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prk7 ha s6k1  (Addgene inc)


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    Structured Review

    Addgene inc prk7 ha s6k1
    Prk7 Ha S6k1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s6k1/pRK7-HA-S6K1-WT+(Plasmid+%238984)/pm41802695-76-27-28
    Average 93 stars, based on 44 article reviews
    prk7 ha s6k1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer.
    Article Snippet: .. The radiation-sensitive H23 cells were transfected with an empty vector (Addgene #10883, Watertown, MA, USA) or with a vector expressing a modified cDNA expressing a constitutively active form of S6K1 (pRK7-HA-S6K1-F5A-E389-R3A) (Addgene #8991, Watertown, MA, USA) [31,32]. ..

    Article Title: S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer
    Article Snippet: .. The radiation-sensitive H23 cells were transfected with an empty vector (Addgene #10883, Watertown, MA, USA) or with a vector expressing a modified cDNA expressing a constitutively active form of S6K1 (pRK7-HA-S6K1-F5A-E389-R3A) (Addgene #8991, Watertown, MA, USA) [ , ]. ..

    Expressing:

    Article Title: S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer.
    Article Snippet: .. The radiation-sensitive H23 cells were transfected with an empty vector (Addgene #10883, Watertown, MA, USA) or with a vector expressing a modified cDNA expressing a constitutively active form of S6K1 (pRK7-HA-S6K1-F5A-E389-R3A) (Addgene #8991, Watertown, MA, USA) [31,32]. ..

    Article Title: S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer
    Article Snippet: .. The radiation-sensitive H23 cells were transfected with an empty vector (Addgene #10883, Watertown, MA, USA) or with a vector expressing a modified cDNA expressing a constitutively active form of S6K1 (pRK7-HA-S6K1-F5A-E389-R3A) (Addgene #8991, Watertown, MA, USA) [ , ]. ..

    Modification:

    Article Title: S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer.
    Article Snippet: .. The radiation-sensitive H23 cells were transfected with an empty vector (Addgene #10883, Watertown, MA, USA) or with a vector expressing a modified cDNA expressing a constitutively active form of S6K1 (pRK7-HA-S6K1-F5A-E389-R3A) (Addgene #8991, Watertown, MA, USA) [31,32]. ..

    Article Title: S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer
    Article Snippet: .. The radiation-sensitive H23 cells were transfected with an empty vector (Addgene #10883, Watertown, MA, USA) or with a vector expressing a modified cDNA expressing a constitutively active form of S6K1 (pRK7-HA-S6K1-F5A-E389-R3A) (Addgene #8991, Watertown, MA, USA) [ , ]. ..

    Clone Assay:

    Article Title: Mammalian Target of Rapamycin Cell Signaling Pathway Contributes to the Protective Effects of Ischemic Postconditioning Against Stroke
    Article Snippet: .. The S6K1 gene was cloned from the plasmids of S6K1(pcDNA3) (S6K1: 26610; Addgene, Cambridge, MA) and inserted into the lentiviral backbone plasmid pHR’tripCMV-IRES-eGFP, which contains a cytomegalovirus (CMV) promoter and an IRES sequence between its multiple cloning sites and eGFP. ..

    Plasmid Preparation:

    Article Title: Mammalian Target of Rapamycin Cell Signaling Pathway Contributes to the Protective Effects of Ischemic Postconditioning Against Stroke
    Article Snippet: .. The S6K1 gene was cloned from the plasmids of S6K1(pcDNA3) (S6K1: 26610; Addgene, Cambridge, MA) and inserted into the lentiviral backbone plasmid pHR’tripCMV-IRES-eGFP, which contains a cytomegalovirus (CMV) promoter and an IRES sequence between its multiple cloning sites and eGFP. ..

    Sequencing:

    Article Title: Mammalian Target of Rapamycin Cell Signaling Pathway Contributes to the Protective Effects of Ischemic Postconditioning Against Stroke
    Article Snippet: .. The S6K1 gene was cloned from the plasmids of S6K1(pcDNA3) (S6K1: 26610; Addgene, Cambridge, MA) and inserted into the lentiviral backbone plasmid pHR’tripCMV-IRES-eGFP, which contains a cytomegalovirus (CMV) promoter and an IRES sequence between its multiple cloning sites and eGFP. ..

    Cloning:

    Article Title: Mammalian Target of Rapamycin Cell Signaling Pathway Contributes to the Protective Effects of Ischemic Postconditioning Against Stroke
    Article Snippet: .. The S6K1 gene was cloned from the plasmids of S6K1(pcDNA3) (S6K1: 26610; Addgene, Cambridge, MA) and inserted into the lentiviral backbone plasmid pHR’tripCMV-IRES-eGFP, which contains a cytomegalovirus (CMV) promoter and an IRES sequence between its multiple cloning sites and eGFP. ..



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    (A-G) mTOR signaling in the liver was determined by Western blot analysis of the following phosphorylated and unphosphorylated proteins; S6 (B), 4e-BP1 (C), <t>S6K1</t> (D), eiF2α (E), AKT (F-G), HSP90 (loading control). (H-J) mTORC1 signaling in the muscle was determined by Western blot analysis of phosphorylated S6 (I), phosphorylated 4e-BP1 (K). HSP90 was used as a loading control. (B-G, I-J) Quantification was determined by normalizing phosphorylated protein to normal protein. (A-J) n=5-6 mice/group, quantification was determined using ImageJ by normalizing the phosphorylated protein to the unphosphorylated form. Three-way ANOVA between sex, diet, and gonadectomy with post hoc Šidák’s adjusted test for pairwise comparisons, *p<0.05. p values for the overall effect of sex, diet, and gonadectomy and the interactions represent the significant p values from the three-way ANOVA. Data are represented as mean ±SEM. Abbreviations: C, CTL (21% control protein), L, LP (7% low protein), Cast (castration), Fem (female), Ovx (ovariectomy), Gonad (gonadectomy). Created in BioRender. Knopf, B. (2026) https://BioRender.com/i0gripn
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    Image Search Results


    (A-G) mTOR signaling in the liver was determined by Western blot analysis of the following phosphorylated and unphosphorylated proteins; S6 (B), 4e-BP1 (C), S6K1 (D), eiF2α (E), AKT (F-G), HSP90 (loading control). (H-J) mTORC1 signaling in the muscle was determined by Western blot analysis of phosphorylated S6 (I), phosphorylated 4e-BP1 (K). HSP90 was used as a loading control. (B-G, I-J) Quantification was determined by normalizing phosphorylated protein to normal protein. (A-J) n=5-6 mice/group, quantification was determined using ImageJ by normalizing the phosphorylated protein to the unphosphorylated form. Three-way ANOVA between sex, diet, and gonadectomy with post hoc Šidák’s adjusted test for pairwise comparisons, *p<0.05. p values for the overall effect of sex, diet, and gonadectomy and the interactions represent the significant p values from the three-way ANOVA. Data are represented as mean ±SEM. Abbreviations: C, CTL (21% control protein), L, LP (7% low protein), Cast (castration), Fem (female), Ovx (ovariectomy), Gonad (gonadectomy). Created in BioRender. Knopf, B. (2026) https://BioRender.com/i0gripn

    Journal: bioRxiv

    Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice

    doi: 10.64898/2026.03.31.715667

    Figure Lengend Snippet: (A-G) mTOR signaling in the liver was determined by Western blot analysis of the following phosphorylated and unphosphorylated proteins; S6 (B), 4e-BP1 (C), S6K1 (D), eiF2α (E), AKT (F-G), HSP90 (loading control). (H-J) mTORC1 signaling in the muscle was determined by Western blot analysis of phosphorylated S6 (I), phosphorylated 4e-BP1 (K). HSP90 was used as a loading control. (B-G, I-J) Quantification was determined by normalizing phosphorylated protein to normal protein. (A-J) n=5-6 mice/group, quantification was determined using ImageJ by normalizing the phosphorylated protein to the unphosphorylated form. Three-way ANOVA between sex, diet, and gonadectomy with post hoc Šidák’s adjusted test for pairwise comparisons, *p<0.05. p values for the overall effect of sex, diet, and gonadectomy and the interactions represent the significant p values from the three-way ANOVA. Data are represented as mean ±SEM. Abbreviations: C, CTL (21% control protein), L, LP (7% low protein), Cast (castration), Fem (female), Ovx (ovariectomy), Gonad (gonadectomy). Created in BioRender. Knopf, B. (2026) https://BioRender.com/i0gripn

    Article Snippet: Primary antibodies were used at 1:1,000 and were purchased from Cell Signaling Technology (Danvers, MA, USA): p-T389 p70 S6K1 (#9205), p70 S6K1 (#2708), p-Ser240/244 S6 (#2215), S6 (#2217), p-Thr37/46 4E-BP1(#2855), 4E-BP1 (#9452), p-S51 eiF2α (#3398), eiF2α (#5324), p- S473 AKT (#4060), p-T308 AKT (#9275), AKT (#4691), HSP90 (#4877).

    Techniques: Western Blot, Control

    L6 myotubes were treated as described in . Immunoblotting (A) and quantified data for p-AKT ser473 (B) , S6K1 thr389 (C) , S6 ser235/236 (D) and 4E-BP1 thr37/46 (E) are shown. Total proteins for some of the above signals are shown (F, G) . Myotubes in the two treatment groups were incubated in 1µM of puromycin for 30 minutes and sunset analysis was performed (H) . Data are presented as mean ± SEM, n = 3 independent experiments with 3 technical replicates per experiment, * p < 0.05.

    Journal: PLOS One

    Article Title: Myofibrillar protein accumulation but reduced protein synthesis in PDCD4-depleted myotubes

    doi: 10.1371/journal.pone.0345305

    Figure Lengend Snippet: L6 myotubes were treated as described in . Immunoblotting (A) and quantified data for p-AKT ser473 (B) , S6K1 thr389 (C) , S6 ser235/236 (D) and 4E-BP1 thr37/46 (E) are shown. Total proteins for some of the above signals are shown (F, G) . Myotubes in the two treatment groups were incubated in 1µM of puromycin for 30 minutes and sunset analysis was performed (H) . Data are presented as mean ± SEM, n = 3 independent experiments with 3 technical replicates per experiment, * p < 0.05.

    Article Snippet: Antibodies to PDCD4 (#9535), p62 (#5114), beclin-1 (#3738), microtubule-associated proteins 1A/1B light chain 3B (LC3B) (#3868), phosphorylated (p) FoxO3a ser253 (#9466), p-AKT Ser473 (#4060), p-S6 Ser235/236 (#4858), and its kinase S6K1 thr389 (#9234) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Western Blot, Incubation