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antigapdh hfab rhodamine antibody  (Bio-Rad)


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    Structured Review

    Bio-Rad antigapdh hfab rhodamine antibody
    Antigapdh Hfab Rhodamine Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhodamine+hfab/hFAB+Rhodamine+Anti-GAPDH+Primary+Antibody/pm42901169-112-14-18
    Average 95 stars, based on 103 article reviews
    antigapdh hfab rhodamine antibody - by Bioz Stars, 2026-11
    95/100 stars

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    Incubation:

    Article Title: Pharmacological Blockade of LPAR1 Does Not Provide Neuroprotection in a Rat Model of Ocular Hypertensive Glaucoma
    Article Snippet: Blots were blocked with 5% non-fat milk (nfm) in Tris-buffered saline (TBS; cat. no. 1706435; Bio-Rad Life Science) for 90 minutes and subsequently incubated with primary antibody (1:1000 dilution; cat. no. SAB5702205; Sigma-Aldrich Corp., St. Louis, MO, USA) diluted in 5% nfm in TBS-T (TBS supplemented with 0.1% Tween-20; cat. no. 1706531; Bio-Rad Life Science) overnight at 4°C (ON). .. After four extensive TBS-T washes of five minutes each, the blot was incubated with HRP conjugated secondary antibody for one hour (1:5000 dilution; cat. no. P039901-2; Agilent Technologies, Winooski, VT, USA) together with actin conjugated with rhodamine hFAB (used as loading control, 1:5000 dilution, cat. no. 12004167; BioRad Laboratories) at room temperature in 5% nfm in TBS-T. After five washes of five minutes each in TBS-T, the blot was rinsed in TBS and proteins signals acquired. .. The signal was detected by chemiluminescence (Clarity, cat. no. 1705060; Bio-Rad Life Science) for EDG2, while loading the control by fluorescence signal.

    Control:

    Article Title: Pharmacological Blockade of LPAR1 Does Not Provide Neuroprotection in a Rat Model of Ocular Hypertensive Glaucoma
    Article Snippet: Blots were blocked with 5% non-fat milk (nfm) in Tris-buffered saline (TBS; cat. no. 1706435; Bio-Rad Life Science) for 90 minutes and subsequently incubated with primary antibody (1:1000 dilution; cat. no. SAB5702205; Sigma-Aldrich Corp., St. Louis, MO, USA) diluted in 5% nfm in TBS-T (TBS supplemented with 0.1% Tween-20; cat. no. 1706531; Bio-Rad Life Science) overnight at 4°C (ON). .. After four extensive TBS-T washes of five minutes each, the blot was incubated with HRP conjugated secondary antibody for one hour (1:5000 dilution; cat. no. P039901-2; Agilent Technologies, Winooski, VT, USA) together with actin conjugated with rhodamine hFAB (used as loading control, 1:5000 dilution, cat. no. 12004167; BioRad Laboratories) at room temperature in 5% nfm in TBS-T. After five washes of five minutes each in TBS-T, the blot was rinsed in TBS and proteins signals acquired. .. The signal was detected by chemiluminescence (Clarity, cat. no. 1705060; Bio-Rad Life Science) for EDG2, while loading the control by fluorescence signal.



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    Image Search Results


    Reagents and tools table

    Journal: EMBO Reports

    Article Title: Vimentin loss inhibits DNA damage responses and promotes cancer cell survival

    doi: 10.1038/s44319-026-00913-2

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Anti-actin hFAB Rhodamine antibody , Bio-Rad , 12004163.

    Techniques: Cell-Signaling, Sequencing