Review



sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page protein loading buffer  (Servicebio Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Servicebio Inc sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page protein loading buffer
    Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Sds Page Protein Loading Buffer, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+gel+loading+buffer/buffer+loading/pmc13143750-102-9-21
    Average 86 stars, based on 1 article reviews
    sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page protein loading buffer - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Protein Concentration:

    Article Title: Target-Stabilized Base Editors Enable Robust High-Fidelity RNA Editing
    Article Snippet: .. Protein concentration was measured using the Pierce BCA Protein Assay Kit (23225, Thermo Scientific), after which the samples were mixed with 5×SDS-PAGE Protein Loading Buffer (G2075, Servicebio) and denatured by heating. .. Protein separation was performed via SDS-PAGE electrophoresis according to standard procedures, followed by transfer to 0.45-μm polyvinylidene fluoride (PVDF) membranes (Merck Millipore).

    Bicinchoninic Acid Protein Assay:

    Article Title: Target-Stabilized Base Editors Enable Robust High-Fidelity RNA Editing
    Article Snippet: .. Protein concentration was measured using the Pierce BCA Protein Assay Kit (23225, Thermo Scientific), after which the samples were mixed with 5×SDS-PAGE Protein Loading Buffer (G2075, Servicebio) and denatured by heating. .. Protein separation was performed via SDS-PAGE electrophoresis according to standard procedures, followed by transfer to 0.45-μm polyvinylidene fluoride (PVDF) membranes (Merck Millipore).

    Nucleic Acid Electrophoresis:

    Article Title: IL-17-producing γδ T cells in the tumor microenvironment promote radioresistance in mice
    Article Snippet: The lysates were centrifuged at 12,000×g for 30 minutes at 4°C, and the supernatant 588 was collected for protein quantification using the BCA protein assay kit (Servicebio, G2026). .. 589 Protein samples were denatured in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) loading 590 buffer (Servicebio, G2075) by boiling at 100°C for 10 minutes. ..

    Article Title: Pak4-mediated crosstalk between necroptotic macrophages and tendon stem/progenitor cells contributes to traumatic heterotopic ossification formation
    Article Snippet: Protein concentrations were determined using a bicinchoninic acid (BCA) protein detection kit (Servicebio, Wuhan, China). .. The protein samples (30 μg each) were mixed with sodium dodecyl sulfate‐polyacrylamide gel electrophoresis loading buffer (SDS‐PAGE) (Servicebio, Wuhan, China), followed by heat treatment at 100 °C for 5 min. ..

    Article Title: IL-17–producing γδ T cells in the tumor microenvironment promote radioresistance in mice
    Article Snippet: The lysates were centrifuged at 12,000 g for 30 minutes at 4°C, and the supernatant was collected for protein quantification using the BCA protein assay kit (Servicebio, G2026). .. Protein samples were denatured in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer (Servicebio, G2075) by boiling at 100°C for 10 minutes. ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Identification of novel protein biomarkers for knee osteoarthritis by integrating human plasma proteome: evidence from Mendelian randomization and preliminary in vitro investigation
    Article Snippet: TA BCA protein assay kit (Cat. no. G2026, Servicebio, China) was used to measure protein concentration in the supernatant. .. The protein solution was mixed with a 5 × sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) protein loading buffer (Cat. no. G2013, Servicebio, China) at a ratio of 4:1 and then heated in a 95 °C water bath for 10 min to denature the proteins, which were then separated by SDS-PAGE using a fast gel preparation kit (Cat. no. G2003, Servicebio, China) and transferred onto polyvinylidene fluoride membranes. .. The membranes were blocked with 5% non-fat milk for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit anti-NF-κB p65 (phospho S536) (1:2,000; Cat. no. ab239882, Abcam, UK), mouse anti-PRTN3 (1:1,000; Cat. no. sc-74534, Santa Cruz Biotechnology, USA), mouse anti-MPO (1:1,000; Cat. no. sc-390109, Santa Cruz Biotechnology, USA), and rabbit anti-GAPDH (1:2,000; Cat. no. GB11002, Servicebio, China).

    Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP10A Rabbit pAb Thermo Fisher Cat# PA5-120400; RRID: AB_2913972 DBI Polyclonal antibody(Rabbit) proteintech Cat# 14490-1-AP; RRID: AB_2211037 Beta Actin Polyclonal antibody proteintech Cat# 20536-1-AP; RRID: AB_10700003 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) proteintech Cat# SA00001-2; RRID: AB_2722564 Biological samples Colorectal cancer and adjacent normal tissues The Eighth Affiliated Hospital of Southern Medical University (The First People’s Hospital of Shunde Foshan) N/A Chemicals, peptides, and recombinant proteins L15 medium BIOSPECIES Cat# Bios-0009 RPMI-1640 medium Servicebio Cat# G4531-500ML Foetal Bovine Serum TOCYTO Cat# UT81304 0.25% Trypsin-EDTA Solution Servicebio Cat# G4001-100ML Penicillin-Streptomycin Solution (100×) Servicebio Cat# G4003-100ML RNAlater TM Stabilization Solution Thermo Fisher Cat# AM7021-500ml RNAiso Plus Takara Cat# 9109 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R222-01 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q311-02 ESP3I (BSMBI) Thermo Fisher Cat# ER0451 T4 DNA Ligase Vazyme Cat# C301-01 T4 Polynucleotide Kinase Servicebio Cat# G3458-500U Trelief TM 5α Chemically Competent Cell Tsingke Biotech Cat# TSC-C01 EndoFree Mini Plasmid Kit II TIANGEN BIOTECH (BEIJING) Cat# DP118 TIANgel Midi Purification Kit TIANGEN BIOTECH (BEIJING) Cat# DP209 RIPA Lysis Buffer Beyotime Cat# P0013B 5× SDS-PAGE Protein Loading Buffer (Odorless, Reducing) Servicebio Cat# G2075-10ML One-Step PAGE Gel Fast Preparation Kit (8%) Vazyme Cat# E302-01 One-Step PAGE Gel Fast Preparation Kit (12%) Vazyme Cat# E304-01 PVDF,0.2 μm Merck millipore Cat# ISEQ00010 Omni-ECL TM Femto Light Chemiluminescence Kit Shanghai Yazyme Cat# SQ201 Triton X-100 Beyotime Cat# P0096-100ml 4% Paraformaldehyde (General Purpose Tissue Fixative) Biosharp Cat# BL539A Critical commercial assays BeyoClick TM EdU Cell Proliferation Kit with Alexa Fluor 488 Beyotime Cat# C0071S Cell Counting Kit-8 Beyotime Cat# C0038 (Continued on next page) e1 iScience 28, 114150, December 19, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw targeted lipidomics data This paper https://dev.metabolomicsworkbench.org:22222/ data/DRCCMetadata.php?Mode=Study&StudyID= ST003864&Access=QerE9948 (Metabolomics Workbench Project ID: PR002420) UCSC Xena: TCGA TARGET GTEX UCSC Xena 42 https://xenabrowser.net/ GWAS: GCST012879 Huyghe et al. 43 https://www.ebi.ac.uk/gwas/home FinnGen: R10_C3_COLORECTAL_EXALLC FinnGen database 44 https://r10.risteys.finngen.fi/endpoints/ C3_COLORECTAL_EXALLC GWAS: GCST90277238-GCST90277416 Ottensmann et al. 45 https://www.ebi.ac.uk/gwas/home GEO: GSE10950 Jiang et al. 46 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE10950 GEO: GSE21510 Tsukamoto et al. 47 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE21510 GEO: GSE22598 Okazaki et al. 48 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE22598 GEO: GSE24514 Alhopuro et al. 49 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE24514 GEO: GSE25071 A ̊ gesen et al. 50 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE25071 GEO: GSE32323 Khamas et al. 51 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE32323 GEO: GSE35279 Kagawa et al. 52 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE35279 GEO: GSE37364 Valcz G Molná r et al. 53 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE37364 GEO: GSE41328 Lin et al. 54 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE41328 GEO: GSE44076 Dı́ez-Villanueva et al. 55 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE44076 GEO: GSE50746 Yamagishi et al. 56 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE50746 GEO: GSE110223 Vlachavas et al. 57 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE87211 Hu et al. 58 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE39582 Marisa et al. 59 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE39582 GEO: GSE17536 Smith et al. 60 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17536 GEO: GSE17537 Smith et al. 61 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17537 GEO: GSE132465 Lee et al. 62 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE132465 Experimental models: Cell lines SW620 Servicebio Cat# STCC10812P DLD-1 Shanghai Jinyuan Biotechnology Cat# JY056 Oligonucleotides See Tables S4 and S5 N/A Software and algorithms R v4.2.3 v4.4.1 R Core Team (2024) https://www.R-project.org/ (Continued on next page) iScience 28, 114150, December 19, 2025 e2

    SDS Page:

    Article Title: Identification of novel protein biomarkers for knee osteoarthritis by integrating human plasma proteome: evidence from Mendelian randomization and preliminary in vitro investigation
    Article Snippet: TA BCA protein assay kit (Cat. no. G2026, Servicebio, China) was used to measure protein concentration in the supernatant. .. The protein solution was mixed with a 5 × sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) protein loading buffer (Cat. no. G2013, Servicebio, China) at a ratio of 4:1 and then heated in a 95 °C water bath for 10 min to denature the proteins, which were then separated by SDS-PAGE using a fast gel preparation kit (Cat. no. G2003, Servicebio, China) and transferred onto polyvinylidene fluoride membranes. .. The membranes were blocked with 5% non-fat milk for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit anti-NF-κB p65 (phospho S536) (1:2,000; Cat. no. ab239882, Abcam, UK), mouse anti-PRTN3 (1:1,000; Cat. no. sc-74534, Santa Cruz Biotechnology, USA), mouse anti-MPO (1:1,000; Cat. no. sc-390109, Santa Cruz Biotechnology, USA), and rabbit anti-GAPDH (1:2,000; Cat. no. GB11002, Servicebio, China).

    Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP10A Rabbit pAb Thermo Fisher Cat# PA5-120400; RRID: AB_2913972 DBI Polyclonal antibody(Rabbit) proteintech Cat# 14490-1-AP; RRID: AB_2211037 Beta Actin Polyclonal antibody proteintech Cat# 20536-1-AP; RRID: AB_10700003 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) proteintech Cat# SA00001-2; RRID: AB_2722564 Biological samples Colorectal cancer and adjacent normal tissues The Eighth Affiliated Hospital of Southern Medical University (The First People’s Hospital of Shunde Foshan) N/A Chemicals, peptides, and recombinant proteins L15 medium BIOSPECIES Cat# Bios-0009 RPMI-1640 medium Servicebio Cat# G4531-500ML Foetal Bovine Serum TOCYTO Cat# UT81304 0.25% Trypsin-EDTA Solution Servicebio Cat# G4001-100ML Penicillin-Streptomycin Solution (100×) Servicebio Cat# G4003-100ML RNAlater TM Stabilization Solution Thermo Fisher Cat# AM7021-500ml RNAiso Plus Takara Cat# 9109 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R222-01 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q311-02 ESP3I (BSMBI) Thermo Fisher Cat# ER0451 T4 DNA Ligase Vazyme Cat# C301-01 T4 Polynucleotide Kinase Servicebio Cat# G3458-500U Trelief TM 5α Chemically Competent Cell Tsingke Biotech Cat# TSC-C01 EndoFree Mini Plasmid Kit II TIANGEN BIOTECH (BEIJING) Cat# DP118 TIANgel Midi Purification Kit TIANGEN BIOTECH (BEIJING) Cat# DP209 RIPA Lysis Buffer Beyotime Cat# P0013B 5× SDS-PAGE Protein Loading Buffer (Odorless, Reducing) Servicebio Cat# G2075-10ML One-Step PAGE Gel Fast Preparation Kit (8%) Vazyme Cat# E302-01 One-Step PAGE Gel Fast Preparation Kit (12%) Vazyme Cat# E304-01 PVDF,0.2 μm Merck millipore Cat# ISEQ00010 Omni-ECL TM Femto Light Chemiluminescence Kit Shanghai Yazyme Cat# SQ201 Triton X-100 Beyotime Cat# P0096-100ml 4% Paraformaldehyde (General Purpose Tissue Fixative) Biosharp Cat# BL539A Critical commercial assays BeyoClick TM EdU Cell Proliferation Kit with Alexa Fluor 488 Beyotime Cat# C0071S Cell Counting Kit-8 Beyotime Cat# C0038 (Continued on next page) e1 iScience 28, 114150, December 19, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw targeted lipidomics data This paper https://dev.metabolomicsworkbench.org:22222/ data/DRCCMetadata.php?Mode=Study&StudyID= ST003864&Access=QerE9948 (Metabolomics Workbench Project ID: PR002420) UCSC Xena: TCGA TARGET GTEX UCSC Xena 42 https://xenabrowser.net/ GWAS: GCST012879 Huyghe et al. 43 https://www.ebi.ac.uk/gwas/home FinnGen: R10_C3_COLORECTAL_EXALLC FinnGen database 44 https://r10.risteys.finngen.fi/endpoints/ C3_COLORECTAL_EXALLC GWAS: GCST90277238-GCST90277416 Ottensmann et al. 45 https://www.ebi.ac.uk/gwas/home GEO: GSE10950 Jiang et al. 46 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE10950 GEO: GSE21510 Tsukamoto et al. 47 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE21510 GEO: GSE22598 Okazaki et al. 48 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE22598 GEO: GSE24514 Alhopuro et al. 49 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE24514 GEO: GSE25071 A ̊ gesen et al. 50 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE25071 GEO: GSE32323 Khamas et al. 51 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE32323 GEO: GSE35279 Kagawa et al. 52 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE35279 GEO: GSE37364 Valcz G Molná r et al. 53 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE37364 GEO: GSE41328 Lin et al. 54 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE41328 GEO: GSE44076 Dı́ez-Villanueva et al. 55 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE44076 GEO: GSE50746 Yamagishi et al. 56 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE50746 GEO: GSE110223 Vlachavas et al. 57 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE87211 Hu et al. 58 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE39582 Marisa et al. 59 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE39582 GEO: GSE17536 Smith et al. 60 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17536 GEO: GSE17537 Smith et al. 61 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17537 GEO: GSE132465 Lee et al. 62 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE132465 Experimental models: Cell lines SW620 Servicebio Cat# STCC10812P DLD-1 Shanghai Jinyuan Biotechnology Cat# JY056 Oligonucleotides See Tables S4 and S5 N/A Software and algorithms R v4.2.3 v4.4.1 R Core Team (2024) https://www.R-project.org/ (Continued on next page) iScience 28, 114150, December 19, 2025 e2

    Article Title: High expression of the underexplored SLC4A11 protein-coding transcript is specific to the corneal endothelium.
    Article Snippet: The cells were detached from a 6-well plate using 0.25% w/v Trypsin-EDTA (Thermo Fisher Scientific; Waltham, Massachusetts, USA). .. Lysates were AR TIC LE IN PR ES S prepared using 2x SDS-PAGE Loading Buffer (Non-Reduced) (G2032-1ML, Servicebio) supplemented with beta-mercaptoethanol to a concentration of 5%. ..

    Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions
    Article Snippet: RIPA Lysis Buffer , Beyotime , Cat# P0013B. .. 5× SDS-PAGE Protein Loading Buffer (Odorless, Reducing) , Servicebio , Cat# G2075-10ML. .. One-Step PAGE Gel Fast Preparation Kit (8%) , Vazyme , Cat# E302-01.

    Recombinant:

    Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP10A Rabbit pAb Thermo Fisher Cat# PA5-120400; RRID: AB_2913972 DBI Polyclonal antibody(Rabbit) proteintech Cat# 14490-1-AP; RRID: AB_2211037 Beta Actin Polyclonal antibody proteintech Cat# 20536-1-AP; RRID: AB_10700003 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) proteintech Cat# SA00001-2; RRID: AB_2722564 Biological samples Colorectal cancer and adjacent normal tissues The Eighth Affiliated Hospital of Southern Medical University (The First People’s Hospital of Shunde Foshan) N/A Chemicals, peptides, and recombinant proteins L15 medium BIOSPECIES Cat# Bios-0009 RPMI-1640 medium Servicebio Cat# G4531-500ML Foetal Bovine Serum TOCYTO Cat# UT81304 0.25% Trypsin-EDTA Solution Servicebio Cat# G4001-100ML Penicillin-Streptomycin Solution (100×) Servicebio Cat# G4003-100ML RNAlater TM Stabilization Solution Thermo Fisher Cat# AM7021-500ml RNAiso Plus Takara Cat# 9109 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R222-01 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q311-02 ESP3I (BSMBI) Thermo Fisher Cat# ER0451 T4 DNA Ligase Vazyme Cat# C301-01 T4 Polynucleotide Kinase Servicebio Cat# G3458-500U Trelief TM 5α Chemically Competent Cell Tsingke Biotech Cat# TSC-C01 EndoFree Mini Plasmid Kit II TIANGEN BIOTECH (BEIJING) Cat# DP118 TIANgel Midi Purification Kit TIANGEN BIOTECH (BEIJING) Cat# DP209 RIPA Lysis Buffer Beyotime Cat# P0013B 5× SDS-PAGE Protein Loading Buffer (Odorless, Reducing) Servicebio Cat# G2075-10ML One-Step PAGE Gel Fast Preparation Kit (8%) Vazyme Cat# E302-01 One-Step PAGE Gel Fast Preparation Kit (12%) Vazyme Cat# E304-01 PVDF,0.2 μm Merck millipore Cat# ISEQ00010 Omni-ECL TM Femto Light Chemiluminescence Kit Shanghai Yazyme Cat# SQ201 Triton X-100 Beyotime Cat# P0096-100ml 4% Paraformaldehyde (General Purpose Tissue Fixative) Biosharp Cat# BL539A Critical commercial assays BeyoClick TM EdU Cell Proliferation Kit with Alexa Fluor 488 Beyotime Cat# C0071S Cell Counting Kit-8 Beyotime Cat# C0038 (Continued on next page) e1 iScience 28, 114150, December 19, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw targeted lipidomics data This paper https://dev.metabolomicsworkbench.org:22222/ data/DRCCMetadata.php?Mode=Study&StudyID= ST003864&Access=QerE9948 (Metabolomics Workbench Project ID: PR002420) UCSC Xena: TCGA TARGET GTEX UCSC Xena 42 https://xenabrowser.net/ GWAS: GCST012879 Huyghe et al. 43 https://www.ebi.ac.uk/gwas/home FinnGen: R10_C3_COLORECTAL_EXALLC FinnGen database 44 https://r10.risteys.finngen.fi/endpoints/ C3_COLORECTAL_EXALLC GWAS: GCST90277238-GCST90277416 Ottensmann et al. 45 https://www.ebi.ac.uk/gwas/home GEO: GSE10950 Jiang et al. 46 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE10950 GEO: GSE21510 Tsukamoto et al. 47 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE21510 GEO: GSE22598 Okazaki et al. 48 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE22598 GEO: GSE24514 Alhopuro et al. 49 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE24514 GEO: GSE25071 A ̊ gesen et al. 50 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE25071 GEO: GSE32323 Khamas et al. 51 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE32323 GEO: GSE35279 Kagawa et al. 52 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE35279 GEO: GSE37364 Valcz G Molná r et al. 53 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE37364 GEO: GSE41328 Lin et al. 54 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE41328 GEO: GSE44076 Dı́ez-Villanueva et al. 55 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE44076 GEO: GSE50746 Yamagishi et al. 56 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE50746 GEO: GSE110223 Vlachavas et al. 57 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE87211 Hu et al. 58 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE39582 Marisa et al. 59 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE39582 GEO: GSE17536 Smith et al. 60 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17536 GEO: GSE17537 Smith et al. 61 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17537 GEO: GSE132465 Lee et al. 62 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE132465 Experimental models: Cell lines SW620 Servicebio Cat# STCC10812P DLD-1 Shanghai Jinyuan Biotechnology Cat# JY056 Oligonucleotides See Tables S4 and S5 N/A Software and algorithms R v4.2.3 v4.4.1 R Core Team (2024) https://www.R-project.org/ (Continued on next page) iScience 28, 114150, December 19, 2025 e2

    Real-time Polymerase Chain Reaction:

    Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP10A Rabbit pAb Thermo Fisher Cat# PA5-120400; RRID: AB_2913972 DBI Polyclonal antibody(Rabbit) proteintech Cat# 14490-1-AP; RRID: AB_2211037 Beta Actin Polyclonal antibody proteintech Cat# 20536-1-AP; RRID: AB_10700003 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) proteintech Cat# SA00001-2; RRID: AB_2722564 Biological samples Colorectal cancer and adjacent normal tissues The Eighth Affiliated Hospital of Southern Medical University (The First People’s Hospital of Shunde Foshan) N/A Chemicals, peptides, and recombinant proteins L15 medium BIOSPECIES Cat# Bios-0009 RPMI-1640 medium Servicebio Cat# G4531-500ML Foetal Bovine Serum TOCYTO Cat# UT81304 0.25% Trypsin-EDTA Solution Servicebio Cat# G4001-100ML Penicillin-Streptomycin Solution (100×) Servicebio Cat# G4003-100ML RNAlater TM Stabilization Solution Thermo Fisher Cat# AM7021-500ml RNAiso Plus Takara Cat# 9109 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R222-01 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q311-02 ESP3I (BSMBI) Thermo Fisher Cat# ER0451 T4 DNA Ligase Vazyme Cat# C301-01 T4 Polynucleotide Kinase Servicebio Cat# G3458-500U Trelief TM 5α Chemically Competent Cell Tsingke Biotech Cat# TSC-C01 EndoFree Mini Plasmid Kit II TIANGEN BIOTECH (BEIJING) Cat# DP118 TIANgel Midi Purification Kit TIANGEN BIOTECH (BEIJING) Cat# DP209 RIPA Lysis Buffer Beyotime Cat# P0013B 5× SDS-PAGE Protein Loading Buffer (Odorless, Reducing) Servicebio Cat# G2075-10ML One-Step PAGE Gel Fast Preparation Kit (8%) Vazyme Cat# E302-01 One-Step PAGE Gel Fast Preparation Kit (12%) Vazyme Cat# E304-01 PVDF,0.2 μm Merck millipore Cat# ISEQ00010 Omni-ECL TM Femto Light Chemiluminescence Kit Shanghai Yazyme Cat# SQ201 Triton X-100 Beyotime Cat# P0096-100ml 4% Paraformaldehyde (General Purpose Tissue Fixative) Biosharp Cat# BL539A Critical commercial assays BeyoClick TM EdU Cell Proliferation Kit with Alexa Fluor 488 Beyotime Cat# C0071S Cell Counting Kit-8 Beyotime Cat# C0038 (Continued on next page) e1 iScience 28, 114150, December 19, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw targeted lipidomics data This paper https://dev.metabolomicsworkbench.org:22222/ data/DRCCMetadata.php?Mode=Study&StudyID= ST003864&Access=QerE9948 (Metabolomics Workbench Project ID: PR002420) UCSC Xena: TCGA TARGET GTEX UCSC Xena 42 https://xenabrowser.net/ GWAS: GCST012879 Huyghe et al. 43 https://www.ebi.ac.uk/gwas/home FinnGen: R10_C3_COLORECTAL_EXALLC FinnGen database 44 https://r10.risteys.finngen.fi/endpoints/ C3_COLORECTAL_EXALLC GWAS: GCST90277238-GCST90277416 Ottensmann et al. 45 https://www.ebi.ac.uk/gwas/home GEO: GSE10950 Jiang et al. 46 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE10950 GEO: GSE21510 Tsukamoto et al. 47 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE21510 GEO: GSE22598 Okazaki et al. 48 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE22598 GEO: GSE24514 Alhopuro et al. 49 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE24514 GEO: GSE25071 A ̊ gesen et al. 50 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE25071 GEO: GSE32323 Khamas et al. 51 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE32323 GEO: GSE35279 Kagawa et al. 52 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE35279 GEO: GSE37364 Valcz G Molná r et al. 53 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE37364 GEO: GSE41328 Lin et al. 54 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE41328 GEO: GSE44076 Dı́ez-Villanueva et al. 55 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE44076 GEO: GSE50746 Yamagishi et al. 56 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE50746 GEO: GSE110223 Vlachavas et al. 57 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE87211 Hu et al. 58 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE39582 Marisa et al. 59 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE39582 GEO: GSE17536 Smith et al. 60 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17536 GEO: GSE17537 Smith et al. 61 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17537 GEO: GSE132465 Lee et al. 62 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE132465 Experimental models: Cell lines SW620 Servicebio Cat# STCC10812P DLD-1 Shanghai Jinyuan Biotechnology Cat# JY056 Oligonucleotides See Tables S4 and S5 N/A Software and algorithms R v4.2.3 v4.4.1 R Core Team (2024) https://www.R-project.org/ (Continued on next page) iScience 28, 114150, December 19, 2025 e2

    Plasmid Preparation:

    Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP10A Rabbit pAb Thermo Fisher Cat# PA5-120400; RRID: AB_2913972 DBI Polyclonal antibody(Rabbit) proteintech Cat# 14490-1-AP; RRID: AB_2211037 Beta Actin Polyclonal antibody proteintech Cat# 20536-1-AP; RRID: AB_10700003 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) proteintech Cat# SA00001-2; RRID: AB_2722564 Biological samples Colorectal cancer and adjacent normal tissues The Eighth Affiliated Hospital of Southern Medical University (The First People’s Hospital of Shunde Foshan) N/A Chemicals, peptides, and recombinant proteins L15 medium BIOSPECIES Cat# Bios-0009 RPMI-1640 medium Servicebio Cat# G4531-500ML Foetal Bovine Serum TOCYTO Cat# UT81304 0.25% Trypsin-EDTA Solution Servicebio Cat# G4001-100ML Penicillin-Streptomycin Solution (100×) Servicebio Cat# G4003-100ML RNAlater TM Stabilization Solution Thermo Fisher Cat# AM7021-500ml RNAiso Plus Takara Cat# 9109 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R222-01 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q311-02 ESP3I (BSMBI) Thermo Fisher Cat# ER0451 T4 DNA Ligase Vazyme Cat# C301-01 T4 Polynucleotide Kinase Servicebio Cat# G3458-500U Trelief TM 5α Chemically Competent Cell Tsingke Biotech Cat# TSC-C01 EndoFree Mini Plasmid Kit II TIANGEN BIOTECH (BEIJING) Cat# DP118 TIANgel Midi Purification Kit TIANGEN BIOTECH (BEIJING) Cat# DP209 RIPA Lysis Buffer Beyotime Cat# P0013B 5× SDS-PAGE Protein Loading Buffer (Odorless, Reducing) Servicebio Cat# G2075-10ML One-Step PAGE Gel Fast Preparation Kit (8%) Vazyme Cat# E302-01 One-Step PAGE Gel Fast Preparation Kit (12%) Vazyme Cat# E304-01 PVDF,0.2 μm Merck millipore Cat# ISEQ00010 Omni-ECL TM Femto Light Chemiluminescence Kit Shanghai Yazyme Cat# SQ201 Triton X-100 Beyotime Cat# P0096-100ml 4% Paraformaldehyde (General Purpose Tissue Fixative) Biosharp Cat# BL539A Critical commercial assays BeyoClick TM EdU Cell Proliferation Kit with Alexa Fluor 488 Beyotime Cat# C0071S Cell Counting Kit-8 Beyotime Cat# C0038 (Continued on next page) e1 iScience 28, 114150, December 19, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw targeted lipidomics data This paper https://dev.metabolomicsworkbench.org:22222/ data/DRCCMetadata.php?Mode=Study&StudyID= ST003864&Access=QerE9948 (Metabolomics Workbench Project ID: PR002420) UCSC Xena: TCGA TARGET GTEX UCSC Xena 42 https://xenabrowser.net/ GWAS: GCST012879 Huyghe et al. 43 https://www.ebi.ac.uk/gwas/home FinnGen: R10_C3_COLORECTAL_EXALLC FinnGen database 44 https://r10.risteys.finngen.fi/endpoints/ C3_COLORECTAL_EXALLC GWAS: GCST90277238-GCST90277416 Ottensmann et al. 45 https://www.ebi.ac.uk/gwas/home GEO: GSE10950 Jiang et al. 46 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE10950 GEO: GSE21510 Tsukamoto et al. 47 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE21510 GEO: GSE22598 Okazaki et al. 48 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE22598 GEO: GSE24514 Alhopuro et al. 49 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE24514 GEO: GSE25071 A ̊ gesen et al. 50 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE25071 GEO: GSE32323 Khamas et al. 51 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE32323 GEO: GSE35279 Kagawa et al. 52 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE35279 GEO: GSE37364 Valcz G Molná r et al. 53 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE37364 GEO: GSE41328 Lin et al. 54 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE41328 GEO: GSE44076 Dı́ez-Villanueva et al. 55 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE44076 GEO: GSE50746 Yamagishi et al. 56 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE50746 GEO: GSE110223 Vlachavas et al. 57 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE87211 Hu et al. 58 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE39582 Marisa et al. 59 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE39582 GEO: GSE17536 Smith et al. 60 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17536 GEO: GSE17537 Smith et al. 61 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17537 GEO: GSE132465 Lee et al. 62 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE132465 Experimental models: Cell lines SW620 Servicebio Cat# STCC10812P DLD-1 Shanghai Jinyuan Biotechnology Cat# JY056 Oligonucleotides See Tables S4 and S5 N/A Software and algorithms R v4.2.3 v4.4.1 R Core Team (2024) https://www.R-project.org/ (Continued on next page) iScience 28, 114150, December 19, 2025 e2

    Purification:

    Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP10A Rabbit pAb Thermo Fisher Cat# PA5-120400; RRID: AB_2913972 DBI Polyclonal antibody(Rabbit) proteintech Cat# 14490-1-AP; RRID: AB_2211037 Beta Actin Polyclonal antibody proteintech Cat# 20536-1-AP; RRID: AB_10700003 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) proteintech Cat# SA00001-2; RRID: AB_2722564 Biological samples Colorectal cancer and adjacent normal tissues The Eighth Affiliated Hospital of Southern Medical University (The First People’s Hospital of Shunde Foshan) N/A Chemicals, peptides, and recombinant proteins L15 medium BIOSPECIES Cat# Bios-0009 RPMI-1640 medium Servicebio Cat# G4531-500ML Foetal Bovine Serum TOCYTO Cat# UT81304 0.25% Trypsin-EDTA Solution Servicebio Cat# G4001-100ML Penicillin-Streptomycin Solution (100×) Servicebio Cat# G4003-100ML RNAlater TM Stabilization Solution Thermo Fisher Cat# AM7021-500ml RNAiso Plus Takara Cat# 9109 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R222-01 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q311-02 ESP3I (BSMBI) Thermo Fisher Cat# ER0451 T4 DNA Ligase Vazyme Cat# C301-01 T4 Polynucleotide Kinase Servicebio Cat# G3458-500U Trelief TM 5α Chemically Competent Cell Tsingke Biotech Cat# TSC-C01 EndoFree Mini Plasmid Kit II TIANGEN BIOTECH (BEIJING) Cat# DP118 TIANgel Midi Purification Kit TIANGEN BIOTECH (BEIJING) Cat# DP209 RIPA Lysis Buffer Beyotime Cat# P0013B 5× SDS-PAGE Protein Loading Buffer (Odorless, Reducing) Servicebio Cat# G2075-10ML One-Step PAGE Gel Fast Preparation Kit (8%) Vazyme Cat# E302-01 One-Step PAGE Gel Fast Preparation Kit (12%) Vazyme Cat# E304-01 PVDF,0.2 μm Merck millipore Cat# ISEQ00010 Omni-ECL TM Femto Light Chemiluminescence Kit Shanghai Yazyme Cat# SQ201 Triton X-100 Beyotime Cat# P0096-100ml 4% Paraformaldehyde (General Purpose Tissue Fixative) Biosharp Cat# BL539A Critical commercial assays BeyoClick TM EdU Cell Proliferation Kit with Alexa Fluor 488 Beyotime Cat# C0071S Cell Counting Kit-8 Beyotime Cat# C0038 (Continued on next page) e1 iScience 28, 114150, December 19, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw targeted lipidomics data This paper https://dev.metabolomicsworkbench.org:22222/ data/DRCCMetadata.php?Mode=Study&StudyID= ST003864&Access=QerE9948 (Metabolomics Workbench Project ID: PR002420) UCSC Xena: TCGA TARGET GTEX UCSC Xena 42 https://xenabrowser.net/ GWAS: GCST012879 Huyghe et al. 43 https://www.ebi.ac.uk/gwas/home FinnGen: R10_C3_COLORECTAL_EXALLC FinnGen database 44 https://r10.risteys.finngen.fi/endpoints/ C3_COLORECTAL_EXALLC GWAS: GCST90277238-GCST90277416 Ottensmann et al. 45 https://www.ebi.ac.uk/gwas/home GEO: GSE10950 Jiang et al. 46 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE10950 GEO: GSE21510 Tsukamoto et al. 47 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE21510 GEO: GSE22598 Okazaki et al. 48 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE22598 GEO: GSE24514 Alhopuro et al. 49 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE24514 GEO: GSE25071 A ̊ gesen et al. 50 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE25071 GEO: GSE32323 Khamas et al. 51 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE32323 GEO: GSE35279 Kagawa et al. 52 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE35279 GEO: GSE37364 Valcz G Molná r et al. 53 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE37364 GEO: GSE41328 Lin et al. 54 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE41328 GEO: GSE44076 Dı́ez-Villanueva et al. 55 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE44076 GEO: GSE50746 Yamagishi et al. 56 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE50746 GEO: GSE110223 Vlachavas et al. 57 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE87211 Hu et al. 58 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE39582 Marisa et al. 59 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE39582 GEO: GSE17536 Smith et al. 60 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17536 GEO: GSE17537 Smith et al. 61 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17537 GEO: GSE132465 Lee et al. 62 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE132465 Experimental models: Cell lines SW620 Servicebio Cat# STCC10812P DLD-1 Shanghai Jinyuan Biotechnology Cat# JY056 Oligonucleotides See Tables S4 and S5 N/A Software and algorithms R v4.2.3 v4.4.1 R Core Team (2024) https://www.R-project.org/ (Continued on next page) iScience 28, 114150, December 19, 2025 e2

    Lysis:

    Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP10A Rabbit pAb Thermo Fisher Cat# PA5-120400; RRID: AB_2913972 DBI Polyclonal antibody(Rabbit) proteintech Cat# 14490-1-AP; RRID: AB_2211037 Beta Actin Polyclonal antibody proteintech Cat# 20536-1-AP; RRID: AB_10700003 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) proteintech Cat# SA00001-2; RRID: AB_2722564 Biological samples Colorectal cancer and adjacent normal tissues The Eighth Affiliated Hospital of Southern Medical University (The First People’s Hospital of Shunde Foshan) N/A Chemicals, peptides, and recombinant proteins L15 medium BIOSPECIES Cat# Bios-0009 RPMI-1640 medium Servicebio Cat# G4531-500ML Foetal Bovine Serum TOCYTO Cat# UT81304 0.25% Trypsin-EDTA Solution Servicebio Cat# G4001-100ML Penicillin-Streptomycin Solution (100×) Servicebio Cat# G4003-100ML RNAlater TM Stabilization Solution Thermo Fisher Cat# AM7021-500ml RNAiso Plus Takara Cat# 9109 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R222-01 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q311-02 ESP3I (BSMBI) Thermo Fisher Cat# ER0451 T4 DNA Ligase Vazyme Cat# C301-01 T4 Polynucleotide Kinase Servicebio Cat# G3458-500U Trelief TM 5α Chemically Competent Cell Tsingke Biotech Cat# TSC-C01 EndoFree Mini Plasmid Kit II TIANGEN BIOTECH (BEIJING) Cat# DP118 TIANgel Midi Purification Kit TIANGEN BIOTECH (BEIJING) Cat# DP209 RIPA Lysis Buffer Beyotime Cat# P0013B 5× SDS-PAGE Protein Loading Buffer (Odorless, Reducing) Servicebio Cat# G2075-10ML One-Step PAGE Gel Fast Preparation Kit (8%) Vazyme Cat# E302-01 One-Step PAGE Gel Fast Preparation Kit (12%) Vazyme Cat# E304-01 PVDF,0.2 μm Merck millipore Cat# ISEQ00010 Omni-ECL TM Femto Light Chemiluminescence Kit Shanghai Yazyme Cat# SQ201 Triton X-100 Beyotime Cat# P0096-100ml 4% Paraformaldehyde (General Purpose Tissue Fixative) Biosharp Cat# BL539A Critical commercial assays BeyoClick TM EdU Cell Proliferation Kit with Alexa Fluor 488 Beyotime Cat# C0071S Cell Counting Kit-8 Beyotime Cat# C0038 (Continued on next page) e1 iScience 28, 114150, December 19, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw targeted lipidomics data This paper https://dev.metabolomicsworkbench.org:22222/ data/DRCCMetadata.php?Mode=Study&StudyID= ST003864&Access=QerE9948 (Metabolomics Workbench Project ID: PR002420) UCSC Xena: TCGA TARGET GTEX UCSC Xena 42 https://xenabrowser.net/ GWAS: GCST012879 Huyghe et al. 43 https://www.ebi.ac.uk/gwas/home FinnGen: R10_C3_COLORECTAL_EXALLC FinnGen database 44 https://r10.risteys.finngen.fi/endpoints/ C3_COLORECTAL_EXALLC GWAS: GCST90277238-GCST90277416 Ottensmann et al. 45 https://www.ebi.ac.uk/gwas/home GEO: GSE10950 Jiang et al. 46 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE10950 GEO: GSE21510 Tsukamoto et al. 47 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE21510 GEO: GSE22598 Okazaki et al. 48 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE22598 GEO: GSE24514 Alhopuro et al. 49 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE24514 GEO: GSE25071 A ̊ gesen et al. 50 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE25071 GEO: GSE32323 Khamas et al. 51 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE32323 GEO: GSE35279 Kagawa et al. 52 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE35279 GEO: GSE37364 Valcz G Molná r et al. 53 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE37364 GEO: GSE41328 Lin et al. 54 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE41328 GEO: GSE44076 Dı́ez-Villanueva et al. 55 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE44076 GEO: GSE50746 Yamagishi et al. 56 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE50746 GEO: GSE110223 Vlachavas et al. 57 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE87211 Hu et al. 58 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE39582 Marisa et al. 59 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE39582 GEO: GSE17536 Smith et al. 60 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17536 GEO: GSE17537 Smith et al. 61 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17537 GEO: GSE132465 Lee et al. 62 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE132465 Experimental models: Cell lines SW620 Servicebio Cat# STCC10812P DLD-1 Shanghai Jinyuan Biotechnology Cat# JY056 Oligonucleotides See Tables S4 and S5 N/A Software and algorithms R v4.2.3 v4.4.1 R Core Team (2024) https://www.R-project.org/ (Continued on next page) iScience 28, 114150, December 19, 2025 e2

    CCK-8 Assay:

    Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP10A Rabbit pAb Thermo Fisher Cat# PA5-120400; RRID: AB_2913972 DBI Polyclonal antibody(Rabbit) proteintech Cat# 14490-1-AP; RRID: AB_2211037 Beta Actin Polyclonal antibody proteintech Cat# 20536-1-AP; RRID: AB_10700003 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) proteintech Cat# SA00001-2; RRID: AB_2722564 Biological samples Colorectal cancer and adjacent normal tissues The Eighth Affiliated Hospital of Southern Medical University (The First People’s Hospital of Shunde Foshan) N/A Chemicals, peptides, and recombinant proteins L15 medium BIOSPECIES Cat# Bios-0009 RPMI-1640 medium Servicebio Cat# G4531-500ML Foetal Bovine Serum TOCYTO Cat# UT81304 0.25% Trypsin-EDTA Solution Servicebio Cat# G4001-100ML Penicillin-Streptomycin Solution (100×) Servicebio Cat# G4003-100ML RNAlater TM Stabilization Solution Thermo Fisher Cat# AM7021-500ml RNAiso Plus Takara Cat# 9109 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R222-01 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q311-02 ESP3I (BSMBI) Thermo Fisher Cat# ER0451 T4 DNA Ligase Vazyme Cat# C301-01 T4 Polynucleotide Kinase Servicebio Cat# G3458-500U Trelief TM 5α Chemically Competent Cell Tsingke Biotech Cat# TSC-C01 EndoFree Mini Plasmid Kit II TIANGEN BIOTECH (BEIJING) Cat# DP118 TIANgel Midi Purification Kit TIANGEN BIOTECH (BEIJING) Cat# DP209 RIPA Lysis Buffer Beyotime Cat# P0013B 5× SDS-PAGE Protein Loading Buffer (Odorless, Reducing) Servicebio Cat# G2075-10ML One-Step PAGE Gel Fast Preparation Kit (8%) Vazyme Cat# E302-01 One-Step PAGE Gel Fast Preparation Kit (12%) Vazyme Cat# E304-01 PVDF,0.2 μm Merck millipore Cat# ISEQ00010 Omni-ECL TM Femto Light Chemiluminescence Kit Shanghai Yazyme Cat# SQ201 Triton X-100 Beyotime Cat# P0096-100ml 4% Paraformaldehyde (General Purpose Tissue Fixative) Biosharp Cat# BL539A Critical commercial assays BeyoClick TM EdU Cell Proliferation Kit with Alexa Fluor 488 Beyotime Cat# C0071S Cell Counting Kit-8 Beyotime Cat# C0038 (Continued on next page) e1 iScience 28, 114150, December 19, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw targeted lipidomics data This paper https://dev.metabolomicsworkbench.org:22222/ data/DRCCMetadata.php?Mode=Study&StudyID= ST003864&Access=QerE9948 (Metabolomics Workbench Project ID: PR002420) UCSC Xena: TCGA TARGET GTEX UCSC Xena 42 https://xenabrowser.net/ GWAS: GCST012879 Huyghe et al. 43 https://www.ebi.ac.uk/gwas/home FinnGen: R10_C3_COLORECTAL_EXALLC FinnGen database 44 https://r10.risteys.finngen.fi/endpoints/ C3_COLORECTAL_EXALLC GWAS: GCST90277238-GCST90277416 Ottensmann et al. 45 https://www.ebi.ac.uk/gwas/home GEO: GSE10950 Jiang et al. 46 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE10950 GEO: GSE21510 Tsukamoto et al. 47 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE21510 GEO: GSE22598 Okazaki et al. 48 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE22598 GEO: GSE24514 Alhopuro et al. 49 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE24514 GEO: GSE25071 A ̊ gesen et al. 50 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE25071 GEO: GSE32323 Khamas et al. 51 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE32323 GEO: GSE35279 Kagawa et al. 52 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE35279 GEO: GSE37364 Valcz G Molná r et al. 53 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE37364 GEO: GSE41328 Lin et al. 54 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE41328 GEO: GSE44076 Dı́ez-Villanueva et al. 55 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE44076 GEO: GSE50746 Yamagishi et al. 56 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE50746 GEO: GSE110223 Vlachavas et al. 57 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE87211 Hu et al. 58 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE110223 GEO: GSE39582 Marisa et al. 59 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE39582 GEO: GSE17536 Smith et al. 60 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17536 GEO: GSE17537 Smith et al. 61 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE17537 GEO: GSE132465 Lee et al. 62 https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE132465 Experimental models: Cell lines SW620 Servicebio Cat# STCC10812P DLD-1 Shanghai Jinyuan Biotechnology Cat# JY056 Oligonucleotides See Tables S4 and S5 N/A Software and algorithms R v4.2.3 v4.4.1 R Core Team (2024) https://www.R-project.org/ (Continued on next page) iScience 28, 114150, December 19, 2025 e2

    Concentration Assay:

    Article Title: High expression of the underexplored SLC4A11 protein-coding transcript is specific to the corneal endothelium.
    Article Snippet: The cells were detached from a 6-well plate using 0.25% w/v Trypsin-EDTA (Thermo Fisher Scientific; Waltham, Massachusetts, USA). .. Lysates were AR TIC LE IN PR ES S prepared using 2x SDS-PAGE Loading Buffer (Non-Reduced) (G2032-1ML, Servicebio) supplemented with beta-mercaptoethanol to a concentration of 5%. ..



    Similar Products

    sds page gels  (Boster Bio)
    95
    Boster Bio sds page gels
    Sds Page Gels, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+gel+loading+buffer/SDS-PAGE+Protein+Loading+Buffer/pmc13657812-148-10-12
    Average 95 stars, based on 1 article reviews
    sds page gels - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    gel loading  (Thermo Fisher)
    99
    Thermo Fisher gel loading
    Gel Loading, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+gel+loading+buffer/Laemmli+SDS+sample+buffer%2C+reducing/pm42259771-333-17-8
    Average 99 stars, based on 1 article reviews
    gel loading - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page protein loading buffer  (Servicebio Inc)
    86
    Servicebio Inc sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page protein loading buffer
    Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Sds Page Protein Loading Buffer, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+gel+loading+buffer/buffer+loading/pmc13143750-102-9-21
    Average 86 stars, based on 1 article reviews
    sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page protein loading buffer - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page loading buffer  (Cowin Biosciences)
    86
    Cowin Biosciences sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page loading buffer
    Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Sds Page Loading Buffer, supplied by Cowin Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+gel+loading+buffer/buffer+loading+page+sds/pm41898478-265-1-11
    Average 86 stars, based on 1 article reviews
    sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page loading buffer - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    loading buffer  (New England Biolabs)
    97
    New England Biolabs loading buffer
    Loading Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+gel+loading+buffer/Gel+Loading+Dye%2C+Purple+(6X)%2C+no+SDS/bio_rxiv__64898__2026__03__19__709872-115-0-3
    Average 97 stars, based on 1 article reviews
    loading buffer - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier
    sds polyacrylamide gel electrophoresis page loading buffer  (Bio-Rad)
    98
    Bio-Rad sds polyacrylamide gel electrophoresis page loading buffer
    Sds Polyacrylamide Gel Electrophoresis Page Loading Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+gel+loading+buffer/SDS/pmc12825294-48-18-24
    Average 98 stars, based on 1 article reviews
    sds polyacrylamide gel electrophoresis page loading buffer - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    sds gel loading buffer  (Bio-Rad)
    98
    Bio-Rad sds gel loading buffer
    Sds Gel Loading Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+gel+loading+buffer/Tris/pm41634771-147-10-40
    Average 98 stars, based on 1 article reviews
    sds gel loading buffer - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page loading buffer  (Bio-Rad)
    97
    Bio-Rad sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page loading buffer
    (a) Schematic of TAMRA peptide IVG assay. Incubation of TAMRA-labeled peptides with OSTs, Cj LLOs, and divalent metal ions (e.g., Mn 2+ ) results in transfer of heptasaccharide glycans to form glycopeptide products. (b) Tricine <t>SDS-PAGE</t> analysis of IVG reactions with different amounts of TAMRA-labeled peptide and 0.5 μM of each OST. Graph at right depicts determination of Michaelis–Menten kinetics using IVG reaction data. Data fitting was by nonlinear regression analysis according to Michaelis–Menten model using Prism 10 for MacOS (version 10.3.0). Black arrows denote aglycosylated (g0) and singly glycosylated (g1) forms of TAMRA-labeled peptides. Tricine SDS-PAGE gels in each panel are representative of three biological replicates. Data in corresponding graphs are average of biological replicates ( n = 3) ± SD.
    Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Sds Page Loading Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+gel+loading+buffer/SDS+(Sodium+Dodecyl+Sulfate)/bio_rxiv__64898__2026__01__30__702934-210-7-43
    Average 97 stars, based on 1 article reviews
    sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page loading buffer - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) Schematic of TAMRA peptide IVG assay. Incubation of TAMRA-labeled peptides with OSTs, Cj LLOs, and divalent metal ions (e.g., Mn 2+ ) results in transfer of heptasaccharide glycans to form glycopeptide products. (b) Tricine SDS-PAGE analysis of IVG reactions with different amounts of TAMRA-labeled peptide and 0.5 μM of each OST. Graph at right depicts determination of Michaelis–Menten kinetics using IVG reaction data. Data fitting was by nonlinear regression analysis according to Michaelis–Menten model using Prism 10 for MacOS (version 10.3.0). Black arrows denote aglycosylated (g0) and singly glycosylated (g1) forms of TAMRA-labeled peptides. Tricine SDS-PAGE gels in each panel are representative of three biological replicates. Data in corresponding graphs are average of biological replicates ( n = 3) ± SD.

    Journal: bioRxiv

    Article Title: Topological reprogramming transforms an integral membrane oligosaccharyltransferase into a water-soluble glycosylation catalyst

    doi: 10.64898/2026.01.30.702934

    Figure Lengend Snippet: (a) Schematic of TAMRA peptide IVG assay. Incubation of TAMRA-labeled peptides with OSTs, Cj LLOs, and divalent metal ions (e.g., Mn 2+ ) results in transfer of heptasaccharide glycans to form glycopeptide products. (b) Tricine SDS-PAGE analysis of IVG reactions with different amounts of TAMRA-labeled peptide and 0.5 μM of each OST. Graph at right depicts determination of Michaelis–Menten kinetics using IVG reaction data. Data fitting was by nonlinear regression analysis according to Michaelis–Menten model using Prism 10 for MacOS (version 10.3.0). Black arrows denote aglycosylated (g0) and singly glycosylated (g1) forms of TAMRA-labeled peptides. Tricine SDS-PAGE gels in each panel are representative of three biological replicates. Data in corresponding graphs are average of biological replicates ( n = 3) ± SD.

    Article Snippet: Protein samples were denatured by addition of sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) loading buffer containing 10% β-mercaptoethanol, followed by heating at 90 °C for 10 min. Electrophoretic separation was performed using either precast 4–20% Tris-Glycine gels (Invitrogen) or AnyKD Mini-PROTEAN TGX gels (Bio-Rad).

    Techniques: Incubation, Labeling, Glycoproteomics, SDS Page