Journal: MedComm

Article Title: Therapeutic Effects of Noninvasive Electrical Stimulation in Combination Transplantation of Human Adipose‐Derived Stem Cells‐Derived Dopaminergic Neuron on the Monkey Model of Parkinson's Disease

doi: 10.1002/mco2.70595

Figure Lengend Snippet: NES and NES‐DN attenuate the immune response by lowering SERPINA3. (A) RNA seq analysis revealed changes in 48 genes coregulated by NES and NES‐DN treatments, as shown in the Venn diagram. (B) Western blot results of SERPINA3 in putamen. C, Quantitative statistical results of changes in SERPINA3 protein levels. WT ( n = 3), MPTP ( n = 3), NES ( n = 2), and NES‐DN ( n = 3), **** p < 0.001. (D) STRING analysis shows that SERPINA3 can regulate IL‐6 and IL‐1β. (E and F) QPCR results display IL‐6 (E) and IL‐1β (F) RNA quantification statistical chart. (G) RNA seq analysis reveals a heatmap of immune related gene changes in MPTP monkeys after treatment. (H) Western blot results of immune related proteins in putamen. (I–K) Quantitative statistical results of changes in immune related protein levels of IL6 (I), TNF‐α (J), p‐p65/p65 (K). WT ( n = 3), MPTP ( n = 3), NES ( n = 2), and NES‐DN ( n = 3), * p < 0.05, ** p < 0.01, *** p < 0.005.

Article Snippet: Primary antibodies were used: TH (Invitrogen; PA5‐85167), Vinculin (Sigma; MAB3574), GAPDH (Proteintech; 60004‐1‐Ig), MNF1 (abcam; ab126575), MNF2 (CST; 9482S), OPA1 (CST; 80471S), DRP1 (abcam; ab56788), Fis1 (CST; 32525S), NeuN (abcam; ab177487), PSD95 (CST; 2507S), synapsin‐1 (CST; 5297S), synaptophysin (CST; 25056S), Iba1 (abcam; ab178846), GFAP (Invitrogen; 13‐0300), SERPINA3 (Proteintech; 12192‐1‐AP), NF‐kB p65 (CST; 8242S), IL6 (abcam; ab233551), and TNF‐a (CST; 3707S).

Techniques: RNA Sequencing, Western Blot

Journal: bioRxiv

Article Title: JointMap identified SERPINA3 + chondrocytes as a therapeutic target for osteoarthritis

doi: 10.1101/2025.09.09.675251

Figure Lengend Snippet: Arthritic transcriptional change in SERPINA3 + chondrocytes. (A) Schematic diagram showing degenerated articular cartilage surface in OA patients. (B-C) Anatomical sampling and immunostaining of DAPI (blue) and SERPINA3 (red) for chondrocyte subtype 14/15 in human OA samples (B) and mouse MMT-OA samples (C). On the left, schematic diagram showing the mouse MMT-OA model, and bar plots demonstrating the significantly elevated protein level of SERPINA3. Abbreviations including femur (F), tibia (T), and arbitrary unit (a. u.). (D-F) Enrichment analysis for DEGs in OA through the DO (D-E) and GO (F) in chondrocyte subtype 14. Abbreviations including adjusted (Adj.), fold-change (FC), cancer (Can.), and development (Dev.).

Article Snippet: Antibodies utilized in this paper included SERPINA3 (Signalway antibody, 32122), CD31 (Proteintech 11265-1-AP), CD68 (Abcam ab303565), COL II (Abcam ab34712), CTSK (Abcam ab187647), and NCL (Active Motif 39541).

Techniques: Sampling, Immunostaining

Journal: bioRxiv

Article Title: JointMap identified SERPINA3 + chondrocytes as a therapeutic target for osteoarthritis

doi: 10.1101/2025.09.09.675251

Figure Lengend Snippet: Arthritic transcriptional change in SERPINA3 + chondrocytes. (A-B) Heatmap (A) presenting the DEGs in OA and net plots (B) showing the KEGG enrichment analysis in chondrocyte subtype 14. Abbreviation including signaling pathway (SP). (C-G) Enrichment for DEGs in OA (C) through KEGG (D), DO (E-F), and GO (G) enrichment analysis in chondrocyte subtype 15. Abbreviations including degeneration (Deg.) and response (Res.).

Article Snippet: Antibodies utilized in this paper included SERPINA3 (Signalway antibody, 32122), CD31 (Proteintech 11265-1-AP), CD68 (Abcam ab303565), COL II (Abcam ab34712), CTSK (Abcam ab187647), and NCL (Active Motif 39541).

Techniques:

Journal: bioRxiv

Article Title: JointMap identified SERPINA3 + chondrocytes as a therapeutic target for osteoarthritis

doi: 10.1101/2025.09.09.675251

Figure Lengend Snippet: SERPINA3 promotes chondrogenesis in human OA chondrocytes. (A) Schematic diagram showing the in vitro experiments on human OA chondrocytes. (B) Immunostaining of SOX9 (red) and COLII (green) in hPSC-derived chondrocytes. (C) Heatmap showing the average expression normalized by the expression of GAPDH of SERPINA3 + chondrocytes and other chondrocytes under OA condition. (D) Bar plots showing the results of quantitative polymerase chain reaction (qPCR). (E) Left photographs and right bar plots showing the results of Western blot normalized to GAPDH expression. Abbreviation including kilodalton (kDa).

Article Snippet: Antibodies utilized in this paper included SERPINA3 (Signalway antibody, 32122), CD31 (Proteintech 11265-1-AP), CD68 (Abcam ab303565), COL II (Abcam ab34712), CTSK (Abcam ab187647), and NCL (Active Motif 39541).

Techniques: In Vitro, Immunostaining, Derivative Assay, Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: bioRxiv

Article Title: JointMap identified SERPINA3 + chondrocytes as a therapeutic target for osteoarthritis

doi: 10.1101/2025.09.09.675251

Figure Lengend Snippet: SERPINA3 promotes chondrogenesis in human OA chondrocytes. Uncropped membranes showing the Western blot of SERPINA3 (A), GAPDH (B), SOX9 (C), COLII (D), and ACAN (E).

Article Snippet: Antibodies utilized in this paper included SERPINA3 (Signalway antibody, 32122), CD31 (Proteintech 11265-1-AP), CD68 (Abcam ab303565), COL II (Abcam ab34712), CTSK (Abcam ab187647), and NCL (Active Motif 39541).

Techniques: Western Blot

Journal: bioRxiv

Article Title: JointMap identified SERPINA3 + chondrocytes as a therapeutic target for osteoarthritis

doi: 10.1101/2025.09.09.675251

Figure Lengend Snippet: Microenvironment and extracellular matrix. (A) Circular dot plots presenting the crosstalk among cell types of the chondral microenvironment under normal, OA, and RA conditions speculated by CellChat . The arrow from source cells (expressing ligands) pointing at the target cells (expressing receptors). The edge width representing the normalized numbers of ligand-receptor pairs. (B) Annotation heatmaps on the top showing detailed information, including p value, probability (Prob.) of cell–cell communication, standard deviation (SD), and average (Ave.) expression. Heatmaps presenting the scores (normalized average expression of chondrocytes) of genes (ligands in the left heatmap or receptors in the right heatmap). (C) Anatomical sampling and immunostaining of DAPI (blue), SERPINA3 (green) for chondrocyte subtype 14/15, and NCL (red) in human OA samples. (D) Bar plots demonstrating the elevated protein level of NCL in mouse MMT-OA model. (E) Heatmaps presenting the scores (normalized average expression of chondrocytes) of genes related to biomechanical stain (GSE165874; left) and ECM from AmiGO (right) .

Article Snippet: Antibodies utilized in this paper included SERPINA3 (Signalway antibody, 32122), CD31 (Proteintech 11265-1-AP), CD68 (Abcam ab303565), COL II (Abcam ab34712), CTSK (Abcam ab187647), and NCL (Active Motif 39541).

Techniques: Expressing, Standard Deviation, Sampling, Immunostaining, Staining

Journal: bioRxiv

Article Title: JointMap identified SERPINA3 + chondrocytes as a therapeutic target for osteoarthritis

doi: 10.1101/2025.09.09.675251

Figure Lengend Snippet: Microenvironment and extracellular matrix. (A) Circular dot plots presenting the crosstalk among cell types of chondral microenvironment under regenerative, degenerated, and Sox9 deficient conditions speculated by CellChat . (B) Heatmaps presenting the scores (normalized average expression of ImmCs) of genes (ligands in left heatmap or receptors in right heatmap). (C) Anatomical sampling and immunostaining of DAPI (blue), SERPINA3 (green) for chondrocyte subtype 14/15, and NCL (red) in mouse MMT-OA model. (D) Heatmaps presenting the scores (normalized average expression of ImmCs) of genes related to biomechanical stain (GSE165874; left) and ECM from AmiGO (right) .

Article Snippet: Antibodies utilized in this paper included SERPINA3 (Signalway antibody, 32122), CD31 (Proteintech 11265-1-AP), CD68 (Abcam ab303565), COL II (Abcam ab34712), CTSK (Abcam ab187647), and NCL (Active Motif 39541).

Techniques: Expressing, Sampling, Immunostaining, Staining

Journal: Scientific Reports

Article Title: Identification of liver proteins as biomarker for postmortem diagnosis of heat stroke through proteomics

doi: 10.1038/s41598-025-00963-x

Figure Lengend Snippet: Three candidate protein biomarkers (HSPA4, GGCX, and CYP2B6) were significantly different between HS human cases and control cases. ( a ) Representative pictures of IHC staining from three candidate biomarkers (HSPA4, GGCX, and CYP2B6) in death from HS group and control group. (200 ×, Bar: 100 μm; 400 ×, Bar: 50 μm). ( b ) Statistical analysis of mean density values for the IHC staining between HS group and control group (***: P ≤ 0.001; ****: P ≤ 0.0005).

Article Snippet: The PVDF membranes were incubated in 5% skim milk for 2 h and then incubated in diluted primary antibody RASA4 (1:1500, PAB43047 ), SERPINA3 C (1:1000, 12,192–1-AP), HSPA4 (1:1000, 21,206–1-AP), GGCX (1:1000, PAB33008 ), CYP2B6 (1:1000, PAB32079 ), ACTB (1:2000, BM-3873, Boster), and GAPDH (1:120,00, 10,494–1-AP, Proteintech) at 4 °C overnight.

Techniques: Control, Immunohistochemistry

Journal: Scientific Reports

Article Title: Identification of liver proteins as biomarker for postmortem diagnosis of heat stroke through proteomics

doi: 10.1038/s41598-025-00963-x

Figure Lengend Snippet: ROC curve analysis results of the diagnostic efficiency for HSPA4, GGCX, CYP2B6, and combination of the two proteins. AUC: Area Under the Curve.

Article Snippet: The PVDF membranes were incubated in 5% skim milk for 2 h and then incubated in diluted primary antibody RASA4 (1:1500, PAB43047 ), SERPINA3 C (1:1000, 12,192–1-AP), HSPA4 (1:1000, 21,206–1-AP), GGCX (1:1000, PAB33008 ), CYP2B6 (1:1000, PAB32079 ), ACTB (1:2000, BM-3873, Boster), and GAPDH (1:120,00, 10,494–1-AP, Proteintech) at 4 °C overnight.

Techniques: Diagnostic Assay