Review

culture conditions s anginosus (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC culture conditions s anginosus
Culture Conditions S Anginosus, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+anginosus/Streptococcus+anginosus/pm41746808-270-3-7
Average 95 stars, based on 124 article reviews

culture conditions s anginosus - by Bioz Stars, 2026-10

95/100 stars

Images

Laser Capture Microdissection:

Article Title: Cells expressing a chimeric receptor from a modified CD247 locus, related polynucleotides and methods
Article Snippet: .. Examples include a Cas9 molecule of: S. pyogenes (e.g., strain SF370, MGAS10270, MGAS10750, MGAS2096, MGAS315, MGAS5005, MGAS6180, MGAS9429, NZ131 and SSI-1), S. thermophilus (e.g., strain LMD-9), S. pseudoporcinus (e.g., strain SPIN 20026), S. mutans (e.g., strain UA159, NN2025), S. macacae (e.g., strain NCTC11558), S. gallolyticus (e.g., strain UCN34, ATCC BAA-2069), S. equines (e.g., strain ATCC 9812, MGCS 124), S. dysdalactiae (e.g., strain GGS 124), S. bovis (e.g., strain ATCC 700338), S. anginosus (e.g., strain F0211), S. agalactiae (e.g., strain NEM316, A909), Listeria monocytogenes (e.g., strain F6854), Listeria innocua (L. innocua, e.g., strain Clip11262), Enterococcus italicus (e.g., strain DSM 15952), or Enterococcus faecium (e.g., strain 1,231,408). ..

Article Title: Crispr/CAS-related methods and compositions for treating primary open angle glaucoma
Article Snippet: .. Examples include a Cas9 molecule of: S. pyogenes (e.g., strain SF370, MGAS10270, MGAS10750, MGAS2096, MGAS315, MGAS5005, MGAS6180, MGAS9429, NZ131 and SSI-1), S. thermophilus (e.g., strain LMD-9), S. pseudoporcinus (e.g., strain SPIN 20026), S. mutans (e.g., strain UA159, NN2025), S. macacae (e.g., strain NCTC11558), S. gallolyticus (e.g., strain UCN34, ATCC BAA-2069), S. equines (e.g., strain ATCC 9812, MGCS 124), S. dysdalactiae (e.g., strain GGS 124), S. bovis (e.g., strain ATCC 700338), S. anginosus (e.g., strain F0211), S. agalactiae (e.g., strain NEM316, A909), Listeria monocytogenes (e.g., strain F6854), Listeria innocua (L. innocua, e.g., strain Clip11262), Enterococcus italicus (e.g., strain DSM 15952), or Enterococcus faecium (e.g., strain 1,231,408). ..

Bacteria:

Article Title: Salvia coccinea and Apigenin: A Natural Treasure of Lamiaceae in Pharmacological Innovation
Article Snippet: .. Oral bacteria , Broth dilution method , S. mutans (ATCC 25175), S. sanguinis (ATCC 10556), S. sobrinus (ATCC 27607), S. ratti (KCTC 3294), S. criceti (KCTC 3292), S. anginosus (ATCC 31412), S. gordonii (ATCC 10558), A. actinomycetemcomitans (ATCC 43717), F. nucleatum (ATCC 51190), P. intermedia (ATCC 49049), and P. gingivalis (ATCC 33277) , MIC 50 of 25 and 12.5 μg/mL for the respective S. mutans (ATCC 25175), S. sanguinis (ATCC 10556), S. sobrinus (ATCC 27607), S. ratti (KCTC 3294), S. criceti (KCTC 3292), S. anginosus (ATCC 31412), S. gordonii (ATCC 10558), A. actinomycetemcomitans (ATCC 43717), F. nucleatum (ATCC 51190), P. intermedia (ATCC 49049), and P. gingivalis (ATCC 33277) , Cha et al. ( ) . ..

Wound Healing Assay:

Article Title: Intrahepatic Microbial Heterogeneity in Multifocal Hepatocellular Carcinoma and Its Association with Host Genomic and Transcriptomic Alterations
Article Snippet: .. Indeed, the wound-healing assay showed that E. faecalis (isolated from HCC) and S. anginosus (ATCC 33397) both significantly promoted wound closure compared with control cells ( ). ..

Isolation:

Control:

Variant Assay:

Article Title: Streptococcus spp. in oral cancer: host-microbe interactions, mechanistic insights, and diagnostic implications
Article Snippet: .. However, in the case of studies reporting paradoxical phenomena of S. anginosus in oral cancer, research by and both utilized the same variant of S. anginosus (ATCC 33397). ..

Image Search Results

Expression and functionality of CRISPR-Cas system in S. anginosus . (a) RT-PCR performed with 20 ng isolated RNA of S. anginosus SK52 (WT), cas9 deletion (∆ cas9 ), cas9 complementation (∆ cas9:cas9 ) and CRISPR array deletion (∆CRISPR) strain verifying expression of cas9 using primer 13/41. Complete DNase digestion was controlled by conducting PCR without RT step. Controls with genomic DNA of S. anginosus SK52 are indicated by “+” and negative controls without nucleic acid by “-“. The 1 kb plus DNA ladder (Invitrogen) served as molecular weight marker. (b) Confirming the functionality of S. anginosus CRISPR-Cas system by interference with plasmid transformation. Relative transformation efficiencies of created plasmids normalized to the empty vector pAT28 were obtained via competence-based transformation assay. Vectors were designed to contain an endogenous spacer of S. anginosus SK52 CRISPR-Cas system followed by a functional or non-functional PAM sequence, resulting in the CRISPR-targeted plasmid pAT28_SP1_TGG and the non-targeted plasmid pAT28_SP1_AAA. Mean values and standard deviations of six independent experiments are shown. Mann-Whitney-U test was performed to illustrate significant differences as indicated (** p < 0.01).

Journal: Virulence

Article Title: The stress of carrying CRISPR-Cas

doi: 10.1080/21505594.2025.2541701

Figure Lengend Snippet: Expression and functionality of CRISPR-Cas system in S. anginosus . (a) RT-PCR performed with 20 ng isolated RNA of S. anginosus SK52 (WT), cas9 deletion (∆ cas9 ), cas9 complementation (∆ cas9:cas9 ) and CRISPR array deletion (∆CRISPR) strain verifying expression of cas9 using primer 13/41. Complete DNase digestion was controlled by conducting PCR without RT step. Controls with genomic DNA of S. anginosus SK52 are indicated by “+” and negative controls without nucleic acid by “-“. The 1 kb plus DNA ladder (Invitrogen) served as molecular weight marker. (b) Confirming the functionality of S. anginosus CRISPR-Cas system by interference with plasmid transformation. Relative transformation efficiencies of created plasmids normalized to the empty vector pAT28 were obtained via competence-based transformation assay. Vectors were designed to contain an endogenous spacer of S. anginosus SK52 CRISPR-Cas system followed by a functional or non-functional PAM sequence, resulting in the CRISPR-targeted plasmid pAT28_SP1_TGG and the non-targeted plasmid pAT28_SP1_AAA. Mean values and standard deviations of six independent experiments are shown. Mann-Whitney-U test was performed to illustrate significant differences as indicated (** p < 0.01).

Article Snippet: Streptococcus anginosus SK 52 , S. anginosus type strain, ATCC 33397, Hly+ , ATCC.

Techniques: Expressing, CRISPR, Reverse Transcription Polymerase Chain Reaction, Isolation, Molecular Weight, Marker, Plasmid Preparation, Transformation Assay, Functional Assay, Sequencing, MANN-WHITNEY

Effects of UV irradiation on viability of S. anginosus SK52 and mutant strains ∆ cas9 , ∆ cas9:cas9 and ∆CRISPR. (a) Strains on this agar plate were exposed to UV irradiation for 43 s − 96 s on THY agar followed by 24 h of growth at 37°C. Shown is one representative plate out of five independently conducted experiments. (b) Results of (a) are represented as lethal irradiation time from which less than five CFUs could grow. (c) S. anginosus SK52, ∆ cas9 and clinical isolates naturally carrying (yellow n = 5) or lacking (orange n = 4) a type II-A CRISPR-Cas system were exposed to UV irradiation on THY agar followed by 24 h of growth at 37°C. Results are presented as percentage of the lethal irradiation time of strain SK52. (b) and (c) shown is the mean ± standard deviation of five independent experiments. Significant differences were calculated with Mann-Whitney-U test (** p < 0.01).

Journal: Virulence

Article Title: The stress of carrying CRISPR-Cas

doi: 10.1080/21505594.2025.2541701

Figure Lengend Snippet: Effects of UV irradiation on viability of S. anginosus SK52 and mutant strains ∆ cas9 , ∆ cas9:cas9 and ∆CRISPR. (a) Strains on this agar plate were exposed to UV irradiation for 43 s − 96 s on THY agar followed by 24 h of growth at 37°C. Shown is one representative plate out of five independently conducted experiments. (b) Results of (a) are represented as lethal irradiation time from which less than five CFUs could grow. (c) S. anginosus SK52, ∆ cas9 and clinical isolates naturally carrying (yellow n = 5) or lacking (orange n = 4) a type II-A CRISPR-Cas system were exposed to UV irradiation on THY agar followed by 24 h of growth at 37°C. Results are presented as percentage of the lethal irradiation time of strain SK52. (b) and (c) shown is the mean ± standard deviation of five independent experiments. Significant differences were calculated with Mann-Whitney-U test (** p < 0.01).

Article Snippet: Streptococcus anginosus SK 52 , S. anginosus type strain, ATCC 33397, Hly+ , ATCC.

Techniques: Irradiation, Mutagenesis, CRISPR, Standard Deviation, MANN-WHITNEY

CRISPR-Cas related stress response to high temperature and oxidative stress. (a) Survival of S. anginosus SK52 and mutant strains after exposure to 50°C temperature stress for 10 min −120 min. Results represent mean CFU and standard deviations of five independent experiments conducted with the S. anginosus SK52 and mutant strains. Survival was determined as percentage of viable counts of heat-treated bacteria compared to the untreated bacteria. (b) Effect of oxidative stress to the survival of S. anginosus SK52 and mutant strains. S. anginosus SK52, as well as ∆ cas9 , ∆ cas9:cas9 and ∆CRISPR strains were subjected to 2 mM −3.5 mM hydrogen peroxide (H 2 O 2 ) for 24 h. Results were obtained during four independent experiments and represent mean values and standard deviations. Differences were statistically significant (* p < 0.05, ** p < 0.01; Mann-Whitney-U test), unless otherwise indicated it refers to wild-type strain.

Journal: Virulence

Article Title: The stress of carrying CRISPR-Cas

doi: 10.1080/21505594.2025.2541701

Figure Lengend Snippet: CRISPR-Cas related stress response to high temperature and oxidative stress. (a) Survival of S. anginosus SK52 and mutant strains after exposure to 50°C temperature stress for 10 min −120 min. Results represent mean CFU and standard deviations of five independent experiments conducted with the S. anginosus SK52 and mutant strains. Survival was determined as percentage of viable counts of heat-treated bacteria compared to the untreated bacteria. (b) Effect of oxidative stress to the survival of S. anginosus SK52 and mutant strains. S. anginosus SK52, as well as ∆ cas9 , ∆ cas9:cas9 and ∆CRISPR strains were subjected to 2 mM −3.5 mM hydrogen peroxide (H 2 O 2 ) for 24 h. Results were obtained during four independent experiments and represent mean values and standard deviations. Differences were statistically significant (* p < 0.05, ** p < 0.01; Mann-Whitney-U test), unless otherwise indicated it refers to wild-type strain.

Article Snippet: Streptococcus anginosus SK 52 , S. anginosus type strain, ATCC 33397, Hly+ , ATCC.

Techniques: CRISPR, Mutagenesis, Bacteria, MANN-WHITNEY

Growth behaviour of S. anginosus influenced by Cas9. (a) Differences in colony size of S. anginosus SK52 (WT), cas9 deletion mutant (∆ cas9 ), cas9 complementation mutant (∆ cas9:cas9 ) and CRISPR array deletion mutant (∆CRISPR). 10 µl droplets of indicated strains were incubated for 24 h on THY agar. (b) Growth of S. anginosus SK52 wild-type and mutant strains over time. Each point represents the average of five independent optical density values per sample including standard deviation. Differences were statistically significant (* p < 0.05, ** p < 0.01; Mann-Whitney-U test). When indicated on left side of the ∆ cas9 growth curve it refers to wild-type strain and on right side it refers to ∆ cas9:cas9 .

Journal: Virulence

Article Title: The stress of carrying CRISPR-Cas

doi: 10.1080/21505594.2025.2541701

Figure Lengend Snippet: Growth behaviour of S. anginosus influenced by Cas9. (a) Differences in colony size of S. anginosus SK52 (WT), cas9 deletion mutant (∆ cas9 ), cas9 complementation mutant (∆ cas9:cas9 ) and CRISPR array deletion mutant (∆CRISPR). 10 µl droplets of indicated strains were incubated for 24 h on THY agar. (b) Growth of S. anginosus SK52 wild-type and mutant strains over time. Each point represents the average of five independent optical density values per sample including standard deviation. Differences were statistically significant (* p < 0.05, ** p < 0.01; Mann-Whitney-U test). When indicated on left side of the ∆ cas9 growth curve it refers to wild-type strain and on right side it refers to ∆ cas9:cas9 .

Article Snippet: Streptococcus anginosus SK 52 , S. anginosus type strain, ATCC 33397, Hly+ , ATCC.

Techniques: Mutagenesis, CRISPR, Incubation, Standard Deviation, MANN-WHITNEY

Metabolic activity related to the expression of cas9 with and without environmental stress. (a) Effect of 50°C temperature stress on the metabolic activity of S. anginosus SK52 and mutant strains within 4 h in a resazurin assay. (b) Measurement of the metabolic activity of S. anginosus SK52 and mutant strains within 2 h in a resazurin assay. Results represent the mean absorbance ratio 572 nm/600 nm and standard deviations of five independent experiments conducted with the S. anginosus SK52 and mutant strains. Differences were statistically significant (* p < 0.05, ** p < 0.01; Mann-Whitney-U test), unless otherwise indicated it refers to wild-type strain.

Journal: Virulence

Article Title: The stress of carrying CRISPR-Cas

doi: 10.1080/21505594.2025.2541701

Figure Lengend Snippet: Metabolic activity related to the expression of cas9 with and without environmental stress. (a) Effect of 50°C temperature stress on the metabolic activity of S. anginosus SK52 and mutant strains within 4 h in a resazurin assay. (b) Measurement of the metabolic activity of S. anginosus SK52 and mutant strains within 2 h in a resazurin assay. Results represent the mean absorbance ratio 572 nm/600 nm and standard deviations of five independent experiments conducted with the S. anginosus SK52 and mutant strains. Differences were statistically significant (* p < 0.05, ** p < 0.01; Mann-Whitney-U test), unless otherwise indicated it refers to wild-type strain.

Article Snippet: Streptococcus anginosus SK 52 , S. anginosus type strain, ATCC 33397, Hly+ , ATCC.

Techniques: Activity Assay, Expressing, Mutagenesis, Resazurin Assay, MANN-WHITNEY

Review

culture conditions s anginosus (ATCC)

Structured Review

Images

Related Articles

Laser Capture Microdissection:

Bacteria:

Wound Healing Assay:

Isolation:

Control:

Variant Assay:

Similar Products

Image Search Results