sanger sequencing service (Azenta)
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Sanger Sequencing Service, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanger+sequencing+services/sanger+sequencing/pmc13271104-57-7-10
Average 86 stars, based on 1 article reviews
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Sequencing:Article Title: Two remarkable new species of Glaucocharis (Lepidoptera, Crambidae, Crambinae) from the Ogasawara Islands, Japan. Article Snippet: we describe two new species of the genus Glaucocharis from the ogasawara Islands of Japan: G. triochellaris Matsui, Yagi & Hirowatari, sp. nov. and G. plumbofascialis Matsui, Yagi & Hirowatari, sp. nov. the new species with remarkable morphological characters are easily distinguished from congeneric species. we provide photographs of adult, male and female genitalia.. Molecular phylogenetic analysis revealed a sister relationship between the two new species and the monophyly of Glaucocharis. Article Title: Redescription of immature stages of the shiitake fungus moth, Morophagoides moriutii Robinson (Lepidoptera: Tineidae) Article Snippet: .. The amplified products were purified using ExoSAP–ITTM Express (Thermo Fisher Scientific Inc., USA), and sequencing was conducted at Article Title: Somatic EPAS1 Variants in Pheochromocytoma and Paraganglioma in Patients With Sickle Cell Disease. Article Snippet: .. Purified products were sequenced in both forward and reverse directions using Article Title: A new Hibiscus-feeding species of Bucculatrix (Bucculatricidae) from the Ogasawara Islands, Japan Article Snippet: The amplicons were purified using ExoSAPIT Express (Thermo Fisher Scientific Inc., Massachusetts, USA). .. The cycle sequences reactions and bidirectional sequencing were conducted using premixed Article Title: Improved protein binder design using β-pairing targeted RFdiffusion Article Snippet: Protein binder designs were ordered as synthetic genes (eBlocks, Integrated DNA Technologies) and cloned via BsaI overhangs into the target cloning vector, LM0627 for Golden Gate assembly. .. Golden Gate reaction mixtures were transformed into a chemically competent expression strain (BL21(DE3)), and overnight outgrowth cultures were used to seed 500 mL protein expression cultures in auto-induction media (autoclaved TBII media supplemented with Kanamycin, 2 mM MgSO4, 1×5052) and to propagate expression plasmid for isolation (QIAprep Spin Miniprep Kit) and Article Title: Genome reorganisation and expansion shape 3D genome architecture and define a distinct regulatory landscape in coleoid cephalopods Article Snippet: PCR products were run on a 2% agarose (Sigma-Aldrich, A9793) gel and resulting bands were gel purified using the Monarch DNA Gel Extraction kit (NEB, T1020) following Manufacturer’s instructions. .. Purified PCR products were analyzed using Article Title: Experimental evolution in the cystic fibrosis chemical environment reveals early TCA cycle flux as a central regulator of Mycobacterium abscessus biofilm formation Article Snippet: .. PCR products with the correct fragment size were purified and sequenced using the Amplification:Article Title: Redescription of immature stages of the shiitake fungus moth, Morophagoides moriutii Robinson (Lepidoptera: Tineidae) Article Snippet: .. The amplified products were purified using ExoSAP–ITTM Express (Thermo Fisher Scientific Inc., USA), and sequencing was conducted at Purification:Article Title: Redescription of immature stages of the shiitake fungus moth, Morophagoides moriutii Robinson (Lepidoptera: Tineidae) Article Snippet: .. The amplified products were purified using ExoSAP–ITTM Express (Thermo Fisher Scientific Inc., USA), and sequencing was conducted at Article Title: Somatic EPAS1 Variants in Pheochromocytoma and Paraganglioma in Patients With Sickle Cell Disease. Article Snippet: .. Purified products were sequenced in both forward and reverse directions using Article Title: Genome reorganisation and expansion shape 3D genome architecture and define a distinct regulatory landscape in coleoid cephalopods Article Snippet: PCR products were run on a 2% agarose (Sigma-Aldrich, A9793) gel and resulting bands were gel purified using the Monarch DNA Gel Extraction kit (NEB, T1020) following Manufacturer’s instructions. .. Purified PCR products were analyzed using Article Title: Experimental evolution in the cystic fibrosis chemical environment reveals early TCA cycle flux as a central regulator of Mycobacterium abscessus biofilm formation Article Snippet: .. PCR products with the correct fragment size were purified and sequenced using the Transformation Assay:Article Title: Improved protein binder design using β-pairing targeted RFdiffusion Article Snippet: Protein binder designs were ordered as synthetic genes (eBlocks, Integrated DNA Technologies) and cloned via BsaI overhangs into the target cloning vector, LM0627 for Golden Gate assembly. .. Golden Gate reaction mixtures were transformed into a chemically competent expression strain (BL21(DE3)), and overnight outgrowth cultures were used to seed 500 mL protein expression cultures in auto-induction media (autoclaved TBII media supplemented with Kanamycin, 2 mM MgSO4, 1×5052) and to propagate expression plasmid for isolation (QIAprep Spin Miniprep Kit) and Expressing:Article Title: Improved protein binder design using β-pairing targeted RFdiffusion Article Snippet: Protein binder designs were ordered as synthetic genes (eBlocks, Integrated DNA Technologies) and cloned via BsaI overhangs into the target cloning vector, LM0627 for Golden Gate assembly. .. Golden Gate reaction mixtures were transformed into a chemically competent expression strain (BL21(DE3)), and overnight outgrowth cultures were used to seed 500 mL protein expression cultures in auto-induction media (autoclaved TBII media supplemented with Kanamycin, 2 mM MgSO4, 1×5052) and to propagate expression plasmid for isolation (QIAprep Spin Miniprep Kit) and Plasmid Preparation:Article Title: Improved protein binder design using β-pairing targeted RFdiffusion Article Snippet: Protein binder designs were ordered as synthetic genes (eBlocks, Integrated DNA Technologies) and cloned via BsaI overhangs into the target cloning vector, LM0627 for Golden Gate assembly. .. Golden Gate reaction mixtures were transformed into a chemically competent expression strain (BL21(DE3)), and overnight outgrowth cultures were used to seed 500 mL protein expression cultures in auto-induction media (autoclaved TBII media supplemented with Kanamycin, 2 mM MgSO4, 1×5052) and to propagate expression plasmid for isolation (QIAprep Spin Miniprep Kit) and Isolation:Article Title: Improved protein binder design using β-pairing targeted RFdiffusion Article Snippet: Protein binder designs were ordered as synthetic genes (eBlocks, Integrated DNA Technologies) and cloned via BsaI overhangs into the target cloning vector, LM0627 for Golden Gate assembly. .. Golden Gate reaction mixtures were transformed into a chemically competent expression strain (BL21(DE3)), and overnight outgrowth cultures were used to seed 500 mL protein expression cultures in auto-induction media (autoclaved TBII media supplemented with Kanamycin, 2 mM MgSO4, 1×5052) and to propagate expression plasmid for isolation (QIAprep Spin Miniprep Kit) and Polymerase Chain Reaction:Article Title: Genome reorganisation and expansion shape 3D genome architecture and define a distinct regulatory landscape in coleoid cephalopods Article Snippet: PCR products were run on a 2% agarose (Sigma-Aldrich, A9793) gel and resulting bands were gel purified using the Monarch DNA Gel Extraction kit (NEB, T1020) following Manufacturer’s instructions. .. Purified PCR products were analyzed using Article Title: Experimental evolution in the cystic fibrosis chemical environment reveals early TCA cycle flux as a central regulator of Mycobacterium abscessus biofilm formation Article Snippet: .. PCR products with the correct fragment size were purified and sequenced using the Mutagenesis:Article Title: Optimization of the hongqu starter preparation process for the manufacturing of red mold rice with high gamma-aminobutyric acid production by solid-state fermentation. Article Snippet: Anqi Wu and Liangyi Li contributed equally to this work.. Abstract Red mold rice (RMR) generally contains gamma-aminobutyric acid (GABA), which has several physiological functions.. Monascus purpureus M162, with a high GABA production of 15.10 mg/g was generated by atmospheric and room temperature plasma mutation. |

