Journal: Frontiers in Neurology

Article Title: A phase 1 proof-of-concept study evaluating safety, tolerability, and biological marker responses with combination therapy of CTLA4-Ig and interleukin-2 in amyotrophic lateral sclerosis

doi: 10.3389/fneur.2024.1415106

Figure Lengend Snippet: Treg numbers and suppressive function increased with CTLA4-Ig/IL-2 treatment throughout the 48-week treatment period. Changes in Treg suppressive function (A) , the percentage of CD4 + CD25 + FOXP3 + cells (B) , the percentage of CD4 + CD25 + cells (C) , the percentage of CD4 + CD25 − cells (D) , and the percentage of CD8 + cells (E) throughout the study are shown. Changes in CD25 protein expression (F) and FOXP3 protein expression (G) in the CD4 + CD25 + FOXP3 + population by mean fluorescent intensity (MFI) throughout the study are shown. Data were analyzed by a one-way ANOVA with post-hoc Tukey test and p values less than 0.05 were considered significant. Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: Levels of soluble CD25 (sCD25) were also assayed by ELISA (R&D Systems, Cat# DR2A00) using sera from participants ( n = 4) and healthy controls ( n = 23) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: The Journal of Clinical Investigation

Article Title: The SWI/SNF chromatin-remodeling subunit DPF2 facilitates NRF2-dependent antiinflammatory and antioxidant gene expression

doi: 10.1172/JCI158419

Figure Lengend Snippet: ( A ) Representative images of Vav1-Cre –derived 28-day-old mice. ( B ) Total BWs of 28-day-old mice. ( C ) Kaplan-Meier survival curves; the median survival of Dpf2 Δ/Δ mice was 28 days. ( D ) Representative images of organs from 28-day-old mice. ( E ) Total number of cells in BM, spleen, and thymus. ( F ) CBC of PB in approximately 28-day-old mice ( n = 18). Hb, hemoglobin; PLT, platelets. ( G ) Representative H&E staining of BM, spleen, and thymus from 28-day-old mice. Scale bars: 50 μm. ( H and I ) Representative H&E staining of liver ( G ) and lung ( H ) from end-stage Dpf2 Δ/Δ and age-matched Dpf2 fl/fl mice. Scale bars: 200 μm. ( J ) Representative CD68/macrosialin IHC staining of lung and liver. Scale bars: 200 μm (lung IHC), 50 μm ( Dpf2 fl/fl liver), and 100 μm ( Dpf2 Δ/Δ liver). ( K ) Representative CD69 IHC staining of lung and liver infiltrates. Scale bars: 50 μm. ( L ) Plasma cytokine levels. Values correspond to the mean spot pixel density relative to background from 4 mice/genotype. ( M ) Chemistry profiling of PB from 28-day-old mice ( n = 3). ( N ) Serum ferritin and sCD25 plasma levels. All bar graph data represent the mean ± SD. *** P < 0.001, by 2-tailed, unpaired Student’s t test ( B , M , and N ) and ordinary, 1-way ANOVA ( E and F ).

Article Snippet: Plasma was used for the cytokine array analyses (R&D Systems, ARY028); the ferritin ELISA kit (ALPCO, 41-FERMS-E01); the sCD25 ELISA kit (G-Biosciences, IT5809); and chemistry profiling (HESKA; catalog 6330, COMP/EWRAP).

Techniques: Derivative Assay, Staining, Immunohistochemistry, Clinical Proteomics

Journal: The Journal of Clinical Investigation

Article Title: The SWI/SNF chromatin-remodeling subunit DPF2 facilitates NRF2-dependent antiinflammatory and antioxidant gene expression

doi: 10.1172/JCI158419

Figure Lengend Snippet: ( A ) Kaplan-Meier survival curves of mice treated with vehicle or CDDO-Im. Treatment was interrupted after 85 days (dashed line). A log-rank (Mantel-Cox) text was performed to determine significant differences in the survival of Dpf2 Δ/Δ mice treated with vehicle or CDDO-Im. ( B and C ) Expression of Dpf2 and Nrf2 ( B ), and NRF2 target genes ( C ) in LK cells from Dpf2 fl/fl and Dpf2 Δ/Δ mice treated with vehicle or CDDO-Im. P value for vehicle-treated versus CDDO-Im–treated Dpf2 Δ/Δ mouse data are indicated below each plot. ( D ) sCD25 in plasma from the indicated groups of mice. ( E ) PB counts in mice treated for 2 weeks with vehicle or CDDO-Im. ( F ) Flow cytometric analyses of splenic cell populations. Data represent the mean ± SEM. P values were calculated using 2-way ANOVA.

Article Snippet: Plasma was used for the cytokine array analyses (R&D Systems, ARY028); the ferritin ELISA kit (ALPCO, 41-FERMS-E01); the sCD25 ELISA kit (G-Biosciences, IT5809); and chemistry profiling (HESKA; catalog 6330, COMP/EWRAP).

Techniques: Expressing, Clinical Proteomics