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mcherry sec61b  (Addgene inc)


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    Structured Review

    Addgene inc mcherry sec61b
    Mcherry Sec61b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sec61b+mcherry/CMV-R-GECO1+(Plasmid+%2332444)/pm41844235-220-9-10
    Average 93 stars, based on 84 article reviews
    mcherry sec61b - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: Visualization and tracking of tubule-derived, fluorescent-labeled NS1 as a marker of bluetongue virus in living cells
    Article Snippet: All mutant constructs were synthesized and cloned into the pUC57 vector, ensuring placement under control of the T7 RNA polymerase promoter and restriction enzyme recognition sites (Beijing Tsingke Biotech Co., Ltd.).

    Plasmid Preparation:

    Article Title: Ratiometric measurement of MAM Ca 2+ dynamics using a modified CalfluxVTN
    Article Snippet: .. Sec61b-mCherry was a gift from Gia Voeltz (Addgene plasmid # 49155) . pCMV R-CEPIA1er was a gift from Masamitsu Iino (Addgene plasmid # 58216) . ..



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    Subcellular localization and aggresome formation of NS1. ( a and b ) BSR cells were transfected with the lifeact-mCherry, <t>sec61b-mCherry,</t> and LAMP1-mCherry expression plasmids for 12 h, infected with BTV1-NS1-552eGFP (MOI = 0.1), fixed, and then infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1) and probed with antibodies against β-tubulin and γ-tubulin to label cell microtubules and the MTOC (red). Hoechst 33342 staining (blue). Bar, 20 µm. ( c ) BSR cells were infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1), fixed, and probed with antibodies against vimentin (red). The nuclei were stained with Hoechst 33342 (blue). Bar, 20 µm.
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    Subcellular localization and aggresome formation of NS1. ( a and b ) BSR cells were transfected with the lifeact-mCherry, <t>sec61b-mCherry,</t> and LAMP1-mCherry expression plasmids for 12 h, infected with BTV1-NS1-552eGFP (MOI = 0.1), fixed, and then infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1) and probed with antibodies against β-tubulin and γ-tubulin to label cell microtubules and the MTOC (red). Hoechst 33342 staining (blue). Bar, 20 µm. ( c ) BSR cells were infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1), fixed, and probed with antibodies against vimentin (red). The nuclei were stained with Hoechst 33342 (blue). Bar, 20 µm.
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    Image Search Results


    Subcellular localization and aggresome formation of NS1. ( a and b ) BSR cells were transfected with the lifeact-mCherry, sec61b-mCherry, and LAMP1-mCherry expression plasmids for 12 h, infected with BTV1-NS1-552eGFP (MOI = 0.1), fixed, and then infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1) and probed with antibodies against β-tubulin and γ-tubulin to label cell microtubules and the MTOC (red). Hoechst 33342 staining (blue). Bar, 20 µm. ( c ) BSR cells were infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1), fixed, and probed with antibodies against vimentin (red). The nuclei were stained with Hoechst 33342 (blue). Bar, 20 µm.

    Journal: Journal of Virology

    Article Title: Visualization and tracking of tubule-derived, fluorescent-labeled NS1 as a marker of bluetongue virus in living cells

    doi: 10.1128/jvi.00896-25

    Figure Lengend Snippet: Subcellular localization and aggresome formation of NS1. ( a and b ) BSR cells were transfected with the lifeact-mCherry, sec61b-mCherry, and LAMP1-mCherry expression plasmids for 12 h, infected with BTV1-NS1-552eGFP (MOI = 0.1), fixed, and then infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1) and probed with antibodies against β-tubulin and γ-tubulin to label cell microtubules and the MTOC (red). Hoechst 33342 staining (blue). Bar, 20 µm. ( c ) BSR cells were infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1), fixed, and probed with antibodies against vimentin (red). The nuclei were stained with Hoechst 33342 (blue). Bar, 20 µm.

    Article Snippet: Lifeact-mCherry (catalog no. 193300), sec61b-mCherry (catalog no. 90994), and LAMP1-mCherry (catalog no. 45147) were purchased from Addgene (Watertown, MA, USA).

    Techniques: Transfection, Expressing, Infection, Staining