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h ro15 4513  (Revvity)


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    Structured Review

    Revvity h ro15 4513
    H Ro15 4513, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ro15+4513/RO+15-4513/pmc10154722-75-21-22
    Average 91 stars, based on 14 article reviews
    h ro15 4513 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Incubation:

    Article Title: Erbb4 deletion from fast-spiking interneurons causes psychosis-relevant neuroimaging phenotypes
    Article Snippet: .. To quantify density of α5GABA A R – sections were incubated for 60 minutes at room temperature in 2nM [ 3 H]Ro15-4513 (Perkin Elmer, NET925250UC), or in 2nM [ 3 H]Ro15-4513 with 10 μM bretazenil (Sigma, B6434) for nonspecific binding. .. To quantify α1-3;5GABA A R sections were incubated for 60 minutes at 4°C in 1nM [ 3 H]flumazenil (Perkin Elmer, NET757001MC), or in 1nM [ 3 H]flumazenil with 10 μM flunitrazepam (Sigma Aldrich, F-907 1ML) for nonspecific binding.

    Article Title: Structural Requirements for Eszopiclone and Zolpidem Binding to the γ-Aminobutyric Acid Type-A (GABAA) Receptor Are Different
    Article Snippet: .. Cells were harvested and membrane homogenates prepared 48 h post-transfection as described.49 Briefly, membrane homogenates (50 μg) were incubated at room temperature for 40 min with a sub-Kd concentration of radioligand ([3H]Ro15-1788, 70.7Ci/mmol; [3H]flunitrazepam, 85.2 Ci/mmol; [3H]Ro15-4513, 35.7 Ci/mmol; PerkinElmer Life and Analytical Sciences, Boston, MA) in the absence or presence of seven different concentrations of unlabeled ligand in a final volume of 250 μL. .. Data were fit by nonlinear regression to a one-site competition curve defined by the equation y ) Bmax/[1 + (x/IC50)], where y is bound [3H]ligand in disintegrations per minute, Bmax is maximal binding, x is the concentration of displacing ligand, and IC50 is the concentration of unlabeled ligand that inhibits 50% of [3H]ligand binding (Prism, GraphPad Software).

    Binding Assay:

    Article Title: Erbb4 deletion from fast-spiking interneurons causes psychosis-relevant neuroimaging phenotypes
    Article Snippet: .. To quantify density of α5GABA A R – sections were incubated for 60 minutes at room temperature in 2nM [ 3 H]Ro15-4513 (Perkin Elmer, NET925250UC), or in 2nM [ 3 H]Ro15-4513 with 10 μM bretazenil (Sigma, B6434) for nonspecific binding. .. To quantify α1-3;5GABA A R sections were incubated for 60 minutes at 4°C in 1nM [ 3 H]flumazenil (Perkin Elmer, NET757001MC), or in 1nM [ 3 H]flumazenil with 10 μM flunitrazepam (Sigma Aldrich, F-907 1ML) for nonspecific binding.

    Membrane:

    Article Title: Structural Requirements for Eszopiclone and Zolpidem Binding to the γ-Aminobutyric Acid Type-A (GABAA) Receptor Are Different
    Article Snippet: .. Cells were harvested and membrane homogenates prepared 48 h post-transfection as described.49 Briefly, membrane homogenates (50 μg) were incubated at room temperature for 40 min with a sub-Kd concentration of radioligand ([3H]Ro15-1788, 70.7Ci/mmol; [3H]flunitrazepam, 85.2 Ci/mmol; [3H]Ro15-4513, 35.7 Ci/mmol; PerkinElmer Life and Analytical Sciences, Boston, MA) in the absence or presence of seven different concentrations of unlabeled ligand in a final volume of 250 μL. .. Data were fit by nonlinear regression to a one-site competition curve defined by the equation y ) Bmax/[1 + (x/IC50)], where y is bound [3H]ligand in disintegrations per minute, Bmax is maximal binding, x is the concentration of displacing ligand, and IC50 is the concentration of unlabeled ligand that inhibits 50% of [3H]ligand binding (Prism, GraphPad Software).

    Concentration Assay:

    Article Title: Structural Requirements for Eszopiclone and Zolpidem Binding to the γ-Aminobutyric Acid Type-A (GABAA) Receptor Are Different
    Article Snippet: .. Cells were harvested and membrane homogenates prepared 48 h post-transfection as described.49 Briefly, membrane homogenates (50 μg) were incubated at room temperature for 40 min with a sub-Kd concentration of radioligand ([3H]Ro15-1788, 70.7Ci/mmol; [3H]flunitrazepam, 85.2 Ci/mmol; [3H]Ro15-4513, 35.7 Ci/mmol; PerkinElmer Life and Analytical Sciences, Boston, MA) in the absence or presence of seven different concentrations of unlabeled ligand in a final volume of 250 μL. .. Data were fit by nonlinear regression to a one-site competition curve defined by the equation y ) Bmax/[1 + (x/IC50)], where y is bound [3H]ligand in disintegrations per minute, Bmax is maximal binding, x is the concentration of displacing ligand, and IC50 is the concentration of unlabeled ligand that inhibits 50% of [3H]ligand binding (Prism, GraphPad Software).



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    ro15 4513  (Revvity)
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    Revvity ro15 4513
    Representative hippocampal regions of interests. (A) Regions of interests sampled for [ 3 <t>H]Ro15-4513</t> and [ 3 H]flumazenil. (B) Regions of interests sampled for [ 3 H]UCB-J. dHip CA1: dorsal hippocampus CA1; mHip CA3: middle hippocampus CA3; mHip CA1/2: middle hippocampus CA1/CA2; vHip CA3: ventral hippocampus CA3; dHip DG: dorsal hippocampus dentate gyrus; mHip DG: middle hippocampus dentate gyrus.
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    ro15  (Tocris)
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    Representative hippocampal regions of interests. (A) Regions of interests sampled for [ 3 <t>H]Ro15-4513</t> and [ 3 H]flumazenil. (B) Regions of interests sampled for [ 3 H]UCB-J. dHip CA1: dorsal hippocampus CA1; mHip CA3: middle hippocampus CA3; mHip CA1/2: middle hippocampus CA1/CA2; vHip CA3: ventral hippocampus CA3; dHip DG: dorsal hippocampus dentate gyrus; mHip DG: middle hippocampus dentate gyrus.
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    Image Search Results


    Representative hippocampal regions of interests. (A) Regions of interests sampled for [ 3 H]Ro15-4513 and [ 3 H]flumazenil. (B) Regions of interests sampled for [ 3 H]UCB-J. dHip CA1: dorsal hippocampus CA1; mHip CA3: middle hippocampus CA3; mHip CA1/2: middle hippocampus CA1/CA2; vHip CA3: ventral hippocampus CA3; dHip DG: dorsal hippocampus dentate gyrus; mHip DG: middle hippocampus dentate gyrus.

    Journal: bioRxiv

    Article Title: Erbb4 deletion from fast-spiking interneurons causes psychosis-relevant neuroimaging phenotypes

    doi: 10.1101/2022.03.07.483347

    Figure Lengend Snippet: Representative hippocampal regions of interests. (A) Regions of interests sampled for [ 3 H]Ro15-4513 and [ 3 H]flumazenil. (B) Regions of interests sampled for [ 3 H]UCB-J. dHip CA1: dorsal hippocampus CA1; mHip CA3: middle hippocampus CA3; mHip CA1/2: middle hippocampus CA1/CA2; vHip CA3: ventral hippocampus CA3; dHip DG: dorsal hippocampus dentate gyrus; mHip DG: middle hippocampus dentate gyrus.

    Article Snippet: To quantify density of α5GABA A R – sections were incubated for 60 minutes at room temperature in 2nM [ 3 H]Ro15-4513 (Perkin Elmer, NET925250UC), or in 2nM [ 3 H]Ro15-4513 with 10 µM bretazenil (Sigma, B6434) for nonspecific binding.

    Techniques:

    [ 3 H]UCB-J, but not [ 3 H]Ro15-4513 or [ 3 H]flumazenil binding in Lhx6-Cre;Erbb4 F/F mice is decreased across the hippocampus. (A) [ 3 H]UCB-J showed a significant decrease in synaptic density in Lhx6-Cre;Erbb4 F/F mice (n=11) compared to control animals (n=9) across all hippocampal ROIs (mixed effects analysis; main effect of genotype: F (1,18) =7.27, p =0.02, ). (B) [ 3 H]Ro15-4513 (n=10-11/group) and (C) [ 3 H]flumazenil (n=9-10/group) binding did not differ by genotype. dHip CA1: dorsal hippocampus CA1; mHip CA3: middle hippocampus CA3; mHip CA1/2: middle hippocampus CA1/CA2; vHip CA3: ventral hippocampus CA3; dHip DG: dorsal hippocampus dentate gyrus; mHip DG: dentate gyrus.

    Journal: bioRxiv

    Article Title: Erbb4 deletion from fast-spiking interneurons causes psychosis-relevant neuroimaging phenotypes

    doi: 10.1101/2022.03.07.483347

    Figure Lengend Snippet: [ 3 H]UCB-J, but not [ 3 H]Ro15-4513 or [ 3 H]flumazenil binding in Lhx6-Cre;Erbb4 F/F mice is decreased across the hippocampus. (A) [ 3 H]UCB-J showed a significant decrease in synaptic density in Lhx6-Cre;Erbb4 F/F mice (n=11) compared to control animals (n=9) across all hippocampal ROIs (mixed effects analysis; main effect of genotype: F (1,18) =7.27, p =0.02, ). (B) [ 3 H]Ro15-4513 (n=10-11/group) and (C) [ 3 H]flumazenil (n=9-10/group) binding did not differ by genotype. dHip CA1: dorsal hippocampus CA1; mHip CA3: middle hippocampus CA3; mHip CA1/2: middle hippocampus CA1/CA2; vHip CA3: ventral hippocampus CA3; dHip DG: dorsal hippocampus dentate gyrus; mHip DG: dentate gyrus.

    Article Snippet: To quantify density of α5GABA A R – sections were incubated for 60 minutes at room temperature in 2nM [ 3 H]Ro15-4513 (Perkin Elmer, NET925250UC), or in 2nM [ 3 H]Ro15-4513 with 10 µM bretazenil (Sigma, B6434) for nonspecific binding.

    Techniques: Binding Assay