Review



slc4a11  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Novus Biologicals slc4a11
    Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), <t>SLC4A11,</t> N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.
    Slc4a11, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc4a11/SLC4A11+Antibody/pmc13042470-323-9-11
    Average 94 stars, based on 3 article reviews
    slc4a11 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "High‐Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash‐Out Method"

    Article Title: High‐Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash‐Out Method

    Journal: MedComm

    doi: 10.1002/mco2.70650

    Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), SLC4A11, N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.
    Figure Legend Snippet: Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), SLC4A11, N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.

    Techniques Used: Generated, Control, Comparison, Gentle, Incubation, Microscopy, Immunocytochemistry, Marker, Derivative Assay

    Related Articles

    Incubation:

    Article Title: Therapeutic Potency of Induced Pluripotent Stem-Cell-Derived Corneal Endothelial-like Cells for Corneal Endothelial Dysfunction.
    Article Snippet: Human primary CECs or iPSC-derived CECs were fixed with 4% paraformaldehyde overnight at 4 ◦C, washed with PBS/0.05% Tween 20 (PBST) buffer, permeabilized in 0.5% Triton X-100, and blocked in PBST containing 1% bovine serum albumin (SigmaAldrich). .. Samples were incubated with primary antibodies against human anti-ZO-1 (1:250; Life Technologies), Na+/K+ ATPase α1 (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), SLC4A11 (1:250, Novus, Centennial, CO, USA), and N-cadherin (1:200, Cell signaling technology, Danvers, MA, USA) overnight at 4 ◦C, followed by goat anti-mouse IgG Alexa Fluor 488-conjugated and donkey anti-rabbit IgG Alexa Fluor 647-conjugated secondary antibodies (1:500; Abcam, Cambridge, UK). .. Nuclei were counterstained with 4’, 6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 min and fluorescence signals detected with either a fluorescence microscope (EVOS, Life Technologies) or a laser-scanning confocal microscope (Olympus, Tokyo, Japan).

    Article Title: Therapeutic Potency of Induced Pluripotent Stem-Cell-Derived Corneal Endothelial-like Cells for Corneal Endothelial Dysfunction.
    Article Snippet: Non-specific protein binding was blocked with 3% BSA (Sigma-Aldrich) in PBST. .. Membranes were incubated with antibodies against ZO-1 antibodies (1:1000), NCAM (1:200, R & D systems, Minneapolis, MN, USA), SLC4A11 (1:250, Novus), N-cadherin (1:200, Cell signaling technology), and β-actin (1:1000; Cell signaling technology) overnight. ..

    Article Title: High‐Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash‐Out Method
    Article Snippet: Membranes were blocked with 3% BSA (Sigma–Aldrich) in PBST to prevent nonspecific binding. .. Membranes were incubated overnight with antibodies against ZO-1 (1:1000), SLC4A11 (1:250; Novus), NCADHERIN (1:200; Cell Signaling Technology), Na+ /K+ ATPase α1 (1:200; Santa Cruz Biotechnology), human nuclear antigen (1:200; Abcam), and β-actin (1:1,000; Cell Signaling Technology). .. Membrane was washed and incubated with an HRP-conjugated anti-mouse secondary antibody (GeneTex, Hsinchu) at a 1:10,000 15 of 18 om m ons L icense TABLE 2 Primer sequences used. (A) Sequences used for RT-qPCR. (B) Sequences used for human mitochondrial DNA detection. (C) Sequences used for human Alu detection.

    Article Title: High‐Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash‐Out Method
    Article Snippet: Membranes were blocked with 3% BSA (Sigma–Aldrich) in PBST to prevent nonspecific binding. .. Membranes were incubated overnight with antibodies against ZO‐1 (1:1000), SLC4A11 (1:250; Novus), N‐CADHERIN (1:200; Cell Signaling Technology), Na + /K + ATPase α1 (1:200; Santa Cruz Biotechnology), human nuclear antigen (1:200; Abcam), and β‐actin (1:1,000; Cell Signaling Technology). .. Membrane was washed and incubated with an HRP‐conjugated anti‐mouse secondary antibody (GeneTex, Hsinchu) at a 1:10,000 dilution.



    Similar Products

    anti slc4a11 sirna ccgaaaguaccugaaguuaaagaact  (OriGene)
    94
    OriGene anti slc4a11 sirna ccgaaaguaccugaaguuaaagaact
    Anti Slc4a11 Sirna Ccgaaaguaccugaaguuaaagaact, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc4a11/Sodium+bicarbonate+transporter+like+protein+11+(SLC4A11)+Human+siRNA+Oligo+Duplex/pmc13151172-95-17-23
    Average 94 stars, based on 1 article reviews
    anti slc4a11 sirna ccgaaaguaccugaaguuaaagaact - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    slc4a11  (Novus Biologicals)
    94
    Novus Biologicals slc4a11
    Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), <t>SLC4A11,</t> N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.
    Slc4a11, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc4a11/SLC4A11+Antibody/pmc13042470-323-9-11
    Average 94 stars, based on 1 article reviews
    slc4a11 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    flag twpa obio technology n a paav6 cmv slc4a11  (Obio Technology Corp Ltd)
    86
    Obio Technology Corp Ltd flag twpa obio technology n a paav6 cmv slc4a11
    Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), <t>SLC4A11,</t> N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.
    Flag Twpa Obio Technology N A Paav6 Cmv Slc4a11, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc4a11/egfp+flag+p2a/pm41850243-292-132-133
    Average 86 stars, based on 1 article reviews
    flag twpa obio technology n a paav6 cmv slc4a11 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    slc4a11 knockout mice cyagen biosciences cat  (Cyagen Biosciences)
    94
    Cyagen Biosciences slc4a11 knockout mice cyagen biosciences cat
    Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), <t>SLC4A11,</t> N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.
    Slc4a11 Knockout Mice Cyagen Biosciences Cat, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc4a11/Slc4a11/pm41850243-308-43-46
    Average 94 stars, based on 1 article reviews
    slc4a11 knockout mice cyagen biosciences cat - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    slc4a11 knockout mice  (Cyagen Biosciences)
    94
    Cyagen Biosciences slc4a11 knockout mice
    Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), <t>SLC4A11,</t> N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.
    Slc4a11 Knockout Mice, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc4a11/Slc4a11/pm41850243-295-0-6
    Average 94 stars, based on 1 article reviews
    slc4a11 knockout mice - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    paav6 cmv slc4a11 3 × flag twpa  (Obio Technology Corp Ltd)
    86
    Obio Technology Corp Ltd paav6 cmv slc4a11 3 × flag twpa
    Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), <t>SLC4A11,</t> N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.
    Paav6 Cmv Slc4a11 3 × Flag Twpa, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc4a11/egfp+flag+p2a/pmc13006425-23-0-2
    Average 86 stars, based on 1 article reviews
    paav6 cmv slc4a11 3 × flag twpa - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    269356 slc4a11 b6n va  (Cyagen Biosciences)
    94
    Cyagen Biosciences 269356 slc4a11 b6n va
    Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), <t>SLC4A11,</t> N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.
    269356 Slc4a11 B6n Va, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc4a11/Slc4a11/pmc13006425-59-7-4
    Average 94 stars, based on 1 article reviews
    269356 slc4a11 b6n va - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), SLC4A11, N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.

    Journal: MedComm

    Article Title: High‐Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash‐Out Method

    doi: 10.1002/mco2.70650

    Figure Lengend Snippet: Characterization of corneal endothelial‐like cells (CECs) generated from induced pluripotent stem cells (iPSCs). (A) The wash‐out protocol enables more differentiated CECs to adhere at a faster rate to the VTN‐coated surface during the initial stage, while relatively immature cells with weaker attachment capacity are subsequently washed out and removed. A control culture dish without the wash‐out step is used for comparison of the initial cell seeding number. When approximately 50% of the seeded cells had attached to the culture surface, the remaining unattached cells were removed by aspirating the medium containing unattached cells, followed by a single gentle wash with PBS for less than 1 min. The culture was then replenished with fresh medium at the same volume routinely used for maintenance and returned to incubation. The wash‐out protocol was applied to the ACE2 cell population at each passage during the differentiation process and was performed once weekly upon reaching confluence, resulting in two wash‐out procedures during the 14‐day differentiation protocol and three during the 21‐day protocol. (B) Protocol for the differentiation of iPSCs into CECs. iPSCs were differentiated into CECs through either a neural crest cell (NCC) intermediate stage or directly, without the NCC stage. Phase‐contrast microscopy revealed CEC‐specific hexagonal morphology after the differentiation. Scale bars = 100 µm. (C) Immunocytochemistry for OCT4 and NANOG, iPSC markers, and CD166, a CEC marker, on iPSC colonies. No CD166‐IR was detected in the iPSC colonies. Scale bars = 50 µm (OCT4, CD166); scale bars = 20 µm (NANOG). (D) Immunocytochemistry for a group of CEC‐specific markers; zona occludens‐1 (ZO‐1; a tight‐junction protein), pump function protein Na + /K + ATPase α1 (ATP1A1), SLC4A11, N‐CADHERIN, CD166, and NCAM on iPSC‐derived CECs. Scale bars = 100 µm.

    Article Snippet: The antibodies used included human anti‐ZO‐1 (1:250; Life Technologies), SLC4A11 (1:250; Novus, Centennial, CO), N‐CADHERIN (1:200; Cell Signaling Technology, Danvers, MA), Na + /K + ATPase α1 (1:200; Santa Cruz Biotechnology, Dallas, TX), CD166 (1:500; BD Biosciences, Franklin Lakes, NJ), PITX2 (100 μg/mL; Abnova, Taipei), and NCAM (1:200; R&D Systems, Minneapolis, MN).

    Techniques: Generated, Control, Comparison, Gentle, Incubation, Microscopy, Immunocytochemistry, Marker, Derivative Assay