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rsv a2 strain  (ATCC)


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    Structured Review

    ATCC rsv a2 strain
    Rsv A2 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rsv+a2/Human+respiratory+syncytial+virus/pm42314330-48-10-13
    Average 97 stars, based on 453 article reviews
    rsv a2 strain - by Bioz Stars, 2026-10
    97/100 stars

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    Incubation:

    Article Title: Immunogenicity of a Recombinant MVA Vector Vaccine Expressing the Prefusion RSV F Protein in Balb/c Mice
    Article Snippet: Diluted test samples and positive control serum were added to a 96-well plate (Gibco, Grand Island, NY, USA, No. 701001). .. Then, 500 plaque-forming units (PFUs) of RSV-A2 (ATCC VR-1540) were added to each well, and the plate was incubated at 37 °C with 5% CO 2 for 1 h. The virus-serum mixture was subsequently transferred to Hep-2 cell monolayers (ATCC CCL-23) that had been cultured at 37 °C with 5% CO 2 overnight. ..

    Virus:

    Article Title: Immunogenicity of a Recombinant MVA Vector Vaccine Expressing the Prefusion RSV F Protein in Balb/c Mice
    Article Snippet: Diluted test samples and positive control serum were added to a 96-well plate (Gibco, Grand Island, NY, USA, No. 701001). .. Then, 500 plaque-forming units (PFUs) of RSV-A2 (ATCC VR-1540) were added to each well, and the plate was incubated at 37 °C with 5% CO 2 for 1 h. The virus-serum mixture was subsequently transferred to Hep-2 cell monolayers (ATCC CCL-23) that had been cultured at 37 °C with 5% CO 2 overnight. ..

    Article Title: Corrected and republished from: "Clinical validation of an RSV neutralization assay and analysis of cross-sectional sera associated with 2021-2023 RSV outbreaks to investigate the immunity debt hypothesis".
    Article Snippet: .. Assays were run with RSV A2 as the challenge virus (ATCC, VR-1516), unless other wise specified. .. RSV B strain WV/14617/85 (ATCC, VR-1400) challenge experiments are described in Supplemental Methods.

    Cell Culture:

    Article Title: Immunogenicity of a Recombinant MVA Vector Vaccine Expressing the Prefusion RSV F Protein in Balb/c Mice
    Article Snippet: Diluted test samples and positive control serum were added to a 96-well plate (Gibco, Grand Island, NY, USA, No. 701001). .. Then, 500 plaque-forming units (PFUs) of RSV-A2 (ATCC VR-1540) were added to each well, and the plate was incubated at 37 °C with 5% CO 2 for 1 h. The virus-serum mixture was subsequently transferred to Hep-2 cell monolayers (ATCC CCL-23) that had been cultured at 37 °C with 5% CO 2 overnight. ..

    Control:

    Article Title: Adenovector 26 Encoded RSV Prefusion F Protein (Ad26.RSV.preF) Does Not Predispose to Enhanced Respiratory Disease in Preclinical Rodent Models
    Article Snippet: Female cotton rats (Sigmovir Biosystems, Inc.; 6–8 weeks old) or mice (Envigo, Indianapolis, IN, USA; 6–8 weeks old) were immunized intramuscularly at Week 0 with formulation buffer (mock), the indicated doses of Ad26.RSV.preF [ ], or at Week 0 and Week 4 with FI-RSV, for which the original lot 100 that induced ERD in clinical studies [ ] was used in the same dilution that was shown to induce ERD in cotton rats (1:125) [ ]. .. A control group received 10 4 pfu live RSV A2 (VR-1540, ATCC, Manassas, VA, USA) or RSV B Wash 18537 (Sigmovir, Biosystems, Inc.) intranasally at Week 0. ..

    Purification:

    Article Title: RSM01, an extended half-life RSV monoclonal antibody with a high barrier to resistance
    Article Snippet: HEp-2 cells (ATCC CCL-23) were passaged in DMEM (Gibco, high glucose, Glutamax, pyruvate, 31966-021) supplemented with 5% fetal bovine serum and pen/strep. .. Master stocks of RSV-A2 (ATCC VR-1540) and RSV-B1 (B WV/14617/85, VR-1400) were triple plaque purified in HEp-2 cells and propagated for a total of 5 and 8 passages, respectively, before use as parental strains. .. Four mAbs were evaluated (Table S1): RSM01 (Gates Medical Research Institute), Palivizumab (Synagis®), research-grade Nirsevimab (MedChemExpress, HY-P99756), and research-grade Clesrovimab (MedChemExpress, HY-P99804).

    other:

    Article Title: Reliability of RSV Antigen Diagnostic Tests in the Presence of Nirsevimab
    Article Snippet: RSV A2 (ATCC VR-1540) and B9320 (ATCC VR-955) were purchased from American Type Culture Collection (Manassas, VA) and propagated before use in Hep-2 (ATCC CCL-23) or Vero cells (ATCC CCL-81), respectively.

    Sterility:

    Article Title: β 2 -adrenergic signaling controls neonatal respiratory syncytial virus infection by promoting viral clearance and airway protection
    Article Snippet: .. Cells were then washed with warm sterile 1X PBS and infected with live RSV-A2 (Cat# VR1540-P, ATCC) together with PBS or albuterol for 4 hours. ..

    Infection:

    Article Title: β 2 -adrenergic signaling controls neonatal respiratory syncytial virus infection by promoting viral clearance and airway protection
    Article Snippet: .. Cells were then washed with warm sterile 1X PBS and infected with live RSV-A2 (Cat# VR1540-P, ATCC) together with PBS or albuterol for 4 hours. ..

    Neutralization:

    Article Title: Mucosal immunization with an adenoviral vector expressing a prefusogenic F protein protects the upper and lower respiratory tracts of cotton rats against live respiratory syncytial virus challenge
    Article Snippet: The RSV Long strain (ATCC, VR-26) and HEp-2 cells (ATCC CCL-23) used for neutralization assays in the mouse study were obtained from the National Institute for Viral Disease Control and Prevention, China CDC. .. Neutralization assays in cotton rats were performed using the following strains: RSV A2 (ATCC VR-1540), RSV B (strain 18537; ATCC VR-1580PQ), and RSV Long (ATCC VR-26). .. These viruses were propagated in human epithelial type 2 (HEp-2) cells (ATCC CCL-23) by Sigmovir Biosystems (Rockville, MD).



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    rsv a2  (ATCC)
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    ATCC wild type rsv a2 vr 1540
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    Synergistic activities of clinical antiviral remdesivir and HS mimetic Tet-N3 <t>against</t> <t>RSV-A2</t> in a plaque reduction assay. Panel (A) IC 50 and CC 50 of Tet-N3 against RSV infection in HEp-2 cells; (B) IC 50 of RDV against RSV infection in HEp-2 cells, (C) Bliss analysis graph, and (D) heatmap of combinational treatment of Tet-N3 and RDV against RSV plaque formation, with a mean synergistic combination score of 28.01. Panels A and B show the means ± SD from triplicate data points from three independent biological replicates. Panels C and D show the mean data from triplicate data points.
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    Synergistic activities of clinical antiviral remdesivir and HS mimetic Tet-N3 <t>against</t> <t>RSV-A2</t> in a plaque reduction assay. Panel (A) IC 50 and CC 50 of Tet-N3 against RSV infection in HEp-2 cells; (B) IC 50 of RDV against RSV infection in HEp-2 cells, (C) Bliss analysis graph, and (D) heatmap of combinational treatment of Tet-N3 and RDV against RSV plaque formation, with a mean synergistic combination score of 28.01. Panels A and B show the means ± SD from triplicate data points from three independent biological replicates. Panels C and D show the mean data from triplicate data points.
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    Image Search Results


    Synergistic activities of clinical antiviral remdesivir and HS mimetic Tet-N3 against RSV-A2 in a plaque reduction assay. Panel (A) IC 50 and CC 50 of Tet-N3 against RSV infection in HEp-2 cells; (B) IC 50 of RDV against RSV infection in HEp-2 cells, (C) Bliss analysis graph, and (D) heatmap of combinational treatment of Tet-N3 and RDV against RSV plaque formation, with a mean synergistic combination score of 28.01. Panels A and B show the means ± SD from triplicate data points from three independent biological replicates. Panels C and D show the mean data from triplicate data points.

    Journal: ACS Omega

    Article Title: Antiviral Activity of Dendritic Heparan Sulfate Mimetics against Respiratory Syncytial Virus and Herpes Simplex Virus‑1

    doi: 10.1021/acsomega.5c12846

    Figure Lengend Snippet: Synergistic activities of clinical antiviral remdesivir and HS mimetic Tet-N3 against RSV-A2 in a plaque reduction assay. Panel (A) IC 50 and CC 50 of Tet-N3 against RSV infection in HEp-2 cells; (B) IC 50 of RDV against RSV infection in HEp-2 cells, (C) Bliss analysis graph, and (D) heatmap of combinational treatment of Tet-N3 and RDV against RSV plaque formation, with a mean synergistic combination score of 28.01. Panels A and B show the means ± SD from triplicate data points from three independent biological replicates. Panels C and D show the mean data from triplicate data points.

    Article Snippet: Wild-type RSV-A2 (VR-1540) was obtained from ATCC.

    Techniques: Infection