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non-linear regression fit (sigmoidal, 4pl) function  (GraphPad Software Inc)


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    GraphPad Software Inc non-linear regression fit (sigmoidal, 4pl) function
    Differential scanning fluorimetry and cytotoxicity results for pre-miR-21 stability modulating compounds. PubChem CID # is the compound’s identifier ( https://pubchem.ncbi.nlm.nih.gov/ ). The Δ T m MAX was calculated by taking the melting temperature shift difference of pre-miR-21 treated with the compound vs untreated. a The apparent Kd ( K d app ) was measured by direct binding assay using intrinsic fluorescence max 545/580 nm. b GI 50 was determined using the XTT cell viability assay. Calculations were performed using the <t> non-linear regression fit </t> (Sigmoidal, <t> 4PL) </t> function in GraphPad Prism. cNormal colon cells used were ATCC CRL-1790. d 1,5-bis[3-(2-hydroxyethylamino)propylamino]anthracene-9,10-dione hydrochloride. All experiments were repeated in triplicate.
    Non Linear Regression Fit (Sigmoidal, 4pl) Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigmoidal+non-linear+fit+function/non+linear+regression+analysis/pmc06697619-154-6-13
    Average 90 stars, based on 1 article reviews
    non-linear regression fit (sigmoidal, 4pl) function - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "The Natural Product Butylcycloheptyl Prodiginine Binds Pre-miR-21, Inhibits Dicer-Mediated Processing of Pre-miR-21, and Blocks Cellular Proliferation"

    Article Title: The Natural Product Butylcycloheptyl Prodiginine Binds Pre-miR-21, Inhibits Dicer-Mediated Processing of Pre-miR-21, and Blocks Cellular Proliferation

    Journal: Cell chemical biology

    doi: 10.1016/j.chembiol.2019.04.011

    Differential scanning fluorimetry and cytotoxicity results for pre-miR-21 stability modulating compounds. PubChem CID # is the compound’s identifier ( https://pubchem.ncbi.nlm.nih.gov/ ). The Δ T m MAX was calculated by taking the melting temperature shift difference of pre-miR-21 treated with the compound vs untreated. a The apparent Kd ( K d app ) was measured by direct binding assay using intrinsic fluorescence max 545/580 nm. b GI 50 was determined using the XTT cell viability assay. Calculations were performed using the non-linear regression fit (Sigmoidal, 4PL) function in GraphPad Prism. cNormal colon cells used were ATCC CRL-1790. d 1,5-bis[3-(2-hydroxyethylamino)propylamino]anthracene-9,10-dione hydrochloride. All experiments were repeated in triplicate.
    Figure Legend Snippet: Differential scanning fluorimetry and cytotoxicity results for pre-miR-21 stability modulating compounds. PubChem CID # is the compound’s identifier ( https://pubchem.ncbi.nlm.nih.gov/ ). The Δ T m MAX was calculated by taking the melting temperature shift difference of pre-miR-21 treated with the compound vs untreated. a The apparent Kd ( K d app ) was measured by direct binding assay using intrinsic fluorescence max 545/580 nm. b GI 50 was determined using the XTT cell viability assay. Calculations were performed using the non-linear regression fit (Sigmoidal, 4PL) function in GraphPad Prism. cNormal colon cells used were ATCC CRL-1790. d 1,5-bis[3-(2-hydroxyethylamino)propylamino]anthracene-9,10-dione hydrochloride. All experiments were repeated in triplicate.

    Techniques Used: Binding Assay, Fluorescence, Viability Assay

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    Article Title: Expression of the Arabidopsis thaliana immune receptor EFR in Medicago truncatula reduces infection by a root pathogenic bacterium, but not nitrogen‐fixing rhizobial symbiosis
    Article Snippet: Orange line was calculated by the sigmoidal non-linear fit function in GraphPad Prism 5.



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    Differential scanning fluorimetry and cytotoxicity results for pre-miR-21 stability modulating compounds. PubChem CID # is the compound’s identifier ( https://pubchem.ncbi.nlm.nih.gov/ ). The Δ T m MAX was calculated by taking the melting temperature shift difference of pre-miR-21 treated with the compound vs untreated. a The apparent Kd ( K d app ) was measured by direct binding assay using intrinsic fluorescence max 545/580 nm. b GI 50 was determined using the XTT cell viability assay. Calculations were performed using the non-linear regression fit (Sigmoidal, 4PL) function in GraphPad Prism. cNormal colon cells used were ATCC CRL-1790. d 1,5-bis[3-(2-hydroxyethylamino)propylamino]anthracene-9,10-dione hydrochloride. All experiments were repeated in triplicate.

    Journal: Cell chemical biology

    Article Title: The Natural Product Butylcycloheptyl Prodiginine Binds Pre-miR-21, Inhibits Dicer-Mediated Processing of Pre-miR-21, and Blocks Cellular Proliferation

    doi: 10.1016/j.chembiol.2019.04.011

    Figure Lengend Snippet: Differential scanning fluorimetry and cytotoxicity results for pre-miR-21 stability modulating compounds. PubChem CID # is the compound’s identifier ( https://pubchem.ncbi.nlm.nih.gov/ ). The Δ T m MAX was calculated by taking the melting temperature shift difference of pre-miR-21 treated with the compound vs untreated. a The apparent Kd ( K d app ) was measured by direct binding assay using intrinsic fluorescence max 545/580 nm. b GI 50 was determined using the XTT cell viability assay. Calculations were performed using the non-linear regression fit (Sigmoidal, 4PL) function in GraphPad Prism. cNormal colon cells used were ATCC CRL-1790. d 1,5-bis[3-(2-hydroxyethylamino)propylamino]anthracene-9,10-dione hydrochloride. All experiments were repeated in triplicate.

    Article Snippet: T m was obtained using the non-linear regression fit (Sigmoidal, 4PL) function in GraphPad Prism where y = fluorescence, x = temperature. ( C ) a concentration of 0, 12.5, 25, 50, and 100 nM of 1 was incubated with buffer (black), 50 ng/μL purified total RNA from HCT-116 cells (red), 25 ng/μL purified genomic DNA from HCT-116 cells (green), or 50 ng/μL of plasmid DNA (blue).

    Techniques: Binding Assay, Fluorescence, Viability Assay