Journal: Genes & Diseases

Article Title: Blockade of co-inhibitory receptor immune checkpoint protein TIM3/CD366 augments the anti-cancer activity of CAR-T therapy in solid tumors: An ovarian cancer example

doi: 10.1016/j.gendis.2025.101978

Figure Lengend Snippet: Specific IFN-γ and TNF-α release of T lymphocytes transduced with TIM-3-silenced HER2-specific chimeric antigen receptor (CAR) or HER2-specific CAR. (A, B) TIM-3-silenced CAR-T cells and control T cells were co-incubated with Galectin-9 + or Galectin-9 – SKOV3 tumor cells (E:T ratio 5:1 or 10:1). At 20 h after coculture, a specific enzyme-linked immunosorbent assay was used to analyze the supernatant for IFN-γ cytokine-release. Results were presented as mean ± standard deviation. (C, D) The detection of TNF-α in the same culture supernatant. Results were presented as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Human cervical cancer cell line HeLa, lentivirus packaging cell line HEK 293TD, and human ovarian cancer cell line SKOV3 were purchased from American Type Culture Collection (Manassas, Virginia, USA) and cultured in Dulbecco's modified Eagle's medium (Invitrogen, Grand Island, New York) supplemented with 10% heat-inactivated fetal bovine serum.

Techniques: Transduction, Control, Incubation, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Genes & Diseases

Article Title: Blockade of co-inhibitory receptor immune checkpoint protein TIM3/CD366 augments the anti-cancer activity of CAR-T therapy in solid tumors: An ovarian cancer example

doi: 10.1016/j.gendis.2025.101978

Figure Lengend Snippet: TIM-3 silencing augmented the anti-tumor activity of chimeric antigen receptor-T (CAR-T) cells in vivo . 2 × 10 6 SKOV3 tumor cells expressing luciferase were intraperitoneally inoculated in a xenograft mouse model, and 7 days after inoculation, the 2 × 10 6 HER2-specific CAR-T kdTim-3 cells or CAR-T cells, or untreated T cells were intraperitoneally administered. (A, B) Tumor growth was monitored using an in vivo imaging system. (C) Survival curve of 80-day post-treatment. ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Human cervical cancer cell line HeLa, lentivirus packaging cell line HEK 293TD, and human ovarian cancer cell line SKOV3 were purchased from American Type Culture Collection (Manassas, Virginia, USA) and cultured in Dulbecco's modified Eagle's medium (Invitrogen, Grand Island, New York) supplemented with 10% heat-inactivated fetal bovine serum.

Techniques: Activity Assay, In Vivo, Expressing, Luciferase, In Vivo Imaging

Journal: bioRxiv

Article Title: Modular Albumin-Chaperoned NIR-II Nanofluorophores Enables Pan-Ovarian Cancer Imaging Across Multiscale Tumor Models

doi: 10.64898/2026.05.06.717945

Figure Lengend Snippet: (a) RNA sequencing data confirming the biomarker expression levels of FOLR1 and SPARC genes in SKOV3 and OVCAR8 tumor cells. (b) SKOV3 cell viability following BSA-FA@SP2 treatment [1-200µg/mL]. (c) IVIS fluorescence images of SKOV3 cultures after 24h treatment with DSPE-PEG@, BSA@, and BSA-FA@SP2 nanofluorophores, with (d) corresponding radiant intensities. (e) Schematic diagram showing experimental designs for time-dependent uptake studies. (f,h) IVIS fluorescence images of cellular uptake of BSA-FA@SP2 in (f) SKOV3 and (h) OVCAR8, with (g,i) corresponding radiant intensities. (j) Schematic diagram showing experimental designs for inhibition studies. (k, m) IVIS fluorescence images of cell uptake of BSA-FA@SP2 in (k) SKOV3 and (m) OVCAR8 following treatment with media (control: microcentrifuge tube 2), SPARC-blocking solution (10µM, microcentrifuge tube 3), and FOLR-blocking solution (10µM, microcentrifuge tube 4). microcentrifuge tube 1 serves as a cell media control, with (l,n) corresponding radiant intensities. All IVIS images were collected with λ ex : 675nm and λ em : 840nm.

Article Snippet: Tumor-On-Chip Cell Cluster Growth and Cell Viability: The human ovarian carcinoma cell line SKOV3 (ATCC) was maintained and expanded following standard culture protocols as previously described.

Techniques: RNA Sequencing, Biomarker Discovery, Expressing, Fluorescence, Inhibition, Control, Blocking Assay

Journal: bioRxiv

Article Title: Modular Albumin-Chaperoned NIR-II Nanofluorophores Enables Pan-Ovarian Cancer Imaging Across Multiscale Tumor Models

doi: 10.64898/2026.05.06.717945

Figure Lengend Snippet: (a) Schematic of the on-chip platform featuring a microfluidic channel mimicking leaky vasculature and a tumor cluster. (b) Representative bright-field (top) and live/dead fluorescence (bottom) images of SKOV3 cell clusters. (c) Live/dead assay fluorescence images of SKOV3 clusters following 36h perfusion of nanofluorophore treatments with (d) corresponding cell viability quantification using a MATLAB script (See Experimental Methods), n = 6. (e-g) IVIS images of SKOV3 clusters after 36h perfusion with (e) DSPE-PEG@SP2, (f) BSA@SP2, and (g) BSA-FA@SP2 with (h) respective radiant intensities, n = 6. All IVIS images were collected with λ ex : 675nm and λ em : 840nm.

Article Snippet: Tumor-On-Chip Cell Cluster Growth and Cell Viability: The human ovarian carcinoma cell line SKOV3 (ATCC) was maintained and expanded following standard culture protocols as previously described.

Techniques: Fluorescence, Live Dead Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Long non-coding RNA KCNQ1OT1 promotes ovarian cancer cell malignant characteristics by targeting the miR-140-5p/KLK10 axis

doi: 10.3892/etm.2026.13129

Figure Lengend Snippet: Expression of target genes in OC datasets and cell lines. Principal component analysis plots showing expression patterns in the (A) GSE66957 and (C) GSE47841 datasets. Volcano plots illustrating differentially expressed genes in the (B) GSE66957 and (D) GSE47841 datasets. Differential expression of (E) KCNQ1OT1 ( GSE66957 ), (F) KLK10 ( GSE66957 ) and (G) miR-140-5p ( GSE47841 ). (H) Relative expression levels of KCNQ1OT1, KLK10 and miR-140-5p in SKOV3 OC cells and IOSE80 normal ovarian epithelial cells. * P<0.05 and ** P<0.01. OC, ovarian cancer; KCNQ1OT1, potassium voltage-gated channel subfamily Q member 1 opposite strand/antisense transcript 1; KLK10, kallikrein-related peptidase 10; miR, microRNA; Dim, dimension; FC, fold change.

Article Snippet: IOSE80 (normal ovarian epithelial) and SKOV3 (OC) cell lines were sourced from Procell Life Science & Technology Co., Ltd. IOSE80 cells were maintained in DMEM (Gibco; Thermo Fisher Scientific, Inc.) and SKOV3 cells in McCoy's 5A medium (Gibco; Thermo Fisher Scientific, Inc.), both supplemented with 10% FBS (HyClone; Cytiva) and 1% penicillin-streptomycin, at 37 ̊C in a 5% CO 2 humidified incubator.

Techniques: Expressing, Quantitative Proteomics