Journal: The Journal of Clinical Investigation

Article Title: The SWI/SNF chromatin-remodeling subunit DPF2 facilitates NRF2-dependent antiinflammatory and antioxidant gene expression

doi: 10.1172/JCI158419

Figure Lengend Snippet: ( A ) Representative images of Vav1-Cre –derived 28-day-old mice. ( B ) Total BWs of 28-day-old mice. ( C ) Kaplan-Meier survival curves; the median survival of Dpf2 Δ/Δ mice was 28 days. ( D ) Representative images of organs from 28-day-old mice. ( E ) Total number of cells in BM, spleen, and thymus. ( F ) CBC of PB in approximately 28-day-old mice ( n = 18). Hb, hemoglobin; PLT, platelets. ( G ) Representative H&E staining of BM, spleen, and thymus from 28-day-old mice. Scale bars: 50 μm. ( H and I ) Representative H&E staining of liver ( G ) and lung ( H ) from end-stage Dpf2 Δ/Δ and age-matched Dpf2 fl/fl mice. Scale bars: 200 μm. ( J ) Representative CD68/macrosialin IHC staining of lung and liver. Scale bars: 200 μm (lung IHC), 50 μm ( Dpf2 fl/fl liver), and 100 μm ( Dpf2 Δ/Δ liver). ( K ) Representative CD69 IHC staining of lung and liver infiltrates. Scale bars: 50 μm. ( L ) Plasma cytokine levels. Values correspond to the mean spot pixel density relative to background from 4 mice/genotype. ( M ) Chemistry profiling of PB from 28-day-old mice ( n = 3). ( N ) Serum ferritin and sCD25 plasma levels. All bar graph data represent the mean ± SD. *** P < 0.001, by 2-tailed, unpaired Student’s t test ( B , M , and N ) and ordinary, 1-way ANOVA ( E and F ).

Article Snippet: Plasma was used for the cytokine array analyses (R&D Systems, ARY028); the ferritin ELISA kit (ALPCO, 41-FERMS-E01); the sCD25 ELISA kit (G-Biosciences, IT5809); and chemistry profiling (HESKA; catalog 6330, COMP/EWRAP).

Techniques: Derivative Assay, Staining, Immunohistochemistry, Clinical Proteomics

Journal: The Journal of Clinical Investigation

Article Title: The SWI/SNF chromatin-remodeling subunit DPF2 facilitates NRF2-dependent antiinflammatory and antioxidant gene expression

doi: 10.1172/JCI158419

Figure Lengend Snippet: ( A ) Kaplan-Meier survival curves of mice treated with vehicle or CDDO-Im. Treatment was interrupted after 85 days (dashed line). A log-rank (Mantel-Cox) text was performed to determine significant differences in the survival of Dpf2 Δ/Δ mice treated with vehicle or CDDO-Im. ( B and C ) Expression of Dpf2 and Nrf2 ( B ), and NRF2 target genes ( C ) in LK cells from Dpf2 fl/fl and Dpf2 Δ/Δ mice treated with vehicle or CDDO-Im. P value for vehicle-treated versus CDDO-Im–treated Dpf2 Δ/Δ mouse data are indicated below each plot. ( D ) sCD25 in plasma from the indicated groups of mice. ( E ) PB counts in mice treated for 2 weeks with vehicle or CDDO-Im. ( F ) Flow cytometric analyses of splenic cell populations. Data represent the mean ± SEM. P values were calculated using 2-way ANOVA.

Article Snippet: Plasma was used for the cytokine array analyses (R&D Systems, ARY028); the ferritin ELISA kit (ALPCO, 41-FERMS-E01); the sCD25 ELISA kit (G-Biosciences, IT5809); and chemistry profiling (HESKA; catalog 6330, COMP/EWRAP).

Techniques: Expressing, Clinical Proteomics

Journal: British Journal of Cancer

Article Title: A phase 1 trial of recombinant human IL-21 in combination with cetuximab in patients with metastatic colorectal cancer

doi: 10.1038/bjc.2011.599

Figure Lengend Snippet: Mean serum sCD25 (U ml −1 ).

Article Snippet: Cytotoxicity towards NK-sensitive K562 target cells, activation markers on NK cells and monocytes, as well as soluble IL-2 receptor-alpha (sCD25) were measured at Esoterix Clinical Trial Services, Belgium.

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Specific sequences of infectious challenge lead to secondary hemophagocytic lymphohistiocytosis-like disease in mice

doi: 10.1073/pnas.1820704116

Figure Lengend Snippet: IC:LPS induces sHLH-like phenotype in mice. (A–C) Plasma ferritin (A), triglycerides (B), ALT (C) was assessed following LPS and Poly I:C administered at the indicated time points (arrows) in the indicated order (n = 5 per group). (D–F) Complete blood counts were performed on mice challenged sequentially at t = 0 and then t = 24 h with LPS, Poly I:C, or vehicle (PBS) in the indicated order. White blood cells (D), hemoglobin (E), and platelets (F) are displayed. (G) Soluble CD25 was assessed following LPS and Poly I:C administered at the indicated time points (arrows) in the indicated order (n = 5 per group). (H) Representative cytospin images of hemophagocytosis (arrows) from bone marrow aspirates harvested from mice 4 h after challenge with IC:LPS (Right) with quantification (Left). ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. (Scale bars, 20 µm.)

Article Snippet: Plasma ferritin concentrations (Abcam) were determined using the kit according to the manufacturer’s protocols. sCD25 concentrations (MyBioSource) were determined using the kit according to the manufacturer’s protocols.

Techniques: