Journal: bioRxiv

Article Title: Dual effects of nicotinamide on aging-related arrhythmia: protective at low dose, proarrhythmic at high doses

doi: 10.1101/2025.09.22.677785

Figure Lengend Snippet: (A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with 0 mM NAM (control) or 100 mM NAM-supplemented buffer. (B) Representative electrocardiogram (ECG) shows that acute perfusion with 100 mM NAM rapidly induced VF, contractile dysfunction, and eventually led to cardiac arrest (n=4 males, 2 females). (C) Representiative blots and (D) quantification of acetylated SERCA2a levels of left venticular tissue from control and 100 mM NAM-perfused ex vivo mouse hearts (n=3). Data are expressed as mean ± standard error of the mean. Two tailed unpaired Student t-test was used.

Article Snippet: The membrane was then blocked with Intercept Blocking Buffer (927-60001, LI-COR) for 1 h at RT, and incubated with primary antibodies against SERCA2a (1:1000 dilution, 4388, cell signaling), and Pan Acetylation (1:2000 dilution, 66289-1-Ig, Proteintech) overnight at 4°C.

Techniques: Ex Vivo, Control, Two Tailed Test