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rlight  (R&D Systems)


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    R&D Systems rlight
    Rlight, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rlight/product/R&D Systems
    Average 94 stars, based on 22 article reviews
    rlight - by Bioz Stars, 2026-05
    94/100 stars

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    a mRNA level of TGF-β1 in RAW264.7 cells after treatment with rLIGHT for various times. b Protein levels of P-JNK and JNK in RAW264.7 cells after treatment with rLIGHT. c mRNA level of TGF-β1 in RAW264.7 cells after blocking LIGHT in splenocytes. d Protein level of TGF-β1 in RAW264.7 cells after blocking LIGHT in splenocytes. mRNA expression was examined by qPCR and normalized to GAPDH expression. The relative grayscale value of the protein band was measured with ImageJ software and normalized to β-actin. All data (means ± SEM) were obtained from triplicate experiments (one-way ANOVA with Tukey’s multiple comparison test, * P < 0.05, ** P < 0.01).

    Journal: Experimental & Molecular Medicine

    Article Title: Splenectomy improves liver fibrosis via tumor necrosis factor superfamily 14 (LIGHT) through the JNK/TGF-β1 signaling pathway

    doi: 10.1038/s12276-021-00574-2

    Figure Lengend Snippet: a mRNA level of TGF-β1 in RAW264.7 cells after treatment with rLIGHT for various times. b Protein levels of P-JNK and JNK in RAW264.7 cells after treatment with rLIGHT. c mRNA level of TGF-β1 in RAW264.7 cells after blocking LIGHT in splenocytes. d Protein level of TGF-β1 in RAW264.7 cells after blocking LIGHT in splenocytes. mRNA expression was examined by qPCR and normalized to GAPDH expression. The relative grayscale value of the protein band was measured with ImageJ software and normalized to β-actin. All data (means ± SEM) were obtained from triplicate experiments (one-way ANOVA with Tukey’s multiple comparison test, * P < 0.05, ** P < 0.01).

    Article Snippet: The concentration of both the anti-TGF-β1-neutralizing antibody (Cat. No.: MAB1835-SP, R&D Systems) and rLIGHT protein (Cat. No.: 1794-LT/CF, R&D Systems) was 100 ng/ml.

    Techniques: Blocking Assay, Expressing, Software, Comparison

    a The levels of TGF-β1, P-JNK, and JNK in RAW264.7 cells. b The levels of TGF-β1 in the culture medium of JS1 cells. c The levels of αSMA in JS1 cells. d Immunofluorescence staining of αSMA and quantitative analysis of αSMA-positive cells among JS1 cells. Magnification: ×400. Control (RAW264.7 cells), rLIGHT (RAW264.7 cells + rLIGHT), rLIGHT + anti-P-JNK (RAW264.7 cells + rLIGHT + anti-P-JNK). Scale bar, 50 µm. mRNA expression was examined by qPCR and normalized to GAPDH expression. The relative grayscale value of the protein band was measured with ImageJ software and normalized to β-actin. The data (means ± SEM) were obtained from triplicate experiments (one-way ANOVA with Tukey’s multiple comparison test, * P < 0.05, ** P < 0.01, and *** P < 0.001).

    Journal: Experimental & Molecular Medicine

    Article Title: Splenectomy improves liver fibrosis via tumor necrosis factor superfamily 14 (LIGHT) through the JNK/TGF-β1 signaling pathway

    doi: 10.1038/s12276-021-00574-2

    Figure Lengend Snippet: a The levels of TGF-β1, P-JNK, and JNK in RAW264.7 cells. b The levels of TGF-β1 in the culture medium of JS1 cells. c The levels of αSMA in JS1 cells. d Immunofluorescence staining of αSMA and quantitative analysis of αSMA-positive cells among JS1 cells. Magnification: ×400. Control (RAW264.7 cells), rLIGHT (RAW264.7 cells + rLIGHT), rLIGHT + anti-P-JNK (RAW264.7 cells + rLIGHT + anti-P-JNK). Scale bar, 50 µm. mRNA expression was examined by qPCR and normalized to GAPDH expression. The relative grayscale value of the protein band was measured with ImageJ software and normalized to β-actin. The data (means ± SEM) were obtained from triplicate experiments (one-way ANOVA with Tukey’s multiple comparison test, * P < 0.05, ** P < 0.01, and *** P < 0.001).

    Article Snippet: The concentration of both the anti-TGF-β1-neutralizing antibody (Cat. No.: MAB1835-SP, R&D Systems) and rLIGHT protein (Cat. No.: 1794-LT/CF, R&D Systems) was 100 ng/ml.

    Techniques: Immunofluorescence, Staining, Control, Expressing, Software, Comparison

    a The level of αSMA in JS1 cells after treatment with an anti-TGF-β1-neutralizing antibody. b Immunofluorescence staining of αSMA in JS1 cells after treatment with an anti-TGF-β1-neutralizing antibody. Magnification: ×400. Control (coculture assay of RAW264.7 and JS1 cells); rLIGHT group: (coculture assay of RAW264.7 and JS1 cells with rLIGHT stimulation); rLIGHT+TGF-β1NAB: (coculture assay of RAW264.7 and JS1 cells treated with rLIGHT and an anti-TGF-β1-neutralizing antibody); scale bar, 50 µm. The relative grayscale value of the protein band was measured with ImageJ software and normalized to β-actin. The data (means ± SEM) were obtained from triplicate experiments (one-way ANOVA with Tukey’s multiple comparison test, * P < 0.05 and ** P < 0.01).

    Journal: Experimental & Molecular Medicine

    Article Title: Splenectomy improves liver fibrosis via tumor necrosis factor superfamily 14 (LIGHT) through the JNK/TGF-β1 signaling pathway

    doi: 10.1038/s12276-021-00574-2

    Figure Lengend Snippet: a The level of αSMA in JS1 cells after treatment with an anti-TGF-β1-neutralizing antibody. b Immunofluorescence staining of αSMA in JS1 cells after treatment with an anti-TGF-β1-neutralizing antibody. Magnification: ×400. Control (coculture assay of RAW264.7 and JS1 cells); rLIGHT group: (coculture assay of RAW264.7 and JS1 cells with rLIGHT stimulation); rLIGHT+TGF-β1NAB: (coculture assay of RAW264.7 and JS1 cells treated with rLIGHT and an anti-TGF-β1-neutralizing antibody); scale bar, 50 µm. The relative grayscale value of the protein band was measured with ImageJ software and normalized to β-actin. The data (means ± SEM) were obtained from triplicate experiments (one-way ANOVA with Tukey’s multiple comparison test, * P < 0.05 and ** P < 0.01).

    Article Snippet: The concentration of both the anti-TGF-β1-neutralizing antibody (Cat. No.: MAB1835-SP, R&D Systems) and rLIGHT protein (Cat. No.: 1794-LT/CF, R&D Systems) was 100 ng/ml.

    Techniques: Immunofluorescence, Staining, Control, Co-culture Assay, Software, Comparison

    a Gross images of liver tissues. b F4/80+ and F4/80+/TGF-β1 + macrophages were analyzed by immunofluorescence staining. Ratios were calculated (magnification ×400). c Sirius red staining and immunohistochemical staining of αSMA in the liver tissues in each group (magnification ×400 and ×200). d The levels of TGF-β1 and αSMA in the various groups. Normal mice (Blank), ConA-treated mice (ConA); ConA + splenectomy-treated mice (splenectomy); ConA + splenectomy + rLIGHT-treated mice (rLIGHT). In the animal studies, n = 6/group. In all panels, scale bars = 50 µm. The relative grayscale value of the protein band was measured with ImageJ software and normalized to β-actin. The data (means ± SEM) were obtained from triplicate experiments (one-way ANOVA with Tukey’s multiple comparison test, * P < 0.05, ** P < 0.01).

    Journal: Experimental & Molecular Medicine

    Article Title: Splenectomy improves liver fibrosis via tumor necrosis factor superfamily 14 (LIGHT) through the JNK/TGF-β1 signaling pathway

    doi: 10.1038/s12276-021-00574-2

    Figure Lengend Snippet: a Gross images of liver tissues. b F4/80+ and F4/80+/TGF-β1 + macrophages were analyzed by immunofluorescence staining. Ratios were calculated (magnification ×400). c Sirius red staining and immunohistochemical staining of αSMA in the liver tissues in each group (magnification ×400 and ×200). d The levels of TGF-β1 and αSMA in the various groups. Normal mice (Blank), ConA-treated mice (ConA); ConA + splenectomy-treated mice (splenectomy); ConA + splenectomy + rLIGHT-treated mice (rLIGHT). In the animal studies, n = 6/group. In all panels, scale bars = 50 µm. The relative grayscale value of the protein band was measured with ImageJ software and normalized to β-actin. The data (means ± SEM) were obtained from triplicate experiments (one-way ANOVA with Tukey’s multiple comparison test, * P < 0.05, ** P < 0.01).

    Article Snippet: The concentration of both the anti-TGF-β1-neutralizing antibody (Cat. No.: MAB1835-SP, R&D Systems) and rLIGHT protein (Cat. No.: 1794-LT/CF, R&D Systems) was 100 ng/ml.

    Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Software, Comparison