j gleeson  (Addgene inc)

Journal: The Journal of Biological Chemistry

Article Title: Mice Lacking ?-Tubulin Acetyltransferase 1 Are Viable but Display ?-Tubulin Acetylation Deficiency and Dentate Gyrus Distortion *

doi: 10.1074/jbc.M113.464792

Figure Lengend Snippet: Human ATAT1 colocalizes with doublecortin-bundled microtubules. A, HEK293 cells were transfected with an expression plasmid for GFP-α-tubulin, RFP-DCX, or GFP-ATAT1 for live green or red fluorescence microscopy. B, HEK293 cells were cotransfected with the expression plasmids for GFP-α-tubulin and RFP-DCX for live green and red fluorescence microscopy. Cross-talk between different channels was none or minimal (data not shown). C, same as B except that GFP-ATAT1 was expressed instead of GFP-α-tubulin. Similar results were obtained with mCherry-ATAT1 and GFP-DCX, although mCherry-ATAT1 was more enriched in the cytoplasm than GFP-ATAT1 (data not shown). Scale bar, 40 μm.

Article Snippet: The eGFP-ATAT1 construct was prepared from a human cDNA clone from Open Biosystems, whereas the ones for eGFP-α-tubulin and RFP-DCX were obtained from Addgene (plasmids 30387 and 32851, respectively).

Techniques: Transfection, Expressing, Plasmid Preparation, Fluorescence, Microscopy