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total rna isolation and microarray analysis  (Hokkaido System Science Co)

 
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    Hokkaido System Science Co total rna isolation and microarray analysis
    Total Rna Isolation And Microarray Analysis, supplied by Hokkaido System Science Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray/total+rna+isolation+and+microarray+analysis/pm40632689-92-4-9
    Average 90 stars, based on 1 article reviews
    total rna isolation and microarray analysis - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Enhanced CXCL12/CXCR4 signaling increases tumor progression in radiation‑resistant pancreatic cancer.
    Article Snippet: The mRNA microarray experi‐ ments were performed by Hokkaido System Science Co., Ltd.

    Microarray:

    Article Title: The potent tumor suppressor miR-497 inhibits cancer phenotypes in nasopharyngeal carcinoma by targeting ANLN and HSPA4L
    Article Snippet: RNA containing miRNA was extracted using the mirVanaTM miRNA Isolation Kit (Ambion, USA). .. One hundred nanograms of total RNA from seven NPC biopsies and five NNE samples were submitted to the Agilent miRNA microarray analysis service (Hokkaido System Science Co., Ltd., Sapporo, Japan). ..

    Article Title: Enhanced CXCL12/CXCR4 signaling increases tumor progression in radiation-resistant pancreatic cancer
    Article Snippet: Total mRNA from normal and radiation-resistant MIA PaCa-2 cells was isolated using the RNeasy Plus Mini kit (Qiagen, Inc.) according to the manufacturer's instructions. .. The mRNA microarray experiments were performed by Hokkaido System Science Co., Ltd. .. Transcripts amplified from total mRNA were hybridized to a SurePrint G3 Human 8×60K v3 array (Agilent Technologies, Inc.) according to the manufacturer's protocol.

    Article Title: Overexpression of Ephrin A2 receptors in cancer stromal cells is a prognostic factor for the relapse of gastric cancer.
    Article Snippet: Real-time RT-PCR analysis were performed according manufacture’s protocol (Power SYBR Green PCR, applied biosystems, Warrington, UK) using Ephrin primers (Supplementary Table 1) by ABI SDS-7900HT (ABI). .. For RNA Microarray analysis, synthesis of cRNA, hybridization, scanning and data analysis were performed by Hokkaido System Science Co., Ltd. (Sapporo, Japan). .. Briefly, cyanine-3 (Cy3) labeled cRNA was prepared from total RNA (0.05 lg) using the Low Input Quick Amp Labeling Kit (Agilent) according to the manufacturer’s instructions.

    Article Title: The potent tumor suppressor miR-497 inhibits cancer phenotypes in nasopharyngeal carcinoma by targeting ANLN and HSPA4L
    Article Snippet: .. Fifty nanograms of RNA from seven NPC biopsies and five NNE samples were subjected to Agilent SurePrint G3 Human GE microarray analysis (Hokkaido System Science Co., Ltd.). .. The array (SurePrint G3 Human GE 8 × 60K, 1 color) contained probes for more than 20,000 genes for gene expression analysis.

    Hybridization:

    Article Title: Overexpression of Ephrin A2 receptors in cancer stromal cells is a prognostic factor for the relapse of gastric cancer.
    Article Snippet: Real-time RT-PCR analysis were performed according manufacture’s protocol (Power SYBR Green PCR, applied biosystems, Warrington, UK) using Ephrin primers (Supplementary Table 1) by ABI SDS-7900HT (ABI). .. For RNA Microarray analysis, synthesis of cRNA, hybridization, scanning and data analysis were performed by Hokkaido System Science Co., Ltd. (Sapporo, Japan). .. Briefly, cyanine-3 (Cy3) labeled cRNA was prepared from total RNA (0.05 lg) using the Low Input Quick Amp Labeling Kit (Agilent) according to the manufacturer’s instructions.



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    Volcano plots showing the mature miRNAs differentially expressed in the TraxE126A mutants compared to wildtype littermates as identified by (A) miRNA sequencing and (B) miRNA <t>microarray.</t> Volcano plots showing the differentially expressed <t>small</t> <t>RNA</t> species identified using microarray analysis including (C) Precursor miRNAs (pre-miRNAs), (D) Small nucleolar RNAs (snoRNAs), (E) mature tRNAs and (F) tRNA-derived <t>small</t> <t>RNAs</t> (tsRNAs). In all the plots, the upregulated and downregulated miRNAs (false discovery rate, FDR <0.050 and log 2 fold change ≥0.200) are highlighted in red and blue, respectively. (TraxE126A, n=4; WT, n=5, all males). Largest changes were seen in tsRNA levels (majority are 5’-fragments) and mature miRNAs.
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    Volcano plots showing the mature miRNAs differentially expressed in the TraxE126A mutants compared to wildtype littermates as identified by (A) miRNA sequencing and (B) miRNA <t>microarray.</t> Volcano plots showing the differentially expressed <t>small</t> <t>RNA</t> species identified using microarray analysis including (C) Precursor miRNAs (pre-miRNAs), (D) Small nucleolar RNAs (snoRNAs), (E) mature tRNAs and (F) tRNA-derived <t>small</t> <t>RNAs</t> (tsRNAs). In all the plots, the upregulated and downregulated miRNAs (false discovery rate, FDR <0.050 and log 2 fold change ≥0.200) are highlighted in red and blue, respectively. (TraxE126A, n=4; WT, n=5, all males). Largest changes were seen in tsRNA levels (majority are 5’-fragments) and mature miRNAs.
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    Volcano plots showing the mature miRNAs differentially expressed in the TraxE126A mutants compared to wildtype littermates as identified by (A) miRNA sequencing and (B) miRNA <t>microarray.</t> Volcano plots showing the differentially expressed <t>small</t> <t>RNA</t> species identified using microarray analysis including (C) Precursor miRNAs (pre-miRNAs), (D) Small nucleolar RNAs (snoRNAs), (E) mature tRNAs and (F) tRNA-derived <t>small</t> <t>RNAs</t> (tsRNAs). In all the plots, the upregulated and downregulated miRNAs (false discovery rate, FDR <0.050 and log 2 fold change ≥0.200) are highlighted in red and blue, respectively. (TraxE126A, n=4; WT, n=5, all males). Largest changes were seen in tsRNA levels (majority are 5’-fragments) and mature miRNAs.
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    Image Search Results


    Volcano plots showing the mature miRNAs differentially expressed in the TraxE126A mutants compared to wildtype littermates as identified by (A) miRNA sequencing and (B) miRNA microarray. Volcano plots showing the differentially expressed small RNA species identified using microarray analysis including (C) Precursor miRNAs (pre-miRNAs), (D) Small nucleolar RNAs (snoRNAs), (E) mature tRNAs and (F) tRNA-derived small RNAs (tsRNAs). In all the plots, the upregulated and downregulated miRNAs (false discovery rate, FDR <0.050 and log 2 fold change ≥0.200) are highlighted in red and blue, respectively. (TraxE126A, n=4; WT, n=5, all males). Largest changes were seen in tsRNA levels (majority are 5’-fragments) and mature miRNAs.

    Journal: bioRxiv

    Article Title: Genetic inactivation of the Translin/Trax RNase activity alters small RNAs including miRNAs, disrupts gene expression and impairs distinct forms of hippocampal synaptic plasticity and memory

    doi: 10.1101/2025.07.10.663777

    Figure Lengend Snippet: Volcano plots showing the mature miRNAs differentially expressed in the TraxE126A mutants compared to wildtype littermates as identified by (A) miRNA sequencing and (B) miRNA microarray. Volcano plots showing the differentially expressed small RNA species identified using microarray analysis including (C) Precursor miRNAs (pre-miRNAs), (D) Small nucleolar RNAs (snoRNAs), (E) mature tRNAs and (F) tRNA-derived small RNAs (tsRNAs). In all the plots, the upregulated and downregulated miRNAs (false discovery rate, FDR <0.050 and log 2 fold change ≥0.200) are highlighted in red and blue, respectively. (TraxE126A, n=4; WT, n=5, all males). Largest changes were seen in tsRNA levels (majority are 5’-fragments) and mature miRNAs.

    Article Snippet: The labeled RNA species are then hybridized onto Arraystar Small RNA Expression Microarray (8×15K format), scanned by an Agilent G2505C scanner followed by data processing and analysis.

    Techniques: Sequencing, Microarray, Derivative Assay

    (A) Venn diagram showing the overlap between mature miRNAs identified using miRNA sequencing and microarray analysis (with FDR<0.050 and log 2 fold change ≥0.200). A total of 12 miRNAs (10 upregulated and 2 downregulated) were found to be common and were used for target prediction using miRDB database. (B) An upset plot showing the shared and unique predicted mRNA target profiles in the miRDB database for the 12 common miRNAs. Only targets with miRDB Target Score ≥60 are included. (C) Top 15 KEGG pathways and (D) Gene Ontology (GO) Biological Process terms from the functional enrichment analysis of the predicted targets of the 12 common miRNAs performed using DAVID database.

    Journal: bioRxiv

    Article Title: Genetic inactivation of the Translin/Trax RNase activity alters small RNAs including miRNAs, disrupts gene expression and impairs distinct forms of hippocampal synaptic plasticity and memory

    doi: 10.1101/2025.07.10.663777

    Figure Lengend Snippet: (A) Venn diagram showing the overlap between mature miRNAs identified using miRNA sequencing and microarray analysis (with FDR<0.050 and log 2 fold change ≥0.200). A total of 12 miRNAs (10 upregulated and 2 downregulated) were found to be common and were used for target prediction using miRDB database. (B) An upset plot showing the shared and unique predicted mRNA target profiles in the miRDB database for the 12 common miRNAs. Only targets with miRDB Target Score ≥60 are included. (C) Top 15 KEGG pathways and (D) Gene Ontology (GO) Biological Process terms from the functional enrichment analysis of the predicted targets of the 12 common miRNAs performed using DAVID database.

    Article Snippet: The labeled RNA species are then hybridized onto Arraystar Small RNA Expression Microarray (8×15K format), scanned by an Agilent G2505C scanner followed by data processing and analysis.

    Techniques: Sequencing, Microarray, Functional Assay