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Hamamatsu simple pci software
Simple Pci Software, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simple+pci+software/simple+pci+software/pm39729988-518-6-8
Average 90 stars, based on 1 article reviews
simple pci software - by Bioz Stars, 2026-11
90/100 stars

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other:

Article Title: mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities
Article Snippet: For widefield photobleaching experiments, transfected HeLa S3 cells (CCL2 line; ATCC) in a Bioptechs Delta-T imaging chamber were imaged on a Nikon TE2000 inverted microscope equipped with a 40× dry objective (Nikon Plan Fluorite NA = 0.85) and an X-Cite exacte light source (Lumen Dynamics).


Software:

Article Title: A novel pathogenic variant c.262delA in PBX1 causing oligomeganephronia identified using whole-exome sequencing and a literature review.
Article Snippet: Correspondence Xiaowen Wang, Department of Nephrology, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.. Email: xiaowenwang331@163.com Abstract Oligomeganephronia (OMN) is a rare congenital renal hypoplasia reported more often in children than in adults.. The diagnosis of OMN relies on renal biopsy and exhibits a significant reduction in the number of glomeruli and pronounced glomerular hypertrophy.

Article Title: Post-Stroke Environmental Enrichment Improves Neurogenesis and Cognitive Function and Reduces the Generation of Aberrant Neurons in the Mouse Hippocampus
Article Snippet: .. Every 12th brain section was placed on a light table (Northern Light Precision Illuminator, MCID, Washington, DC, USA) and imaged with a digital camera (Hamamatsu Photonics K.K., Wexford Bayne, PA, USA) using Simple PCI software (version 6, Hamamatsu Photonics K.K., Wexford Bayne, PA, USA). .. Subsequently, brain and infarct areas were determined using Scion Image software (version 4, Scion Corporation, Torrance, CA, USA).

Article Title: CD103 + cDC1 Dendritic Cell Vaccine Therapy for Osteosarcoma Lung Metastases
Article Snippet: Tumor areas from each section were imaged at 400× by Leica DMi8 microscope (Leica Microsystems, Wetzlar, Germany). .. Positive staining was quantified in eight random microscopy fields from three tumor samples using Simple PCI software (Hamamatsu, Sewickley, PA, USA), and the average expression was calculated as average ± SEM. ..

Article Title: Post-Stroke Environmental Enrichment Improves Neurogenesis and Cognitive Function and Reduces the Generation of Aberrant Neurons in the Mouse Hippocampus.
Article Snippet: .. Every 12th brain section was placed on a light table (Northern Light Precision Illuminator, MCID, Washington, DC, USA) and imaged with a digital camera (Hamamatsu Photonics K.K., Wexford Bayne, PA, USA) using Simple PCI software (version 6, Hamamatsu Photonics K.K., Wexford Bayne, PA, USA). .. Subsequently, brain and infarct areas were determined using Scion Image software (version 4, Scion Corporation, Torrance, CA, USA).

Article Title: CD103 + cDC1 Dendritic Cell Vaccine Therapy for Osteosarcoma Lung Metastases
Article Snippet: Tumor areas from each section were imaged at 400× by Leica DMi8 microscope (Leica Mi-crosystems, Wetzlar, Germany). .. Positive staining was quantified in eight random microscopy fields from three tumor samples using Simple PCI software (version 6.6, Hamamatsu, Sewickley, PA, USA), and the average expression was calculated as average ± SEM. .. Primary antibodies anti-CD86 (1:200) and anti-CD163 (1:200) (CD86, NeoBiotechnologies, Union City, CA, USA; CD163, Abcam, Waltham, MA, USA) were used for the macrophage markers.

Article Title: Trans-generational epigenetic regulation of C. elegans primordial germ cells
Article Snippet: Secondary antibodies used were Alexa Fluor 488-conjugated goat anti-mouse and Alexa Fluor 594-conjugated anti-rabbit antibodies (1:500) (Molecular Probes, Eugene, OR, USA). .. Samples were mounted in anti-fade reagent (ProLong Gold; Molecular Probes) and observed under a fluorescence microscope (Leica DMRXA; Hamamatsu Photonics, Hamamatsu, Japan) with Simple PCI software (Hamamatsu Photonics). .. Primary antibodies used were: affinity-purified rabbit anti-MES-4 [ ] (1:10), mouse monoclonal CMA333 to H3K36me3 (0.25 μg/ml, rabbit anti-PGL-1 [ ] (1:10,000), mouse monoclonal OIC1D4 to P-granules [ ] (1:4), mouse monoclonal H5 to hyper-phosphorylated Pol II CTD (1:50) [ ] and mouse monoclonal 8WG16 to Pol II (Covance) (1:100).

Northern Blot:

Article Title: Post-Stroke Environmental Enrichment Improves Neurogenesis and Cognitive Function and Reduces the Generation of Aberrant Neurons in the Mouse Hippocampus
Article Snippet: .. Every 12th brain section was placed on a light table (Northern Light Precision Illuminator, MCID, Washington, DC, USA) and imaged with a digital camera (Hamamatsu Photonics K.K., Wexford Bayne, PA, USA) using Simple PCI software (version 6, Hamamatsu Photonics K.K., Wexford Bayne, PA, USA). .. Subsequently, brain and infarct areas were determined using Scion Image software (version 4, Scion Corporation, Torrance, CA, USA).

Article Title: Post-Stroke Environmental Enrichment Improves Neurogenesis and Cognitive Function and Reduces the Generation of Aberrant Neurons in the Mouse Hippocampus.
Article Snippet: .. Every 12th brain section was placed on a light table (Northern Light Precision Illuminator, MCID, Washington, DC, USA) and imaged with a digital camera (Hamamatsu Photonics K.K., Wexford Bayne, PA, USA) using Simple PCI software (version 6, Hamamatsu Photonics K.K., Wexford Bayne, PA, USA). .. Subsequently, brain and infarct areas were determined using Scion Image software (version 4, Scion Corporation, Torrance, CA, USA).

Staining:

Article Title: CD103 + cDC1 Dendritic Cell Vaccine Therapy for Osteosarcoma Lung Metastases
Article Snippet: Tumor areas from each section were imaged at 400× by Leica DMi8 microscope (Leica Microsystems, Wetzlar, Germany). .. Positive staining was quantified in eight random microscopy fields from three tumor samples using Simple PCI software (Hamamatsu, Sewickley, PA, USA), and the average expression was calculated as average ± SEM. ..

Article Title: CD103 + cDC1 Dendritic Cell Vaccine Therapy for Osteosarcoma Lung Metastases
Article Snippet: Tumor areas from each section were imaged at 400× by Leica DMi8 microscope (Leica Mi-crosystems, Wetzlar, Germany). .. Positive staining was quantified in eight random microscopy fields from three tumor samples using Simple PCI software (version 6.6, Hamamatsu, Sewickley, PA, USA), and the average expression was calculated as average ± SEM. .. Primary antibodies anti-CD86 (1:200) and anti-CD163 (1:200) (CD86, NeoBiotechnologies, Union City, CA, USA; CD163, Abcam, Waltham, MA, USA) were used for the macrophage markers.

Microscopy:

Article Title: CD103 + cDC1 Dendritic Cell Vaccine Therapy for Osteosarcoma Lung Metastases
Article Snippet: Tumor areas from each section were imaged at 400× by Leica DMi8 microscope (Leica Microsystems, Wetzlar, Germany). .. Positive staining was quantified in eight random microscopy fields from three tumor samples using Simple PCI software (Hamamatsu, Sewickley, PA, USA), and the average expression was calculated as average ± SEM. ..

Article Title: CD103 + cDC1 Dendritic Cell Vaccine Therapy for Osteosarcoma Lung Metastases
Article Snippet: Tumor areas from each section were imaged at 400× by Leica DMi8 microscope (Leica Mi-crosystems, Wetzlar, Germany). .. Positive staining was quantified in eight random microscopy fields from three tumor samples using Simple PCI software (version 6.6, Hamamatsu, Sewickley, PA, USA), and the average expression was calculated as average ± SEM. .. Primary antibodies anti-CD86 (1:200) and anti-CD163 (1:200) (CD86, NeoBiotechnologies, Union City, CA, USA; CD163, Abcam, Waltham, MA, USA) were used for the macrophage markers.

Article Title: Trans-generational epigenetic regulation of C. elegans primordial germ cells
Article Snippet: Secondary antibodies used were Alexa Fluor 488-conjugated goat anti-mouse and Alexa Fluor 594-conjugated anti-rabbit antibodies (1:500) (Molecular Probes, Eugene, OR, USA). .. Samples were mounted in anti-fade reagent (ProLong Gold; Molecular Probes) and observed under a fluorescence microscope (Leica DMRXA; Hamamatsu Photonics, Hamamatsu, Japan) with Simple PCI software (Hamamatsu Photonics). .. Primary antibodies used were: affinity-purified rabbit anti-MES-4 [ ] (1:10), mouse monoclonal CMA333 to H3K36me3 (0.25 μg/ml, rabbit anti-PGL-1 [ ] (1:10,000), mouse monoclonal OIC1D4 to P-granules [ ] (1:4), mouse monoclonal H5 to hyper-phosphorylated Pol II CTD (1:50) [ ] and mouse monoclonal 8WG16 to Pol II (Covance) (1:100).

Expressing:

Article Title: CD103 + cDC1 Dendritic Cell Vaccine Therapy for Osteosarcoma Lung Metastases
Article Snippet: Tumor areas from each section were imaged at 400× by Leica DMi8 microscope (Leica Microsystems, Wetzlar, Germany). .. Positive staining was quantified in eight random microscopy fields from three tumor samples using Simple PCI software (Hamamatsu, Sewickley, PA, USA), and the average expression was calculated as average ± SEM. ..

Article Title: CD103 + cDC1 Dendritic Cell Vaccine Therapy for Osteosarcoma Lung Metastases
Article Snippet: Tumor areas from each section were imaged at 400× by Leica DMi8 microscope (Leica Mi-crosystems, Wetzlar, Germany). .. Positive staining was quantified in eight random microscopy fields from three tumor samples using Simple PCI software (version 6.6, Hamamatsu, Sewickley, PA, USA), and the average expression was calculated as average ± SEM. .. Primary antibodies anti-CD86 (1:200) and anti-CD163 (1:200) (CD86, NeoBiotechnologies, Union City, CA, USA; CD163, Abcam, Waltham, MA, USA) were used for the macrophage markers.

Fluorescence:

Article Title: Trans-generational epigenetic regulation of C. elegans primordial germ cells
Article Snippet: Secondary antibodies used were Alexa Fluor 488-conjugated goat anti-mouse and Alexa Fluor 594-conjugated anti-rabbit antibodies (1:500) (Molecular Probes, Eugene, OR, USA). .. Samples were mounted in anti-fade reagent (ProLong Gold; Molecular Probes) and observed under a fluorescence microscope (Leica DMRXA; Hamamatsu Photonics, Hamamatsu, Japan) with Simple PCI software (Hamamatsu Photonics). .. Primary antibodies used were: affinity-purified rabbit anti-MES-4 [ ] (1:10), mouse monoclonal CMA333 to H3K36me3 (0.25 μg/ml, rabbit anti-PGL-1 [ ] (1:10,000), mouse monoclonal OIC1D4 to P-granules [ ] (1:4), mouse monoclonal H5 to hyper-phosphorylated Pol II CTD (1:50) [ ] and mouse monoclonal 8WG16 to Pol II (Covance) (1:100).



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Hamamatsu simple pci imaging software
Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple <t>PCI</t> image <t>analysis</t> <t>software</t> (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.
Simple Pci Imaging Software, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simple+pci+software/simple+pci+imaging+software/pmc10411948-99-8-10
Average 90 stars, based on 1 article reviews
simple pci imaging software - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

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Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple PCI image analysis software (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.

Journal: Fertility and sterility

Article Title: Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling

doi: 10.1016/j.fertnstert.2015.02.025

Figure Lengend Snippet: Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple PCI image analysis software (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.

Article Snippet: Images were processed to determine gray level using Simple PCI (Hamamatsu) imaging software, as previously described ( 31 , 32 ).

Techniques: Cell Culture, Labeling, Software