogen, Carlsbad, CA) (HEK-293T) were cultured in DMEM, supplemented with 10% [v/v] fetal bovine serum (FBS) (GIBCO, Invitrogen), 2 mM L-glutamine and 50 mg/ml uridine at 37 ̊C under a 5% CO2 humidified atmosphere.

    Software:

    Article Title: COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.32572 12 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) HEK293-Flp-InTM T-RexTM (HEK293T)-COX16-/- COX16 OE This paper N/A Cell line generated as described in Materials and methods Transfected construct (Homo sapiens) pX330-COX16 gRNA This paper N/A cloning described in Materials and methods Transfected construct (Homo sapiens) pEGFPN1 Clonetech N/A Transfected construct (Homo sapiens) pCDNA5-COX16 This paper N/A Construct generated from amplifying COX16 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-FLAG This paper N/A Construct generated from amplifying COA6 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-COA6-W59C-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5COA6-FLAG Transfected construct (Homo sapiens) pCDNA5-COA6-W66R-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5-COA6 -FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1 This paper N/A Construct generated from amplifying SCO1 from WT HEK cDNA Transfected construct (Homo sapiens) pCDNA5-SCO1-G132S-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Transfected construct (Homo sapiens) pCDNA5-SCO1-P174L-FLAG This paper N/A Construct generated by mutagenesis of pCDNA5SCO1-FLAG Biological sampleN/A Antibody MITRAC12 self made PRAB3761 (1:1000) Antibody C12ORF62 self made PRAB4844 (1:500) Antibody MITRAC7 self made PRAB4843 (1:500) Antibody COX16 Proteintech RRID:AB_10666854 (1:1000) Antibody COX1 self made PRAB2035 (1:2000) Antibody COX4-1 self made PRAB1522 (1:2000) Antibody MRPL23 self made PRAB1716 (1:500) Antibody MRPL1 self made PRAB4969 (1:500) Antibody TOM70 self made PRAB3280 (1:1000) Antibody TACO1 self made PRAB3627 (1:500) Antibody MITRAC15 self made PRAB4814 (1:500) Antibody FLAG Sigma Aldrich RRID:AB_259529 (1:2000) Antibody COX2 Abcam ab110258 (1:2000) Antibody TIM21 self made PRAB3674 (1:2000) Antibody VDAC self made PRAB1515 (1:1500) Antibody SDHA self made PRAB4978 (1:2000) Antibody Rieske self made PRAB1512 (1:2000) Antibody ATP5B self made PRAB4826 (1:10000) Antibody TIM44 Proteintech RRID:AB_2204679 (1:2500) Antibody NDUFB8 self made PRAB3764 (1:500) Antibody NDUFA9 self made PRAB1524 (1:500) Continued on next page Aich et al. eLife 2018;7:e32572. .. DOI: https://doi.org/10.7554/eLife.32572 13 of 18 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody LETM1 self made PRAB538 (1:5000) Antibody TIM23 self made PRAB1527 (1:2000) Antibody SCO1 self made PRAB4980 (1:500) Antibody COA6 self made PRAB5007 (1:500) Antibody COX6C self made PRAB4950 (1:2000) Antibody SCO2 self made PRAB4982 (1:500) Antibody FAM36A self made PRAB4490 (1:500) Antibody SURF1 self made PRAB1528 (1:1000) Recombinant DNA reagent QuikChange Site-Directed Mutagenesis Kit Agilent 210515 Recombinant DNA reagent KOD Hot Start DNA Polymerase Merck 71086–3 Recombinant DNA reagent First Strand cDNA Synthesis kit ThermoFisher Scientific K1612 Commercial assay or kit Human Complex IV activity kit Abcam ab109910 Chemical compound, drug GeneJuice Merck 70967–3 Chemical compound, drug Anti-FLAG M2 Affinity Gel Sigma-Aldrich A2220 Chemical compound, drug Trizol ThermoFisher Scientific 15596026 Chemical compound, drug Protein-A SepharoseTM CL-4B GE Healthcare 17-0963-03 Chemical compound, drug [35S]methionine Hartmann Analytic SCM-01 Chemical compound, drug Emetine dihydrochloride hydrate Sigma-Aldrich 219282 Chemical compound, drug Anisomycin AppliChem A7650,0025 Software, algorithm ImageQuantTL 7.0 software GE Healthcare RRID:SCR_014246 Software, algorithm ImageJ 1.47 v NIH RRID:SCR_003070 Software, algorithm Geneious Biomatters Ltd RRID:SCR_010519 Software, algorithm Prism5 GraphPad Software RRID:SCR_015807 .. HEK Flp-In T-REx 293 (Invitrogen, Carlsbad, CA) (HEK-293T) were cultured in DMEM, supplemented with 10% [v/v] fetal bovine serum (FBS) (GIBCO, Invitrogen), 2 mM L-glutamine and 50 mg/ml uridine at 37 ̊C under a 5% CO2 humidified atmosphere.



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    Image Search Results


    Journal: Cell chemical biology

    Article Title: Linking Genomic and Metabolomic Natural Variation Uncovers Nematode Pheromone Biosynthesis

    doi: 10.1016/j.chembiol.2018.04.004

    Figure Lengend Snippet:

    Article Snippet: Clontech Cat# 3340 Software and Algorithms Geneious ® Biomatters Limited, version 8.0.4 https://www.geneious.com/download/ easyGWAS https://easygwas.ethz.ch/ BWA Version 0.6.1-r104 https://github.com/lh3/bwa samtools Version 0.1.18 http://www.htslib.org/ PHYML Version 3.0 http://www.atgc-montpellier.fr/phyml/ Muscle Version 3.8.31 https://www.drive5.com/muscle/ Seaview Version 1:4.4.2-1 http://doua.prabi.fr/software/seaview PBcR Version 8.3rc2 http://wgs-assembler.sourceforge.net/wiki/index.php/PBcR XCMS R code Bioconductor ( Tautenhahn et al., 2012 ) http://bioconductor.org/packages/release/bioc/html/xcms.html Thermo XCalibur ThermoFisher https://www.thermofisher.com/order/catalog/product/OPTON-30487 MestReNova Mestrelab Research, version 11.0 http://mestrelab.com/download/mnova/ Open in a separate window

    Techniques: Virus, Recombinant, Extraction, Purification, Nanopore Sequencing, Isolation, Expressing, Plasmid Preparation, Software