rsv a2 strain (ATCC)

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ATCC rsv a2 strain
Rsv A2 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rsv+a/Human+respiratory+syncytial+virus/pm42314330-48-10-13
Average 97 stars, based on 450 article reviews

rsv a2 strain - by Bioz Stars, 2026-09

97/100 stars

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Quantitative RT-PCR:

Article Title: Reliability of RSV Antigen Diagnostic Tests in the Presence of Nirsevimab
Article Snippet: One-step reverse transcriptase quantitative PCR (RT-qPCR) was subsequently conducted utilizing AgPATH-ID One-Step RT-PCR Reagents (4387424, Applied Biosystems, Foster City, CA) and previously described RSV-specific primer and probe sequences.12 Reactions were run in duplicate with a total volume of 25 μL comprising 12.5 μL of 2X RT-PCR Buffer, 1 μL 25X RT-PCR Enzyme Mix, 5 μL extracted vRNA, and 500 nM of each primer and 150 nM fluorescein amidite-labeled probe. .. RT-qPCR was conducted on an Applied Biosystems (Foster City, CA) QuantStudio 7 Flex with thermal cycler conditions of 50°C for 2 minutes, 60°C for 20 minutes, 95°C for 5 minutes and 45 cycles of 94°C for 20 seconds and 60°C for 1 minute.12 Quantitative genomic RNA from RSV A (ATCC VR-1540DQ) and RSV B (ATCC VR-1580DQ) was used to generate a standard curve for the RT-qPCR assay, allowing the observed cycle threshold values from the specimen pools to be converted into genome copies/mL using linear regression. ..

Neutralization:

Article Title: Respiratory Syncytial Virus Vaccination in Allogeneic Hematopoietic Stem Cell Transplant Recipients
Article Snippet: .. Neutralization assays used RSV-A (starin VR26 ATCC) infecting HEp-2 cells in the presence of serial dilutions of serum (1:4 to 1:2048). ..

Incubation:

Article Title: Metavac-RSV mucosal bivalent vaccine candidate protects cotton rats against pneumoviruses and is produced using serum-free cell culture in bioreactor
Article Snippet: .. Diluted samples were incubated with 25-50 PFU of RSV A (RSV A2, lot #102313 SSM), RSV B (RSV B 18537, ATCC VR-1580, lot #032417 SSM), or HMPV A (HMPV TN/94-49/A2, lot #030116 SSM) for 1 h at room temperature and inoculated in duplicates onto confluent HEp-2 for RSV, or LLC-MK2 for HMPV. ..

Modification:

Article Title:
Article Snippet: .. SimplexaTM Flu A/B & RSV Direct Gen II Analytical Reactivity with Flu B Strains Lineage Organism Tested Concentration1 Victoria B/Brisbane/33/2008 20 CEID50/mL Victoria B/Brisbane/60/2008 20 CEID50/mL Victoria B/Florida/02/2006 100 TCID50/mL Victoria B/Lee/40 100 TCID50/mL Victoria B/Nevada/03/2011 100 CEID50/mL Victoria B/Texas/02/2013 100 TCID50/mL Victoria B/Victoria/304/2006 50 CEID50/mL Yamagata B/Christchurch/33/2004 100 CEID50/mL Yamagata B/Florida/07/04 100 TCID50/mL Yamagata B/Florida/04/2006 100 TCID50/mL Yamagata B/Guangdong-Liwan/1133/2014 400 CEID50/mL Yamagata B/Maryland/1/59 100 TCID50/mL Unknown B/Great Lakes/1739/54 100 TCID50/mL Yamagata B/Panama/45/90 100 TCID50/mL Yamagata B/Phuket/3073/2013 200 CEID50/mL Yamagata B/Utah/09/2014 100 CEID50/mL K201505 - Page 21 of 26 Lineage Organism Tested Concentration1 Yamagata B/Wisconsin/01/2010 100 CEID50/mL Unknown B/Allen/45 100 TCID50/mL Unknown B/Hong Kong/5/72 100 TCID50/mL Unknown B/Taiwan/2/62 100 TCID50/mL Victoria B/Colorado/06/2017 20 TCID50/mL Victoria B/Michigan/09/2011 5 EID50/mL Yamagata B/Texas/81/2016 20 EID50/mL Victoria B/Washington/02/2019 200 EID50/mL Yamagata B/New Hampshire/01/2016 100 EID50/mL 1 TCID50/mL = Tissue Culture Infectious Dose CEID50/mL = Chicken Embryo Infectious Dose EID50/mL = Egg Infectious Dose Simplexa Flu A/B & RSV Direct Gen II Analytical Reactivity with RSV Strains Organism Tested Concentration1 RSV ATCC-2012-10 100 PFU/mL RSV A 1997/12-35 100 TCID50/mL RSV A 1998/12-21 100 TCID50/mL RSV A 1998/3-2 100 TCID50/mL RSV A 2000/3-4 100 TCID50/mL RSV A 2001/2-20 100 TCID50/mL RSV A 2001/3-12 100 TCID50/mL RSV A Long 100 TCID50/mL RSV B 9320 100 TCID50/mL RSV B/Wash/18537/62 100 TCID50/mL RSV B/WV/14617/85 100 TCID50/mL 1 TCID50/mL = Tissue Culture Infectious Dose PFU/mL = Plaque Forming Units Additionally, testing of eight contemporary human influenza viruses was conducted to compare the reactivity of the modified device (Simplexa Flu A/B & RSV Direct Gen II) to the unmodified version (Simplexa Flu A/B & RSV Direct). ..

Derivative Assay:

Article Title:
Article Snippet: .. The results below summarize the findings from a reproducibility study on replicates of 4 simulated samples containing viral material from culture derived isolates: Flu A-H1 (strain A/WS/33 (H1N1), ATCC VR-1520), RSV-A (ATCC VR-26), Adenovirus (Species C, Serotype 5, strain Adenoid 75, ATCC VR-5). ..

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