ril 17a  (R&D Systems)

Journal: Journal of Animal Science and Biotechnology

Article Title: Baicalin alleviates mastitis in dairy cows by targeting IL-17RA to inhibit IL-17 signaling pathway activation

doi: 10.1186/s40104-026-01401-2

Figure Lengend Snippet: Schematic overview of baicalin's mechanism in alleviating mastitis by regulating the IL-17RA-mediated IL-17 signaling pathway. A Oral administration of baicalin to mice and dairy cows. B In vivo release of LPS by E. coli . C LPS activated the TLR4/MyD88/NF-κB pathway in epithelial cells. D NF-κB was activated within the cell nucleus. E E. coli infection increases IL-17A content in mammary glands. F IL-17A binds to IL-17RA on the cell membrane surface, thereby activating the IL-17 signaling pathway. G Activation of the MAPK signaling pathway and ERK signaling pathway. H Production of IL-6, TNFα and IL-1β. I TNFα activated TNF signaling pathway. J TJ structure damage. K Baicalin inhibits IL-17RA signal transduction, thereby preventing activation of the IL-17 signaling pathway

Article Snippet: An in vitro mastitis model was established by treating cells with 5 μg/mL LPS (Sigma, USA) for 12 h. Baicalin (purity ≥ 95%) was purchased from Macklin (Shanghai, China), with a concentration of 20 μmol/L for 24 h. Furthermore, the TNFα inhibitor SPD304 and recombinant IL-17A (rIL-17A) were purchased from MedChemExpress (MCE, USA) and were used at concentrations of 5 μmol/L (for 2 h) and 100 ng/mL (for 12 h), respectively.

Techniques: In Vivo, Infection, Membrane, Activation Assay, Transduction

Journal: Nature Communications

Article Title: Antibody Fc-receptor FcεR1γ stabilizes cell surface receptors in group 3 innate lymphoid cells and promotes anti-infection immunity

doi: 10.1038/s41467-024-50266-4

Figure Lengend Snippet: A Experimental scheme of C. rodentium infection model ( Methods ). B Representative periodic acid–Schiff (PAS) staining images of colons of Rorc-cre – Fcer1g f/f and Rorc-cre + Fcer1g f/f mice on day 7 post the infection as in ( A ). C Quantification of the histological scores of images as in ( B ) ( n = 5 mice per group). D, E Representative images of bacterial load determined by bioluminescent imaging in the colon ( D ) and the quantification ( E ) ( n = 6 mice per group). F-I Representative flow plots showing the intracellular abundance of IL-22 ( F ) or IL-17A ( H ) in total, CCR6 + and CCR6 – ILC3s from siLPs. Quantification of the frequencies of IL-22 ( G, Rorc-cre – Fcer1g f/f , n = 11 mice; Rorc-cre + Fcer1g f/f , n = 8 mice) or IL-17A ( I, Rorc-cre – Fcer1g f/f , n = 11 mice; Rorc-cre + Fcer1g f/f , n = 8 mice) -expressing cells in each compartment. Data are representative of two ( B-E, F, H ) or are pooled from two ( G, I ) independent experiments shown as the mean ± SEM. Statistical significance was tested by two-tailed t test ( C, E, G, I ).

Article Snippet: Mice were injected intraperitoneally with a dose of 1 μg rIL-17A protein (R&D Systems) or 0.8 μg rIL-22 protein (R&D Systems) 6 h before C. albicans infection, followed by a booster dose of the same cytokine at 24 h after infection.

Techniques: Infection, Staining, Imaging, Expressing, Two Tailed Test

Journal: Nature Communications

Article Title: Antibody Fc-receptor FcεR1γ stabilizes cell surface receptors in group 3 innate lymphoid cells and promotes anti-infection immunity

doi: 10.1038/s41467-024-50266-4

Figure Lengend Snippet: A Heatmap showing the expression (color bar, Z score) of genes (rows) significantly induced in ILC3s by the fungal infection (Supplementary Fig. ) across different genotypes and conditions (columns) ( Rorc-cre – Fcer1g f/f PBS, n = 4 mice; Rorc-cre + Fcer1g f/f C.A ., n = 4 mice; Rorc-cre – Fcer1g f/f C.A ., n = 3 mice). C.A . represents Candida albicans . B Quantification of the overall expression ( Methods ) of the genes in ( A ). C Quantification of total, CCR6 + and CCR6 – ILC3s in the siLPs of Rorc-cre − Fcer1g f/f and Rorc-cre + Fcer1g f/f mice on day 5 post the infection ( n = 6 mice per group). D-G Representative flow plots showing the intracellular abundance of IL-22 ( D ) or IL-17A ( F ) in total, CCR6 + and CCR6 – ILC3s on day 5 post the infection. Quantification of the frequencies of IL-22 ( E, Rorc-cre – Fcer1g f/f , n = 7 mice; Rorc-cre + Fcer1g f/f , n = 6 mice) or IL-17A ( G, Rorc-cre – Fcer1g f/f , n = 7 mice; Rorc-cre + Fcer1g f/f , n = 6 mice) -expressing cells in each compartment. H, I Representative flow plots showing the phosphorylation of JAK1, JAK2 and JAK3 in total ILC3s from siLPs of indicated mice on day 5 post the C. albicans infection ( H ). Quantification of MFI ( I, Rorc-cre – Fcer1g f/f , n = 5 mice; Rorc-cre + Fcer1g f/f , n = 4 mice). Data are representative of two ( C – G ) or one ( H , I ) or from two ( A , B ) independent experiments shown as the mean ± SEM. Statistical significance was tested by two-tailed t test ( B, C, E, G, I ).

Article Snippet: Mice were injected intraperitoneally with a dose of 1 μg rIL-17A protein (R&D Systems) or 0.8 μg rIL-22 protein (R&D Systems) 6 h before C. albicans infection, followed by a booster dose of the same cytokine at 24 h after infection.

Techniques: Expressing, Infection, Two Tailed Test

Journal: Nature Communications

Article Title: Antibody Fc-receptor FcεR1γ stabilizes cell surface receptors in group 3 innate lymphoid cells and promotes anti-infection immunity

doi: 10.1038/s41467-024-50266-4

Figure Lengend Snippet: A Experimental scheme. B Survival curves of C. albicans- infected Rorc-cre + Fcer1g f/f mice treated with IL-17A, IL-22 or vehicle (PBS) ( Rorc-cre – Fcer1g f/f PBS, n = 18 mice; Rorc-cre + Fcer1g f/f PBS, n = 21 mice; Rorc-cre + Fcer1g f/f IL-22, n = 13 mice; Rorc-cre + Fcer1g f/f IL-17A, n = 11 mice). C Quantification of the fungi load in kidneys of mice as in ( A ) on day 5 post the infection ( Rorc-cre – Fcer1g f/f PBS, n = 18 mice; Rorc-cre + Fcer1g f/f PBS, n = 16 mice; Rorc-cre + Fcer1g f/f IL-22, n = 7 mice; Rorc-cre + Fcer1g f/f IL-17A, n = 11 mice). D, E Representative PAS staining images of kidneys of mice as in ( A ) on day 5 post the infection. Boxes: medulla area with mycelia, showing in higher magnification in ( E ). F Quantification of IL-17A in the serum of mice as in Fig. on day 5 post the infection. n = 6 mice per group. Data are representative of three ( D, E ) or one ( F ) or are pooled from four ( B ) or three ( C ) independent experiments shown as the mean ± SEM. Statistical significance was tested by two-tailed t test ( C, F ) or log-rank (Mantel-Cox) test ( B ).

Article Snippet: Mice were injected intraperitoneally with a dose of 1 μg rIL-17A protein (R&D Systems) or 0.8 μg rIL-22 protein (R&D Systems) 6 h before C. albicans infection, followed by a booster dose of the same cytokine at 24 h after infection.

Techniques: Infection, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: Antibody Fc-receptor FcεR1γ stabilizes cell surface receptors in group 3 innate lymphoid cells and promotes anti-infection immunity

doi: 10.1038/s41467-024-50266-4

Figure Lengend Snippet: A, B Representative flow plots showing the intracellular abundance of IL-17A ( A ) in total, CCR6 + and CCR6 – ILC3s from siLPs of mice with the indicated genotypes on day 5 post C. albicans infection. Quantification of the frequencies of IL-17A-expressing cells in each compartment ( B , WT, n = 7 mice; Ncr1 –/– , n = 5 mice; Fcgr3 –/– , n = 4 mice). C Survival curves of C. albicans- infected Rag1 −/− Rorc-cre − Fcer1g f/f ( n = 8 mice) and Rag1 −/− Rorc-cre + Fcer1g f/f mice ( n = 7 mice). All mice were intravenously infected with C. albicans on day 0 ( Methods ). D Survival curves of C. albicans- infected Rag1 −/− Rorc-cre − Fcer1g f/f and Rag1 −/− Rorc-cre + Fcer1g f/f mice treated with mIgG ( Methods ) ( n = 8 mice per group). E, F Representative flow plots showing the intracellular abundance of IL-17A ( E ) in total, CCR6 + and CCR6 – ILC3s from siLPs of mice in ( D ) on day 5 post the infection. Quantification of the frequencies of IL-17A ( F )-expressing cells in each compartment ( n = 4 mice per group). Data are pooled from three ( A, B ) or representative of three ( C ) or two ( D-F ) independent experiments shown as the mean ± SEM. Statistical significance was tested by two-tailed t test ( B, F ) or log-rank (Mantel-Cox) test ( C, D ).

Article Snippet: Mice were injected intraperitoneally with a dose of 1 μg rIL-17A protein (R&D Systems) or 0.8 μg rIL-22 protein (R&D Systems) 6 h before C. albicans infection, followed by a booster dose of the same cytokine at 24 h after infection.

Techniques: Infection, Expressing, Two Tailed Test