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sir-actin  (Spirochrome)


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    Structured Review

    Spirochrome sir-actin
    Sir Actin, supplied by Spirochrome, used in various techniques. Bioz Stars score: 97/100, based on 1035 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sir+actin/SiR-actin/custom%40sc001%4010%2E64898%2F2026%2E08%2E24%2E746798
    Average 97 stars, based on 1035 article reviews
    sir-actin - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Live Cell Imaging:

    Article Title: JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy.
    Article Snippet: Slides or plates were imaged using a Nikon Eclipse Ti inverted microscope equipped with an Andor Zyla4.2 sCMOS camera and using a Plan Apo λ 20x/0.75 objective lens or a Zeiss Axio Observer.Z1/7 microscope LSM800 with a PlanApochromat 20x/0.80 objective lens. .. For live cell imaging, cells were stained with SiR-actin (Spirochrome, SC001) or SPY-tubulin (Spirochrome, SC503) and imaged with a Nikon Eclipse Ti2-E inverted microscope equipped with an Okolab incubation chamber or Nikon BioStation IM-Q time-lapse imaging system with a 40x/ 0.80 objective lens. .. Whole images were quantified for aggregates within cardiomyocytes or F-actin-positive cells with NIS Elements software or ImageJ by setting a threshold for each channel, using the default method, and defining the cardiomyocyte area as a region of interest (ROI).

    Article Title: Cell loss disrupts mechanical homeostasis to drive retinal pigment epithelium ageing-like phenotype in vitro.
    Article Snippet: Myosin II inhibition was performed using 5 μM blebbistatin (Abcam, ab120425) for 1 h before nanoindentation and for 30min before POS addition in blebbistatin-containing medium (see above). .. Extrusion events were quantified from live cell imaging of apoptosis induction on SiR-Actin (Spirochrome, SC001) labelled cells. ..

    Article Title: Oncogenic Ras-Src-cortactin signaling rewires actin-generated forces to drive basement membrane rupture and initiate breast cancer invasion
    Article Snippet: .. For live cell imaging, spheroids were stained in assay medium with a final concentration of 10 nM SiR-actin (Spirochrome, SC001, solved in DMSO, final DMSO concentration = 1×10 -5 %) for 2.5 hours, isolated from EHS matrix and incubated with 1:100 Alexa Fluor 488-conjugated anti-laminin-5 antibody (Sigma-Aldrich, MAB19562X; clone D4B5) for 1 hour at 4 °C. ..

    Staining:

    Article Title: JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy.
    Article Snippet: Slides or plates were imaged using a Nikon Eclipse Ti inverted microscope equipped with an Andor Zyla4.2 sCMOS camera and using a Plan Apo λ 20x/0.75 objective lens or a Zeiss Axio Observer.Z1/7 microscope LSM800 with a PlanApochromat 20x/0.80 objective lens. .. For live cell imaging, cells were stained with SiR-actin (Spirochrome, SC001) or SPY-tubulin (Spirochrome, SC503) and imaged with a Nikon Eclipse Ti2-E inverted microscope equipped with an Okolab incubation chamber or Nikon BioStation IM-Q time-lapse imaging system with a 40x/ 0.80 objective lens. .. Whole images were quantified for aggregates within cardiomyocytes or F-actin-positive cells with NIS Elements software or ImageJ by setting a threshold for each channel, using the default method, and defining the cardiomyocyte area as a region of interest (ROI).

    Article Title: Oncogenic Ras-Src-cortactin signaling rewires actin-generated forces to drive basement membrane rupture and initiate breast cancer invasion
    Article Snippet: .. For live cell imaging, spheroids were stained in assay medium with a final concentration of 10 nM SiR-actin (Spirochrome, SC001, solved in DMSO, final DMSO concentration = 1×10 -5 %) for 2.5 hours, isolated from EHS matrix and incubated with 1:100 Alexa Fluor 488-conjugated anti-laminin-5 antibody (Sigma-Aldrich, MAB19562X; clone D4B5) for 1 hour at 4 °C. ..

    Inverted Microscopy:

    Article Title: JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy.
    Article Snippet: Slides or plates were imaged using a Nikon Eclipse Ti inverted microscope equipped with an Andor Zyla4.2 sCMOS camera and using a Plan Apo λ 20x/0.75 objective lens or a Zeiss Axio Observer.Z1/7 microscope LSM800 with a PlanApochromat 20x/0.80 objective lens. .. For live cell imaging, cells were stained with SiR-actin (Spirochrome, SC001) or SPY-tubulin (Spirochrome, SC503) and imaged with a Nikon Eclipse Ti2-E inverted microscope equipped with an Okolab incubation chamber or Nikon BioStation IM-Q time-lapse imaging system with a 40x/ 0.80 objective lens. .. Whole images were quantified for aggregates within cardiomyocytes or F-actin-positive cells with NIS Elements software or ImageJ by setting a threshold for each channel, using the default method, and defining the cardiomyocyte area as a region of interest (ROI).

    Incubation:

    Article Title: JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy.
    Article Snippet: Slides or plates were imaged using a Nikon Eclipse Ti inverted microscope equipped with an Andor Zyla4.2 sCMOS camera and using a Plan Apo λ 20x/0.75 objective lens or a Zeiss Axio Observer.Z1/7 microscope LSM800 with a PlanApochromat 20x/0.80 objective lens. .. For live cell imaging, cells were stained with SiR-actin (Spirochrome, SC001) or SPY-tubulin (Spirochrome, SC503) and imaged with a Nikon Eclipse Ti2-E inverted microscope equipped with an Okolab incubation chamber or Nikon BioStation IM-Q time-lapse imaging system with a 40x/ 0.80 objective lens. .. Whole images were quantified for aggregates within cardiomyocytes or F-actin-positive cells with NIS Elements software or ImageJ by setting a threshold for each channel, using the default method, and defining the cardiomyocyte area as a region of interest (ROI).

    Article Title: STED/AFM as a tool to investigate mechanical and adhesive properties of migrating keratinocytes
    Article Snippet: .. Briefly, cells exposed to complete high-calcium medium were incubated for 1 h with SiR-Actin (1 μM; Spirochrome, Denver, USA). .. After staining, the cells were washed once more with medium and transferred into the BioCell holder (Bruker Nano, Berlin, Germany), pre-heated to 37°C, within the integrated STED/AFM setup.

    Article Title: STED/AFM as a tool to investigate mechanical and adhesive properties of migrating keratinocytes.
    Article Snippet: .. Briefly, cells exposed to complete high-calcium medium were incubated for 1 h with SiR-Actin (1 μM; Spirochrome, Denver, USA). .. After staining, the cells were washed once more with medium and transferred into the BioCell holder (Bruker Nano, Berlin, Germany), pre-heated to 37°C, within the integrated STED/ AFM setup.

    Article Title: Oncogenic Ras-Src-cortactin signaling rewires actin-generated forces to drive basement membrane rupture and initiate breast cancer invasion
    Article Snippet: .. For live cell imaging, spheroids were stained in assay medium with a final concentration of 10 nM SiR-actin (Spirochrome, SC001, solved in DMSO, final DMSO concentration = 1×10 -5 %) for 2.5 hours, isolated from EHS matrix and incubated with 1:100 Alexa Fluor 488-conjugated anti-laminin-5 antibody (Sigma-Aldrich, MAB19562X; clone D4B5) for 1 hour at 4 °C. ..

    Imaging:

    Article Title: JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy.
    Article Snippet: Slides or plates were imaged using a Nikon Eclipse Ti inverted microscope equipped with an Andor Zyla4.2 sCMOS camera and using a Plan Apo λ 20x/0.75 objective lens or a Zeiss Axio Observer.Z1/7 microscope LSM800 with a PlanApochromat 20x/0.80 objective lens. .. For live cell imaging, cells were stained with SiR-actin (Spirochrome, SC001) or SPY-tubulin (Spirochrome, SC503) and imaged with a Nikon Eclipse Ti2-E inverted microscope equipped with an Okolab incubation chamber or Nikon BioStation IM-Q time-lapse imaging system with a 40x/ 0.80 objective lens. .. Whole images were quantified for aggregates within cardiomyocytes or F-actin-positive cells with NIS Elements software or ImageJ by setting a threshold for each channel, using the default method, and defining the cardiomyocyte area as a region of interest (ROI).

    Article Title: Scaffold protein SHANK3 regulates endothelial cell motility and tissue mechanics
    Article Snippet: .. Cells were seeded onto hydrogels 24 h, and 2 h prior to imaging media was replaced with media +/−doxycycline containing 20 mM HEPES (pH 7.0-7.6, Sigma), 2 μg/ml Hoescht (Invitrogen), and 200 nM SiR-actin (Spirochrome) to enable visualisation of cells. .. For detection of traction forces exerted by the cells, beads were imaged before and after removal of cells with 15 μl pre-warmed 20% SDS, with a Marianas CSU-W1 Spinning disk spinning disk confocal microscope (3i/Zeiss) using a LD C-APOCHROMAT 40x/1.1 W M27 objective (Zeiss) and sCMOS Orca Flash 4.0 camera (Hamamatsu).

    Article Title: Spectrin coordinates cell shape and signaling essential for epidermal differentiation
    Article Snippet: .. At confluency, the medium was switched to a high Ca 2+ medium for 48 h. 2 h prior to imaging, the medium was changed to a high Ca 2+ medium containing 1 μM SiR-actin (#CY-SC001; Spirochrome). .. For laser ablation, a spinning Disk microscope (UltaView VoX, Perkin Elmer) equipped with a 355 nM pulsed YAG laser (Rapp Opto Electronic) and a Hamamatsu EMCCD C9100-50 camera was used with Volocity software.

    Concentration Assay:

    Article Title: Oncogenic Ras-Src-cortactin signaling rewires actin-generated forces to drive basement membrane rupture and initiate breast cancer invasion
    Article Snippet: .. For live cell imaging, spheroids were stained in assay medium with a final concentration of 10 nM SiR-actin (Spirochrome, SC001, solved in DMSO, final DMSO concentration = 1×10 -5 %) for 2.5 hours, isolated from EHS matrix and incubated with 1:100 Alexa Fluor 488-conjugated anti-laminin-5 antibody (Sigma-Aldrich, MAB19562X; clone D4B5) for 1 hour at 4 °C. ..

    Isolation:

    Article Title: Oncogenic Ras-Src-cortactin signaling rewires actin-generated forces to drive basement membrane rupture and initiate breast cancer invasion
    Article Snippet: .. For live cell imaging, spheroids were stained in assay medium with a final concentration of 10 nM SiR-actin (Spirochrome, SC001, solved in DMSO, final DMSO concentration = 1×10 -5 %) for 2.5 hours, isolated from EHS matrix and incubated with 1:100 Alexa Fluor 488-conjugated anti-laminin-5 antibody (Sigma-Aldrich, MAB19562X; clone D4B5) for 1 hour at 4 °C. ..



    Similar Products

    97
    Spirochrome sir-actin
    Sir Actin, supplied by Spirochrome, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sir+actin/SiR-actin/custom%40sc001%4010%2E64898%2F2026%2E08%2E24%2E746798
    Average 97 stars, based on 1 article reviews
    sir-actin - by Bioz Stars, 2026-09
    97/100 stars
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