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seldi proteinchip gold array  (Bio-Rad)


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    Structured Review

    Bio-Rad seldi proteinchip gold array
    (A) <t>SELDI</t> time-of-flight mass spectrum of unmodified rhTIMP-1 shows a molecular mass of 24,775 Da. (B) Mass spectrum of PEG 5K -TIMP-1 shows a molecular mass range of 46,529 Da –62,101 Da, indicating conjugation of 4–7 mPEG-5K chains; the most abundant species possesses 5 mPEG-5K chains. The smaller peaks in the range of 23,398–28,524 are consistent with doubly charged ions of the PEGylated species bearing 4–6 mPEG chains. (C) Mass spectrum of PEG 20K -TIMP-1 shows a molecular mass range of 45,139 Da –65,476 Da, indicating attachment of 1–2 mPEG-20K chains, with the mono-PEGylated species predominant. Doubly charged ions for the mono- and di-PEGylated species are also present, as is a peak for unmodified rhTIMP-1.
    Seldi Proteinchip Gold Array, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seldi+proteinchip+gold+array/ProteinChip+SPA+Matrix/pmc03502186-54-30-36
    Average 90 stars, based on 12 article reviews
    seldi proteinchip gold array - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "PEGylation Extends Circulation Half-Life While Preserving In Vitro and In Vivo Activity of Tissue Inhibitor of Metalloproteinases-1 (TIMP-1)"

    Article Title: PEGylation Extends Circulation Half-Life While Preserving In Vitro and In Vivo Activity of Tissue Inhibitor of Metalloproteinases-1 (TIMP-1)

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050028

    (A) SELDI time-of-flight mass spectrum of unmodified rhTIMP-1 shows a molecular mass of 24,775 Da. (B) Mass spectrum of PEG 5K -TIMP-1 shows a molecular mass range of 46,529 Da –62,101 Da, indicating conjugation of 4–7 mPEG-5K chains; the most abundant species possesses 5 mPEG-5K chains. The smaller peaks in the range of 23,398–28,524 are consistent with doubly charged ions of the PEGylated species bearing 4–6 mPEG chains. (C) Mass spectrum of PEG 20K -TIMP-1 shows a molecular mass range of 45,139 Da –65,476 Da, indicating attachment of 1–2 mPEG-20K chains, with the mono-PEGylated species predominant. Doubly charged ions for the mono- and di-PEGylated species are also present, as is a peak for unmodified rhTIMP-1.
    Figure Legend Snippet: (A) SELDI time-of-flight mass spectrum of unmodified rhTIMP-1 shows a molecular mass of 24,775 Da. (B) Mass spectrum of PEG 5K -TIMP-1 shows a molecular mass range of 46,529 Da –62,101 Da, indicating conjugation of 4–7 mPEG-5K chains; the most abundant species possesses 5 mPEG-5K chains. The smaller peaks in the range of 23,398–28,524 are consistent with doubly charged ions of the PEGylated species bearing 4–6 mPEG chains. (C) Mass spectrum of PEG 20K -TIMP-1 shows a molecular mass range of 45,139 Da –65,476 Da, indicating attachment of 1–2 mPEG-20K chains, with the mono-PEGylated species predominant. Doubly charged ions for the mono- and di-PEGylated species are also present, as is a peak for unmodified rhTIMP-1.

    Techniques Used: Conjugation Assay

    Related Articles

    Conjugation Assay:

    Article Title: PEGylation Extends Circulation Half-Life While Preserving In Vitro and In Vivo Activity of Tissue Inhibitor of Metalloproteinases-1 (TIMP-1)
    Article Snippet: Distribution of PEGylated species in preparations of PEG 20K -TIMP-1 and PEG 5K -TIMP-1 was analyzed on a Bio-Rad ProteinChip SELDI time-of-flight system.Distribution of PEGylated species in preparations of PEG 20K -TIMP-1 and PEG 5K -TIMP-1 was analyzed on a Bio-Rad ProteinChip SELDI time-of-flight system.. Protein samples at 1 mg/ml were mixed in 1∶4 protein-to-matrix volume with the matrix (saturated sinapinic acid in 50% acetonitrile and 0.1% trifluoroacetic acid), and spotted (1 µL) onto a SELDI ProteinChip Gold Array (A-H Format, Bio-Rad).. The mass-charge ratios ( m/ z) of TIMP-PEG species were determined using external calibration standards, All-In-One Protein Standards II (Bio-Rad) consisting of recombinant hirudin (7 kDa), cytochrome c (12 kDa), myoglobin (17 kDa), carbonic anhydrase (29 kDa), enolase (47 kDa), albumin (66 kDa) and IgG proteins (147 kDa).The mass-charge ratios ( m/ z) of TIMP-PEG species were determined using external calibration standards, All-In-One Protein Standards II (Bio-Rad) consisting of recombinant hirudin (7 kDa), cytochrome c (12 kDa), myoglobin (17 kDa), carbonic anhydrase (29 kDa), enolase (47 kDa), albumin (66 kDa) and IgG proteins (147 kDa).



    Similar Products

    seldi proteinchip gold array  (Bio-Rad)
    90
    Bio-Rad seldi proteinchip gold array
    (A) <t>SELDI</t> time-of-flight mass spectrum of unmodified rhTIMP-1 shows a molecular mass of 24,775 Da. (B) Mass spectrum of PEG 5K -TIMP-1 shows a molecular mass range of 46,529 Da –62,101 Da, indicating conjugation of 4–7 mPEG-5K chains; the most abundant species possesses 5 mPEG-5K chains. The smaller peaks in the range of 23,398–28,524 are consistent with doubly charged ions of the PEGylated species bearing 4–6 mPEG chains. (C) Mass spectrum of PEG 20K -TIMP-1 shows a molecular mass range of 45,139 Da –65,476 Da, indicating attachment of 1–2 mPEG-20K chains, with the mono-PEGylated species predominant. Doubly charged ions for the mono- and di-PEGylated species are also present, as is a peak for unmodified rhTIMP-1.
    Seldi Proteinchip Gold Array, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seldi+proteinchip+gold+array/ProteinChip+SPA+Matrix/pmc03502186-54-30-36
    Average 90 stars, based on 1 article reviews
    seldi proteinchip gold array - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) SELDI time-of-flight mass spectrum of unmodified rhTIMP-1 shows a molecular mass of 24,775 Da. (B) Mass spectrum of PEG 5K -TIMP-1 shows a molecular mass range of 46,529 Da –62,101 Da, indicating conjugation of 4–7 mPEG-5K chains; the most abundant species possesses 5 mPEG-5K chains. The smaller peaks in the range of 23,398–28,524 are consistent with doubly charged ions of the PEGylated species bearing 4–6 mPEG chains. (C) Mass spectrum of PEG 20K -TIMP-1 shows a molecular mass range of 45,139 Da –65,476 Da, indicating attachment of 1–2 mPEG-20K chains, with the mono-PEGylated species predominant. Doubly charged ions for the mono- and di-PEGylated species are also present, as is a peak for unmodified rhTIMP-1.

    Journal: PLoS ONE

    Article Title: PEGylation Extends Circulation Half-Life While Preserving In Vitro and In Vivo Activity of Tissue Inhibitor of Metalloproteinases-1 (TIMP-1)

    doi: 10.1371/journal.pone.0050028

    Figure Lengend Snippet: (A) SELDI time-of-flight mass spectrum of unmodified rhTIMP-1 shows a molecular mass of 24,775 Da. (B) Mass spectrum of PEG 5K -TIMP-1 shows a molecular mass range of 46,529 Da –62,101 Da, indicating conjugation of 4–7 mPEG-5K chains; the most abundant species possesses 5 mPEG-5K chains. The smaller peaks in the range of 23,398–28,524 are consistent with doubly charged ions of the PEGylated species bearing 4–6 mPEG chains. (C) Mass spectrum of PEG 20K -TIMP-1 shows a molecular mass range of 45,139 Da –65,476 Da, indicating attachment of 1–2 mPEG-20K chains, with the mono-PEGylated species predominant. Doubly charged ions for the mono- and di-PEGylated species are also present, as is a peak for unmodified rhTIMP-1.

    Article Snippet: Protein samples at 1 mg/ml were mixed in 1∶4 protein-to-matrix volume with the matrix (saturated sinapinic acid in 50% acetonitrile and 0.1% trifluoroacetic acid), and spotted (1 μL) onto a SELDI ProteinChip Gold Array (A-H Format, Bio-Rad).

    Techniques: Conjugation Assay