Journal: Neuron

Article Title: Alzheimer’s risk factors age, APOE genotype, and sex drive distinct molecular pathways

doi: 10.1016/j.neuron.2020.02.034

Figure Lengend Snippet: (A, D and G) Module preservation in the ROSMAP human prefrontal cortex (A, n = 313), Mayo clinic human temporal cortex (D, n = 159), and Mayo clinic human cerebellum (G, n = 158) datasets. Preservation Z summary between 2 and 10 indicates moderate preservation. Z summary >10 indicates strong preservation. (B and C) Blue (B) and pink (C) MEs in human control, MCI and AD samples in the ROSMAP prefrontal cortex dataset (n = 85 Ctrl, n = 78 MCI, and n = 150 AD). (E and F) Blue (E) and pink (F) MEs in human AD and controls samples in the Mayo Clinic temporal cortex dataset (n = 77 Ctrl and n = 82 AD). (H and I) Blue (H) and pink (I) MEs in human AD and controls samples in the Mayo Clinic cerebellum dataset (n = 76 Ctrl and n = 82 AD). (J-M) The gene expression levels (log2 transformed RPKM) of SERPINA3 and ATF4, two lightcyan module genes in the Mayo Clinic human temporal cortex dataset. (J, L) The expression levels of SERPINA3 (J) and ATF4 (L) between APOE4− (n = 108, including 69 Ctrl and 39 AD) and APOE4+ (n = 51, including 8 Ctrl and 43 AD) samples: left panel: expression values not adjusted by AD status; right panel: expression values adjusted by AD status. (K, M) The expression levels of SERPINA3 (K) and ATF4 (M) between AD (n = 82, including 39 APOE4− and 43 APOE4+) and Ctrl (n = 77, including 69 APOE4− and 8 APOE4+) samples: left panel: expression values not adjusted by APOE4 status; right panel: expression values adjusted by APOE4 status. In all box plots, the upper and lower lines in the boxplots represent the maximum and minimum values after Tukey’s test. The center line represents the median. P values were calculated by Mann-Whitney U tests.

Article Snippet: The Serpina3 level in mouse serum was measured using a commercial ELISA kit (Cat# CSBE13727m, Cusabio) according to the instructions provided by the manufacturer.

Techniques: Preserving, Control, Gene Expression, Transformation Assay, Expressing, MANN-WHITNEY

Journal: Neuron

Article Title: Alzheimer’s risk factors age, APOE genotype, and sex drive distinct molecular pathways

doi: 10.1016/j.neuron.2020.02.034

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The Serpina3 level in mouse serum was measured using a commercial ELISA kit (Cat# CSBE13727m, Cusabio) according to the instructions provided by the manufacturer.

Techniques: Sample Prep, Sequencing, Software, Enzyme-linked Immunosorbent Assay, RNAscope, Multiplex Assay