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shspred1 gfp tl301404 lentiviral vectors  (OriGene)


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    Structured Review

    OriGene shspred1 gfp tl301404 lentiviral vectors
    Shspred1 Gfp Tl301404 Lentiviral Vectors, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+plasmids/SPRED1+Human+shRNA+Plasmid+Kit/pm42840422-227-78-85
    Average 94 stars, based on 1 article reviews
    shspred1 gfp tl301404 lentiviral vectors - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Over Expression:

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation
    Article Snippet: .. Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation.
    Article Snippet: .. Lentivirus production and cell infection Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Produced:

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation
    Article Snippet: .. Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation.
    Article Snippet: .. Lentivirus production and cell infection Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Construct:

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation
    Article Snippet: .. Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation.
    Article Snippet: .. Lentivirus production and cell infection Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Knockdown:

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation
    Article Snippet: .. Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation.
    Article Snippet: .. Lentivirus production and cell infection Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Article Title: Immunomodulatory Properties of Polysaccharides from the Coral Pseudopterogorgia americana in Macrophages
    Article Snippet: .. Stable PKC-α and PKC-δ knockdown RAW264.7 and mock cells were obtained from stable transfection with shRNA plasmids that target PKC-α (catalogue number: TG501653), PKC-δ (catalogue number: TR513052) and a scrambled sequence (catalogue number: TR30012), respectively, which were obtained from OriGene Technologies, Inc. (Rockville, MD, USA). .. The RAW-Blue cell line is a RAW264.7 cell-based NF-κB reporter cell line that was obtained from InvivoGen (San Diego, CA, USA).

    shRNA:

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation
    Article Snippet: .. Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Article Title: CREG1 promotes lysosomal biogenesis and function.
    Article Snippet: .. For the expression of the CREG1-GFP fusion protein and tag-less CREG1, the HsCREG1 cDNA was subcloned in frame from pcDNA3.1 to pEGFP-N3 and pLPCX-IRESGPF, respectively. pCMV6-CREG1, pCMV6, and the shRNA plasmids targeting mouse Creg1 as well as the scrambled control were purchased from Origene Technologies (RC201654, PS100001, and TF510293, respectively). ..

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation.
    Article Snippet: .. Lentivirus production and cell infection Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Article Title: Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPARγ binodal complementary modules
    Article Snippet: Cells were incubated with the 5 μM CellRox Deep Red Reagent (Invitrogen) at 37 °C for 30 min and Hoescht for 20 min, washed and imaged using a confocal microscope at excitation/emission maxima for the reagent 644/665 nm. .. shRNA plasmids, including a non-target control (#TR30013) and several shRNA targeting ASK1 (FI37885, #FI37886, #FI37887, #FI37888; Origene) were transfected using Lipofectamine 2000 (Invitrogen). ..

    Article Title: Immunomodulatory Properties of Polysaccharides from the Coral Pseudopterogorgia americana in Macrophages
    Article Snippet: .. Stable PKC-α and PKC-δ knockdown RAW264.7 and mock cells were obtained from stable transfection with shRNA plasmids that target PKC-α (catalogue number: TG501653), PKC-δ (catalogue number: TR513052) and a scrambled sequence (catalogue number: TR30012), respectively, which were obtained from OriGene Technologies, Inc. (Rockville, MD, USA). .. The RAW-Blue cell line is a RAW264.7 cell-based NF-κB reporter cell line that was obtained from InvivoGen (San Diego, CA, USA).

    Article Title: Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage
    Article Snippet: RFP-Rab5 140 and 180 mutants were generated with primers (5′-GCAGCAGCTGTTGACTTCCAGG-3′ and 5′-ATTTGCTAAGTCAGCTTTGTTTCCTGAC-3′) and (5′-GCAGCGCTGCCAAAGAATGAAC-3′ and 5′-AGCTATTGCCATAAATATTTCATTTACATTCATTG-3′), respectively, using a KOD-plus mutagenesis kit (Toyobo, Osaka, Japan). .. NIH3T3 and HeLa cells were incubated in DMEM containing 10% FBS, 100 units of penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified atmosphere with 5% CO 2 . shRNA plasmids targeting human or mouse PARP1 or scrambled shRNA (Origene) were introduced into NIH3T3 and HeLa cells using Lipofectamine 3000 transfection reagent according to manufacturer’s instructions. ..

    Article Title: 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol treatment inhibits abnormal tumor growth by regulating neutrophil infiltration in a non-small cell lung carcinoma mouse model.
    Article Snippet: Excessive neutrophil infiltration into the tumor microenvironment (TME) is an important factor that contributes to tumor overgrowth and limited immunotherapy efficacy.. Neutrophils activate various receptors involved in tumor progression, while suppressing the infiltration and activity of cytotoxic T cells and creating optimal conditions for tumor growth.. Therefore, the appropriate control of neutrophil infiltration is an effective strategy for tumor treatment.

    Expressing:

    Article Title: CREG1 promotes lysosomal biogenesis and function.
    Article Snippet: .. For the expression of the CREG1-GFP fusion protein and tag-less CREG1, the HsCREG1 cDNA was subcloned in frame from pcDNA3.1 to pEGFP-N3 and pLPCX-IRESGPF, respectively. pCMV6-CREG1, pCMV6, and the shRNA plasmids targeting mouse Creg1 as well as the scrambled control were purchased from Origene Technologies (RC201654, PS100001, and TF510293, respectively). ..

    Control:

    Article Title: CREG1 promotes lysosomal biogenesis and function.
    Article Snippet: .. For the expression of the CREG1-GFP fusion protein and tag-less CREG1, the HsCREG1 cDNA was subcloned in frame from pcDNA3.1 to pEGFP-N3 and pLPCX-IRESGPF, respectively. pCMV6-CREG1, pCMV6, and the shRNA plasmids targeting mouse Creg1 as well as the scrambled control were purchased from Origene Technologies (RC201654, PS100001, and TF510293, respectively). ..

    Article Title: Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPARγ binodal complementary modules
    Article Snippet: Cells were incubated with the 5 μM CellRox Deep Red Reagent (Invitrogen) at 37 °C for 30 min and Hoescht for 20 min, washed and imaged using a confocal microscope at excitation/emission maxima for the reagent 644/665 nm. .. shRNA plasmids, including a non-target control (#TR30013) and several shRNA targeting ASK1 (FI37885, #FI37886, #FI37887, #FI37888; Origene) were transfected using Lipofectamine 2000 (Invitrogen). ..

    Infection:

    Article Title: A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation.
    Article Snippet: .. Lentivirus production and cell infection Recombination lentiviruses for QKI5 overexpression were produced using pCDH-based constructs and lentiviruses for knockdown of indicated proteins were produced using shRNA plasmids purchased from Origene (Origene Technologies, Rockville, MD, USA). .. Matching lentivirus packaging was purchased from System Biosciences (System Biosciences, Palo Alto, CA, USA) and used according to the manufacturer’s instructions.

    Transfection:

    Article Title: Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPARγ binodal complementary modules
    Article Snippet: Cells were incubated with the 5 μM CellRox Deep Red Reagent (Invitrogen) at 37 °C for 30 min and Hoescht for 20 min, washed and imaged using a confocal microscope at excitation/emission maxima for the reagent 644/665 nm. .. shRNA plasmids, including a non-target control (#TR30013) and several shRNA targeting ASK1 (FI37885, #FI37886, #FI37887, #FI37888; Origene) were transfected using Lipofectamine 2000 (Invitrogen). ..

    Article Title: Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage
    Article Snippet: RFP-Rab5 140 and 180 mutants were generated with primers (5′-GCAGCAGCTGTTGACTTCCAGG-3′ and 5′-ATTTGCTAAGTCAGCTTTGTTTCCTGAC-3′) and (5′-GCAGCGCTGCCAAAGAATGAAC-3′ and 5′-AGCTATTGCCATAAATATTTCATTTACATTCATTG-3′), respectively, using a KOD-plus mutagenesis kit (Toyobo, Osaka, Japan). .. NIH3T3 and HeLa cells were incubated in DMEM containing 10% FBS, 100 units of penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified atmosphere with 5% CO 2 . shRNA plasmids targeting human or mouse PARP1 or scrambled shRNA (Origene) were introduced into NIH3T3 and HeLa cells using Lipofectamine 3000 transfection reagent according to manufacturer’s instructions. ..

    Stable Transfection:

    Article Title: Immunomodulatory Properties of Polysaccharides from the Coral Pseudopterogorgia americana in Macrophages
    Article Snippet: .. Stable PKC-α and PKC-δ knockdown RAW264.7 and mock cells were obtained from stable transfection with shRNA plasmids that target PKC-α (catalogue number: TG501653), PKC-δ (catalogue number: TR513052) and a scrambled sequence (catalogue number: TR30012), respectively, which were obtained from OriGene Technologies, Inc. (Rockville, MD, USA). .. The RAW-Blue cell line is a RAW264.7 cell-based NF-κB reporter cell line that was obtained from InvivoGen (San Diego, CA, USA).

    Sequencing:

    Article Title: Immunomodulatory Properties of Polysaccharides from the Coral Pseudopterogorgia americana in Macrophages
    Article Snippet: .. Stable PKC-α and PKC-δ knockdown RAW264.7 and mock cells were obtained from stable transfection with shRNA plasmids that target PKC-α (catalogue number: TG501653), PKC-δ (catalogue number: TR513052) and a scrambled sequence (catalogue number: TR30012), respectively, which were obtained from OriGene Technologies, Inc. (Rockville, MD, USA). .. The RAW-Blue cell line is a RAW264.7 cell-based NF-κB reporter cell line that was obtained from InvivoGen (San Diego, CA, USA).

    Incubation:

    Article Title: Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage
    Article Snippet: RFP-Rab5 140 and 180 mutants were generated with primers (5′-GCAGCAGCTGTTGACTTCCAGG-3′ and 5′-ATTTGCTAAGTCAGCTTTGTTTCCTGAC-3′) and (5′-GCAGCGCTGCCAAAGAATGAAC-3′ and 5′-AGCTATTGCCATAAATATTTCATTTACATTCATTG-3′), respectively, using a KOD-plus mutagenesis kit (Toyobo, Osaka, Japan). .. NIH3T3 and HeLa cells were incubated in DMEM containing 10% FBS, 100 units of penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified atmosphere with 5% CO 2 . shRNA plasmids targeting human or mouse PARP1 or scrambled shRNA (Origene) were introduced into NIH3T3 and HeLa cells using Lipofectamine 3000 transfection reagent according to manufacturer’s instructions. ..

    other:

    Article Title: CREG1 promotes lysosomal biogenesis and function
    Article Snippet: The cDNAs for human wild-type CREG1 (Hs CREG1 ) was provided by Dr. Grace Gill of Tufts University (Boston, MA) and subcloned into pcDNA3.1 (Thermo Fisher Scientific, V80020) with an N-terminal MYC-tag.



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