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rsv b  (ATCC)


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    Structured Review

    ATCC rsv b
    (A) All age-group hospitalization rates in Connecticut from 2021 – 2025, based on data <t>from</t> <t>RSV-NET</t> and colored by sequence availability. (B) Summary of local Connecticut clades <t>(RSV-B</t> = 43, RSV-A = 33). Persistence bars are colored by subtype (RSV-A, pink; RSV-B, blue). Each vertical dotted line represents the start of a calendar year mark; RSV epidemic seasons are indicated by a light grey background. (C) Histogram of observed persistence duration; vertical dashed lines represent the median duration of lineage persistence.
    Rsv B, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rsv+b/Human+respiratory+syncytial+virus/med_rxiv__64898__2026__05__07__26350887-127-11-12
    Average 97 stars, based on 450 article reviews
    rsv b - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Genomic surveillance of a deeply sampled local population reveals age-specific drivers of RSV transmission"

    Article Title: Genomic surveillance of a deeply sampled local population reveals age-specific drivers of RSV transmission

    Journal: medRxiv

    doi: 10.64898/2026.05.07.26350887

    (A) All age-group hospitalization rates in Connecticut from 2021 – 2025, based on data from RSV-NET and colored by sequence availability. (B) Summary of local Connecticut clades (RSV-B = 43, RSV-A = 33). Persistence bars are colored by subtype (RSV-A, pink; RSV-B, blue). Each vertical dotted line represents the start of a calendar year mark; RSV epidemic seasons are indicated by a light grey background. (C) Histogram of observed persistence duration; vertical dashed lines represent the median duration of lineage persistence.
    Figure Legend Snippet: (A) All age-group hospitalization rates in Connecticut from 2021 – 2025, based on data from RSV-NET and colored by sequence availability. (B) Summary of local Connecticut clades (RSV-B = 43, RSV-A = 33). Persistence bars are colored by subtype (RSV-A, pink; RSV-B, blue). Each vertical dotted line represents the start of a calendar year mark; RSV epidemic seasons are indicated by a light grey background. (C) Histogram of observed persistence duration; vertical dashed lines represent the median duration of lineage persistence.

    Techniques Used: Sequencing

    Maximum clade credibility (MCC) trees illustrating seven potential trans-seasonal persistent clades (three RSV-A and four RSV-B). Clades are defined by tips sampled across more than one RSV season from a single inferred introduction event. Tips are colored according to the age group from which the sequence was sampled. Internal branches are colored by the inferred ancestral age group, which is derived from the larger global phylogenetic tree.
    Figure Legend Snippet: Maximum clade credibility (MCC) trees illustrating seven potential trans-seasonal persistent clades (three RSV-A and four RSV-B). Clades are defined by tips sampled across more than one RSV season from a single inferred introduction event. Tips are colored according to the age group from which the sequence was sampled. Internal branches are colored by the inferred ancestral age group, which is derived from the larger global phylogenetic tree.

    Techniques Used: Sequencing, Derivative Assay

    Related Articles

    Quantitative RT-PCR:

    Article Title: Reliability of RSV Antigen Diagnostic Tests in the Presence of Nirsevimab
    Article Snippet: One-step reverse transcriptase quantitative PCR (RT-qPCR) was subsequently conducted utilizing AgPATH-ID One-Step RT-PCR Reagents (4387424, Applied Biosystems, Foster City, CA) and previously described RSV-specific primer and probe sequences.12 Reactions were run in duplicate with a total volume of 25 μL comprising 12.5 μL of 2X RT-PCR Buffer, 1 μL 25X RT-PCR Enzyme Mix, 5 μL extracted vRNA, and 500 nM of each primer and 150 nM fluorescein amidite-labeled probe. .. RT-qPCR was conducted on an Applied Biosystems (Foster City, CA) QuantStudio 7 Flex with thermal cycler conditions of 50°C for 2 minutes, 60°C for 20 minutes, 95°C for 5 minutes and 45 cycles of 94°C for 20 seconds and 60°C for 1 minute.12 Quantitative genomic RNA from RSV A (ATCC VR-1540DQ) and RSV B (ATCC VR-1580DQ) was used to generate a standard curve for the RT-qPCR assay, allowing the observed cycle threshold values from the specimen pools to be converted into genome copies/mL using linear regression. ..

    Multiplex Assay:

    Article Title: Genomic surveillance of a deeply sampled local population reveals age-specific drivers of RSV transmission
    Article Snippet: .. We tested our primer set with commercial RSV-A (ATCC VR-1540) / RSV-B (ATCC VR-3381) standards, both individually and as a multiplex, and then validated with 182 mixed-genotype (A and B) clinical samples to confirm that we could sequence without the need for prior subtyping. ..

    Sequencing:

    Article Title: Genomic surveillance of a deeply sampled local population reveals age-specific drivers of RSV transmission
    Article Snippet: .. We tested our primer set with commercial RSV-A (ATCC VR-1540) / RSV-B (ATCC VR-3381) standards, both individually and as a multiplex, and then validated with 182 mixed-genotype (A and B) clinical samples to confirm that we could sequence without the need for prior subtyping. ..

    Article Title: Development of a reverse genetic system for mononegaviruses using a circular polymerase extension reaction.
    Article Snippet: Reverse genetic systems are commonly used to study viruses; the ability to rapidly generate recombinant viruses is critical for studying the functions of viral genes and for the development of new interventions, such as antivirals or vaccines.. Reverse genetic methods for mononegaviruses, viruses with non-segmented negative-strand RNA genomes, commonly incorporate a full-length viral genome cDNA into a bacterial artificial chromosomes (BACs) or plasmid DNA.. However, the large size of mononegavirus genomes makes their manipulation challenging.

    Incubation:

    Article Title: Metavac-RSV mucosal bivalent vaccine candidate protects cotton rats against pneumoviruses and is produced using serum-free cell culture in bioreactor
    Article Snippet: .. Diluted samples were incubated with 25-50 PFU of RSV A (RSV A2, lot #102313 SSM), RSV B (RSV B 18537, ATCC VR-1580, lot #032417 SSM), or HMPV A (HMPV TN/94-49/A2, lot #030116 SSM) for 1 h at room temperature and inoculated in duplicates onto confluent HEp-2 for RSV, or LLC-MK2 for HMPV. ..

    Isolation:

    Article Title: Neutralizing Antibody Response to the AreXvy Respiratory Syncytial Virus Vaccine in Lung Transplant Recipients: Assessment Against Reference and Seasonal Strains.
    Article Snippet: .. Viral Isolation of RSV Variants RSV A (ATCC VR-26, 1956) and RSV B (ATCC VR-955, 1977) lineages, along with seasonal RSV A (R17535/Israel) and B (R15474/Israel) strains, were propagated in vitro and used to evaluate antibody response. ..

    In Vitro:

    Article Title: Neutralizing Antibody Response to the AreXvy Respiratory Syncytial Virus Vaccine in Lung Transplant Recipients: Assessment Against Reference and Seasonal Strains.
    Article Snippet: .. Viral Isolation of RSV Variants RSV A (ATCC VR-26, 1956) and RSV B (ATCC VR-955, 1977) lineages, along with seasonal RSV A (R17535/Israel) and B (R15474/Israel) strains, were propagated in vitro and used to evaluate antibody response. ..

    Article Title: Neutralizing Antibody Response to the AreXvy Respiratory Syncytial Virus Vaccine in Lung Transplant Recipients: Assessment Against Reference and Seasonal Strains
    Article Snippet: .. RSV A (ATCC VR-26, 1956) and RSV B (ATCC VR-955, 1977) lineages, along with seasonal RSV A (R17535/Israel) and B (R15474/Israel) strains, were propagated in vitro and used to evaluate antibody response. ..

    Virus:

    Article Title: Development of a reverse genetic system for mononegaviruses using a circular polymerase extension reaction.
    Article Snippet: Reverse genetic systems are commonly used to study viruses; the ability to rapidly generate recombinant viruses is critical for studying the functions of viral genes and for the development of new interventions, such as antivirals or vaccines.. Reverse genetic methods for mononegaviruses, viruses with non-segmented negative-strand RNA genomes, commonly incorporate a full-length viral genome cDNA into a bacterial artificial chromosomes (BACs) or plasmid DNA.. However, the large size of mononegavirus genomes makes their manipulation challenging.

    Neutralization:

    Article Title: Mucosal immunization with an adenoviral vector expressing a prefusogenic F protein protects the upper and lower respiratory tracts of cotton rats against live respiratory syncytial virus challenge
    Article Snippet: The RSV Long strain (ATCC, VR-26) and HEp-2 cells (ATCC CCL-23) used for neutralization assays in the mouse study were obtained from the National Institute for Viral Disease Control and Prevention, China CDC. .. Neutralization assays in cotton rats were performed using the following strains: RSV A2 (ATCC VR-1540), RSV B (strain 18537; ATCC VR-1580PQ), and RSV Long (ATCC VR-26). .. These viruses were propagated in human epithelial type 2 (HEp-2) cells (ATCC CCL-23) by Sigmovir Biosystems (Rockville, MD).



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    rsv b  (ATCC)
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    (A) All age-group hospitalization rates in Connecticut from 2021 – 2025, based on data <t>from</t> <t>RSV-NET</t> and colored by sequence availability. (B) Summary of local Connecticut clades <t>(RSV-B</t> = 43, RSV-A = 33). Persistence bars are colored by subtype (RSV-A, pink; RSV-B, blue). Each vertical dotted line represents the start of a calendar year mark; RSV epidemic seasons are indicated by a light grey background. (C) Histogram of observed persistence duration; vertical dashed lines represent the median duration of lineage persistence.
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    (A) All age-group hospitalization rates in Connecticut from 2021 – 2025, based on data <t>from</t> <t>RSV-NET</t> and colored by sequence availability. (B) Summary of local Connecticut clades <t>(RSV-B</t> = 43, RSV-A = 33). Persistence bars are colored by subtype (RSV-A, pink; RSV-B, blue). Each vertical dotted line represents the start of a calendar year mark; RSV epidemic seasons are indicated by a light grey background. (C) Histogram of observed persistence duration; vertical dashed lines represent the median duration of lineage persistence.
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    (A) All age-group hospitalization rates in Connecticut from 2021 – 2025, based on data <t>from</t> <t>RSV-NET</t> and colored by sequence availability. (B) Summary of local Connecticut clades <t>(RSV-B</t> = 43, RSV-A = 33). Persistence bars are colored by subtype (RSV-A, pink; RSV-B, blue). Each vertical dotted line represents the start of a calendar year mark; RSV epidemic seasons are indicated by a light grey background. (C) Histogram of observed persistence duration; vertical dashed lines represent the median duration of lineage persistence.
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    (A) All age-group hospitalization rates in Connecticut from 2021 – 2025, based on data <t>from</t> <t>RSV-NET</t> and colored by sequence availability. (B) Summary of local Connecticut clades <t>(RSV-B</t> = 43, RSV-A = 33). Persistence bars are colored by subtype (RSV-A, pink; RSV-B, blue). Each vertical dotted line represents the start of a calendar year mark; RSV epidemic seasons are indicated by a light grey background. (C) Histogram of observed persistence duration; vertical dashed lines represent the median duration of lineage persistence.
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    (A) Longitudinal trajectories of serum IgG (top row) and mucosal IgA (bottom row) antibody responses against <t>four</t> <t>RSV-B</t> proteins (PreF, PostF, G, NP) over time. Individual participant trajectories are shown as thin lines with low opacity, colored by infection status: not infected (black), sero-detected infections (orange), and PCR-confirmed infections (green). (B) Mean fold-change in antibody titres between the first bleed (pre-epidemic baseline) and second bleed (post-epidemic) for serum IgG and mucosal IgA responses to RSV-B proteins. Bars represent mean fold-change (log10 scale) stratified by infection status, with error bars indicating standard error.
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    (A) Longitudinal trajectories of serum IgG (top row) and mucosal IgA (bottom row) antibody responses against <t>four</t> <t>RSV-B</t> proteins (PreF, PostF, G, NP) over time. Individual participant trajectories are shown as thin lines with low opacity, colored by infection status: not infected (black), sero-detected infections (orange), and PCR-confirmed infections (green). (B) Mean fold-change in antibody titres between the first bleed (pre-epidemic baseline) and second bleed (post-epidemic) for serum IgG and mucosal IgA responses to RSV-B proteins. Bars represent mean fold-change (log10 scale) stratified by infection status, with error bars indicating standard error.
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    (A) Longitudinal trajectories of serum IgG (top row) and mucosal IgA (bottom row) antibody responses against <t>four</t> <t>RSV-B</t> proteins (PreF, PostF, G, NP) over time. Individual participant trajectories are shown as thin lines with low opacity, colored by infection status: not infected (black), sero-detected infections (orange), and PCR-confirmed infections (green). (B) Mean fold-change in antibody titres between the first bleed (pre-epidemic baseline) and second bleed (post-epidemic) for serum IgG and mucosal IgA responses to RSV-B proteins. Bars represent mean fold-change (log10 scale) stratified by infection status, with error bars indicating standard error.
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    Image Search Results


    (A) All age-group hospitalization rates in Connecticut from 2021 – 2025, based on data from RSV-NET and colored by sequence availability. (B) Summary of local Connecticut clades (RSV-B = 43, RSV-A = 33). Persistence bars are colored by subtype (RSV-A, pink; RSV-B, blue). Each vertical dotted line represents the start of a calendar year mark; RSV epidemic seasons are indicated by a light grey background. (C) Histogram of observed persistence duration; vertical dashed lines represent the median duration of lineage persistence.

    Journal: medRxiv

    Article Title: Genomic surveillance of a deeply sampled local population reveals age-specific drivers of RSV transmission

    doi: 10.64898/2026.05.07.26350887

    Figure Lengend Snippet: (A) All age-group hospitalization rates in Connecticut from 2021 – 2025, based on data from RSV-NET and colored by sequence availability. (B) Summary of local Connecticut clades (RSV-B = 43, RSV-A = 33). Persistence bars are colored by subtype (RSV-A, pink; RSV-B, blue). Each vertical dotted line represents the start of a calendar year mark; RSV epidemic seasons are indicated by a light grey background. (C) Histogram of observed persistence duration; vertical dashed lines represent the median duration of lineage persistence.

    Article Snippet: We tested our primer set with commercial RSV-A (ATCC VR-1540) / RSV-B (ATCC VR-3381) standards, both individually and as a multiplex, and then validated with 182 mixed-genotype (A and B) clinical samples to confirm that we could sequence without the need for prior subtyping.

    Techniques: Sequencing

    Maximum clade credibility (MCC) trees illustrating seven potential trans-seasonal persistent clades (three RSV-A and four RSV-B). Clades are defined by tips sampled across more than one RSV season from a single inferred introduction event. Tips are colored according to the age group from which the sequence was sampled. Internal branches are colored by the inferred ancestral age group, which is derived from the larger global phylogenetic tree.

    Journal: medRxiv

    Article Title: Genomic surveillance of a deeply sampled local population reveals age-specific drivers of RSV transmission

    doi: 10.64898/2026.05.07.26350887

    Figure Lengend Snippet: Maximum clade credibility (MCC) trees illustrating seven potential trans-seasonal persistent clades (three RSV-A and four RSV-B). Clades are defined by tips sampled across more than one RSV season from a single inferred introduction event. Tips are colored according to the age group from which the sequence was sampled. Internal branches are colored by the inferred ancestral age group, which is derived from the larger global phylogenetic tree.

    Article Snippet: We tested our primer set with commercial RSV-A (ATCC VR-1540) / RSV-B (ATCC VR-3381) standards, both individually and as a multiplex, and then validated with 182 mixed-genotype (A and B) clinical samples to confirm that we could sequence without the need for prior subtyping.

    Techniques: Sequencing, Derivative Assay

    (A) Longitudinal trajectories of serum IgG (top row) and mucosal IgA (bottom row) antibody responses against four RSV-B proteins (PreF, PostF, G, NP) over time. Individual participant trajectories are shown as thin lines with low opacity, colored by infection status: not infected (black), sero-detected infections (orange), and PCR-confirmed infections (green). (B) Mean fold-change in antibody titres between the first bleed (pre-epidemic baseline) and second bleed (post-epidemic) for serum IgG and mucosal IgA responses to RSV-B proteins. Bars represent mean fold-change (log10 scale) stratified by infection status, with error bars indicating standard error.

    Journal: medRxiv

    Article Title: Mucosal IgA to pre-fusion F protein predicts protection from RSV infection in a high burden setting

    doi: 10.64898/2026.03.16.26348479

    Figure Lengend Snippet: (A) Longitudinal trajectories of serum IgG (top row) and mucosal IgA (bottom row) antibody responses against four RSV-B proteins (PreF, PostF, G, NP) over time. Individual participant trajectories are shown as thin lines with low opacity, colored by infection status: not infected (black), sero-detected infections (orange), and PCR-confirmed infections (green). (B) Mean fold-change in antibody titres between the first bleed (pre-epidemic baseline) and second bleed (post-epidemic) for serum IgG and mucosal IgA responses to RSV-B proteins. Bars represent mean fold-change (log10 scale) stratified by infection status, with error bars indicating standard error.

    Article Snippet: RSV antigens (RSV-A Pre-Fusion (ProteoGenix, product code: PX-P6126), RSV-B Pre-Fusion (SinoBiological, product code: 40832-V08B), RSV-A Post-Fusion (SinoBiological, product code: 11049-V08B), RSV-B Post-Fusion (SinoBiological, product code: 40999-V08H), RSV-A G protein (SinoBiological, product code: 11070-V08H2), RSV-B G protein (SinoBiological, product code: 13029-V08H, RSV-A Nucleoprotein (SinoBiological, product code: 40821-V08E and RSV-B Nucleoprotein (SinoBiological, product code: 40822-V08F)), were coupled to distinct bead regions.

    Techniques: Infection

    (A) Post-infection longitudinal antibody titers for four viral RSV-B proteins (PreF, PostF, G, and NP) across different antibody types; serum IgG and mucosal IgA. Lines show the median posterior predictive fit from the fitted Bayesian model, and the points show the observational titre data, with the size correlating with the sample size for that bin. (B) Peak antibody (x axis) and persistence measured as duration above a 2-fold (left panel) and 4-fold titre rise (right panel) in days. Data points show median posterior values for measurements of four viral RSV-B proteins (PreF, PostF, G, and NP) across different antibody types: serum IgG and mucosal IgA.

    Journal: medRxiv

    Article Title: Mucosal IgA to pre-fusion F protein predicts protection from RSV infection in a high burden setting

    doi: 10.64898/2026.03.16.26348479

    Figure Lengend Snippet: (A) Post-infection longitudinal antibody titers for four viral RSV-B proteins (PreF, PostF, G, and NP) across different antibody types; serum IgG and mucosal IgA. Lines show the median posterior predictive fit from the fitted Bayesian model, and the points show the observational titre data, with the size correlating with the sample size for that bin. (B) Peak antibody (x axis) and persistence measured as duration above a 2-fold (left panel) and 4-fold titre rise (right panel) in days. Data points show median posterior values for measurements of four viral RSV-B proteins (PreF, PostF, G, and NP) across different antibody types: serum IgG and mucosal IgA.

    Article Snippet: RSV antigens (RSV-A Pre-Fusion (ProteoGenix, product code: PX-P6126), RSV-B Pre-Fusion (SinoBiological, product code: 40832-V08B), RSV-A Post-Fusion (SinoBiological, product code: 11049-V08B), RSV-B Post-Fusion (SinoBiological, product code: 40999-V08H), RSV-A G protein (SinoBiological, product code: 11070-V08H2), RSV-B G protein (SinoBiological, product code: 13029-V08H, RSV-A Nucleoprotein (SinoBiological, product code: 40821-V08E and RSV-B Nucleoprotein (SinoBiological, product code: 40822-V08F)), were coupled to distinct bead regions.

    Techniques: Infection

    Serum IgG, top row; mucosal IgA, bottom row and columns are viral antigen target (PreF, PostF, G, and NP for both RSV-A and RSV-B strains). The solid green line represents the mean estimated probability of protection given exposure to infection as a function of antibody titre, with shaded ribbons indicating 95% credible intervals. Background histograms show the distribution of antibody titres at infection for infected individuals (orange) versus non-infected individuals (gray).

    Journal: medRxiv

    Article Title: Mucosal IgA to pre-fusion F protein predicts protection from RSV infection in a high burden setting

    doi: 10.64898/2026.03.16.26348479

    Figure Lengend Snippet: Serum IgG, top row; mucosal IgA, bottom row and columns are viral antigen target (PreF, PostF, G, and NP for both RSV-A and RSV-B strains). The solid green line represents the mean estimated probability of protection given exposure to infection as a function of antibody titre, with shaded ribbons indicating 95% credible intervals. Background histograms show the distribution of antibody titres at infection for infected individuals (orange) versus non-infected individuals (gray).

    Article Snippet: RSV antigens (RSV-A Pre-Fusion (ProteoGenix, product code: PX-P6126), RSV-B Pre-Fusion (SinoBiological, product code: 40832-V08B), RSV-A Post-Fusion (SinoBiological, product code: 11049-V08B), RSV-B Post-Fusion (SinoBiological, product code: 40999-V08H), RSV-A G protein (SinoBiological, product code: 11070-V08H2), RSV-B G protein (SinoBiological, product code: 13029-V08H, RSV-A Nucleoprotein (SinoBiological, product code: 40821-V08E and RSV-B Nucleoprotein (SinoBiological, product code: 40822-V08F)), were coupled to distinct bead regions.

    Techniques: Infection

    Model performance comparison across single biomarker models and the dual biomarker model, defined by out-of-sample predictive accuracy (LOO-ELPD, x-axis) and discrimination ability (area under the ROC curve, AUC, y-axis). Circles indicate serum IgG models, squares represent mucosal IgA models, and the triangle denotes the dual biomarker model combining serum IgG and mucosal IgA to RSV-B PreF. The best-performing model within each biomarker class is highlighted with darker shading. Error bars show the standard error of LOO-ELPD (horizontal) and 95% confidence intervals for AUC (vertical). The dashed horizontal line indicates an AUC of 0.7.

    Journal: medRxiv

    Article Title: Mucosal IgA to pre-fusion F protein predicts protection from RSV infection in a high burden setting

    doi: 10.64898/2026.03.16.26348479

    Figure Lengend Snippet: Model performance comparison across single biomarker models and the dual biomarker model, defined by out-of-sample predictive accuracy (LOO-ELPD, x-axis) and discrimination ability (area under the ROC curve, AUC, y-axis). Circles indicate serum IgG models, squares represent mucosal IgA models, and the triangle denotes the dual biomarker model combining serum IgG and mucosal IgA to RSV-B PreF. The best-performing model within each biomarker class is highlighted with darker shading. Error bars show the standard error of LOO-ELPD (horizontal) and 95% confidence intervals for AUC (vertical). The dashed horizontal line indicates an AUC of 0.7.

    Article Snippet: RSV antigens (RSV-A Pre-Fusion (ProteoGenix, product code: PX-P6126), RSV-B Pre-Fusion (SinoBiological, product code: 40832-V08B), RSV-A Post-Fusion (SinoBiological, product code: 11049-V08B), RSV-B Post-Fusion (SinoBiological, product code: 40999-V08H), RSV-A G protein (SinoBiological, product code: 11070-V08H2), RSV-B G protein (SinoBiological, product code: 13029-V08H, RSV-A Nucleoprotein (SinoBiological, product code: 40821-V08E and RSV-B Nucleoprotein (SinoBiological, product code: 40822-V08F)), were coupled to distinct bead regions.

    Techniques: Comparison, Biomarker Discovery

    (A) Longitudinal trajectories of serum IgG (top row) and mucosal IgA (bottom row) antibody responses against four RSV-B proteins (PreF, PostF, G, NP) over time. Individual participant trajectories are shown as thin lines with low opacity, colored by infection status: not infected (black), sero-detected infections (orange), and PCR-confirmed infections (green). (B) Mean fold-change in antibody titres between the first bleed (pre-epidemic baseline) and second bleed (post-epidemic) for serum IgG and mucosal IgA responses to RSV-B proteins. Bars represent mean fold-change (log10 scale) stratified by infection status, with error bars indicating standard error.

    Journal: medRxiv

    Article Title: Mucosal IgA to pre-fusion F protein predicts protection from RSV infection in a high burden setting

    doi: 10.64898/2026.03.16.26348479

    Figure Lengend Snippet: (A) Longitudinal trajectories of serum IgG (top row) and mucosal IgA (bottom row) antibody responses against four RSV-B proteins (PreF, PostF, G, NP) over time. Individual participant trajectories are shown as thin lines with low opacity, colored by infection status: not infected (black), sero-detected infections (orange), and PCR-confirmed infections (green). (B) Mean fold-change in antibody titres between the first bleed (pre-epidemic baseline) and second bleed (post-epidemic) for serum IgG and mucosal IgA responses to RSV-B proteins. Bars represent mean fold-change (log10 scale) stratified by infection status, with error bars indicating standard error.

    Article Snippet: RSV antigens (RSV-A Pre-Fusion (ProteoGenix, product code: PX-P6126), RSV-B Pre-Fusion (SinoBiological, product code: 40832-V08B), RSV-A Post-Fusion (SinoBiological, product code: 11049-V08B), RSV-B Post-Fusion (SinoBiological, product code: 40999-V08H), RSV-A G protein (SinoBiological, product code: 11070-V08H2), RSV-B G protein (SinoBiological, product code: 13029-V08H, RSV-A Nucleoprotein (SinoBiological, product code: 40821-V08E and RSV-B Nucleoprotein (SinoBiological, product code: 40822-V08F)), were coupled to distinct bead regions.

    Techniques: Infection

    (A) Post-infection longitudinal antibody titers for four viral RSV-B proteins (PreF, PostF, G, and NP) across different antibody types; serum IgG and mucosal IgA. Lines show the median posterior predictive fit from the fitted Bayesian model, and the points show the observational titre data, with the size correlating with the sample size for that bin. (B) Peak antibody (x axis) and persistence measured as duration above a 2-fold (left panel) and 4-fold titre rise (right panel) in days. Data points show median posterior values for measurements of four viral RSV-B proteins (PreF, PostF, G, and NP) across different antibody types: serum IgG and mucosal IgA.

    Journal: medRxiv

    Article Title: Mucosal IgA to pre-fusion F protein predicts protection from RSV infection in a high burden setting

    doi: 10.64898/2026.03.16.26348479

    Figure Lengend Snippet: (A) Post-infection longitudinal antibody titers for four viral RSV-B proteins (PreF, PostF, G, and NP) across different antibody types; serum IgG and mucosal IgA. Lines show the median posterior predictive fit from the fitted Bayesian model, and the points show the observational titre data, with the size correlating with the sample size for that bin. (B) Peak antibody (x axis) and persistence measured as duration above a 2-fold (left panel) and 4-fold titre rise (right panel) in days. Data points show median posterior values for measurements of four viral RSV-B proteins (PreF, PostF, G, and NP) across different antibody types: serum IgG and mucosal IgA.

    Article Snippet: RSV antigens (RSV-A Pre-Fusion (ProteoGenix, product code: PX-P6126), RSV-B Pre-Fusion (SinoBiological, product code: 40832-V08B), RSV-A Post-Fusion (SinoBiological, product code: 11049-V08B), RSV-B Post-Fusion (SinoBiological, product code: 40999-V08H), RSV-A G protein (SinoBiological, product code: 11070-V08H2), RSV-B G protein (SinoBiological, product code: 13029-V08H, RSV-A Nucleoprotein (SinoBiological, product code: 40821-V08E and RSV-B Nucleoprotein (SinoBiological, product code: 40822-V08F)), were coupled to distinct bead regions.

    Techniques: Infection

    Serum IgG, top row; mucosal IgA, bottom row and columns are viral antigen target (PreF, PostF, G, and NP for both RSV-A and RSV-B strains). The solid green line represents the mean estimated probability of protection given exposure to infection as a function of antibody titre, with shaded ribbons indicating 95% credible intervals. Background histograms show the distribution of antibody titres at infection for infected individuals (orange) versus non-infected individuals (gray).

    Journal: medRxiv

    Article Title: Mucosal IgA to pre-fusion F protein predicts protection from RSV infection in a high burden setting

    doi: 10.64898/2026.03.16.26348479

    Figure Lengend Snippet: Serum IgG, top row; mucosal IgA, bottom row and columns are viral antigen target (PreF, PostF, G, and NP for both RSV-A and RSV-B strains). The solid green line represents the mean estimated probability of protection given exposure to infection as a function of antibody titre, with shaded ribbons indicating 95% credible intervals. Background histograms show the distribution of antibody titres at infection for infected individuals (orange) versus non-infected individuals (gray).

    Article Snippet: RSV antigens (RSV-A Pre-Fusion (ProteoGenix, product code: PX-P6126), RSV-B Pre-Fusion (SinoBiological, product code: 40832-V08B), RSV-A Post-Fusion (SinoBiological, product code: 11049-V08B), RSV-B Post-Fusion (SinoBiological, product code: 40999-V08H), RSV-A G protein (SinoBiological, product code: 11070-V08H2), RSV-B G protein (SinoBiological, product code: 13029-V08H, RSV-A Nucleoprotein (SinoBiological, product code: 40821-V08E and RSV-B Nucleoprotein (SinoBiological, product code: 40822-V08F)), were coupled to distinct bead regions.

    Techniques: Infection

    Model performance comparison across single biomarker models and the dual biomarker model, defined by out-of-sample predictive accuracy (LOO-ELPD, x-axis) and discrimination ability (area under the ROC curve, AUC, y-axis). Circles indicate serum IgG models, squares represent mucosal IgA models, and the triangle denotes the dual biomarker model combining serum IgG and mucosal IgA to RSV-B PreF. The best-performing model within each biomarker class is highlighted with darker shading. Error bars show the standard error of LOO-ELPD (horizontal) and 95% confidence intervals for AUC (vertical). The dashed horizontal line indicates an AUC of 0.7.

    Journal: medRxiv

    Article Title: Mucosal IgA to pre-fusion F protein predicts protection from RSV infection in a high burden setting

    doi: 10.64898/2026.03.16.26348479

    Figure Lengend Snippet: Model performance comparison across single biomarker models and the dual biomarker model, defined by out-of-sample predictive accuracy (LOO-ELPD, x-axis) and discrimination ability (area under the ROC curve, AUC, y-axis). Circles indicate serum IgG models, squares represent mucosal IgA models, and the triangle denotes the dual biomarker model combining serum IgG and mucosal IgA to RSV-B PreF. The best-performing model within each biomarker class is highlighted with darker shading. Error bars show the standard error of LOO-ELPD (horizontal) and 95% confidence intervals for AUC (vertical). The dashed horizontal line indicates an AUC of 0.7.

    Article Snippet: RSV antigens (RSV-A Pre-Fusion (ProteoGenix, product code: PX-P6126), RSV-B Pre-Fusion (SinoBiological, product code: 40832-V08B), RSV-A Post-Fusion (SinoBiological, product code: 11049-V08B), RSV-B Post-Fusion (SinoBiological, product code: 40999-V08H), RSV-A G protein (SinoBiological, product code: 11070-V08H2), RSV-B G protein (SinoBiological, product code: 13029-V08H, RSV-A Nucleoprotein (SinoBiological, product code: 40821-V08E and RSV-B Nucleoprotein (SinoBiological, product code: 40822-V08F)), were coupled to distinct bead regions.

    Techniques: Comparison, Biomarker Discovery