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sb431542  (Tocris)


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Bioz Manufacturer Symbol Tocris manufactures this product  
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    Structured Review

    Tocris sb431542
    Sb431542, supplied by Tocris, used in various techniques. Bioz Stars score: 97/100, based on 2557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sb+431542/SB+431542/pm42894639-134-72-73
    Average 97 stars, based on 2557 article reviews
    sb431542 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycle
    Article Snippet: The next day, spheres were collected and transferred to 10 cm ultra-low-attachment dishes (Corning, 3262). .. For the first 6 days, they were cultured in Essential 6 medium (Thermo Fisher Scientific, A1516401) with SB-431542 (10 μM, Tocris, 1614), dorsomorphin (2.5 μM Sigma-Aldrich, P5499), and XAV-939 (2.5 μM, Tocris, 3748), with daily medium changes. .. From Day 7, hCOs were maintained in Neurobasal A medium (Thermo Fisher Scientific, 10888) containing B27 (Thermo Fisher Scientific, 12587), along with 20 ng/ml epidermal growth factor (R&D Systems, 236-EG) and 20 ng/ml basic fibroblast growth factor (R&D Systems, 233-FB), with daily medium changes for 8 days, followed by changes every other day until Day 25.

    Knock-Out:

    Article Title: Multiomics analysis identifies VPA-induced changes in neural progenitor cells, ventricular-like regions, and cellular microenvironment in dorsal forebrain organoids.
    Article Snippet: Dorsal forebrain organoid differentiation Dorsal forebrain organoids were generated by using previously described protocol [23] with minor changes. iPSCs that reached around 80% confluency were treated with Accutase (Merck, Cat. no. A6964) to obtain single cell dissociation. .. Then, 9000 cells per well were seeded into 96 well V-bottom low adhesion plates (S-bio, Cat. no. MS-9096VZ) in cortical differentiation medium (CDM) I (Glasgow’s MEM (Gibco, Cat. no. 11710035) with 20% KnockOut Serum Replacement (KOSR, Thermofisher Scientific, Cat. no. 10828028), 1X non-essential amino acids (Sigma, Cat. no. M7145), 0.11 mg/mL Sodium Pyruvate (Thermofisher Scientific, Cat. no. 11360070), 1X Penicillin-Streptomycin (Thermofisher Scientific, Cat. no. 15140122), 0.1 mM β- Mercaptoethanol (Gibco, Cat. no. 21985023) with supplements (20 μM Y-27632 (Cayman Chemical, Cat. no. 10005583), 5 μM SB 431542 (Tocris, Cat. no. 1614), and 3 μM IWR-1 (Merck, Cat. no. 681669)). ..

    Article Title: Human brain and organoid transcriptomes reveal key receptor tyrosine kinase pathways and genetic signatures in Alzheimer's disease.
    Article Snippet: The next day, the medium was replaced with AggreWell EB Formation Medium (ST05893, StemCell Technologies). .. From day 2 to day 5, EBs were maintained with the medium composed of DMEM/F-12 containing GlutaMAX (10565018, Gibco), 20% KnockOut Serum Replacement (A3181501, Gibco), 1% MEM Non-Essential Amino Acids Solution (11140050, Gibco), 0.1 mM 2-mercaptoethanol (21985023, Gibco), 100 U/ ml penicillin and 100 μg/ml streptomycin (P4333, Merck, Darmstadt, Germany), dorsomorphin (10 μM; P5499, Merck), and SB-431542 (10 μM; 1614, TOCRIS). .. On day 6, EBs were transferred to a petri dish with a medium composed of Neurobasal-A Medium (10888-022, Gibco), B-27 Supplement minus vitamin A (12587010, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin, GlutaMAX (10565018, Gibco) Matrigel basement membrane matrix (354234, Corning), 20 ng/ml epidermal growth factor (EGF; AF-100-15, PeproTech), and 20 ng/ml fibroblast growth factor basic (bFGF; 100-18B, PeproTech).



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