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rna sequencing procedures  (Novogene)

 
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    Novogene rna sequencing procedures
    Rna Sequencing Procedures, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+sequencing+procedures/rna+sequencing/pm41751513-75-4-10
    Average 86 stars, based on 1 article reviews
    rna sequencing procedures - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    RNA Sequencing:

    Article Title: Developmental Nicotine Exposure Induces Intergenerational Transmission of an Ensemble of Neurodevelopmental Disorder-Related Translatomic Perturbations in DRD1-Expressing Striatal Cells of Adolescent Male Mice.
    Article Snippet: .. After sample pooling, all RNA sequencing procedures were performed by Novogene (Sacramento, CA, USA) as described hereafter. ..

    Article Title: Dietary protein defends lean mass and maintains the metabolic benefits of glucagon receptor agonism in mice
    Article Snippet: RNA was quantified using a NanoDrop spectrophotometer. .. RNA sequencing procedures were performed by NovoGene UK. .. Sequencing libraries were prepared using the NGS RNA Library Prep Set (PT042, Novogene).

    Article Title: Dietary protein defends lean mass and maintains the metabolic benefits of glucagon receptor agonism in mice.
    Article Snippet: RNA was quantified using a NanoDrop spectrophotometer. .. RNA sequencing procedures were performed by NovoGene UK. .. Sequencing libraries were prepared using the NGS RNA Library Prep Set (PT042, Novogene).

    Article Title: Developmental Nicotine Exposure Induces Intergenerational Transmission of an Ensemble of Neurodevelopmental Disorder-Related Translatomic Perturbations in DRD1-Expressing Striatal Cells of Adolescent Male Mice
    Article Snippet: .. After sample pooling, all RNA sequencing procedures were performed by Novogene (Sacramento, CA, USA) as described hereafter. ..

    Article Title: Focal Adhesion Regulation as a Strategy against Kidney Fibrosis.
    Article Snippet: Chronic kidney fibrosis poses a significant global health challenge with effective therapeutic strategies remaining elusive.. While cell− extracellular matrix (ECM) interactions are known to drive fibrosis progression, the specific role of focal adhesions (FAs) in kidney fibrosis is not fully understood.. In this study, we investigated the role of FAs in kidney tubular epithelial cell fibrosis by employing precise nanogold patterning to modulate integrin distribution.

    Article Title: Comparative Transcriptome Analysis Reveals the Light Spectra Affect the Growth and Molting of Scylla paramamosain by Changing the Chitin Metabolism.
    Article Snippet: Light is an essential ecological factor that has been demonstrated to affect aquatic animals’ behavior, growth performance, and energy metabolism.. Our previous study found that the full-spectrum light and cyan light could promote growth performance and molting frequency of Scylla paramamosain while it was suppressed by violet light.. Hence, the purpose of this study is to investigate the underlying molecular mechanism that influences light spectral composition on the growth performance and molting of S. paramamosain.

    Generated:

    Article Title: Focal Adhesion Regulation as a Strategy against Kidney Fibrosis.
    Article Snippet: Chronic kidney fibrosis poses a significant global health challenge with effective therapeutic strategies remaining elusive.. While cell− extracellular matrix (ECM) interactions are known to drive fibrosis progression, the specific role of focal adhesions (FAs) in kidney fibrosis is not fully understood.. In this study, we investigated the role of FAs in kidney tubular epithelial cell fibrosis by employing precise nanogold patterning to modulate integrin distribution.

    Gene Expression:

    Article Title: Focal Adhesion Regulation as a Strategy against Kidney Fibrosis.
    Article Snippet: Chronic kidney fibrosis poses a significant global health challenge with effective therapeutic strategies remaining elusive.. While cell− extracellular matrix (ECM) interactions are known to drive fibrosis progression, the specific role of focal adhesions (FAs) in kidney fibrosis is not fully understood.. In this study, we investigated the role of FAs in kidney tubular epithelial cell fibrosis by employing precise nanogold patterning to modulate integrin distribution.



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    CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of bulk RNA-seq data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.

    Journal: Frontiers in Immunology

    Article Title: CD32b defines distinct dendritic cell lineages generated from the culture of bone marrow with GM-CSF

    doi: 10.3389/fimmu.2026.1703978

    Figure Lengend Snippet: CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of bulk RNA-seq data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.

    Article Snippet: RNA sequencing (RNA-seq) procedure and analysis were performed by Macrogen (Seoul, Korea).

    Techniques: Derivative Assay, RNA Sequencing, Generated, Expressing, Biomarker Discovery, Marker, Fluorescence, Control

    Differentially expressed genes (DEG) detected by RNA-seq. (A) Total RNA-sequencing on Het-1A cells pre-treated with 1% DMSO (control) or fexuprazan (test, FXZ, 30 µM) followed by HCl treatment. (B) Top ten functional categories of DEGs. (C) Volcano plot of cluster analysis for DEGs between immune response and inflammatory response of RNA-seq. (D) Heatmap of pyroptosis-related DEGs on Het-1A cells pre-treated with 1% DMSO (DMSO+HCl_1, DMSO+HCl_2) or fexuprazan (FXZ+HCl_1, FXZ +HCl_2).

    Journal: Frontiers in Immunology

    Article Title: Fexuprazan safeguards the esophagus from hydrochloric acid-induced damage by suppressing NLRP1/Caspase-1/GSDMD pyroptotic pathway

    doi: 10.3389/fimmu.2024.1410904

    Figure Lengend Snippet: Differentially expressed genes (DEG) detected by RNA-seq. (A) Total RNA-sequencing on Het-1A cells pre-treated with 1% DMSO (control) or fexuprazan (test, FXZ, 30 µM) followed by HCl treatment. (B) Top ten functional categories of DEGs. (C) Volcano plot of cluster analysis for DEGs between immune response and inflammatory response of RNA-seq. (D) Heatmap of pyroptosis-related DEGs on Het-1A cells pre-treated with 1% DMSO (DMSO+HCl_1, DMSO+HCl_2) or fexuprazan (FXZ+HCl_1, FXZ +HCl_2).

    Article Snippet: Total RNA sequencing procedures were performed by Ebiogen, Inc. (Seoul, Korea).

    Techniques: RNA Sequencing, Control, Functional Assay