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Partek genomics suite rna-seq analysis pipeline
( A – B ) Lineage tracing of chondrocytes coexpressing mutant Ifitm5 and Ai9 reporter in Rosa 26 mIfitm5/Ai9 Acan-Cre ERT2 mice. Tamoxifen injection was performed at P10, and bones were collected after 3 weeks (at 5 weeks of age). Representative images of the proximal tibia ( A ) and the tibia diaphyseal area ( B ) Decreased fraction of Ai9 + cells migrated to the bone diaphysis in mutant animals compared with littermate controls ( n = 3 per genotype; n = 2–3 fields counted per sample; * P = 0.03, by 2-tailed Student’s t test). Original magnification, ×20. ( C ) <t>RNA-Seq</t> of total femoral cDNA from Rosa26 mIfitm5/+ Acan-Cre ERT2 mice ( n = 3 per genotype) showed enrichment for chondrogenic gene expression in the mutant samples by GO term analysis. ( D ) Heatmap of focused differential gene expression analysis from RNA-Seq, showing upregulation of chondrogenic gene markers and decreased expression of osteogenic markers in the mutants compared with the control group.
Genomics Suite Rna Seq Analysis Pipeline, supplied by Partek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna-seq+analysis+pipeline/rna+seq+analysis+pipeline/pmc11290974-214-22-21
Average 90 stars, based on 1 article reviews
genomics suite rna-seq analysis pipeline - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect"

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect

Journal: The Journal of Clinical Investigation

doi: 10.1172/JCI170369

( A – B ) Lineage tracing of chondrocytes coexpressing mutant Ifitm5 and Ai9 reporter in Rosa 26 mIfitm5/Ai9 Acan-Cre ERT2 mice. Tamoxifen injection was performed at P10, and bones were collected after 3 weeks (at 5 weeks of age). Representative images of the proximal tibia ( A ) and the tibia diaphyseal area ( B ) Decreased fraction of Ai9 + cells migrated to the bone diaphysis in mutant animals compared with littermate controls ( n = 3 per genotype; n = 2–3 fields counted per sample; * P = 0.03, by 2-tailed Student’s t test). Original magnification, ×20. ( C ) RNA-Seq of total femoral cDNA from Rosa26 mIfitm5/+ Acan-Cre ERT2 mice ( n = 3 per genotype) showed enrichment for chondrogenic gene expression in the mutant samples by GO term analysis. ( D ) Heatmap of focused differential gene expression analysis from RNA-Seq, showing upregulation of chondrogenic gene markers and decreased expression of osteogenic markers in the mutants compared with the control group.
Figure Legend Snippet: ( A – B ) Lineage tracing of chondrocytes coexpressing mutant Ifitm5 and Ai9 reporter in Rosa 26 mIfitm5/Ai9 Acan-Cre ERT2 mice. Tamoxifen injection was performed at P10, and bones were collected after 3 weeks (at 5 weeks of age). Representative images of the proximal tibia ( A ) and the tibia diaphyseal area ( B ) Decreased fraction of Ai9 + cells migrated to the bone diaphysis in mutant animals compared with littermate controls ( n = 3 per genotype; n = 2–3 fields counted per sample; * P = 0.03, by 2-tailed Student’s t test). Original magnification, ×20. ( C ) RNA-Seq of total femoral cDNA from Rosa26 mIfitm5/+ Acan-Cre ERT2 mice ( n = 3 per genotype) showed enrichment for chondrogenic gene expression in the mutant samples by GO term analysis. ( D ) Heatmap of focused differential gene expression analysis from RNA-Seq, showing upregulation of chondrogenic gene markers and decreased expression of osteogenic markers in the mutants compared with the control group.

Techniques Used: Mutagenesis, Injection, RNA Sequencing Assay, Expressing, Control

Related Articles

Expressing:

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: Targeting TGF- β for treatment of osteogenesis imperfecta
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Mutagenesis:

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: Targeting TGF- β for treatment of osteogenesis imperfecta
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Injection:

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: Targeting TGF- β for treatment of osteogenesis imperfecta
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

RNA Sequencing Assay:

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: Targeting TGF- β for treatment of osteogenesis imperfecta
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Control:

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using the RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).

Article Title: Targeting TGF- β for treatment of osteogenesis imperfecta
Article Snippet: Normalization, differential expression, hierarchical clustering, and GO analysis were then performed using RNA-Seq analysis pipeline in Partek Genomics Suite (Partek).



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( A – B ) Lineage tracing of chondrocytes coexpressing mutant Ifitm5 and Ai9 reporter in Rosa 26 mIfitm5/Ai9 Acan-Cre ERT2 mice. Tamoxifen injection was performed at P10, and bones were collected after 3 weeks (at 5 weeks of age). Representative images of the proximal tibia ( A ) and the tibia diaphyseal area ( B ) Decreased fraction of Ai9 + cells migrated to the bone diaphysis in mutant animals compared with littermate controls ( n = 3 per genotype; n = 2–3 fields counted per sample; * P = 0.03, by 2-tailed Student’s t test). Original magnification, ×20. ( C ) <t>RNA-Seq</t> of total femoral cDNA from Rosa26 mIfitm5/+ Acan-Cre ERT2 mice ( n = 3 per genotype) showed enrichment for chondrogenic gene expression in the mutant samples by GO term analysis. ( D ) Heatmap of focused differential gene expression analysis from RNA-Seq, showing upregulation of chondrogenic gene markers and decreased expression of osteogenic markers in the mutants compared with the control group.
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Image Search Results


Counts per million (CPM) for the indicated transcripts from the RNAseq analysis in . ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Journal: bioRxiv

Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

doi: 10.64898/2026.03.10.710866

Figure Lengend Snippet: Counts per million (CPM) for the indicated transcripts from the RNAseq analysis in . ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Article Snippet: Duplicate RNA samples from independent crosses were submitted for each genotype to the Plasmidsaurus RNAseq analysis pipeline.

Techniques: RNA sequencing

A) Table summarizing the overall count of differentially regulated genes in RNAseq analyses performed in isolated fat bodies where the indicated RNAi lines are driven by Dcg-GAL4 . B) Venn-diagram showing the commonalities in the differentially downregulated (left) and upregulated genes (right) in A. C-E) Counts per million (CPM) for the indicated transcripts from the RNAseq analysis in A . C and D show data for known EcR targets, E shows data for known ATF4 targets. Also see Table S1 and S2 . ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Journal: bioRxiv

Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

doi: 10.64898/2026.03.10.710866

Figure Lengend Snippet: A) Table summarizing the overall count of differentially regulated genes in RNAseq analyses performed in isolated fat bodies where the indicated RNAi lines are driven by Dcg-GAL4 . B) Venn-diagram showing the commonalities in the differentially downregulated (left) and upregulated genes (right) in A. C-E) Counts per million (CPM) for the indicated transcripts from the RNAseq analysis in A . C and D show data for known EcR targets, E shows data for known ATF4 targets. Also see Table S1 and S2 . ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Article Snippet: Duplicate RNA samples from independent crosses were submitted for each genotype to the Plasmidsaurus RNAseq analysis pipeline.

Techniques: RNA sequencing, Isolation

( A – B ) Lineage tracing of chondrocytes coexpressing mutant Ifitm5 and Ai9 reporter in Rosa 26 mIfitm5/Ai9 Acan-Cre ERT2 mice. Tamoxifen injection was performed at P10, and bones were collected after 3 weeks (at 5 weeks of age). Representative images of the proximal tibia ( A ) and the tibia diaphyseal area ( B ) Decreased fraction of Ai9 + cells migrated to the bone diaphysis in mutant animals compared with littermate controls ( n = 3 per genotype; n = 2–3 fields counted per sample; * P = 0.03, by 2-tailed Student’s t test). Original magnification, ×20. ( C ) RNA-Seq of total femoral cDNA from Rosa26 mIfitm5/+ Acan-Cre ERT2 mice ( n = 3 per genotype) showed enrichment for chondrogenic gene expression in the mutant samples by GO term analysis. ( D ) Heatmap of focused differential gene expression analysis from RNA-Seq, showing upregulation of chondrogenic gene markers and decreased expression of osteogenic markers in the mutants compared with the control group.

Journal: The Journal of Clinical Investigation

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect

doi: 10.1172/JCI170369

Figure Lengend Snippet: ( A – B ) Lineage tracing of chondrocytes coexpressing mutant Ifitm5 and Ai9 reporter in Rosa 26 mIfitm5/Ai9 Acan-Cre ERT2 mice. Tamoxifen injection was performed at P10, and bones were collected after 3 weeks (at 5 weeks of age). Representative images of the proximal tibia ( A ) and the tibia diaphyseal area ( B ) Decreased fraction of Ai9 + cells migrated to the bone diaphysis in mutant animals compared with littermate controls ( n = 3 per genotype; n = 2–3 fields counted per sample; * P = 0.03, by 2-tailed Student’s t test). Original magnification, ×20. ( C ) RNA-Seq of total femoral cDNA from Rosa26 mIfitm5/+ Acan-Cre ERT2 mice ( n = 3 per genotype) showed enrichment for chondrogenic gene expression in the mutant samples by GO term analysis. ( D ) Heatmap of focused differential gene expression analysis from RNA-Seq, showing upregulation of chondrogenic gene markers and decreased expression of osteogenic markers in the mutants compared with the control group.

Article Snippet: Differential gene expression analysis was performed using DESeq2, and statistical significance was determined by 2-way ANOVA, which is built into the Partek Genomics Suite RNA-Seq analysis pipeline.

Techniques: Mutagenesis, Injection, RNA Sequencing Assay, Expressing, Control