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rtp1s  (Matsunami Glass)

 
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    Matsunami Glass rtp1s
    Rtp1s, supplied by Matsunami Glass, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rtp1s/rtp1s/10__1134_slash_s0022093023070013-1832-23-14
    Average 90 stars, based on 1 article reviews
    rtp1s - by Bioz Stars, 2026-11
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    Related Articles

    Transfection:

    Article Title: Olfactory Marker Protein Expression Is an Indicator of Olfactory Receptor-Associated Events in Non-Olfactory Tissues
    Article Snippet: .. HEK 293 cells were seeded onto 22 mm coverslips coated with poly-D-lysine and transfected with OR constructs containing accessory proteins RTP1S (a gift from Hiroaki Matsunami), Ric8B, and G olf (a gift from Dr. Bettina Malnic at University of São Paulo, Brazil) to assess universal effects for plasma membrane expression. .. Briefly, transiently transfected cells were incubated on ice to block endocytosis, washed with 1× PBS, fixed with ice-cold methanol/acetone (1:1, v/v), and then exposed to mouse anti-Rho antibody and rabbit anti-OR antibodies (OR51E1, olfr1496, olfr1386, and olfr544) in PBS with 5% normal horse serum (NHS, Jackson ImmunoResearch) overnight at room temperature as described in .

    Article Title: Oxygen regulation of breathing through an olfactory receptor activated by lactate.
    Article Snippet: .. On the next day, cells were transfected with RTP1S, Gα15-olf (gifts from H. Matsunami), pCMV6-Ric8b (Origene), pCRE-Luc (Agilent), and pSV40-RL (Promega) plasmids and either pCI-Rho-Olfr78 or pCI (Promega). ..

    Article Title: The smelling of Hedione results in sex-differentiated human brain activity.
    Article Snippet: NeuroImage xxx (2015) xxx–xxx YNIMG-12070; No. of pages: 9; 4C: 6, 5 Contents lists available at ScienceDirect NeuroImage j ourna l homepage: www.e lsev ie r .com/ locate /yn img The smelling of Hedione results in sex-differentiated human brain activity I. Wallrabenstein a,⁎,1, J. Gerber b,1, S. Rasche a,1, I. Croy c, S. Kurtenbach a, T. Hummel c, H. Hatt a a Department of Cell Physiology, Ruhr University Bochum, Universitätsstr.. 150, 44801 Bochum, Germany b Department of Neuroradiology, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Fetscherstr.. 74, 01307 Dresden, Germany c Department of Otorhinolaryngology, Smell and Taste Clinic, Technische Universität Dresden, Fetscherstr.

    Construct:

    Article Title: Olfactory Marker Protein Expression Is an Indicator of Olfactory Receptor-Associated Events in Non-Olfactory Tissues
    Article Snippet: .. HEK 293 cells were seeded onto 22 mm coverslips coated with poly-D-lysine and transfected with OR constructs containing accessory proteins RTP1S (a gift from Hiroaki Matsunami), Ric8B, and G olf (a gift from Dr. Bettina Malnic at University of São Paulo, Brazil) to assess universal effects for plasma membrane expression. .. Briefly, transiently transfected cells were incubated on ice to block endocytosis, washed with 1× PBS, fixed with ice-cold methanol/acetone (1:1, v/v), and then exposed to mouse anti-Rho antibody and rabbit anti-OR antibodies (OR51E1, olfr1496, olfr1386, and olfr544) in PBS with 5% normal horse serum (NHS, Jackson ImmunoResearch) overnight at room temperature as described in .

    Clinical Proteomics:

    Article Title: Olfactory Marker Protein Expression Is an Indicator of Olfactory Receptor-Associated Events in Non-Olfactory Tissues
    Article Snippet: .. HEK 293 cells were seeded onto 22 mm coverslips coated with poly-D-lysine and transfected with OR constructs containing accessory proteins RTP1S (a gift from Hiroaki Matsunami), Ric8B, and G olf (a gift from Dr. Bettina Malnic at University of São Paulo, Brazil) to assess universal effects for plasma membrane expression. .. Briefly, transiently transfected cells were incubated on ice to block endocytosis, washed with 1× PBS, fixed with ice-cold methanol/acetone (1:1, v/v), and then exposed to mouse anti-Rho antibody and rabbit anti-OR antibodies (OR51E1, olfr1496, olfr1386, and olfr544) in PBS with 5% normal horse serum (NHS, Jackson ImmunoResearch) overnight at room temperature as described in .

    Membrane:

    Article Title: Olfactory Marker Protein Expression Is an Indicator of Olfactory Receptor-Associated Events in Non-Olfactory Tissues
    Article Snippet: .. HEK 293 cells were seeded onto 22 mm coverslips coated with poly-D-lysine and transfected with OR constructs containing accessory proteins RTP1S (a gift from Hiroaki Matsunami), Ric8B, and G olf (a gift from Dr. Bettina Malnic at University of São Paulo, Brazil) to assess universal effects for plasma membrane expression. .. Briefly, transiently transfected cells were incubated on ice to block endocytosis, washed with 1× PBS, fixed with ice-cold methanol/acetone (1:1, v/v), and then exposed to mouse anti-Rho antibody and rabbit anti-OR antibodies (OR51E1, olfr1496, olfr1386, and olfr544) in PBS with 5% normal horse serum (NHS, Jackson ImmunoResearch) overnight at room temperature as described in .

    Expressing:

    Article Title: Olfactory Marker Protein Expression Is an Indicator of Olfactory Receptor-Associated Events in Non-Olfactory Tissues
    Article Snippet: .. HEK 293 cells were seeded onto 22 mm coverslips coated with poly-D-lysine and transfected with OR constructs containing accessory proteins RTP1S (a gift from Hiroaki Matsunami), Ric8B, and G olf (a gift from Dr. Bettina Malnic at University of São Paulo, Brazil) to assess universal effects for plasma membrane expression. .. Briefly, transiently transfected cells were incubated on ice to block endocytosis, washed with 1× PBS, fixed with ice-cold methanol/acetone (1:1, v/v), and then exposed to mouse anti-Rho antibody and rabbit anti-OR antibodies (OR51E1, olfr1496, olfr1386, and olfr544) in PBS with 5% normal horse serum (NHS, Jackson ImmunoResearch) overnight at room temperature as described in .

    Article Title: The smelling of Hedione results in sex-differentiated human brain activity.
    Article Snippet: NeuroImage xxx (2015) xxx–xxx YNIMG-12070; No. of pages: 9; 4C: 6, 5 Contents lists available at ScienceDirect NeuroImage j ourna l homepage: www.e lsev ie r .com/ locate /yn img The smelling of Hedione results in sex-differentiated human brain activity I. Wallrabenstein a,⁎,1, J. Gerber b,1, S. Rasche a,1, I. Croy c, S. Kurtenbach a, T. Hummel c, H. Hatt a a Department of Cell Physiology, Ruhr University Bochum, Universitätsstr.. 150, 44801 Bochum, Germany b Department of Neuroradiology, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Fetscherstr.. 74, 01307 Dresden, Germany c Department of Otorhinolaryngology, Smell and Taste Clinic, Technische Universität Dresden, Fetscherstr.

    Article Title: The mouse receptor transporting protein RTP1S and the fly SNMP1 support the functional expression of the Drosophila odorant coreceptor Orco in mammalian culture cells.
    Article Snippet: Background Functional expression of vertebrate and insect odorant receptors (ORs) in mammalian culture cells is hampered by an incorrect trafficking of these proteins to the plasma membrane.. Receptor transporting proteins (RTPs) have been found to enhance the activity of transfected mammalian ORs in several heterologous systems.. New Methods We co-transfected the Drosophila olfactory coreceptor (Orco) in HEK293 cells with a truncated form of the mouse RTP1 (RTP1S) or with the Drosophila sensory neuron membrane protein 1 (SNMP1), which is required for the detection of the pheromone cis-vaccenyl acetate and was shown to be apposed to Orco within the functional receptor unit.

    Plasmid Preparation:

    Article Title: Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
    Article Snippet: phase contrast microscope , Leica , 090-131.001 , phase contrast microscope with x4, x10, x20 objectives. .. RTP1S , H. Matsunami lab , - , 100 ng/μL plasmid - store at 4°C. ..

    other:

    Article Title: Identification and Characterization of Proteins That Are Involved in RTP1S-Dependent Transport of Olfactory Receptors.
    Article Snippet: Citation: Inoue, R.; Fukutani, Y.; Niwa, T.; Matsunami, H.; Yohda, M. Identification and Characterization of Proteins That Are Involved in RTP1S-Dependent Transport of Olfactory Receptors.

    Article Title: Axonal Odorant Receptors Mediate Axon Targeting
    Article Snippet: RTP1S , H. Matsunami , Duke University.



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    Image Search Results


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    Journal: Nature Communications

    Article Title: AAV-delivered muscone-induced transgene system for treating chronic diseases in mice via inhalation

    doi: 10.1038/s41467-024-45383-z

    Figure Lengend Snippet: a Schematic representing the genetic configuration of the AAV vector used for AAV MUSE iteration. b Schematic representation of the experimental procedure for AAV MUSE -mediated transgene expression in mouse lungs. c AAV MUSE -mediated luciferase expression for 20 weeks in mouse lungs. Eight-week-old female BALB/c mice were transduced with an AAV MUSE iteration comprising AAV2/lung-pWX126 (ITR-P SV40 -MOR215-1-pA-ITR, 5 × 10 11 vg) and AAV2/6-pWX342 (ITR-P SV40 -RTP1S::pA-luciferase-P CRE -ITR, 5 × 10 11 vg) via tail vein. Two weeks after the AAV injection, AAV MUSE -transduced mice were exposed to nebulized muscone for 4 h once every six weeks or exposed to a vehicle (a mixture of castor oil and ddH 2 O), and bioluminescence imaging was performed using an in vivo imaging system every 6 weeks. d Quantification of luciferase expression in the lung based on the bioluminescence IVIS imaging shown in ( c ). Data in ( d ) are presented as means ± SEM ( n = 4 mice). ** P < 0.01, *** P < 0.001. P values were obtained from two-tailed unpaired t tests. Source data are provided as a file.

    Article Snippet: AAV (serotype 2/6) carrying pWX342 (ITR-P SV40 -RTP1S::pA-luciferase-P CRE -ITR) or pWX345 (ITR-P SV40 -RTP1S::pA-ΔmIL-4-P CRE -ITR) and AAV (serotype 2/lung) carrying pWX126, pWX127, or pWX158 were produced by Shanghai OBiO Technology.

    Techniques: Plasmid Preparation, Expressing, Luciferase, Transduction, Injection, Imaging, In Vivo Imaging, Two Tailed Test

    Endpoint measurement of the binding affinity of odor molecules to OR expressed on heterologous cells. To promote OR expression on the surface of heterologous cells, a signal peptide (Rho-tag) is added to the N -terminus and the OR chaperones RTPs (RTP1, RTP1S, RTP2) and REEP1 and Gα protein chaperone Ric-8B are coexpressed. Binding of the odor molecule causes OR to activate adenylate cyclase (AC) via trimeric G proteins to produce cAMP. The amount of cAMP is assayed by endpoint measurement of chemiluminescence using GloSensor TM and is determined as the binding affinity between the odor molecules and OR.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Human Olfactory Receptor Sensor for Odor Reconstitution

    doi: 10.3390/s23136164

    Figure Lengend Snippet: Endpoint measurement of the binding affinity of odor molecules to OR expressed on heterologous cells. To promote OR expression on the surface of heterologous cells, a signal peptide (Rho-tag) is added to the N -terminus and the OR chaperones RTPs (RTP1, RTP1S, RTP2) and REEP1 and Gα protein chaperone Ric-8B are coexpressed. Binding of the odor molecule causes OR to activate adenylate cyclase (AC) via trimeric G proteins to produce cAMP. The amount of cAMP is assayed by endpoint measurement of chemiluminescence using GloSensor TM and is determined as the binding affinity between the odor molecules and OR.

    Article Snippet: Matsunami et al. found that human embryonic kidney-derived HEK293 cells introduced with the chaperones RTP1 (receptor-transporting protein 1), RTP2, and REEP1 (receptor expression-enhancing protein 1), also called Hana3A cells, greatly improved cell surface expression of ORs with an N -terminal Rho-tag (rhodopsin-derived signal peptide) [ ] ( ).

    Techniques: Binding Assay, Expressing