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sigmoidal dose–response function  (GraphPad Software Inc)


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    GraphPad Software Inc sigmoidal dose–response function
    Sigmoidal Dose–Response Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigmoidal+dose-response/sigmoidal+dose+response+curve/pm40649812-313-9-12
    Average 90 stars, based on 1 article reviews
    sigmoidal dose–response function - by Bioz Stars, 2026-10
    90/100 stars

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    Article Snippet: Sortase A (SrtA) anchors surface proteins to the cell wall envelope, and it has attracted increasing interesting as a potential antivirulence target.. Several small-molecule inhibitors for SrtA have been developed, but target validation remains largely underexplored.. Herein, we report a new class of SrtA inhibitors that supports antivirulence therapy through small-molecule targeting of SrtA.

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    Article Snippet: Data was fitted to a sigmoidal dose-response with GraphPad Prism.

    Article Title: Evaluation of new indole and bromoindole derivatives as pp60(c-Src) tyrosine kinase inhibitors.
    Article Snippet: A series of N-benzyl-indole-3-imine-, amine derivatives and their 5-bromo congeners were synthesized and their biological activity were evaluated against the pp60 tyrosine kinase target.. To afford the imine derivatives, aldehydes were reacted with substituted benzylamines and the corresponding amine derivatives were obtained by NaBH4 reduction of these imines.. Except insoluble N-benzyl-indole-3-imine derivatives, all the derivatives showed some activity against the kinase target.

    Article Title: Molecular insights into ligand recognition and activation of chemokine receptors CCR2 and CCR3
    Article Snippet: Nonlinear regression analysis was performed using a sigmoidal dose-response in GraphPad Prism to calculate the values of E max and EC 50 .

    Article Title: Synthesis and pp60c-Src tyrosine kinase inhibitory activities of novel indole-3-imine and amine derivatives substituted at N1 and C5.
    Article Snippet: A series of novel 1,3,5-trisubstituted indole derivatives, namely, N-benzyl 5-phenyl indole-3imine, N-benzyl-5-(p-fluorophenyl)indole-3-imine and their corresponding amine congeners, were designed and synthesized as pp60 tyrosine kinase inhibitors, and their inhibitory activities toward pp60 tyrosine kinase were evaluated by in-vitro kinase assay.. Pre-screening at two doses of compounds against kinase target revealed that, except for the N-benzyl-5-phenyl indole imine derivatives 7a–7d, all indole derivatives show the target inhibition at varying levels.. Consequently, the compounds, 8c, 8f, 8g, and 8h, were selected for prescreening tests.

    Article Title: Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation
    Article Snippet: Data was fitted to a sigmoidal dose-response with GraphPad Prism and can be found in the Source Data file.

    Article Title: Efflux Attenuates the Antibacterial Activity of Q203 in Mycobacterium tuberculosis
    Article Snippet: IC50 values were calculated based on the 347 on A pril 23, 2017 by H A C E T T E P E U N IV E R S IT Y http://aac.asm .org/ D ow nloaded from 16 concentrations required to inhibit bacterial growth by 50% by fitting the curves with a 348 sigmoidal dose-response using GraphPad Prism software (Version 6.05).

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    Article Snippet: Data were fitted using the sigmoidal dose-response in Prism (GraphPad).



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    GraphPad Software Inc nonlinear regression curve fitting package (sigmoidal dose–response)
    IC 50 and IC 90 values (μM) of anti-tumor drugs for monolayer and spheroid-cultured cells in a 96-well format (Fig. <xref ref-type= S2 , n = 3). Each cell culture was performed at 37 °C in an atmosphere containing 5% CO 2 , and treated with various concentrations of drugs for 48 h. Cell viability was assessed using the MTS assay reagent (Promega). Data were analyzed using a nonlinear regression curve-fitting package in Prism 4 (GraphPad Software Inc.) (Fig. S2 ). The drugs tested included: docetaxel, a mitotic inhibitor; irinotecan, a topoisomerase I inhibitor; 5-fluorouracil (5-FU), a thymidylate synthase inhibitor; sunitinib, a multi-receptor tyrosine kinase (RTK) inhibitor; tirapazamine, a hypoxia-activated prodrug; and FX11, a lactate dehydrogenase inhibitor. Radial organization of each cell on the spheroidal culture dish (IWAKI) conferred resistance to the most of drugs tested." width="250" height="auto" />
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    S2 , n = 3). Each cell culture was performed at 37 °C in an atmosphere containing 5% CO 2 , and treated with various concentrations of drugs for 48 h. Cell viability was assessed using the MTS assay reagent (Promega). Data were analyzed using a nonlinear regression curve-fitting package in Prism 4 (GraphPad Software Inc.) (Fig. S2 ). The drugs tested included: docetaxel, a mitotic inhibitor; irinotecan, a topoisomerase I inhibitor; 5-fluorouracil (5-FU), a thymidylate synthase inhibitor; sunitinib, a multi-receptor tyrosine kinase (RTK) inhibitor; tirapazamine, a hypoxia-activated prodrug; and FX11, a lactate dehydrogenase inhibitor. Radial organization of each cell on the spheroidal culture dish (IWAKI) conferred resistance to the most of drugs tested." width="100%" height="100%">

    Journal: Scientific Reports

    Article Title: Hyperpolarized [1- 13 C]pyruvate NMR spectroscopy reveals transition of tumor energy metabolism in microscale multicellular spheroids

    doi: 10.1038/s41598-025-03454-1

    Figure Lengend Snippet: IC 50 and IC 90 values (μM) of anti-tumor drugs for monolayer and spheroid-cultured cells in a 96-well format (Fig. S2 , n = 3). Each cell culture was performed at 37 °C in an atmosphere containing 5% CO 2 , and treated with various concentrations of drugs for 48 h. Cell viability was assessed using the MTS assay reagent (Promega). Data were analyzed using a nonlinear regression curve-fitting package in Prism 4 (GraphPad Software Inc.) (Fig. S2 ). The drugs tested included: docetaxel, a mitotic inhibitor; irinotecan, a topoisomerase I inhibitor; 5-fluorouracil (5-FU), a thymidylate synthase inhibitor; sunitinib, a multi-receptor tyrosine kinase (RTK) inhibitor; tirapazamine, a hypoxia-activated prodrug; and FX11, a lactate dehydrogenase inhibitor. Radial organization of each cell on the spheroidal culture dish (IWAKI) conferred resistance to the most of drugs tested.

    Article Snippet: Cell viability in each drug concentration was plotted (Fig. ), and the sigmoidal dose–response curve was calculated using a nonlinear regression curve fitting package (sigmoidal dose–response) in Prism 4 (GraphPad Software Inc., La Jolla, CA).

    Techniques: Cell Culture, MTS Assay, Software