cell lines rs4 (ATCC)
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Cell Lines Rs4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 548 article reviews
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Control:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and In Vivo:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and Injection:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and Flow Cytometry:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and MANN-WHITNEY:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and Inhibition:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and Incubation:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and Biomarker Discovery:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and Western Blot:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and Cytotoxicity Assay:Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells Article Snippet: Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.Cell line authentication was performed by PCR amplification of nine short tandem repeat (STR) loci (Research Animal Diagnostic Laboratory, St. Louis, MO) and comparing the profile to the ATCC STR database.. The STR profile of MCF-7 and |
![Fig. 1. Decitabine (DEC)-induced effects on methyltransferase activity, gene and protein expression. (A) 3D crystal protein structure of DNMT1 (gray) in complex with DNA (red). The DNA-binding CXXC domain is colored in green. RCSB Protein data bank PDB identifier 3PTA [6,74]. (B) Crystal structure of the KMT2A CXXC domain (green) bound to human DNA (red). RCSB Protein data bank PDB identifier 2KKF [75,76]. (C) Amino acid (aa) sequences of the DNMT1 (top), and KMT2A (bottom) CXXC domains were retrieved from uniprot [77]. Shared amino acids were analyzed using BLASTP suite-2sequences [78] search, and depicted in the middle box. (D–G) Acute B-lymphoblastic leukemia cell lines <t>SEM,</t> <t>RS4;11,</t> REH, and NALM-6 were incubated with 1 μM DEC or vehicle substance (DMSO) for 72 h. (D) Influence on global methylation was analyzed by methylation-specific qPCR of the LINE-1 retrotransposon. n = 3, mean SD; paired t test. (E) Quantitative analysis of histone 3, lysine residue 4 trimethylation (H3K4me3) by western blot was used to determine the global transcription rate. n = 3, mean SD; ratio paired t test of absolute GAPDH-normalized signal intensities. (F) Probe-based gene expression analysis of DNMT1, and KMT2A. Both, the N-terminal CXXC-domain as well as the C-terminal SET domain have been analyzed. n = 3, mean SD; paired t test of ΔCt vs. GAPDH values. (G) Quantitative analysis of DNMT1, and KMT2A protein expression. n = 3, mean SD; ratio paired t test of absolute GAPDH-normalized signal intensities. *P < 0.05; **P < 0.01.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_4404/pm39754404/pm39754404__page5_image1.jpg)