Journal: Scientific Reports

Article Title: Targeted therapy of pyrrolo[2,3- d ]pyrimidine antifolates in a syngeneic mouse model of high grade serous ovarian cancer and the impact on the tumor microenvironment

doi: 10.1038/s41598-022-14788-5

Figure Lengend Snippet: Expression of GARFTase and AICARFTase transcripts in primary EOC patient samples and BR-5 and BR-Luc cells. Transcript levels for GARFTase ( A ) and AICARFTase ( B ) were measured using cDNAs from primary specimens including normal ovary (n = 8) and HGSOC (n = 39) (OriGene) and results were compared to those for EOC cell lines including IGROV1, SKOV3, A2780 and A2780 E-80. Transcript levels were normalized to β-actin transcripts. Statistical analyses were performed between normal samples/tissues and tumor samples/tissues using the Wilcoxon rank-sum test. ( C , D ) Transcript levels of cytosolic C1 metabolic targets (GARFTase and AICARFTase) were determined in the BR-5 and BR-Luc syngeneic mouse models of HGSOC by real-time RT-PCR. Transcripts were normalized to β-actin transcripts and results are shown relative to levels in mouse liver (assigned a value of 1). Results are presented as mean values ± standard errors from at least three experiments. The p values are as follows: **** p < 0.0001. See Supplementary Table for patient characteristics and pathology.

Article Snippet: Transcript levels for GARFTase ( A ) and AICARFTase ( B ) were measured using cDNAs from primary specimens including normal ovary (n = 8) and HGSOC (n = 39) (OriGene) and results were compared to those for EOC cell lines including IGROV1, SKOV3, A2780 and A2780 E-80.

Techniques: Expressing, Quantitative RT-PCR

Journal: International Journal of Gynecological Cancer

Article Title: Docosahexaenoic Acid Modulates Invasion and Metastasis of Human Ovarian Cancer via Multiple Molecular Pathways

doi: 10.1097/IGC.0000000000000746

Figure Lengend Snippet: The cytotoxicity of ALA and DHA in A2780, HO8910, and SKOV-3. The effects of ALA and DHA on cell viability in A2780, HO8910, and SKOV-3 were determined by CCK-8 assay. Each column shows cell viability (%) mean ± SD of 3 independent experiments performed in triplicate. ALA was used as isotype control.

Article Snippet: The human ovarian cancer cell lines A2780, HO8910, and SKOV-3 were purchased from Enogene Biotech Co, Ltd (Jiangsu, China).

Techniques: CCK-8 Assay

Journal: International Journal of Gynecological Cancer

Article Title: Docosahexaenoic Acid Modulates Invasion and Metastasis of Human Ovarian Cancer via Multiple Molecular Pathways

doi: 10.1097/IGC.0000000000000746

Figure Lengend Snippet: The effect of ALA and DHA on invasion abilities in A2780, HO8910, and SKOV-3 cells. A2780, HO8910, and SKOV-3 cells were pretreated with ALA (64 μM, 120 μM, and 60 μM), DHA (32/64 μM, 60/120 μM, and 30/60 μM), or without DHA for 72 hours, respectively. Images of A2780 were acquired at 200 magnification, and images of HO8910 and SKOV-3 were acquired at 100 magnification under an inverted microscope. Each column shows the mean of 3 independent experiments performed in triplicate. The asterisks labeled above the error bars indicated significant differences compared with the group treated with ALA or 0.1% DMSO (*** P < 0.005 compared with the control). ALA was used as isotype control.

Article Snippet: The human ovarian cancer cell lines A2780, HO8910, and SKOV-3 were purchased from Enogene Biotech Co, Ltd (Jiangsu, China).

Techniques: Inverted Microscopy, Labeling

Journal: International Journal of Gynecological Cancer

Article Title: Docosahexaenoic Acid Modulates Invasion and Metastasis of Human Ovarian Cancer via Multiple Molecular Pathways

doi: 10.1097/IGC.0000000000000746

Figure Lengend Snippet: The effect of DHA on expression of genes or proteins associated with invasion and metastasis (A) A2780, HO8910, and SKOV-3 cells were respectively pretreated with (64 μM, 120 μM, and 60 μM) or without DHA for 72 hours. RT-PCR analysis was used to assess the relative expression of KISS-1, VEGF, MMP-9, TIMP-1, WAVE3, and PPAR-γ. The fold change of gene expression was calculated by the 2-ΔΔCT method. GAPDH was used as the reference gene. Cells with ALA treatment were used as the respective control (*** P < 0.005, ** P < 0.01, * P < 0.05). B, A2780, HO8910, and SKOV-3 cells were pretreated with DHA (32 and 64 μM, 60 and 120 μM, 30 and 60 μM) for 72 hours, respectively. Western blot analysis was performed using antibodies against NF-κB, VEGF, MMP-9, MMP-3, STAT3, WAVE3, COX-2, and N-Ca. The expressions of some genes or proteins were not detected by RT-PCR or Western bolt (the blanks were shown).

Article Snippet: The human ovarian cancer cell lines A2780, HO8910, and SKOV-3 were purchased from Enogene Biotech Co, Ltd (Jiangsu, China).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot